Intramolecular recombination of a mitochondrial minicircular plasmid-like DNA of date-palm mediated by a set of short direct-repeat sequences.

abstract：:Neurospora crassa wild type genome shows DNA sequences which are homologous to the sequences present in the rRNA processing genes of the yeast Saccharomyces cerevisiae. Five such processing genes from yeast, viz., RNA1 through RNA5, cloned in plasmid pBR322 were transformed in Escherichia coli strain LE392. Southern b...

journal_title：Current genetics

authors： Dutta SK,Verma M,Verma M

更新日期：1987-01-01 00:00:00

abstract：:Phyto-pathogenic fungi can cause huge damage to crop production. During millions of years of coexistence, fungi have evolved diverse life-style to obtain nutrients from the host and to colonize upon them. They deploy various proteinaceous as well as non-proteinaceous secreted molecules commonly referred as effectors t...

journal_title：Current genetics

authors： Pradhan A,Ghosh S,Sahoo D,Jha G

更新日期：2020-11-04 00:00:00

abstract：:Uridine diphosphoglucose is not the sole donor for trehalose synthesis in yeast cells: an ADPG-dependent trehalose synthase, has been identified in mutant strains with undetectable UDPG-dependent trehalose-6-P synthase activity. Genetic and chromatographic studies indicate that the two activities correspond to differe...

journal_title：Current genetics

authors： Paschoalin VM,Silva JT,Panek AD

更新日期：1989-08-01 00:00:00

abstract：:Cells of all living organisms contain complex signal transduction networks to ensure that a wide range of physiological properties are properly adapted to the environmental conditions. The fundamental concepts and individual building blocks of these signalling networks are generally well-conserved from yeast to man; y...

journal_title：Current genetics

authors： Smets B,Ghillebert R,De Snijder P,Binda M,Swinnen E,De Virgilio C,Winderickx J

更新日期：2010-02-01 00:00:00

abstract：:In order to study resistance to DNA damaging agents, yeast DNA segments conferring hyperresistance in this organism to such genotoxic agents were selected for among yeast cells transformed by a yeast genome library based on the multi-copy vector plasmid YEp13. Genetic variants hyperresistant to 4-nitroquinoline-N-oxid...

journal_title：Current genetics

authors： Ruhland A,Brendel M,Haynes RH

更新日期：1986-01-01 00:00:00

abstract：:The mother-bud neck is defined as the boundary between the mother cell and bud in budding microorganisms, wherein sequential morphological events occur throughout the cell cycle. This study was designed to quantitatively investigate the morphology of the mother-bud neck in budding yeast Saccharomyces cerevisiae. Obser...

journal_title：Current genetics

authors： Kubo K,Okada H,Shimamoto T,Kimori Y,Mizunuma M,Bi E,Ohnuki S,Ohya Y

更新日期：2019-02-01 00:00:00

abstract：:Ziziphus jujuba is an important woody plant with high economic and medicinal value. Here, we analyzed and characterized the complete chloroplast (cp) genome of Z. jujuba, the first member of the Rhamnaceae family for which the chloroplast genome sequence has been reported. We also built a web browser for navigating th...

journal_title：Current genetics

authors： Ma Q,Li S,Bi C,Hao Z,Sun C,Ye N

更新日期：2017-02-01 00:00:00

abstract：:Mitochondrial DNA from etiolated seedling of male-fertile (F) and male-sterile (S) sunflower (Helianthus annuus L.) has been investigated for the first time in an isogenic couple HA89. 1. The mt-DNAs isolated from the S and F cytoplasms give a sharp and symmetrical band at 1.706 g/ml in an analytical CsCI gradient ult...

journal_title：Current genetics

authors： Leroy P,Bazetoux S,Quétier F,Delbut J,Bervillé A

更新日期：1985-03-01 00:00:00

abstract：:The generation of a comprehensive EST library and the sequencing of its genome set the stage for reverse genetics approaches in Chlamydomonas reinhardtii. However, these also require tools for the specific downregulation of target gene expression. Consequently, a large number of diverse constructs were developed aimed...

journal_title：Current genetics

authors： Schroda M

更新日期：2006-02-01 00:00:00

abstract：:A phylogenetic investigation of the autosporic chlorophycean alga species Selenastrum capricornutum using the small subunit (SSU) rRNA gene revealed the unusual presence of six group IC1 introns. Previous studies showed that numerous green algal taxa contain group IC1 introns. However, whereas some algal species harbo...

journal_title：Current genetics

authors： Booton GC,Floyd GL,Fuerst PA

更新日期：2004-10-01 00:00:00

abstract：:Bands of dsRNA were detected in five out of seven isolates of the entomopathogenic fungus Metarhizium flavoviride. The identity of these bands was proven by RNase and S1-nuclease treatments. The transference of dsRNA between isolates (from CG291 to CG442) was successfully carried out through forced heterokaryons. Isog...

journal_title：Current genetics

authors： Martins MK,Furlaneto MC,Sosa-Gomez DR,Faria MR,Fungaro MH

更新日期：1999-08-01 00:00:00

abstract：:Genetic analysis of the MAL6 locus has previously yielded mal6 mutants which fall into a single complementation group and which are noninducible for maltase and maltose permease. However, the strains used in these studies contained additional partially functional copies of MAL1 (referred to as MAL1g) and MAL3 (referre...

journal_title：Current genetics

authors： Chang YS,Dubin RA,Perkins E,Forrest D,Michels CA,Needleman RB

更新日期：1988-09-01 00:00:00

abstract：:In this work, we explore the use of the unbiased cDNA-AFLP strategy to identify genes involved in Mn(2+) homeostasis in Ceriporiopsis subvermispora. In this ligninolytic white-rot fungus, whose genome has not yet been sequenced, three Mn peroxidase genes responding to Mn(2+) have been characterized. Using cDNA-AFLP to...

journal_title：Current genetics

authors： Gutiérrez M,Rojas LA,Mancilla-Villalobos R,Seelenfreund D,Vicuña R,Lobos S

更新日期：2008-10-01 00:00:00

abstract：:Nucleic acid extracted from killer strains of Saccharomyces cerevisiae and run on agarose gels under sub-optimal conditions for M dsRNA yielded two species of L dsRNA (L1 and L2) which migrated at slightly different rates. L2 dsRNA was shown to be dependent upon MAK3, MAK10 and PES1 but not MAK1 nuclear genes for its ...

journal_title：Current genetics

authors： El-Sherbeini M,Bevan EA,Mitchell DJ

更新日期：1983-03-01 00:00:00

abstract：:In eukaryotes, genomic DNA is packaged into the nucleus together with histone proteins, forming chromatin. The fundamental repeating unit of chromatin is the nucleosome, a naturally symmetric structure that wraps DNA and is the substrate for numerous regulatory post-translational modifications. However, the biological...

journal_title：Current genetics

authors： Ichikawa Y,Kaufman PD

更新日期：2019-04-01 00:00:00

abstract：:Saccharomyces cerevisiae adapts to oxidative, osmotic stress and nutrient deprivation through transcriptional changes, decreased proliferation, and entry into other developmental pathways such as pseudohyphal formation and sporulation. Inositol pyrophosphates are necessary for these cellular responses. Inositol pyroph...

journal_title：Current genetics

authors： Morrissette VA,Rolfes RJ

更新日期：2020-10-01 00:00:00

abstract：:A strain dependent growth on lactate in the presence of antimycin A (AA) has been observed - the strain D261 can grow on lactate and AA, whereas in the strain K8/6C antimycin A prevents the utilization of lactate and the induction of LDH.Genetic analysis demonstrates that growth on lactate in the presence of AA segreg...

journal_title：Current genetics

authors： Ferrero I,Rambaldelli R,Genga AM,Donnini C,Puglisi PP

更新日期：1984-08-01 00:00:00

abstract：:In Chlamydomonas reinhardtii, one displacement loop region which initiates the replication of chloroplast DNA was located on a 1.05 kb restriction fragment. This fragment was cloned and sequenced. In this report, the galK expression plasmid, pKO1 was used to screen for the presence of any prokaryotic promoter within t...

journal_title：Current genetics

authors： Wu M,Kong XF,Kung SD

更新日期：1986-01-01 00:00:00

abstract：:The mitochondrial (mt) genome of the sea anemone Metridium senile contains genes for only two transfer RNAs (tRNAs), tRNAf-Met and tRNATrp. Experiments were conducted to seek evidence for the occurrence of functional tRNAs corresponding to these genes and for the participation of nuclear DNA-encoded tRNAs in mt-protei...

journal_title：Current genetics

authors： Beagley CT,Wolstenholme DR

更新日期：2013-08-01 00:00:00

abstract：:We report the isolation and expression profiles of a single-copy gene from the mycoparasitic fungus Trichoderma hamatum encoding a 60 S cytoplasmic ribosomal protein. The gene, named RPL36, was cloned through its nutrient-mediated expression, using mRNA differential screening. Its predicted ORF, interrupted by two int...

journal_title：Current genetics

authors： Fekete C,Posta K,Hornok L

更新日期：2001-05-01 00:00:00

abstract：:Wild-type Neurospora crassa, strain Singapore, was transformed with a N. crassa metallothionein promoter/protyrosinase fusion gene. Transformants produced tyrosinase during vegetative growth, as determined by Western analyses and activity assays. This is in sharp contrast to wild-type strains, where this enzyme is onl...

journal_title：Current genetics

authors： Kupper U,Linden M,Cao KZ,Lerch K

更新日期：1990-11-01 00:00:00

abstract：:The Saccharomyces cerevisiae nuclear gene MRP-S9 was identified as part of the European effort in sequencing chromosome II. MRP-S9 encodes for a hydrophilic and basic protein of 278 amino acids with a molecular mass of 32 kDa. The C-terminal part (aa 153-278) of the MRP-S9 protein exhibits significant sequence similar...

journal_title：Current genetics

authors： Kötter P,Entian KD

更新日期：1995-06-01 00:00:00

abstract：:By using the fluorochrome 4'-6-diamidino-2-phenylindole (DAPI) to stain DNA one can follow the pattern of events affecting plastid DNA which occur in the formation and maturation of individual zygotes of the green flagellate Chlamydomonas moewusii. This species, like C. reinhardi, expresses uniparental inheritance of ...

journal_title：Current genetics

authors： Coleman AW,Maguire MJ

更新日期：1983-06-01 00:00:00

abstract：:In the multicellular red alga Antithamnion spec. both rubisco genes (rbcL and rbcS) are encoded on the plastid DNA (ptDNA). Both genes are separated by a short A/T-rich spacer of 100 bp and are cotranscribed into an mRNA of approximately 2.7 kb. These findings are in extensive agreement with those obtained from two un...

journal_title：Current genetics

authors： Kostrzewa M,Valentin K,Maid U,Radetzky R,Zetsche K

更新日期：1990-12-01 00:00:00

abstract：:In biological systems, reactive oxygen species (ROS) represent 'double edged swords': as signaling molecules they are essential for proper development, as reactive agents they cause molecular damage and adverse effects like degeneration and aging. A well-coordinated control of ROS is therefore of key importance. Super...

journal_title：Current genetics

authors： Grimm C,Böhl L,Osiewacz HD

更新日期：2015-02-01 00:00:00

abstract：:Approximately, 1% of the genes in eukaryotic genomes encode for helicases, which make the number of helicases expressed in the cell considerably high. Helicases are motor proteins that participate in many central aspects of the nuclear and mitochondrial genomes, and based on their helicase motif conservation, they are...

journal_title：Current genetics

authors： Sabouri N

更新日期：2017-08-01 00:00:00

abstract：:The cloning of fga1, the gene encoding a G protein alpha subunit, was performed by standard PCR techniques and by screening a Fusarium oxysporum genomic library, using the PCR product as a probe. The full-length open reading frame spanned 1,059 nucleotides and the deduced primary structure of the protein (353 amino ac...

journal_title：Current genetics

authors： Jain S,Akiyama K,Mae K,Ohguchi T,Takata R

更新日期：2002-09-01 00:00:00

abstract：:Glutathione (GSH) is an abundantly produced low-molecular-weight (LMW) thiol in many organisms. However, a number of Gram-positive bacteria do not produce GSH, but instead produce bacillithiol (BSH) as one of the major LMW thiols. Similar to GSH, studies have found that BSH has various roles in the cell, including pro...

journal_title：Current genetics

authors： Rosario-Cruz Z,Boyd JM

更新日期：2016-02-01 00:00:00

abstract：:Antimycin-resistant (AR) mutants of the yeast Kluyveromyces lactis, obtained either spontaneously or after manganese treatment, were isolated and genetically characterized. Most of the mutants obtained after manganese mutagenesis and two spontaneous mutants, tolerated high antimycin concentrations (more than 10 microg...

journal_title：Current genetics

authors： Brunner A,Mendoza V,Tuena de Cobos A

更新日期：1987-01-01 00:00:00

abstract：:Mutants resistant to 200 micrograms/ml of the alkaloid lycorine (LYCR) in non-fermentable substrate were isolated after nitrosoguanidine mutagenesis. Tetrad analysis and growth of heterozygous (LYCR/lycS) diploids from two different mutants revealed that a single nuclear and dominant mutation is responsible for the re...

journal_title：Current genetics

authors： Del Giudice L,Massardo DR,Manna F,Wolf K

更新日期：1986-01-01 00:00:00