Abstract:
:We describe a new nuclear gene, CBT1 (Cytochrome B Termination), specifically involved in the generation of mature mRNA of cytochrome b in yeast mitochondria. Disruption of CBT1 (corresponding to ORF YKL 208W) results in a respiratory deficiency (no growth on acetate and ethanol, a reduced growth on glycerol, and a moderate growth on lactate). Cytochrome b is practically undetectable spectrally, while cytochromes a and a3 (cytochrome oxidase) appear unaffected by the disruption. Analysis of mitochondrial transcripts shows a reduced abundance of cytb mRNA, which in addition is approximately 200 nucleotides longer than that of the wild-type. Sequencing of the 3' region of the mutant cytb mRNA with an oligonucleotide primer positioned 148 nt downstream from the dodecamer sequence ("end-of-messenger" signal), demonstrates that the mutant transcript is extended beyond this position and is not processed at the conserved dodecamer cleavage site. The CBT1 gene product may be one of the components required for the exact 3' cleavage of the cytb messenger and may also be related to RNA splicing, since the intron-containing cytb gene is not as well expressed as the intron-less gene and the respiratory deficiency is more severe. We propose, that the CBT1 protein is necessary for the correct trimming of the end of cytb pre-mRNA and may be a part of the multi-component complex involved in this process.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Rieger KJ,Aljinovic G,Lazowska J,Pohl TM,Slonimski PP,Aljinovic Gdoi
10.1007/s002940050262subject
Has Abstractpub_date
1997-09-01 00:00:00pages
163-74issue
3eissn
0172-8083issn
1432-0983journal_volume
32pub_type
杂志文章相关文献
CURRENT GENETICS文献大全abstract::The amino acid sequences of two barley ribosomal proteins, termed HvL17-1 and HvL17-2, were decoded from green leaf cDNA clones. The N-terminal sequences of the derived barley proteins are 48% identical to the N-terminal amino acid sequence of protein YL17 from the large subunit of yeast cytoplasmic ribosomes. Via arc...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00309605
更新日期:1991-05-01 00:00:00
abstract::Botryotinia fuckeliana, the causal agent of grey mould, was biolistically transformed to hygromycin B resistance using a plasmid (pOHT) containing a bacterial hygromycin phosphotransferase gene fused to regulatory sequences from Aspergillus nidulans. Multiple copies of the plasmid, precipitated onto tungsten particles...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00309537
更新日期:1994-02-01 00:00:00
abstract::In this work, we explore the use of the unbiased cDNA-AFLP strategy to identify genes involved in Mn(2+) homeostasis in Ceriporiopsis subvermispora. In this ligninolytic white-rot fungus, whose genome has not yet been sequenced, three Mn peroxidase genes responding to Mn(2+) have been characterized. Using cDNA-AFLP to...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-008-0209-7
更新日期:2008-10-01 00:00:00
abstract::An Agrocybe aegerita cDNA library, constructed from fruit body primordia poly(A)+ RNAs, was screened by differential colony hybridization. Clones which preferentially hybridized to poly(A)+ RNA sequences from fruit body primordia, versus poly(A)+ RNAs from mycelium, were isolated. Eight of these clones (EMAa-1 to EMAa...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00326234
更新日期:1991-08-01 00:00:00
abstract::The recovery of spontaneous canavanine-resistant mutants is reduced dramatically in a strain of Saccharomyces cerevisiae that carries a suppressed can 1-100 allele and is permeable to and auxotrophic for thymidylate. This effect does not occur in an isogenic strain that neither takes up nor requires the nucleotide. Ho...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00435459
更新日期:1989-02-01 00:00:00
abstract::Cyh3 and cyh4, multiple drug resistant strains of Schizosaccharomyces pombe, show a much reduced uptake of trichodermin, chloramphenicol, cycloheximide, L-lysine, glycine, L-threonine, L-glutamine, L-arginine and L-glutamic acid when compared to wild type. The plasma membrane and mitochondrial ATPase activities of the...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00376075
更新日期:1983-07-01 00:00:00
abstract::The putative gene encoding acetyl-CoA carboxylase, accA, has been isolated from Aspergillus nidulans. This single-copy gene has an open reading frame (ORF) of 6864 bp and contains two small introns near the 5'-end. A short ORF upstream of the ATG start codon has been identified in this gene by RT-PCR. Based on sequenc...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050410
更新日期:1998-12-01 00:00:00
abstract::Using differential hybridization, a gene preferentially expressed during entry into stationary phase has been isolated. Subsequent analysis indicated that this gene corresponds to a heat-shock gene. The nucleotide sequence has been determined. It revealed a 332 amino-acid protein. No similarities to any previously kno...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00312631
更新日期:1993-05-01 00:00:00
abstract::Chromosomes of two closely related yeast strains, the amylolytic Saccharomyces diastaticus and the non-amylolytic Saccharomyces cerevisiae, were resolved by pulsed field gel electrophoresis (PFGE) and orthological field alteration gel electrophoresis (OFAGE). Electrophoretic karyotypes of these two strains are identic...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00405847
更新日期:1988-07-01 00:00:00
abstract::A screening of haploid yeast strains for enhanced resistance to nitrogen mustard (HN2) yielded a recessive mutant allele, hnm1, that conferred hyper-resistance (HYR) to HN2. Diploids, homo- or heterozygous for the HNM1 locus, exhibit normal wild-type like resistance while homozygosity for hnm1 leads to the phenotype H...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00318378
更新日期:1990-10-01 00:00:00
abstract::Adaptation by natural selection might improve the fitness of an organism and its probability to survive in unfavorable environmental conditions. Decoding the genetic basis of adaptive evolution is one of the great challenges to deal with. To this purpose, Saccharomyces cerevisiae has been largely investigated because ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-016-0635-x
更新日期:2017-05-01 00:00:00
abstract::How group I introns originate in nuclear ribosomal (r)RNA genes is an important question in evolutionary biology. Central to this issue is the multitude of group I introns present in evolutionarily distantly related plant, fungal, and protist lineages, together with an understanding of their origin and lateral transfe...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940100227
更新日期:2001-08-01 00:00:00
abstract::The Cdc42p GTPase regulates multiple signal transduction pathways through its interactions with downstream effectors. Specific functional domains within Cdc42p are required for guanine-nucleotide binding, interactions with downstream effectors, and membrane localization. However, little is known about how Cdc42p is cl...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-004-0505-9
更新日期:2004-06-01 00:00:00
abstract::A PCR approach, employing the use of degenerate oligonucleotide mixtures, was used to isolate pph-1, a type-2A protein phosphatase (catalytic subunit)-encoding gene, from Neurospora crassa. The isolated single copy gene is 1327 nucleotides in length, contains four putative introns and encodes a 310 amino-acid polypept...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00310816
更新日期:1995-10-01 00:00:00
abstract::Cell fusion occurs regularly during the vegetative and sexual phases of the life cycle in filamentous fungi. Here, we present a simple and efficient method that can detect even rare hyphal fusion events. Using the homothallic ascomycete Sordaria macrospora as an experimental system, we developed a histone-assisted mer...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-007-0158-6
更新日期:2007-11-01 00:00:00
abstract::The potato (Solanum tuberosum L.) mitochondrial cox3/ sdh4 gene cluster was previously identified by heterologous hybridization using a Marchantia polymorpha sdh4 probe. In this work, this potato gene cluster was cloned, sequenced and its expression was evaluated. The gene sequence and gene locus organization were fou...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-002-0294-y
更新日期:2002-07-01 00:00:00
abstract::Inorganic phosphate is an essential nutrient because it is required for the biosynthesis of nucleotides, phospholipids and metabolites in energy metabolism. During phosphate starvation, phosphatases play a major role in phosphate acquisition by hydrolyzing phosphorylated macromolecules. In Saccharomyces cerevisiae, PH...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-015-0544-4
更新日期:2016-05-01 00:00:00
abstract::The occupancy of nucleosomes governs access to the eukaryotic genomes and results from a combination of biophysical features and the effect of ATP-dependent remodelling complexes. Most promoter regions show a conserved pattern characterized by a nucleosome-depleted region (NDR) flanked by nucleosomal arrays. The conse...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-016-0642-y
更新日期:2017-05-01 00:00:00
abstract::From the first identification of bacteria as a causative agent of disease, researchers have been developing methods and techniques to understand their pathogenic processes. For decades, this work has been limited to looking at a small number of genetically manipulatable isolates in in vitro assays or animal models of ...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-016-0576-4
更新日期:2016-08-01 00:00:00
abstract::cAMP-dependent protein kinases (PKA) are ubiquitous signalling molecules that mediate many extracellular signals in eukaryotes from yeast to men. Directing PKA to its substrates is an important level of control to ensure specificity of cAMP-mediated signal transduction. Unlike in yeast and fungi, in mammalian cells a ...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-002-0308-9
更新日期:2002-07-01 00:00:00
abstract::Small insertions are reported from the SSU rDNA of the genera Cladonia, Cladina, Stereocaulon, Pertusaria and Physcia (lichen-forming Lecanorales, Ascomycetes). The insertions range in length from 56 to 81 nucleotides, and occur at a shared position, 330 (relative to Escherichia coli), in a semi-conserved region of th...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050317
更新日期:1998-02-01 00:00:00
abstract::In order to study pathogenic mechanisms of the plant pathogen Ascochyta rabiei, conditions for efficient transformation using Agrobacterium-mediated transformation were investigated. Hygromycin B resistance (hph) was superior to geneticin resistance (nptII) for selecting transformants, and the hph gene was more effici...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-005-0048-8
更新日期:2006-04-01 00:00:00
abstract::We have sequenced a region (7,376-bp) of the mitochondrial (mt) DNA (54 kb) of the cellular slime mold, Dictyostelium discoideum. From the DNA and amino-acid sequence comparisons with known sequences, genes for ATPase subunit 9 (ATP9), cytochrome b (CYTB), NADH dehydrogenase subunits 1, 3 and 6 (ND1, ND3 and ND6), sma...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00326157
更新日期:1995-02-01 00:00:00
abstract::The nucleotide sequences of regions containing the mating-type locus of the plant-pathogenic ascomycete Leptosphaeria maculans are described. The MAT1-1 gene is 1,368 bp, encoding a predicted protein of 441 amino acids, with a 45-bp intron. The MAT1-2 gene is 1,246 bp, encoding a predicted protein of 397 amino acids, ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-003-0391-6
更新日期:2003-08-01 00:00:00
abstract::The nucleotide sequence of the ERG12 gene, encoding mevalonate kinase, from Saccharomyces cerevisiae is presented. The longest open reading frame may code for a protein containing 443 amino acids with a deduced relative molecular mass of 48,500. The analysis of the nucleotide sequence reveals a complete identity with ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00362081
更新日期:1991-01-01 00:00:00
abstract::Ribosome is responsible for protein synthesis and is able to monitor the sequence and structure of the nascent peptide. Such ability plays an important role in determining overall gene expression profile of the bacteria through ribosome stalling and rescuing. In this review, we briefly summarize our current understand...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-015-0545-3
更新日期:2016-05-01 00:00:00
abstract::Schizosaccharomyces pombe Cdc23 is an essential DNA replication protein, conserved in eukaryotes and functionally homologous with Saccharomyces cerevisiae Dna43 (Mcm10). We sought evidence for interactions between Cdc23 and the MCM2-7 complex, a component of both the pre-replicative complex and the replication fork. C...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-002-0316-9
更新日期:2002-08-01 00:00:00
abstract::The cellulase gene cel3 has been isolated from Agaricus bisporus and sequenced. The 5'-end of the cel3 transcript was determined by primer extension and S1 nuclease protection. Putative regulatory elements have been identified in the cel3 promoter and 3'-untranslated regions. The cel3 coding region is interrupted by s...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050100
更新日期:1996-06-01 00:00:00
abstract::Extraction of RNA from Tetrahymena mitochondrial ribosomes yields several RNA species, including a "large" 21S molecule, a "small" 14S molecule, a 7S molecule, and other smaller RNAs. The molecular weight of the 14S rRNA indicates that it is about 1,300 bases in length. We have sequenced the 14S rRNA gene and, by alig...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00384616
更新日期:1987-01-01 00:00:00
abstract::Mitochondrial (mt) DNA structures were studied in 12 nucleus-cytoplasm (NC) hybrids of tetraploid and hexaploid wheat in which nuclear genomes of the recurrent paternal wheat parents were combined with D and D2 plasmons from Aegilops species. RFLP analysis of 15 mtDNA regions indicated the presence of the paternal seq...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940000153
更新日期:2000-11-01 00:00:00