Abstract:
:Genes are overexpressed when present in yeast cells on multicopy plasmids. Taking advantage of the protein amplification which results from this overexpression, a method has been developed for large scale detection of yeast genes on randomly cloned DNA sequences. It is based on the analysis, by two-dimensional gel electrophoresis, of the proteins from yeast cells transformed with a yeast genomic DNA library constructed in a multicopy vector. We demonstrate here the applicability of this method for exploring the yeast genome. In addition, we report results which suggest that this method may also be useful for detecting regulatory genes.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Thoraval D,Régnacq M,Neuville P,Boucherie Hdoi
10.1007/BF00318208subject
Has Abstractpub_date
1990-11-01 00:00:00pages
281-6issue
4eissn
0172-8083issn
1432-0983journal_volume
18pub_type
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