Abstract:
:Single-strand breaks (SSB) are discontinuities in one strand of the DNA double helix and are the most common type of damages that arise in cells. SSBs arise mainly from direct attack by intracellular metabolites, however, also essential nuclear processes generate SSBs as intermediates. During the catalytic cycle of DNA topoisomerase I (Top1) a SSB is generated, which is normally transient and rapidly resealed by the enzyme. However, several situations can stabilize a Top1-generated SSB, and this poses the risk of converting the SSB into a double strand break (DSB) if encountered by the replication machinery. A DSB is a more serious treat for cells as it can fuel chromosomal rearrangements and thus jeopardize genome stability and cause cells to become cancerous. In this perspective, we discuss the cellular consequences of Top1-generated damage during DNA replication with focus on the differences between endogenous Top1-generated damage and Top1 damage generated due to the use of the drug camptothecin.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Jakobsen KP,Andersen AH,Bjergbæk Ldoi
10.1007/s00294-019-00984-wsubject
Has Abstractpub_date
2019-10-01 00:00:00pages
1141-1144issue
5eissn
0172-8083issn
1432-0983pii
10.1007/s00294-019-00984-wjournal_volume
65pub_type
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