cDNA encoding protein O-mannosyltransferase from the filamentous fungus Trichoderma reesei; functional equivalence to Saccharomyces cerevisiae PMT2.

abstract：:Nucleic acid extracted from killer strains of Saccharomyces cerevisiae and run on agarose gels under sub-optimal conditions for M dsRNA yielded two species of L dsRNA (L1 and L2) which migrated at slightly different rates. L2 dsRNA was shown to be dependent upon MAK3, MAK10 and PES1 but not MAK1 nuclear genes for its ...

journal_title：Current genetics

authors： El-Sherbeini M,Bevan EA,Mitchell DJ

更新日期：1983-03-01 00:00:00

abstract：:A screening of haploid yeast strains for enhanced resistance to nitrogen mustard (HN2) yielded a recessive mutant allele, hnm1, that conferred hyper-resistance (HYR) to HN2. Diploids, homo- or heterozygous for the HNM1 locus, exhibit normal wild-type like resistance while homozygosity for hnm1 leads to the phenotype H...

journal_title：Current genetics

authors： Haase E,Brendel M

更新日期：1990-10-01 00:00:00

abstract：:Loss of heterozygosity (LOH) in a vegetatively growing diploid cell signals irregularity of mitosis. Therefore, assays of LOH serve to discover pathways critical for proper replication and segregation of chromosomes. We screened for enhanced LOH in a whole-genome collection of diploid yeast strains in which a single g...

journal_title：Current genetics

authors： Tutaj H,Pogoda E,Tomala K,Korona R

更新日期：2019-04-01 00:00:00

abstract：:In order to analyse gene expression during fruit body development of the ectomychorrizal fungus Tuber borchii Vittad., a modified differential display procedure was set up. The procedure used is easier and faster than the traditional one and generates reproducible cDNA banding patterns that can be resolved on a standa...

journal_title：Current genetics

authors： Zeppa S,Guidi C,Zambonelli A,Potenza L,Vallorani L,Pierleoni R,Sacconi C,Stocchi V

更新日期：2002-12-01 00:00:00

abstract：:The Saccharomyces cerevisiae nuclear gene MRP-L6 was cloned by complementation of the respiratory-deficient mutant pet-ts 2523 with a library of wild-type yeast genomic DNA. The isolated gene was part of a 3.8-kb sequenced DNA fragment containing, in addition to MRP-L6, two unassigned reading frames, ORF1 and ORF2. MR...

journal_title：Current genetics

authors： Harrer R,Schwank S,Schüller HJ,Schweizer E

更新日期：1993-07-01 00:00:00

abstract：:The core enzyme of mitochondrial RNA polymerase in yeast is homologous to those of bacteriophages T3, T7 and SP6. In previous studies the identification of the first conditional yeast mutant for this enzyme helped to identify the corresponding specificity factor and to elucidate their interaction inside mitochondria. ...

journal_title：Current genetics

authors： Lisowsky T,Stein T,Michaelis G,Guan MX,Chen XJ,Clark-Walker GD

更新日期：1996-11-01 00:00:00

abstract：:A procedure for stable transformation was developed for Phytophthora megasperma f. sp. glycinea, an oomycete pathogen of soybean. Transformants were obtained using a bacterial hygromycin resistance gene fused to a promoter and terminator from the ham34 gene of another oomycete, Bremia lactucae. Vector DNA, alone or co...

journal_title：Current genetics

authors： Judelson HS,Coffey MD,Arredondo FR,Tyler BM

更新日期：1993-03-01 00:00:00

abstract：:DNA analysis of Cephalosporium acremonium revealed that this important fungus for biotechnology contains, apart from chromosomal DNA, two types of extrachromosomal DNA which may be useful for the development of a homologous vector system: 1. Plasmid-like (pl) DNA consisting of circular molecules, but of rather heterog...

journal_title：Current genetics

authors： Minut W,Tudzynsk P,Esser K

更新日期：1982-08-01 00:00:00

abstract：:The cellulase gene cel3 has been isolated from Agaricus bisporus and sequenced. The 5'-end of the cel3 transcript was determined by primer extension and S1 nuclease protection. Putative regulatory elements have been identified in the cel3 promoter and 3'-untranslated regions. The cel3 coding region is interrupted by s...

journal_title：Current genetics

authors： Yagüe E,Chow CM,Challen MP,Thurston CF

更新日期：1996-06-01 00:00:00

abstract：:The cytoplasmic virus-like element pWR1A from Debaryomyces robertsiae encodes a toxin (DrT) with similarities to the Pichia acaciae killer toxin PaT, which acts by importing a toxin subunit (PaOrf2) with tRNA anticodon nuclease activity into target cells. As for PaT, loss of the tRNA methyltransferase Trm9 or overexpr...

journal_title：Current genetics

authors： Klassen R,Kast A,Wünsche G,Paluszynski JP,Wemhoff S,Meinhardt F

更新日期：2014-08-01 00:00:00

abstract：:A fine restriction map of the linear mitochondrial DNA of Tetrahymena pyriformis strain ST is presented. 1. Based on agarose gel electrophoresis data together with limited nucleotide sequences available on some restriction fragments, we estimate the actual size of this genome to be about 55,000 base pairs. 2. Seven tR...

journal_title：Current genetics

authors： Suyama Y,Fukuhara H,Sor F

更新日期：1985-01-01 00:00:00

abstract：:Cyclosporin A is a potent and clinically-important immunosuppressive drug (SandimmunR). It is produced by the fungus Tolypocladium niveum. A transformation system for T. niveum ATCC34921 based on hygromycin selection was established. In order to obtain a T. niveum promoter, the cyclophilin gene was isolated using the ...

journal_title：Current genetics

authors： Weber G,Leitner E

更新日期：1994-11-01 00:00:00

abstract：:To gain a deeper insight into the evolution of mating-type genes from filamentous ascomycetes, a comprehensive sequence analysis of PCR-amplified sequences corresponding to A- and a-specific mating-type sequences was undertaken. The study included nine homothallic (compatible) and eight heterothallic (incompatible) me...

journal_title：Current genetics

authors： Pöggeler S

更新日期：1999-10-01 00:00:00

abstract：:Cellulases belong to the large family of glycosyl hydrolases (GHs) and are produced by a variety of bacteria and fungi. These extracellular enzymes act as endoglucanases (EGs), cellobiohydrolases or beta-glucosidases. In this paper, we describe molecular screening for EGs from the GH family 12. Using three homologous ...

journal_title：Current genetics

authors： Goedegebuur F,Fowler T,Phillips J,van der Kley P,van Solingen P,Dankmeyer L,Power SD

更新日期：2002-05-01 00:00:00

abstract：:A molecular clone containing the complete sequence of a mitochondrial circular plasmid-like DNA (the R plasmid) isolated from the date-palm variety V3DP was used as a probe in Southern analyses of mitochondrial DNA prepared from other varieties. Another circular structure (the S plasmid) was detected in some of these ...

journal_title：Current genetics

authors： Benslimane AA,Hartmann C,Ouenzar B,Rode A

更新日期：1996-05-01 00:00:00

abstract：:The Hansenula polymorpha per6-210 mutant is impaired in respect of growth on methanol (Mut-) and is characterized by aberrant peroxisome formation. The functionally complementing DNA fragment contains two open reading frames. The first encodes dihydroxyacetone kinase (DAK), a cytosolic enzyme essential for formaldehyd...

journal_title：Current genetics

authors： van der Klei IJ,van der Heide M,Baerends RJ,Rechinger KB,Nicolay K,Kiel JA,Veenhuis M

更新日期：1998-07-01 00:00:00

abstract：:DNA replication checkpoint is a cell signaling pathway that is activated in response to perturbed replication. Although it is crucial for maintaining genomic integrity and cell survival, the exact mechanism of the checkpoint signaling remains to be understood. Emerging evidence has shown that RecQ helicases, a large f...

journal_title：Current genetics

authors： Ahamad N,Khan S,Mahdi ATA,Xu YJ

更新日期：2021-01-11 00:00:00

abstract：:Two plasmids containing Saccharomyces cerevisiae 2 µm DNA sequences and the S. cerevisiae LEU2 gene have been found to display incompatibility with 2 µm DNA; in the presence of the LEU2 plasmids, 2 µm DNA can be lost. The LEU2 plasmids can be lost spontaneously after (and before) 2 µm DNA loss has occurred, so that st...

journal_title：Current genetics

authors： Dobson MJ,Futcher AB,Cox BS

更新日期：1980-12-01 00:00:00

abstract：:We have determined the nucleotide sequence of a region of the mitochondrial genome of the yeast Saccharomyces douglasii which contains the ATPase subunit 9 gene and part of the intergenic sequences that surround it. The gene is 228 nucleotides long and encodes a polypeptide of 76 aa. A comparison of the coding sequenc...

journal_title：Current genetics

authors： Nicoletti L,Laveder P,Pellizzari R,Cardazzo B,Carignani G

更新日期：1994-06-01 00:00:00

abstract：:Individual perithecia from selfings of most Ceratocystis species produce both self-fertile and self-sterile progeny, apparently due to uni-directional mating-type switching. In C. coerulescens, male-only mutants of otherwise hermaphroditic and self-fertile strains were self-sterile and were used in crossings to demons...

journal_title：Current genetics

authors： Harrington TC,McNew DL

更新日期：1997-07-01 00:00:00

abstract：:We have further characterized the functionality of the Saccharomyces cerevisiae gene SLT2(MPK1), coding for a MAP-kinase homolog essential for cell integrity, which is involved in the Pkc1p signalling pathway. This gene was isolated on the basis of its capacity to complement the thermosensitive-autolytic, osmotic-reme...

journal_title：Current genetics

authors： Martín H,Castellanos MC,Cenamor R,Sánchez M,Molina M,Nombela C

更新日期：1996-05-01 00:00:00

abstract：:The nature of extra-chromosomal maintenance of the transforming plasmid p12-6 in Phanerochaete chrysosporium was studied. Our results indicate that the plasmid is maintained in the fungal transformants extra-chromosomally as part of a larger endogenous plasmid (designated pME) of P. chrysosporium. Using the total DNA ...

journal_title：Current genetics

authors： Randall TA,Reddy CA

更新日期：1992-03-01 00:00:00

abstract：:Primary transcripts in higher plant cell organelles undergo a series of essential RNA processing steps, including 5'- and 3'-terminal maturation, intron splicing and RNA editing. Most of the protein factors involved in these RNA processing mechanisms are still unknown. Also, little is known about the environmental and...

journal_title：Current genetics

authors： Karcher D,Bock R

更新日期：2002-04-01 00:00:00

abstract：:A cDNA library made from Euglena polyadenylated RNA has been constructed in the expression vector lambda gt11. The library was screened with antibodies directed against the LHCP apoproteins. Several putative LHCP clones were isolated and analyzed. We present the nucleotide and derived amino-acid sequence of an 800 bp ...

journal_title：Current genetics

authors： Houlne G,Schantz R

更新日期：1987-01-01 00:00:00

abstract：:RNA/DNA hybrids are processed by RNases H1 and H2, while single ribonucleoside-monophosphates (rNMPs) embedded in genomic DNA are removed by the error-free, RNase H2-dependent ribonucleotide excision repair (RER) pathway. In the absence of RER, however, topoisomerase 1 (Top1) can cleave single genomic rNMPs in a mutag...

journal_title：Current genetics

authors： Cerritelli SM,El Hage A

更新日期：2020-12-01 00:00:00

abstract：:The hybrid vector pCP2, consisting of the bacterial plasmid pBR325, the nuclear gene Leu-2 of Saccharomyces cerevisiae and a fragment of mitochondrial DNA from Cephalosporium acremonium, was found to associate with the nucleus in a transformed strain of Saccharomyces cerevisiae. This was inducted by (1) efficient expr...

journal_title：Current genetics

authors： Tudzynski P,Esser K

更新日期：1983-04-01 00:00:00

abstract：:We find three interesting characteristics of the genes encoding the ribosomal RNA (rRNA) in the basidiomycete Coprinus cinereus. First, there are only 60 to 90 copies of the genes, fewer than in other fungi. Second, the genes are organized in an unusual arrangement. The 5S rRNA genes are located in the repeat unit whi...

journal_title：Current genetics

authors： Rhodes Cassidy J,Moore D,Lu BC,Pukkila PJ

更新日期：1984-10-01 00:00:00

abstract：:By completing and correcting the sequence of a 1.8 kb DNA segment downstream of the oxi2 gene of Saccharomyces cerevisiae, a long, potentially coding sequence ("RF2") has been identified. The sequence is rather closely related to the RF1 open reading frame, downstream of the oxil gene, and, further, to the major famil...

journal_title：Current genetics

authors： Michel F

更新日期：1984-05-01 00:00:00

abstract：:Glutathione (GSH) is an abundantly produced low-molecular-weight (LMW) thiol in many organisms. However, a number of Gram-positive bacteria do not produce GSH, but instead produce bacillithiol (BSH) as one of the major LMW thiols. Similar to GSH, studies have found that BSH has various roles in the cell, including pro...

journal_title：Current genetics

authors： Rosario-Cruz Z,Boyd JM

更新日期：2016-02-01 00:00:00

abstract：:Mutations in the RecA-like genes RAD51 and RAD57 reduce the frequency of gene conversion/reciprocal exchange between inverted repeats 7-fold. However, they enhance the frequency of deletions between direct repeats 5-12-fold. These induced deletions are RAD1- and RAD52-dependent. On the basis of these results it is pro...

journal_title：Current genetics

authors： Aguilera A

更新日期：1995-03-01 00:00:00