Abstract:
:Neurospora crassa wild type genome shows DNA sequences which are homologous to the sequences present in the rRNA processing genes of the yeast Saccharomyces cerevisiae. Five such processing genes from yeast, viz., RNA1 through RNA5, cloned in plasmid pBR322 were transformed in Escherichia coli strain LE392. Southern blots containing DNAs from these clones were restricted with several restriction endonucleases along with DNAs from lambda phage, rice (plant) and neuroblastoma (animal), were hybridized with 32P-labelled nick-translated N. crassa nuclear DNA under very stringent conditions. Autoradiograms of these blots revealed that four yeast rRNA processing genes (RNA1, RNA2, RNA3, and RNA4) showed homology with N. crassa nuclear DNA but such analogs were not found in DNAs representing prokaryotes, phages, higher plants and animals.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Dutta SK,Verma M,Verma Mdoi
10.1007/BF00436884subject
Has Abstractpub_date
1987-01-01 00:00:00pages
225-9issue
3eissn
0172-8083issn
1432-0983journal_volume
12pub_type
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