The use of RAPD markers in the genetic analysis of the plant pathogenic fungus Cladosporium fulvum.

abstract：:The potato (Solanum tuberosum L.) mitochondrial cox3/ sdh4 gene cluster was previously identified by heterologous hybridization using a Marchantia polymorpha sdh4 probe. In this work, this potato gene cluster was cloned, sequenced and its expression was evaluated. The gene sequence and gene locus organization were fou...

journal_title：Current genetics

authors： Siqueira SF,Dias SM,Hardouin P,Pereira FR,Lejeune B,de Souza AP

更新日期：2002-07-01 00:00:00

abstract：:In order to study pathogenic mechanisms of the plant pathogen Ascochyta rabiei, conditions for efficient transformation using Agrobacterium-mediated transformation were investigated. Hygromycin B resistance (hph) was superior to geneticin resistance (nptII) for selecting transformants, and the hph gene was more effici...

journal_title：Current genetics

authors： White D,Chen W

更新日期：2006-04-01 00:00:00

abstract：:Cohesin is one of three multi-protein structural maintenance of chromosome (SMC) complexes that regulate eukaryotic chromosome dynamics. It forms a ring-shaped structure that embraces sister chromatids through interphase to promote their pairing. In preparation for mitosis, most cohesin is stripped from the chromosome...

journal_title：Current genetics

authors： Lin SJ,O'Connell MJ

更新日期：2017-10-01 00:00:00

abstract：:The Cys6Zn2 DNA-binding domain transcription factor Pdr1 is a central regulator of drug resistance in the pathogenic yeast Candida glabrata. In this review, I discuss the multiple control mechanisms modulating the function of this positive transcriptional regulator. Available data suggest that Pdr1 activity is restrai...

journal_title：Current genetics

authors： Moye-Rowley WS

更新日期：2019-02-01 00:00:00

abstract：:Using differential hybridization, a gene preferentially expressed during entry into stationary phase has been isolated. Subsequent analysis indicated that this gene corresponds to a heat-shock gene. The nucleotide sequence has been determined. It revealed a 332 amino-acid protein. No similarities to any previously kno...

journal_title：Current genetics

authors： Régnacq M,Boucherie H

更新日期：1993-05-01 00:00:00

abstract：:Zearalenone (ZEA) is a secondary metabolite produced by various Fusarium species and causes estrogenic disorders in humans and animals. Recent studies have identified the ZEA biosynthesis gene cluster in F. graminearum, but other genes such as transporters responsible for ZEA export have not been identified in the clu...

journal_title：Current genetics

authors： Lee S,Son H,Lee J,Lee YR,Lee YW

更新日期：2011-10-01 00:00:00

abstract：:Unlike most polymorphic markers in the Chlamydomonas eugametos and Chlamydomonas moewusii chloroplast DNAs (cpDNAs), the C. moewusii 6- and 21-kb extra sequences and the C. eugametos-specific CeLSU small middle dot 5 intron are transmitted to all of the few viable progeny in reciprocal crosses between the two green al...

journal_title：Current genetics

authors： Bussières J,Lemieux C,Lee RW,Turmel M

更新日期：1996-09-01 00:00:00

abstract：:A class of large site-specific deletions (del-B) occurs with exceptionally-high frequencies of 10(-3) in the mitochondrial COX1 gene of Mn(2+)-treated yeast cells. This work shows that del-B deletions are associated with COX1 intron aI1. All five deletion mutants studied have their upstream end at the authentic 3' spl...

journal_title：Current genetics

authors： Weiller GF

更新日期：1994-11-01 00:00:00

abstract：:Six ultra violet (UV) mutageneses were performed on the spo76 UV-sensitive mutant of Sordaria macrospora. Spo76 shows an early centromere cleavage associated with an arrest at the first meiotic division and therefore does not form ascospores. Moreover, it exhibits altered pairing structure (synaptonemal complex), reve...

journal_title：Current genetics

authors： Huynh AD,Leblon G,Zickler D

更新日期：1986-01-01 00:00:00

abstract：:The ornithine transaminase (otaA) gene of Aspergillus nidulans has been cloned by transformation of the A. nidulans pro-ota- mutant strain with a cosmid gene library. The otaA gene contains two introns and potentially codes for a 453-aa-long protein. The deduced amino-acid sequence is homologous to known ornithine tra...

journal_title：Current genetics

authors： Dzikowska A,Swianiewicz M,Talarczyk A,Wisniewska M,Goras M,Scazzocchio C,Weglenski P

更新日期：1999-03-01 00:00:00

abstract：:Botryotinia fuckeliana, the causal agent of grey mould, was biolistically transformed to hygromycin B resistance using a plasmid (pOHT) containing a bacterial hygromycin phosphotransferase gene fused to regulatory sequences from Aspergillus nidulans. Multiple copies of the plasmid, precipitated onto tungsten particles...

journal_title：Current genetics

authors： Hilber UW,Bodmer M,Smith FD,Köller W

更新日期：1994-02-01 00:00:00

abstract：:In order to study resistance to DNA damaging agents, yeast DNA segments conferring hyperresistance in this organism to such genotoxic agents were selected for among yeast cells transformed by a yeast genome library based on the multi-copy vector plasmid YEp13. Genetic variants hyperresistant to 4-nitroquinoline-N-oxid...

journal_title：Current genetics

authors： Ruhland A,Brendel M,Haynes RH

更新日期：1986-01-01 00:00:00

abstract：:NRE, the nitrogen regulatory protein of Penicillium chrysogenum, contains a single Cys2/Cys2-type zinc-finger motif followed immediately by a highly basic region. The zinc-finger domain was expressed to Escherichia coli as a fusion protein with beta-galactosidase. In order to test the putative DNA-binding ability of N...

journal_title：Current genetics

authors： Haas H,Marzluf GA

更新日期：1995-07-01 00:00:00

abstract：:Phylum Euglenozoa comprises three groups of eukaryotic microbes (kinetoplastids, diplonemids, and euglenids), the mitochondrial (mt) genomes of which exhibit radically different modes of organization and expression. Gene fragmentation is a striking feature of both euglenid and diplonemid mtDNAs. To rationalize the eme...

journal_title：Current genetics

authors： Flegontov P,Gray MW,Burger G,Lukeš J

更新日期：2011-08-01 00:00:00

abstract：:Cell aging is the result of deteriorating competence in maintaining cellular homeostasis and quality control. Certain cell types are able to rejuvenate through asymmetric cell division by excluding aging factors, including damaged cellular compartments and extrachromosomal rDNA circles, from entering the daughter cell...

journal_title：Current genetics

authors： Koch-Bojalad BA,Carson L,Yu HG

更新日期：2020-11-27 00:00:00

abstract：:The endpoints of an inversion in wheat chloroplast DNA are shown to be associated with copies of a short repeated sequence. Recombination across the repeats in an inverted configuration may have been responsible for the inversion, although they are currently in a direct orientation owing to a second inversion. The rep...

journal_title：Current genetics

authors： Howe CJ

更新日期：1985-01-01 00:00:00

abstract：:In fungi, vegetative incompatibility is a conspecific non-self recognition mechanism that restricts formation of viable heterokaryons when incompatible alleles of specific het loci interact. In Podospora anserina, three non-allelic incompatibility systems have been genetically defined involving interactions between he...

journal_title：Current genetics

authors： Chevanne D,Bastiaans E,Debets A,Saupe SJ,Clavé C,Paoletti M

更新日期：2009-02-01 00:00:00

abstract：:The presence of the endochitinase CHIT36 from Trichoderma harzianum TM was assessed in several antagonistic Trichoderma strains belonging to different molecular taxonomic groups. CHIT37 from T. harzianum CECT 2413 was sequenced and found to display 89% homology with CHIT36 at the amino acid level. Northern analysis sh...

journal_title：Current genetics

authors： Viterbo A,Montero M,Ramot O,Friesem D,Monte E,Llobell A,Chet I

更新日期：2002-11-01 00:00:00

abstract：:International efforts are underway that aim at determining the complete genome sequence of the social amoeba Dictyostelium discoideum. As strategy, a whole chromosome shotgun (WCS) approach was chosen and each of the six Dictyostelium chromosomes was assigned to project partners. The project is well advanced, chromoso...

journal_title：Current genetics

authors： Eichinger L

更新日期：2003-11-01 00:00:00

abstract：:Timely progression of living cells through the cell cycle is precisely regulated. This involves a series of phosphorylation events which are regulated by various cyclins, activated in coordination with the cell cycle progression. Phosphorylated proteins govern cell growth, division as well as duplication of the geneti...

journal_title：Current genetics

authors： Dmowski M,Fijałkowska IJ

更新日期：2017-12-01 00:00:00

abstract：:Previous work in other laboratories has shown that several mutagen sensitive mutants of Neurospora crassa are extremely sensitive to low levels of histidine in the culture medium. We have shown that wild type Neurospora accumulates nicks or breaks in the DNA in the presence of histidine. The number of nicks accumulati...

journal_title：Current genetics

authors： Howard CA,Baker TI

更新日期：1988-05-01 00:00:00

abstract：:Deletion mutants for the yeast transketolase gene TKL1 were constructed by gene replacement. Transketolase activity was below the level of detection in mutant crude extracts. Transketolase protein could be detected as a single protein band of the expected size by Western-blot analysis in wild-type strains but not in t...

journal_title：Current genetics

authors： Schaaff-Gerstenschläger I,Zimmermann FK

更新日期：1993-11-01 00:00:00

abstract：:A main function of ectomycorrhizas, a symbiosis between certain soil fungi and fine roots of woody plants, is the exchange of plant-derived carbohydrates for fungus-derived nutrients. As it is required in large amounts, nitrogen is of special interest. A gene (AmAMT2) coding for a putative fungal ammonium importer was...

journal_title：Current genetics

authors： Willmann A,Weiss M,Nehls U

更新日期：2007-02-01 00:00:00

abstract：:In this work, we explore the use of the unbiased cDNA-AFLP strategy to identify genes involved in Mn(2+) homeostasis in Ceriporiopsis subvermispora. In this ligninolytic white-rot fungus, whose genome has not yet been sequenced, three Mn peroxidase genes responding to Mn(2+) have been characterized. Using cDNA-AFLP to...

journal_title：Current genetics

authors： Gutiérrez M,Rojas LA,Mancilla-Villalobos R,Seelenfreund D,Vicuña R,Lobos S

更新日期：2008-10-01 00:00:00

abstract：:We report the nucleotide sequence of three tRNA genes from maize mitochondria. The genes are located in two BamHI fragments, 3.55 and 5.7 kb long, adjacent to the S2 sequence in the maize mitochondrial genome. On the 3.55 kb BamHI fragment, we have characterized a tRNA(Cys)(GCA) gene. A strong sequence homology of thi...

journal_title：Current genetics

authors： Wintz H,Grienenberger JM,Weil JH,Lonsdale DM

更新日期：1988-03-01 00:00:00

abstract：:Five haploid monokaryons of the basidiomycete Coprinus cinereus were already known to be unable to form sclerotia (asexual resting structures) on the vegetative monokaryotic mycelium. Genetic analyses had shown that four distinct genes (symbolised scl were represented, all being "recessive" to their sclerotium-produci...

journal_title：Current genetics

authors： Moore D

更新日期：1981-05-01 00:00:00

abstract：:Cellular reverse transcriptase-related (rvt) genes represent a novel class of reverse transcriptases (RTs), which are only distantly related to RTs of retrotransposons and retroviruses, but, similarly to telomerase RTs, are immobilized in the genome as single-copy genes. They have been preserved by natural selection t...

journal_title：Current genetics

authors： Yushenova IA,Arkhipova IR

更新日期：2018-12-01 00:00:00

abstract：:Phyto-pathogenic fungi can cause huge damage to crop production. During millions of years of coexistence, fungi have evolved diverse life-style to obtain nutrients from the host and to colonize upon them. They deploy various proteinaceous as well as non-proteinaceous secreted molecules commonly referred as effectors t...

journal_title：Current genetics

authors： Pradhan A,Ghosh S,Sahoo D,Jha G

更新日期：2020-11-04 00:00:00

abstract：:Prototrophic hybrids have been obtained by the fusion of auxotrophic haploid strains of the two yeasts Yarrowia (Saccharomycopsis) lipolytica and Kluyveromyces lactis. The hybrid fusants had a colonial morphology intermediate between that of the two parent strains, were uninucleate, and contained an approximately dipl...

journal_title：Current genetics

authors： Groves DP,Oliver SG

更新日期：1984-01-01 00:00:00

abstract：:1. Nuclear mutants lacking cytochrome c l spectrum have been isolated. 2. The electron flow through the respiratory chain of the mutants is defect in the cytochrome bc 1-segment. 3. Complementation analysis has yielded two major complementation groups. 4. Tetrade analysis has established the two complementation groups...

journal_title：Current genetics

authors： Lang BF,Kaudewitz F

更新日期：1982-12-01 00:00:00