当前位置： SCI文献检索 > CURRENT GENETICS期刊下所有文献 > RNA silencing in Chlamydomonas: mechanisms and tools.

RNA silencing in Chlamydomonas: mechanisms and tools.

Abstract：

:The generation of a comprehensive EST library and the sequencing of its genome set the stage for reverse genetics approaches in Chlamydomonas reinhardtii. However, these also require tools for the specific downregulation of target gene expression. Consequently, a large number of diverse constructs were developed aimed at reducing target gene expression in Chlamydomonas via the stable expression of antisense or inverted repeat-containing RNA. Double-stranded RNA (dsRNA) generated by the annealing of antisense and sense RNAs or by hairpin formation of an inverted repeat, feeds into the RNA silencing pathway. In this pathway, dsRNA is cleaved into approximately 25-bp small interfering RNAs (siRNAs) by the endonuclease Dicer. One of the two complementary strands of a siRNA is then loaded onto an Argonaute-like protein present as core component within larger complexes. Guided by this single-stranded RNA, the Argonaute-like protein either detects homologous transcripts and cleaves these endonucleolytically, or initiates transcriptional gene silencing. This article summarizes current information derived mainly from the Chlamydomonas genome project on components that are assumed to be involved in RNA silencing mechanisms in Chlamydomonas. Furthermore, all approaches employed in Chlamydomonas to date to downregulate target gene expression by antisense or inverted repeat constructs are reviewed and discussed critically.

journal_name

Curr Genet

journal_title

Current genetics

authors

Schroda M

doi

10.1007/s00294-005-0042-1

subject

Has Abstract

pub_date

2006-02-01 00:00:00

pages

69-84

issue

2

eissn

0172-8083

issn

1432-0983

journal_volume

49

pub_type

杂志文章,评审

相关文献

CURRENT GENETICS文献大全
  • Loss of 2 um DNA from Saccharomyces cerevisiae transformed with the chimaeric plasmid pJDB219.

    abstract：:Two plasmids containing Saccharomyces cerevisiae 2 µm DNA sequences and the S. cerevisiae LEU2 gene have been found to display incompatibility with 2 µm DNA; in the presence of the LEU2 plasmids, 2 µm DNA can be lost. The LEU2 plasmids can be lost spontaneously after (and before) 2 µm DNA loss has occurred, so that st...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00435686

    authors： Dobson MJ,Futcher AB,Cox BS

    更新日期：1980-12-01 00:00:00

  • Natural translocation of a large segment of chromosome III to chromosome I in a laboratory strain of Saccharomyces cerevisiae.

    abstract：:We have investigated chromosomal segregation during meiosis in a cross between two polymorphic haploid laboratory strains of Saccharomyces cerevisiae, FL100 and GRF18. These two strains have large chromosome-length polymorphisms for chromosomes I and III allowing for easy scoring of parental chromosomes after meiotic ...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/s002940050124

    authors： Camasses A

    更新日期：1996-08-01 00:00:00

  • The functions of the multi-tasking Pfh1Pif1 helicase.

    abstract：:Approximately, 1% of the genes in eukaryotic genomes encode for helicases, which make the number of helicases expressed in the cell considerably high. Helicases are motor proteins that participate in many central aspects of the nuclear and mitochondrial genomes, and based on their helicase motif conservation, they are...

    journal_title：Current genetics

    pub_type： 杂志文章,评审

    doi：10.1007/s00294-016-0675-2

    authors： Sabouri N

    更新日期：2017-08-01 00:00:00

  • Regulation of mitochondrial transcription during the stringent response in yeast.

    abstract：:In yeast (S. cerevisiae) the stringent response is known to include rapid, selective, and severe transcriptional curtailment for genes specifying cytoplasmic rRNAs and r-proteins. We have shown that transcription of the mitochondrial 21S rRNA gene is also congruently and selectively curtailed during the yeast stringen...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00336848

    authors： Cantwell R,McEntee CM,Hudson AP

    更新日期：1992-03-01 00:00:00

  • Multiple group I introns detected in the nuclear small subunit rDNA of the autosporic green alga Selenastrum capricornutum.

    abstract：:A phylogenetic investigation of the autosporic chlorophycean alga species Selenastrum capricornutum using the small subunit (SSU) rRNA gene revealed the unusual presence of six group IC1 introns. Previous studies showed that numerous green algal taxa contain group IC1 introns. However, whereas some algal species harbo...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/s00294-004-0525-5

    authors： Booton GC,Floyd GL,Fuerst PA

    更新日期：2004-10-01 00:00:00

  • The use of RAPD markers in the genetic analysis of the plant pathogenic fungus Cladosporium fulvum.

    abstract：:RAPD markers have been obtained in Cladosporium fulvum, in order to establish a genetic map based on mitotic recombination in this imperfect fungus. Segregation analysis has provided molecular evidence of a high degree of recombination during the parasexual cycle, and of the ploidy level of the parasexual progeny. A m...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00351783

    authors： Arnau J,Housego AP,Oliver RP

    更新日期：1994-05-01 00:00:00

  • Cloning and expression of Hormoconis resinae glucoamylase P cDNA in Saccharomyces cerevisiae.

    abstract：:A cDNA coding for glucoamylase P of Hormoconis resinae was cloned using a synthetic oligonucleotide probe coding for a peptide fragment of the purified enzyme and polyclonal anti-glucoamylase antibodies. Nucleotide-sequence analysis revealed an open reading frame of 1848 base pairs coding for a protein of 616 amino-ac...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00324663

    authors： Vainio AE,Torkkeli HT,Tuusa T,Aho SA,Fagerström BR,Korhola MP

    更新日期：1993-07-01 00:00:00

  • Arenicin-1-induced apoptosis-like response requires RecA activation and hydrogen peroxide against Escherichia coli.

    abstract：:Arenicin-1, a 21-mer antimicrobial peptide exerts significant broad-spectrum antimicrobial activity with membrane-active mechanisms. However, owing to multiple mechanisms of cell death, the antibacterial mechanism of arenicin-1 requires detailed analysis. In the present study, arenicin-1-treated bacteria underwent an ...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/s00294-018-0855-3

    authors： Lee H,Lee DG

    更新日期：2019-02-01 00:00:00

  • The genetic fine structure of nonsense suppressors in Schizosaccharomyces pombe : II. sup8 and sup10.

    abstract：:Meiotic fine-structure maps of two efficient UGA suppressors of Schizosaccharomyces pombe which are known (sup8-e) or inferred (sup10-e) to code for two leucine tRNAs carrying the mutant anticodon U(*)CA (Kohli et al. 1979, 1980a, b; Wetzel et al. 1979; Mao et al. 1981) are presented. In both cases, the recombination ...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00365633

    authors： Munz P,Dorsch-Häsler K,Leupold U

    更新日期：1983-04-01 00:00:00

  • Brassica nap cytoplasmic male sterility is associated with expression of a mtDNA region containing a chimeric gene similar to the pol CMS-associated orf224 gene.

    abstract：:Two different cytoplasmic male-sterility (CMS) systems, nap and pol, are found in the oilseed rape (canola) species Brassica napus. Physical mapping studies have previously shown that organizational differences between the sterile pol and fertile cam mitochondrial genomes are restricted to a relatively small region im...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/s002940050212

    authors： L'Homme Y,Stahl RJ,Li XQ,Hameed A,Brown GG

    更新日期：1997-04-01 00:00:00

  • Chromosome loading of cohesin depends on conserved residues in Scc3.

    abstract：:Cohesin is essential for sister chromatid cohesion, which ensures equal segregation of the chromatids to daughter cells. However, the molecular mechanism by which cohesin mediates this function is elusive. Scc3, one of the four core subunits of cohesin, is vital to cohesin activity. However, the mechanism by which Scc...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/s00294-020-01150-3

    authors： Pathania A,Liu W,Matityahu A,Irudayaraj J,Onn I

    更新日期：2021-01-06 00:00:00

  • New and efficient method using Saccharomyces cerevisiae mutants for identification of siderophores produced by microorganisms.

    abstract：:The separation and identification of siderophores produced by microorganisms is a time-consuming and an expensive procedure. We have developed a new and efficient method to identify siderophores using well-established Saccharomyces cerevisiae deletion mutants. The Deltafet3,arn strains fail to sustain growth, even whe...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/s00294-007-0145-y

    authors： Park YS,Kim JH,Chang HI,Kim SW,Paik HD,Kang CW,Kim TH,Sung HC,Yun CW

    更新日期：2007-09-01 00:00:00

  • A novel nuclear gene, CBT1, essential for mitochondrial cytochrome b formation: terminal processing of mRNA and intron dependence.

    abstract：:We describe a new nuclear gene, CBT1 (Cytochrome B Termination), specifically involved in the generation of mature mRNA of cytochrome b in yeast mitochondria. Disruption of CBT1 (corresponding to ORF YKL 208W) results in a respiratory deficiency (no growth on acetate and ethanol, a reduced growth on glycerol, and a mo...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/s002940050262

    authors： Rieger KJ,Aljinovic G,Lazowska J,Pohl TM,Slonimski PP,Aljinovic G

    更新日期：1997-09-01 00:00:00

  • Genetic map of mitochondrial DNA in Podospora anserina.

    abstract：:In order to develop an eukaryotic vector with the Podospora plasmid, further characterization is required of the mitochondrial DNA into which this plasmid is integrated, a physical map (restriction sites) of the Podospora chondriome (size 95 kb) has been completed. As prerequisite for the establishment of a genetic (f...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00365705

    authors： Kück U,Esser K

    更新日期：1982-07-01 00:00:00

  • Expression of Tri15 in Fusarium sporotrichioides.

    abstract：:In the fungus Fusarium sporotrichioides, biosynthesis of trichothecene mycotoxins requires at least three genetic loci: a core 12-gene cluster, a smaller two-gene cluster, and a single-gene locus. Here, we describe the Tri15 gene, which represents a fourth locus involved in trichothecene biosynthesis. Tri15 is predict...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/s00294-003-0467-3

    authors： Alexander NJ,McCormick SP,Larson TM,Jurgenson JE

    更新日期：2004-03-01 00:00:00

  • Interactions Between the MAT locus and the rad52-1 mutation in yeast.

    abstract：:The directed and controlled switching of mating type which occurs in homothallic forms of the yeast Saccharomyces cerevisiaee is dependent upon the presence of the wild type RAD52 gene. The RAD52 gene is also required for spontaneous mitotic and meiotic recombination. It has been observed that the two haploid mating t...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00377609

    authors： Malone RE,Hyman D

    更新日期：1983-11-01 00:00:00

  • HBP2: a new mammalian protein that complements the fission yeast MBF transcription complex.

    abstract：:The mammalian HBP2 gene has been isolated by functional complementation of cells unable to undergo DNA replication in fission yeast. HBP2 is a protein with a high mobility group (HMG) domain that belongs to the Sox (Sry-related HMG box) family of transcription factors. As in other members of the family, the HMG box in...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/s002940100241

    authors： Sánchez-Díaz A,Blanco MA,Jones N,Moreno S

    更新日期：2001-09-01 00:00:00

  • Characterization of thermosensitive mutants of Physarum polycephalum a plasmodial screening method for cell cycle mutants based upon synchronization at the restrictive temperature.

    abstract：:A screening procedure for cell cycle mutants among is mutants of Physarum has been developed for unsynchronized microplasmodia. The synchronization of the microplasmodia and the ratio of pre- to post-mitotic nuclei were evaluated after a transient shift-up to the non-permissive temperature. In contrast with wild type ...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00390945

    authors： Wright M,Del Castillo L,Oustrin ML

    更新日期：1980-04-01 00:00:00

  • DNA Topoisomerase II modulates acetyl-regulation of cohesin-mediated chromosome dynamics.

    abstract：:Cohesin is one of three multi-protein structural maintenance of chromosome (SMC) complexes that regulate eukaryotic chromosome dynamics. It forms a ring-shaped structure that embraces sister chromatids through interphase to promote their pairing. In preparation for mitosis, most cohesin is stripped from the chromosome...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/s00294-017-0691-x

    authors： Lin SJ,O'Connell MJ

    更新日期：2017-10-01 00:00:00

  • A 7.5-kbp region of the maize (T cytoplasm) mitochondrial genome contains a chloroplast-like trnI (CAT) pseudo gene and many short segments homologous to chloroplast and other known genes.

    abstract：:The DNA sequence of a 7.5-kbp region from the maize cmsT mt genome was determined. Nucleotide-sequence analysis of this region reveals the presence of a ct-like trnI (CAT) gene, orf31 and orf48 which are respectively homologous to the trnI (CAT) gene in the maize and rice chloroplast genomes, and to orf28 and orf64 in...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/s002940050256

    authors： Zheng D,Nielsen BL,Daniell H

    更新日期：1997-08-01 00:00:00

  • Alternative transcription start sites of the enolase-encoding gene enoA are stringently used in glycolytic/gluconeogenic conditions in Aspergillus oryzae.

    abstract：:Gene expression using alternative transcription start sites (TSSs) is an important transcriptional regulatory mechanism for environmental responses in eukaryotes. Here, we identify two alternative TSSs in the enolase-encoding gene (enoA) in Aspergillus oryzae, an industrially important filamentous fungus. TSS use in e...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/s00294-020-01053-3

    authors： Inoue T,Toji H,Tanaka M,Takama M,Hasegawa-Shiro S,Yamaki Y,Shintani T,Gomi K

    更新日期：2020-08-01 00:00:00

  • Mutations at three different nuclear loci of Chlamydomonas suppress a defect in chloroplast psbD mRNA accumulation.

    abstract：:In the photosynthetic chloroplast mutant PRB2A of Chlamydomonas reinhardtii the psbD mRNA is unstable. Three strains were isolated, in which the underlying site-directed mutation within the psbD 5' UTR (untranslated region) is suppressed. In all three suppressors, psbD RNA levels and RNA 5' maturation are restored to ...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/s002940050020

    authors： Nickelsen J

    更新日期：2000-02-01 00:00:00

  • Cloning and analysis of the nuclear gene MRP-S9 encoding mitochondrial ribosomal protein S9 of Saccharomyces cerevisiae.

    abstract：:The Saccharomyces cerevisiae nuclear gene MRP-S9 was identified as part of the European effort in sequencing chromosome II. MRP-S9 encodes for a hydrophilic and basic protein of 278 amino acids with a molecular mass of 32 kDa. The C-terminal part (aa 153-278) of the MRP-S9 protein exhibits significant sequence similar...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00311878

    authors： Kötter P,Entian KD

    更新日期：1995-06-01 00:00:00

  • Recent insights into Candida albicans biofilm resistance mechanisms.

    abstract：:Like other microorganisms, free-living Candida albicans is mainly present in a three-dimensional multicellular structure, which is called a biofilm, rather than in a planktonic form. Candida albicans biofilms can be isolated from both abiotic and biotic surfaces at various locations within the host. As the number of a...

    journal_title：Current genetics

    pub_type： 杂志文章,评审

    doi：10.1007/s00294-013-0400-3

    authors： Mathé L,Van Dijck P

    更新日期：2013-11-01 00:00:00

  • Gene overexpression screen for chromosome instability in yeast primarily identifies cell cycle progression genes.

    abstract：:Loss of heterozygosity (LOH) in a vegetatively growing diploid cell signals irregularity of mitosis. Therefore, assays of LOH serve to discover pathways critical for proper replication and segregation of chromosomes. We screened for enhanced LOH in a whole-genome collection of diploid yeast strains in which a single g...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/s00294-018-0885-x

    authors： Tutaj H,Pogoda E,Tomala K,Korona R

    更新日期：2019-04-01 00:00:00

  • Molecular cloning and analysis of the nuclear gene MRP-L6 coding for a putative mitochondrial ribosomal protein from Saccharomyces cerevisiae.

    abstract：:The Saccharomyces cerevisiae nuclear gene MRP-L6 was cloned by complementation of the respiratory-deficient mutant pet-ts 2523 with a library of wild-type yeast genomic DNA. The isolated gene was part of a 3.8-kb sequenced DNA fragment containing, in addition to MRP-L6, two unassigned reading frames, ORF1 and ORF2. MR...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00324677

    authors： Harrer R,Schwank S,Schüller HJ,Schweizer E

    更新日期：1993-07-01 00:00:00

  • Molecular cloning of the yeast OPI3 gene as a high copy number suppressor of the cho2 mutation.

    abstract：:By functional complementation of the auxotrophic requirements for choline of a cdg1, cho2 double-mutant, by transformation with a genomic DNA library in a high copy number plasmid, two different types of complementing DNA inserts were identified. One type of insert was earlier shown to represent the CHO2 structural ge...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00352006

    authors： Preitschopf W,Lückl H,Summers E,Henry SA,Paltauf F,Kohlwein SD

    更新日期：1993-02-01 00:00:00

  • The fate of mitochondrial DNAs of mt+ and mt- origin in gametes and zygotes of Chlamydomonas.

    abstract：:In order to study the mechanism responsible for the uniparental transmission of the mitochondrial genome in crosses between Chlamydomonas reinhardtii and C. smithii, we have analyzed the fate of mitochondrial DNA during gametogenesis, zygospore differentiation and sporulation by hybridization experiments. Both mt+ and...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00326238

    authors： Beckers MC,Munaut C,Minet A,Matagne RF

    更新日期：1991-08-01 00:00:00

  • The influence of the Ustilago maydis REC1 gene on plasmid-chromosome recombination.

    abstract：:The REC1 gene of U. maydis has an important but ill-defined role in DNA recombination and repair. We have examined its role in plasmid-chromosome recombination. Plasmid DNA was linearized at various locations with respect to the cloned U. maydis PYR3 gene and introduced into cells by transformation. Chromosomal integr...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00326414

    authors： Banks GR,Kanuga N,Ealing J,Spanos A,Holden DW

    更新日期：1992-12-01 00:00:00

  • Extragenic revertants of rad50, a yeast mutation causing defects in recombination and repair.

    abstract：:The RAD50 gene in yeast is required for recombination-repair (i.e., the double strand break repair pathway) in mitosis, and for meiotic recombination and sporulation. Both of these processes are complex and seem likely to require a relatively large number of gene products. In order to help define other genes required ...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00434050

    authors： Malone RE,Jordan K,Wardman W

    更新日期：1985-01-01 00:00:00