Multiple drug resistance in the fission yeast Schizosaccharomyces pombe: Correlation between drug and amino acid uptake and membrane ATPase activities.

abstract：:A system for the expression of an ATP binding cassette (ABC) transporter from the soybean pathogen Phytophthora sojae is described. Pdr1, an ABC transporter with homology to the pleiotropic drug resistance (PDR) family of transporters, was cloned by primer walking from a P. sojae genomic library. Reverse transcriptase...

journal_title：Current genetics

authors： Connolly MS,Sakihama Y,Phuntumart V,Jiang Y,Warren F,Mourant L,Morris PF

更新日期：2005-12-01 00:00:00

abstract：:In order to study the mechanism responsible for the uniparental transmission of the mitochondrial genome in crosses between Chlamydomonas reinhardtii and C. smithii, we have analyzed the fate of mitochondrial DNA during gametogenesis, zygospore differentiation and sporulation by hybridization experiments. Both mt+ and...

journal_title：Current genetics

authors： Beckers MC,Munaut C,Minet A,Matagne RF

更新日期：1991-08-01 00:00:00

abstract：:From the first identification of bacteria as a causative agent of disease, researchers have been developing methods and techniques to understand their pathogenic processes. For decades, this work has been limited to looking at a small number of genetically manipulatable isolates in in vitro assays or animal models of ...

journal_title：Current genetics

authors： Laabei M,Massey R

更新日期：2016-08-01 00:00:00

abstract：:Phyto-pathogenic fungi can cause huge damage to crop production. During millions of years of coexistence, fungi have evolved diverse life-style to obtain nutrients from the host and to colonize upon them. They deploy various proteinaceous as well as non-proteinaceous secreted molecules commonly referred as effectors t...

journal_title：Current genetics

authors： Pradhan A,Ghosh S,Sahoo D,Jha G

更新日期：2020-11-04 00:00:00

abstract：:DNA polymerases are evolved to extend the 3'-OH of a growing primer annealed to a template DNA substrate. Since replicative DNA polymerases have a limited role while replicating structurally distorted template, translesion DNA polymerases mostly from Y-family come to the rescue of stalled replication fork and maintain...

journal_title：Current genetics

authors： Acharya N,Manohar K,Peroumal D,Khandagale P,Patel SK,Sahu SR,Kumari P

更新日期：2019-06-01 00:00:00

abstract：:In this paper we report the cloning, sequencing and functional characterization of CEN12 and an associated autonomously replicating sequence (ARS) from the budding yeast Saccharomyces cerevisiae. In the course of studying a dynamin-related gene, DNM1, we previously physically mapped the gene to chromosome 12. Genetic ...

journal_title：Current genetics

authors： Gammie AE,Rose MD

更新日期：1995-11-01 00:00:00

abstract：:Chloroplast DNA from the fern Osmunda einnamomea was isolated by a sucrose gradient procedure utilizing PEG to stabilize chloroplasts. Analysis with the restriction endonucleases PvuII, Sacl and BstEII indicates a chloroplast genome size of 144 kb. A physical map of the fragments produced by these three enzymes was co...

journal_title：Current genetics

authors： Palmer JD,Stein DB

更新日期：1982-08-01 00:00:00

abstract：:An exceptional mode of mtDNA inheritance involving separate maternal and paternal transmission routes has been reported recently in the mussel Mytilus edulis. This mode of inheritance provides an explanation for the high levels of heteroplasmy for two highly diverged genomes observed in males of this species. Here we ...

journal_title：Current genetics

authors： Quesada H,Skibinski DA,Skibinski DO

更新日期：1996-04-01 00:00:00

abstract：:Selection of mutants of Saccharomyces cerevisiae sensitive to the DNA cross-linking agent nitrogen mustard (HN2) at two temperatures (23 °C and 36 °C) yielded two isolates with thermoconditionally enhanced (ts) sensitivity to the mutagen. Both were due to single recessive nuclear genes. Mutant allele snm1-2 (ts) showe...

journal_title：Current genetics

authors： Siede W,Brendel M

更新日期：1981-11-01 00:00:00

abstract：:We have isolated suppressors of a PHO4c (a positive regulator) mutant which normally confers weak constitutivity for acid phosphatase production on the Saccharomyces cell. One dominant suppressor (PHO80-2) was found to be an allele of PHO80 (a negative regulator) that changes G to A, resulting in substitution of isole...

journal_title：Current genetics

authors： Okada H,Toh-e A

更新日期：1992-02-01 00:00:00

abstract：:Most cheese-making filamentous fungi lack suitable molecular tools to improve their biotechnology potential. Penicillium roqueforti, a species of high industrial importance, would benefit from functional data yielded by molecular genetic approaches. This work provides the first example of gene replacement by homologou...

journal_title：Current genetics

authors： Goarin A,Silar P,Malagnac F

更新日期：2015-05-01 00:00:00

abstract：:The gene for subunit II of cytochrome oxidase in the yeast Hansenula saturnus was previously shown to be located on a 1.7 kb HindIII-BamHI fragment of mitochondrial DNA (Lawson and Deters, accompanying paper). In this paper, we report the nucleotide sequence of a large part of this fragment, covering the coding region...

journal_title：Current genetics

authors： Lawson JE,Deters DW

更新日期：1985-01-01 00:00:00

abstract：:Similarity searches with Didymium iridis mitochondrial genomic DNA identified six possible ribosomal protein-coding regions, however, each region contained stop codons that would need to be removed by RNA editing to produce functional transcripts. RT-PCR was used to amplify these regions from total RNA for cloning and...

journal_title：Current genetics

authors： Hendrickson PG,Silliker ME

更新日期：2010-06-01 00:00:00

abstract：:A simple method is described for demonstrating adaptation to alkylation damage in Aspergillus nidulans. One wild type, two MNNG-sensitive, and one MNNG-resistant strain all showed improvement in colony growth when challenged with MNNG following appropriate inducing pretreatments. Other alkylating agents (MMS, EMS) cou...

journal_title：Current genetics

authors： Hooley P,Shawcross SG,Strike P

更新日期：1988-11-01 00:00:00

abstract：:Cohesin is essential for sister chromatid cohesion, which ensures equal segregation of the chromatids to daughter cells. However, the molecular mechanism by which cohesin mediates this function is elusive. Scc3, one of the four core subunits of cohesin, is vital to cohesin activity. However, the mechanism by which Scc...

journal_title：Current genetics

authors： Pathania A,Liu W,Matityahu A,Irudayaraj J,Onn I

更新日期：2021-01-06 00:00:00

abstract：:Mixed aeciospore isolates of Cronartium flaccidum and Peridermium pini were obtained from single-tree infections in Britain, Italy and Greece. The 5.8s ribosomal RNA gene and flanking intergenic transcribed spacer regions ITS1 and ITS2 were found to be highly similar between C. flaccidum and P. pini. Within samples he...

journal_title：Current genetics

authors： Moricca S,Kasuga T,Mitchelson K,Ragazzi A,Diamandis S

更新日期：1996-03-01 00:00:00

abstract：:A Neurospora crassa cytoplasmic ribosomal protein gene, crp-5, has been isolated and characterized. The cDNA was isolated by a differential screening of a cDNA library for glucose-inducible genes. The cDNA was subsequently used to identify and isolate crp-5 genomic sequences. Computer analysis of the DNA sequences sho...

journal_title：Current genetics

authors： Wang Z,Tarawneh KA,Free SJ

更新日期：1993-01-01 00:00:00

abstract：:We recently discovered the novel non-chromosomal determinant in Saccharomyces cerevisiae [NSI(+)] (nonsense suppression inducer), which causes omnipotent nonsense suppression in strains where the Sup35 N-terminal domain is deleted. [NSI(+)] possesses yeast prion features and does not correspond to previously identifie...

journal_title：Current genetics

authors： Nizhnikov AA,Magomedova ZM,Rubel AA,Kondrashkina AM,Inge-Vechtomov SG,Galkin AP

更新日期：2012-02-01 00:00:00

abstract：:Cell aging is the result of deteriorating competence in maintaining cellular homeostasis and quality control. Certain cell types are able to rejuvenate through asymmetric cell division by excluding aging factors, including damaged cellular compartments and extrachromosomal rDNA circles, from entering the daughter cell...

journal_title：Current genetics

authors： Koch-Bojalad BA,Carson L,Yu HG

更新日期：2020-11-27 00:00:00

abstract：:Mitotic homologous recombination (HR) is an important mechanism for the repair of double-strand breakS and errors occurring during DNA replication. It is likely that the recombinational repair of DNA lesions occurs preferentially by sister chromatid exchanges that have no genetic consequences. However, most geneticall...

journal_title：Current genetics

authors： Prado F,Cortés-Ledesma F,Huertas P,Aguilera A

更新日期：2003-01-01 00:00:00

abstract：:Microbial pathogens secrete an array of cell wall-degrading enzymes to break down the structure of the host cell wall to facilitate colonization of the host tissue. To better understand their role in the pathogenesis, a putative endoglucanase from Magnaporthe grisea was characterized in this paper. SignalP-3.0 analysi...

journal_title：Current genetics

authors： Zhou J,Zheng XZ,Lan L,Lin CZ,Wu YB,Lin XJ,Ebbole D,Lu GD,Wang ZH

更新日期：2008-04-01 00:00:00

abstract：:The cytoplasmic virus-like element pWR1A from Debaryomyces robertsiae encodes a toxin (DrT) with similarities to the Pichia acaciae killer toxin PaT, which acts by importing a toxin subunit (PaOrf2) with tRNA anticodon nuclease activity into target cells. As for PaT, loss of the tRNA methyltransferase Trm9 or overexpr...

journal_title：Current genetics

authors： Klassen R,Kast A,Wünsche G,Paluszynski JP,Wemhoff S,Meinhardt F

更新日期：2014-08-01 00:00:00

abstract：:A fine restriction map of the linear mitochondrial DNA of Tetrahymena pyriformis strain ST is presented. 1. Based on agarose gel electrophoresis data together with limited nucleotide sequences available on some restriction fragments, we estimate the actual size of this genome to be about 55,000 base pairs. 2. Seven tR...

journal_title：Current genetics

authors： Suyama Y,Fukuhara H,Sor F

更新日期：1985-01-01 00:00:00

abstract：:A PCR-based technique, involving the random amplification of polymorphic DNA (RAPD), was used for assessing genomic variability among a wide range of culture collection strains of black Aspergilli and related species. The performance of this technique is compared with that of the two other genetic techniques most comm...

journal_title：Current genetics

authors： Megnegneau B,Debets F,Hoekstra RF

更新日期：1993-01-01 00:00:00

abstract：:Sister chromatid cohesion is essential for chromosome segregation both in mitosis and meiosis. Cohesion between two chromatids is mediated by a protein complex called cohesin. The loading and unloading of the cohesin are tightly regulated during the cell cycle. In vertebrate cells, cohesin is released from chromosomes...

journal_title：Current genetics

authors： Challa K,Shinohara M,Shinohara A

更新日期：2019-08-01 00:00:00

abstract：:A vector was constructed for transformation of the plant pathogenic fungus Fusarium solani. The promoter 35Sp, from cauliflower mosaic virus, was fused to the bacterial gene APH(3')II, which confers resistance to the aminoglycoside antibiotic G418. Two transformation procedures were developed: one using isolated funga...

journal_title：Current genetics

authors： Marek ET,Schardl CL,Smith DA

更新日期：1989-06-01 00:00:00

abstract：:Two different cytoplasmic male-sterility (CMS) systems, nap and pol, are found in the oilseed rape (canola) species Brassica napus. Physical mapping studies have previously shown that organizational differences between the sterile pol and fertile cam mitochondrial genomes are restricted to a relatively small region im...

journal_title：Current genetics

authors： L'Homme Y,Stahl RJ,Li XQ,Hameed A,Brown GG

更新日期：1997-04-01 00:00:00

abstract：:In this work, we explore the use of the unbiased cDNA-AFLP strategy to identify genes involved in Mn(2+) homeostasis in Ceriporiopsis subvermispora. In this ligninolytic white-rot fungus, whose genome has not yet been sequenced, three Mn peroxidase genes responding to Mn(2+) have been characterized. Using cDNA-AFLP to...

journal_title：Current genetics

authors： Gutiérrez M,Rojas LA,Mancilla-Villalobos R,Seelenfreund D,Vicuña R,Lobos S

更新日期：2008-10-01 00:00:00

abstract：:By completing and correcting the sequence of a 1.8 kb DNA segment downstream of the oxi2 gene of Saccharomyces cerevisiae, a long, potentially coding sequence ("RF2") has been identified. The sequence is rather closely related to the RF1 open reading frame, downstream of the oxil gene, and, further, to the major famil...

journal_title：Current genetics

authors： Michel F

更新日期：1984-05-01 00:00:00

abstract：:ERG24 is the structural gene for the C14-sterol reductase in yeast. A lack of activity in that enzyme, mediated either by the morpholine fungicides or the insertional inactivation of ERG24, causes the accumulation of the aberrant sterol ignosterol. Cells producing this sterol are unable to grow aerobically in the rout...

journal_title：Current genetics

authors： Crowley JH,Tove S,Parks LW

更新日期：1998-08-01 00:00:00