Abstract:
:The complete nucleotide sequence of one of three linear DNA plasmids (pRS64-2) from the plant pathogenic fungus Rhizoctonia solani was determined. The pRS64-2 DNA consisted of 2877 nucleotides. The nucleotide sequences of the middle 2.2-kb regions of the other two plasmids (pRS64-1 and pRS64-3) were also determined. Comparison of the nucleotide sequences among the three plasmid DNAs indicated the presence of four regions with more than 86% sequence homology, suggesting the development of three plasmid DNAs from a common ancestor. A computer-based study of the pRS64-2 DNA-folding at both termini predicted hairpin loop structures. The hairpin loops consisted of the left- and right-hand terminal 113 and 105 nucleotides, respectively, and had no sequence homology. They had not undergone flip-flop inversion. The hairpins form cruciform base-paired structures.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Katsura K,Suzuki F,Miyashita SI,Nishi T,Hirochika H,Hashiba Tdoi
10.1007/s002940050299subject
Has Abstractpub_date
1997-12-01 00:00:00pages
431-5issue
6eissn
0172-8083issn
1432-0983journal_volume
32pub_type
杂志文章相关文献
CURRENT GENETICS文献大全abstract::The var1 locus on yeast mitochondria) DNA is defined by a set of alleles that affect the apparent size (Mr 40,000-44,000) of a mitochondria) translation product (var1), a protein of the mitochondria) 38S ribosomal subunit. Using petite deletion and restriction endonuclease site mapping, we have defined the physical lo...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00445691
更新日期:1980-07-01 00:00:00
abstract::Most organisms prefer to utilize glucose as a carbon source. Accordingly, the expression of genes involved in the catabolism of other carbon sources is repressed by the presence of glucose in a process known as (carbon) catabolite repression. However, much less is known about the relationships between "poor" carbon so...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-019-00996-6
更新日期:2019-12-01 00:00:00
abstract::Genetic and molecular analyses of the phenomenon of senescence-i.e., irreversible loss of growth and reproductive potential upon subculturing-in Neurospora intermedia strain M1991-60A, collected from Maddur in southern India, showed the presence of plasmid pMaddur1, which is homologous to the senescence-inducing circu...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-004-0558-9
更新日期:2005-03-01 00:00:00
abstract::We have used a PCR-based technique, involving the random amplification of polymorphic DNA (RAPD), to assess genome variability between 21 isolates from F. solani f. sp. cucurbitae races 1 and 2. Based on RAPD marker patterns the isolates fell into two distinct groups corresponding to mating populations MPI and MPV. Fo...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00317067
更新日期:1991-11-01 00:00:00
abstract::The influence of the DNA repair gene PSO3 on photoactivated psoralen-induced meiotic recombination, gene conversion, reverse mutation, and on survival, was assayed in diploid strains of Saccharomyces cerevisiae homozygous for the wild-type or the pso3-1 mutant allele. Sporulation was normal in the pso3-1 diploid. Wild...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00712961
更新日期:1994-01-01 00:00:00
abstract::In order to study resistance to DNA damaging agents, yeast DNA segments conferring hyperresistance in this organism to such genotoxic agents were selected for among yeast cells transformed by a yeast genome library based on the multi-copy vector plasmid YEp13. Genetic variants hyperresistant to 4-nitroquinoline-N-oxid...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00420609
更新日期:1986-01-01 00:00:00
abstract::We have isolated suppressors of a PHO4c (a positive regulator) mutant which normally confers weak constitutivity for acid phosphatase production on the Saccharomyces cell. One dominant suppressor (PHO80-2) was found to be an allele of PHO80 (a negative regulator) that changes G to A, resulting in substitution of isole...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00318466
更新日期:1992-02-01 00:00:00
abstract::To supply abundant ribosomes, multiple copies of ribosomal RNA genes (rDNA) are conserved from bacterial to human cells. In eukaryotic genomes, clusters of tandemly repeated rDNA units are present, and their number is stably maintained. Due to high level of transcription of rRNA genes, the repetitive structure is pron...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-019-00956-0
更新日期:2019-08-01 00:00:00
abstract::A cDNA coding for glucoamylase P of Hormoconis resinae was cloned using a synthetic oligonucleotide probe coding for a peptide fragment of the purified enzyme and polyclonal anti-glucoamylase antibodies. Nucleotide-sequence analysis revealed an open reading frame of 1848 base pairs coding for a protein of 616 amino-ac...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00324663
更新日期:1993-07-01 00:00:00
abstract::Many species are able to hybridize, but the sterility of these hybrids effectively prevents gene flow between the species, reproductively isolating them and allowing them to evolve independently. Yeast hybrids formed by Saccharomyces cerevisiae and Saccharomyces paradoxus parents are viable and able to grow by mitosis...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-019-01038-x
更新日期:2020-06-01 00:00:00
abstract::The mating-type ( MAT) genes from Rhynchosporium secalis were isolated using PCR-based methods. Characterisation of the MAT idiomorphs suggests that R. secalis is closely related to the discomycetes Pyrenopeziza brassicae and Tapesia yallundae in terms of sequence and MAT locus gene composition. The MAT1-2 idiomorph c...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-003-0445-9
更新日期:2003-12-01 00:00:00
abstract::We previously reported the isolation of mutants hypersensitive to the genotoxic agent 4-nitroquinoline-1-oxide, a potent inducer of oxidative stress. One of the mutants was defective in a gene designated yPTPA1, encoding a protein related to the human phosphotyrosyl phosphatase activator hPTPA, which is believed to pl...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-004-0513-9
更新日期:2004-08-01 00:00:00
abstract::Degenerative processes in the filamentous fungus Podospora anserina are strongly correlated with the instability of the mitochondrial genome. Among the sources of instability is the mobile group-II intron COX1-i1, also called intron alpha, which encodes a protein with a reverse transcriptase activity. In this paper we...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050003
更新日期:2000-01-01 00:00:00
abstract::In this work, we explore the use of the unbiased cDNA-AFLP strategy to identify genes involved in Mn(2+) homeostasis in Ceriporiopsis subvermispora. In this ligninolytic white-rot fungus, whose genome has not yet been sequenced, three Mn peroxidase genes responding to Mn(2+) have been characterized. Using cDNA-AFLP to...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-008-0209-7
更新日期:2008-10-01 00:00:00
abstract::Stress granules (SGs) are evolutionarily conserved ribonucleoprotein (RNP) structures that form in response to a variety of environmental and cellular cues. The presence of these RNP granules has been linked to a number of human diseases, including neurodegenerative disorders like amyotrophic lateral sclerosis (ALS) a...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-016-0571-9
更新日期:2016-08-01 00:00:00
abstract::The wheat mitochondrial (mt) cox3 has been localized and sequenced. The gene exists as a single copy in the wheat mt master chromosome and is transcribed into a single 1.2 kb RNA, whose extremities have been mapped. Comparison of the wheat and Oenothera cox3 sequences gives ambiguous indications concerning the amino a...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00313247
更新日期:1990-01-01 00:00:00
abstract::We have studied the splicing pathway leading to the synthesis of cytochrome oxidase subunit I (COX I) mRNA, by analysing the transcription pattern of several oxi3- splicing deficient mutants located in the first four introns of the gene. The four introns contain long open reading frames (ORFs) in phase with the upstre...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00389426
更新日期:1986-01-01 00:00:00
abstract::A simple method was developed for the conversion of homothallic Saccharomyces cerevisiae yeast strains to heterothallism through HO gene disruption. An integrative ho::neo disrupted allele was constructed by cloning a dominant selectable marker, the bacterial neo gene, within the HO gene. Transformation of a homothall...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00310889
更新日期:1993-01-01 00:00:00
abstract::Enzymatic and genetic characterization of cpd-1 and cpd-2, which exhibit rhythmic conidiation in liquid media and on solid media, were described with band (bd) strain as a reference. Cpd-1 and cpd-2 showed reduced growth in orthophosphate-free cyclic 3',5'-AMP media, while bd showed wild-type level of growth in the me...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00798749
更新日期:1985-01-01 00:00:00
abstract::The CYP1 gene has previously been identified as coding for a positive trans active factor that activates the expression of CYC1 and CYP3, which are the structural genes for isol1- and iso2-cytochrome c. Two phenotypically distinct classes of CYP1 mutations can be obtained indicating that CYC1 and CYP3 are differential...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00418404
更新日期:1986-01-01 00:00:00
abstract::Cellular reverse transcriptase-related (rvt) genes represent a novel class of reverse transcriptases (RTs), which are only distantly related to RTs of retrotransposons and retroviruses, but, similarly to telomerase RTs, are immobilized in the genome as single-copy genes. They have been preserved by natural selection t...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-018-0844-6
更新日期:2018-12-01 00:00:00
abstract::Phyto-pathogenic fungi can cause huge damage to crop production. During millions of years of coexistence, fungi have evolved diverse life-style to obtain nutrients from the host and to colonize upon them. They deploy various proteinaceous as well as non-proteinaceous secreted molecules commonly referred as effectors t...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-020-01118-3
更新日期:2020-11-04 00:00:00
abstract::Chromosomes of two closely related yeast strains, the amylolytic Saccharomyces diastaticus and the non-amylolytic Saccharomyces cerevisiae, were resolved by pulsed field gel electrophoresis (PFGE) and orthological field alteration gel electrophoresis (OFAGE). Electrophoretic karyotypes of these two strains are identic...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00405847
更新日期:1988-07-01 00:00:00
abstract::Microbial pathogens secrete an array of cell wall-degrading enzymes to break down the structure of the host cell wall to facilitate colonization of the host tissue. To better understand their role in the pathogenesis, a putative endoglucanase from Magnaporthe grisea was characterized in this paper. SignalP-3.0 analysi...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-008-0179-9
更新日期:2008-04-01 00:00:00
abstract::Mitochondrial genomes in organisms from diverse phylogenetic groups vary in both size and molecular form. Although the types of mitochondrial genome appear very dissimilar, several lines of evidence argue that they do not differ radically. This would imply that interconversion between different types of mitochondrial ...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-003-0426-z
更新日期:2003-11-01 00:00:00
abstract::A vector was constructed for transformation of the plant pathogenic fungus Fusarium solani. The promoter 35Sp, from cauliflower mosaic virus, was fused to the bacterial gene APH(3')II, which confers resistance to the aminoglycoside antibiotic G418. Two transformation procedures were developed: one using isolated funga...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00376799
更新日期:1989-06-01 00:00:00
abstract::Telomere maintenance mechanism is poorly studied in quiescence, a reversible non-proliferative state. We previously described in fission yeast a new mode of repair of telomeres named STEEx, that specifically operates in post-mitotic cells harboring eroded telomeres. This mechanism, promoted by transcription-induced te...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-018-0808-x
更新日期:2018-08-01 00:00:00
abstract::The ribosomal proteins of two ascomycetes Podospora anserina and Saccharomyces cerevisiae, were compared by two dimensional electrophoresis in two different gel systems and we found only five pairs of proteins which have kept homologous physico-chemical properties under these conditions. An immunological analysis was ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00396204
更新日期:1984-12-01 00:00:00
abstract::In Aspergillus nidulans, carbon catabolite repression (CCR) is mediated by the global repressor protein CreA. The deubiquitinating enzyme CreB is a component of the CCR network. Genetic interaction was confirmed using a strain containing complete loss-of-function alleles of both creA and creB. No direct physical inter...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-016-0667-2
更新日期:2017-08-01 00:00:00
abstract::RAPD markers have been obtained in Cladosporium fulvum, in order to establish a genetic map based on mitotic recombination in this imperfect fungus. Segregation analysis has provided molecular evidence of a high degree of recombination during the parasexual cycle, and of the ploidy level of the parasexual progeny. A m...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00351783
更新日期:1994-05-01 00:00:00