Abstract：
:A homologous transformation system for Aspergillus oryzae is described. The system is based on an A. oryzae strain deficient in orotidine-5'-phosphate decarboxylase (pyrG) and the vector pAO4-2, which contains a functional A. oryzae pyrG gene as selection marker. Transformation of the A. oryzae pyrG mutant with circular pAO4-2 resulted in the appearance of Pyr+ transformants at a frequency of up to 20 per micrograms of DNA, whereas with linear pAO4-2 up to 200 transformants per micrograms DNA were obtained. In 75% of the Pyr+ transformants recombination events had occurred at the pyrG locus, most of which (90%) resulted in insertion of one or two copies of the vector and the others (10%) in a replacement of the mutant allele by the wild-type allele. Vector pAO4-2 is also capable of transforming a corresponding mutant of Aspergillus niger. This transformation system was used to introduce into A. oryzae the heterologous and non-selectable bacterial genes lacZ, encoding beta-galactosidase, and uidA, encoding beta-glucuronidase. Using the Aspergillus nidulans gpdA promoter to drive bacterial gene expression in A. oryzae, relatively high levels of activity, as well as protein per se, as judged by western blot analyses, were obtained.
journal_name
Curr Genetjournal_title
Current geneticsauthors
de Ruiter-Jacobs YM,Broekhuijsen M,Unkles SE,Campbell EI,Kinghorn JR,Contreras R,Pouwels PH,van den Hondel CAdoi
10.1007/BF00391472subject
Has Abstractpub_date
1989-09-01 00:00:00pages
159-63issue
3eissn
0172-8083issn
1432-0983journal_volume
16pub_type
杂志文章相关文献
CURRENT GENETICS文献大全abstract：:Six ultra violet (UV) mutageneses were performed on the spo76 UV-sensitive mutant of Sordaria macrospora. Spo76 shows an early centromere cleavage associated with an arrest at the first meiotic division and therefore does not form ascospores. Moreover, it exhibits altered pairing structure (synaptonemal complex), reve...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00447389
更新日期：1986-01-01 00:00:00
abstract：:In the filamentous ascomycete fungus Podospora anserina, senescence is associated with dramatic changes of the juvenile mitochondrial genome. During senescence specific regions of the non-senescent genome are excised, ligated and amplified as a plasmid. In this paper, we report our studies on nuclear-mitochondrial ext...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00418411
更新日期：1986-01-01 00:00:00
abstract：:A transformation system utilizing Agrobacterium tumefaciens was developed for targeted gene disruption in Monilinia fructicola, a fungal pathogen that causes brown rot disease of stone fruits. Transformation with a vector containing the neomycin phosphotransferase II (nptII) cassette flanked with 4 kb cutinase gene (M...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s00294-006-0059-0
更新日期：2006-05-01 00:00:00
abstract：:We have further characterized the functionality of the Saccharomyces cerevisiae gene SLT2(MPK1), coding for a MAP-kinase homolog essential for cell integrity, which is involved in the Pkc1p signalling pathway. This gene was isolated on the basis of its capacity to complement the thermosensitive-autolytic, osmotic-reme...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF02426955
更新日期：1996-05-01 00:00:00
abstract：:In order to study the mechanism responsible for the uniparental transmission of the mitochondrial genome in crosses between Chlamydomonas reinhardtii and C. smithii, we have analyzed the fate of mitochondrial DNA during gametogenesis, zygospore differentiation and sporulation by hybridization experiments. Both mt+ and...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00326238
更新日期：1991-08-01 00:00:00
abstract：:DNA replication checkpoint is a cell signaling pathway that is activated in response to perturbed replication. Although it is crucial for maintaining genomic integrity and cell survival, the exact mechanism of the checkpoint signaling remains to be understood. Emerging evidence has shown that RecQ helicases, a large f...
journal_title：Current genetics
pub_type： 杂志文章,评审
doi：10.1007/s00294-020-01147-y
更新日期：2021-01-11 00:00:00
abstract：:A gene of Puccinia graminis f. sp. tritici, coding for the translation elongation factor 1 alpha (EF-1 alpha), was isolated from a P. graminis genomic library using the EF-1 alpha gene sequence of Absidia glauca. The coding region of 1389 nucleotides encodes a polypeptide of 463 amino acids and is interrupted by eight...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00352106
更新日期：1995-03-01 00:00:00
abstract：:Virus-like particles (VLPs) of 40 nm diameter were isolated from the yeast-like fungus Dipodascus magnusii. These VLPs copurify with several linear double-stranded RNA molecules of different size. We have found some polymorphism in both the length and the number of these dsRNAs among six D. magnusii strains. Analysis ...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00351499
更新日期：1993-03-01 00:00:00
abstract：:A 1,380-bp intervening sequence within the mitochondrial small subunit ribosomal RNA (mt SSU rRNA) gene of the fungus Sclerotinia sclerotiorum has been sequenced and identified as a group-I intron. This is the first report of an intron in the mt SSU rRNA gene. The intron shows close similarity in secondary structure t...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00313431
更新日期：1995-01-01 00:00:00
abstract：:The cytoplasmic virus-like element pWR1A from Debaryomyces robertsiae encodes a toxin (DrT) with similarities to the Pichia acaciae killer toxin PaT, which acts by importing a toxin subunit (PaOrf2) with tRNA anticodon nuclease activity into target cells. As for PaT, loss of the tRNA methyltransferase Trm9 or overexpr...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s00294-014-0426-1
更新日期：2014-08-01 00:00:00
abstract：:Chloroplast DNAs from six different laboratory collections of Euglena gracilis "strain Z" and var. bacillaris were analyzed with restriction endonucleases EcoRI and Bam HI. The most variable portion of the organelle genome is the region containing the ribosomal cistrons. Intraspecific differences occur in both ribosom...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00429820
更新日期：1981-07-01 00:00:00
abstract：:A molecular clone containing the complete sequence of a mitochondrial circular plasmid-like DNA (the R plasmid) isolated from the date-palm variety V3DP was used as a probe in Southern analyses of mitochondrial DNA prepared from other varieties. Another circular structure (the S plasmid) was detected in some of these ...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF02426965
更新日期：1996-05-01 00:00:00
abstract：:Somatic fusion between cells of Chlamydomonas containing complementing cell wall and auxotrophic mutations and bearing chloroplast markers for resistance to antibiotics (streptomycin or spectinomycin) have been performed to analyze the mode of chloroplast gene transmission in the fusion products. Prototrophic colonies...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00419578
更新日期：1981-04-01 00:00:00
abstract：:The PEL1 gene of Saccharomyces cerevisiae is essential for the cell viability of mitochondrial petite mutants, for the ability to utilize glycerol and ethanol on synthetic medium, and for cell growth at higher temperatures. By tetrad analysis the gene was assigned to chromosome III, centromere proximal of LEU2. The PE...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00336781
更新日期：1993-10-01 00:00:00
abstract：:Ribosome is responsible for protein synthesis and is able to monitor the sequence and structure of the nascent peptide. Such ability plays an important role in determining overall gene expression profile of the bacteria through ribosome stalling and rescuing. In this review, we briefly summarize our current understand...
journal_title：Current genetics
pub_type： 杂志文章,评审
doi：10.1007/s00294-015-0545-3
更新日期：2016-05-01 00:00:00
abstract：:Non-LTR retrotransposons (also known as LINEs) have had a major influence on the structure of many eukaryote genomes. They are abundant in many multicellular eukaryotes, including mammals, but appear to be absent from the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe. This absence has, to date, preclud...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s002940000181
更新日期：2001-04-01 00:00:00
abstract：:The separation and identification of siderophores produced by microorganisms is a time-consuming and an expensive procedure. We have developed a new and efficient method to identify siderophores using well-established Saccharomyces cerevisiae deletion mutants. The Deltafet3,arn strains fail to sustain growth, even whe...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s00294-007-0145-y
更新日期：2007-09-01 00:00:00
abstract：:Sequences hybridizing to mitochondrial DNA probes from Saccharomyces cerevisiae have been mapped in six mitochondrial genomes from the Dekkera/Brettanomyces yeasts and in mtDNA from the closely related Eeniella nana. Sequence order for the 34.5 kbp mtDNA of E. nana is identical to that for mtDNAs from B. custersianus ...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00418409
更新日期：1986-01-01 00:00:00
abstract：:The ribosomal proteins of two ascomycetes Podospora anserina and Saccharomyces cerevisiae, were compared by two dimensional electrophoresis in two different gel systems and we found only five pairs of proteins which have kept homologous physico-chemical properties under these conditions. An immunological analysis was ...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00396204
更新日期：1984-12-01 00:00:00
abstract：:In Saccharomyces cerevisiae, the HGS2-1 allele confers sensitivities to inorganis mercury (Ono and Sakamoto 1985) and to excess fermentable sugars such as glucose (Sakamoto et al. 1985); exogenous tyrosine antagonizes both inorganic mercury and excess glucose. In this study, the inorganic mercury sensitive strain has ...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00378183
更新日期：1987-01-01 00:00:00
abstract：:The yeast OMP decarboxylase gene has been cloned in the phage fd in both orientations to obtain pure DNA of each strand. With these probes the transcription of each strand of the yeast gene was measured in wild type yeast, either repressed or induced, in a yeast strain transformed by an hybrid plasmid containing the O...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00420621
更新日期：1980-10-01 00:00:00
abstract：:When the beta 1-, beta 2- and beta 3-tubulin-specific DNAs from Drosophila melanogaster were used as probes to recognize tubulin-specific sequences in the chromosomes of Drosophila auraria, they were found to hybridize to the same polytene band in region 32C of the 2L polytene chromosome. Three overlapping clones were...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00712973
更新日期：1994-01-01 00:00:00
abstract：:The presence of the endochitinase CHIT36 from Trichoderma harzianum TM was assessed in several antagonistic Trichoderma strains belonging to different molecular taxonomic groups. CHIT37 from T. harzianum CECT 2413 was sequenced and found to display 89% homology with CHIT36 at the amino acid level. Northern analysis sh...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s00294-002-0345-4
更新日期：2002-11-01 00:00:00
abstract：:Stress granules (SGs) are evolutionarily conserved ribonucleoprotein (RNP) structures that form in response to a variety of environmental and cellular cues. The presence of these RNP granules has been linked to a number of human diseases, including neurodegenerative disorders like amyotrophic lateral sclerosis (ALS) a...
journal_title：Current genetics
pub_type： 杂志文章,评审
doi：10.1007/s00294-016-0571-9
更新日期：2016-08-01 00:00:00
abstract：:Mutants which carry a leaky UGA nonsense mutation in the fertility gene ste10 are characterized by a deformed cell morphology which resembles that described in the literature for sterile ras1- (ste5) and ral1 to ral4 mutant cells. Although frequent conjugation attempts are observed in combinations of two ste10 mutant ...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00312768
更新日期：1991-07-01 00:00:00
abstract：:RAPD markers have been obtained in Cladosporium fulvum, in order to establish a genetic map based on mitotic recombination in this imperfect fungus. Segregation analysis has provided molecular evidence of a high degree of recombination during the parasexual cycle, and of the ploidy level of the parasexual progeny. A m...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00351783
更新日期：1994-05-01 00:00:00
abstract：:Cochliobolus heterostrophus has alternate genes (MAT-1 and MAT-2) at its mating-type locus. Transformants of a MAT-1 or a MAT-2 strain carrying a transgene of opposite mating type can self and are dual maters; the transgene, however, promotes development of pseudothecia only, not ascospores. To determine if the reside...
journal_title：Current genetics
pub_type： 杂志文章
doi：
更新日期：1996-02-01 00:00:00
abstract：:Transforming DNAs form cointegrates in Aspergillus nidulans by homologous and non-homologous recombination as well as by end-to-end ligation of linear fragments. This process has been studied by means of a model in which the linkage of a marker gene to the origin of autonomous replication AMA1 was selected for. Recomb...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00310500
更新日期：1994-10-01 00:00:00
abstract：:The bacterial hexameric helicase known as Rho is an archetypal sequence-specific transcription terminator that typically halts the synthesis of a defined set of transcripts, particularly those bearing cytosine-rich 3'-untranslated regions. However, under conditions of translational stress, Rho can also terminate trans...
journal_title：Current genetics
pub_type： 杂志文章,评审
doi：10.1007/s00294-019-00939-1
更新日期：2019-06-01 00:00:00
abstract：:It is now established that base-pairing regulatory RNAs are key players in post-transcriptional regulatory networks where they affect the translation and/or stability of their target RNAs. In many cases, the base-pairing between two RNAs is facilitated by an RNA-binding protein (RBP) that serves as an RNA chaperone. R...
journal_title：Current genetics
pub_type： 杂志文章,评审
doi：10.1007/s00294-020-01066-y
更新日期：2020-08-01 00:00:00
