Abstract:
:Fifty isolates of Ascochyta rabiei (Pass.) Labr. were hierarchically sampled from four well-separated locations of a single chickpea field in Beja (Tunisia), and single-spored. DNA was isolated from in-vitro-grown mycelia, digested with HinfI or RsaI, and hybridized to a set of synthetic oligonucleotides complementary to simple repetitive sequences. According to the fingerprint patterns derived from the probes (CA)8, (CAA)5, (CAT)5 and (GATA)4, 12 different fungal haplotypes were found at various frequencies within the investigated field. Seven haplotypes were confined to one location only, four occurred at two, one at three, and none at all four locations. Most of the genetic variability originated from diversity within, rather than between, locations. In some cases, more than one haplotype was isolated from the same lesion of a single host plant. Genetic distances between isolates, as calculated from band-sharing data, varied between 0.05 and 0.22. Relatedness between the different haplotypes was evaluated by cluster analysis using UPGMA.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Morjane H,Geistlinger J,Harrabi M,Weising K,Kahl Gdoi
10.1007/BF00309547subject
Has Abstractpub_date
1994-09-01 00:00:00pages
191-7issue
3eissn
0172-8083issn
1432-0983journal_volume
26pub_type
杂志文章相关文献
CURRENT GENETICS文献大全abstract::The phosphate regulatory mechanism in yeast, known as the PHO pathway, is regulated by inorganic phosphate to control the expression of genes involved in the acquisition of phosphate from the medium. This pathway is also reported to contribute to other nutritional responses and as such it affects several phenotypic ch...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-005-0583-3
更新日期:2005-07-01 00:00:00
abstract::Killer protein 4 (KP4) is a well studied viral toxin secreted by the maize smut fungus Ustilago maydis that kills sensitive Ustilago strains as well as inhibits Fusarium and plant root growth by inhibiting calcium uptake. Numerous small, cysteine-rich proteins have been shown to play a critical role in fungal-plant-ba...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-010-0326-y
更新日期:2011-02-01 00:00:00
abstract::The amino acid sequences of two barley ribosomal proteins, termed HvL17-1 and HvL17-2, were decoded from green leaf cDNA clones. The N-terminal sequences of the derived barley proteins are 48% identical to the N-terminal amino acid sequence of protein YL17 from the large subunit of yeast cytoplasmic ribosomes. Via arc...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00309605
更新日期:1991-05-01 00:00:00
abstract::Ime1 plays a pivotal role in the initiation of meiosis in a/alpha diploid cells of Saccharomyces cerevisiae. In the absence of glucose and nitrogen, IME1 expression is greater in a/alpha cells than in either a or alpha cells and therefore only a/alpha, but not a/a or alpha/alpha, cells are committed to sporulation. It...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050332
更新日期:1998-04-01 00:00:00
abstract::An exceptional mode of mtDNA inheritance involving separate maternal and paternal transmission routes has been reported recently in the mussel Mytilus edulis. This mode of inheritance provides an explanation for the high levels of heteroplasmy for two highly diverged genomes observed in males of this species. Here we ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF02221509
更新日期:1996-04-01 00:00:00
abstract::This paper reports the mapping of both mature and precursor Paracentrotus lividus mitochondrial transcripts. Several mtRNAs were found to have 5' and 3' termini which differ from those inferred through DNA sequencing (Cantatore et al. 1989). The 3' ends of the two rRNAs (12S and 16S) overlap with the downstream transc...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00312615
更新日期:1990-03-01 00:00:00
abstract::Deletion mutants for the yeast transketolase gene TKL1 were constructed by gene replacement. Transketolase activity was below the level of detection in mutant crude extracts. Transketolase protein could be detected as a single protein band of the expected size by Western-blot analysis in wild-type strains but not in t...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00351843
更新日期:1993-11-01 00:00:00
abstract::Adaptation by natural selection might improve the fitness of an organism and its probability to survive in unfavorable environmental conditions. Decoding the genetic basis of adaptive evolution is one of the great challenges to deal with. To this purpose, Saccharomyces cerevisiae has been largely investigated because ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-016-0635-x
更新日期:2017-05-01 00:00:00
abstract::Chloroplast DNA from the fern Osmunda einnamomea was isolated by a sucrose gradient procedure utilizing PEG to stabilize chloroplasts. Analysis with the restriction endonucleases PvuII, Sacl and BstEII indicates a chloroplast genome size of 144 kb. A physical map of the fragments produced by these three enzymes was co...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00391801
更新日期:1982-08-01 00:00:00
abstract::RAPD markers have been obtained in Cladosporium fulvum, in order to establish a genetic map based on mitotic recombination in this imperfect fungus. Segregation analysis has provided molecular evidence of a high degree of recombination during the parasexual cycle, and of the ploidy level of the parasexual progeny. A m...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00351783
更新日期:1994-05-01 00:00:00
abstract::Botryotinia fuckeliana, the causal agent of grey mould, was biolistically transformed to hygromycin B resistance using a plasmid (pOHT) containing a bacterial hygromycin phosphotransferase gene fused to regulatory sequences from Aspergillus nidulans. Multiple copies of the plasmid, precipitated onto tungsten particles...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00309537
更新日期:1994-02-01 00:00:00
abstract::Antimycin-resistant (AR) mutants of the yeast Kluyveromyces lactis, obtained either spontaneously or after manganese treatment, were isolated and genetically characterized. Most of the mutants obtained after manganese mutagenesis and two spontaneous mutants, tolerated high antimycin concentrations (more than 10 microg...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00384609
更新日期:1987-01-01 00:00:00
abstract::Mitochondrial RNA from grande yeast was analyzed by electrophoresis on agarose-urea, acrylamide-urea, and methyl mercuric hydroxide-agarose gels. These gel systems display more than 40 RNA bands that copurify with mitochondria; these bands are not present in cytoplasmic RNA preparations. Analysis of molecular weight o...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00446962
更新日期:1980-02-01 00:00:00
abstract::Homologous recombination repairs discontinuities in DNA including single-strand gaps (SSGs) and double-strand breaks (DSBs). This commentary describes how the RecBCD and RecF pathways might be exchangeable for the repair of their respective DSB and SSG canonical substrates. In particular, I will discuss how the RecBCD...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-016-0575-5
更新日期:2016-08-01 00:00:00
abstract::Retrotransposons are activated as organisms age, based on work from several model systems. Since these mobile DNA elements can promote genome instability, this has raised the possibility that they can contribute to loss of cellular function with age. Many questions remain to be addressed about the relationship between...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-015-0538-2
更新日期:2016-05-01 00:00:00
abstract::Although the mitochondrial genomes of the Chinese Spring and Aquila varieties of wheat are normally similar in organization, this is not so in tissue cultures initiated from their immature embryos where the mitochondrial genomes of both are rearranged and in different, characteristic, ways. However, the mitochondrial ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00351662
更新日期:1992-05-01 00:00:00
abstract::A naturally occurring single base-pair G to A transition, creating a 10/11 near-match close to the essential 11 base-pair core consensus of ARS1, was used to investigate the importance of near-match sequences. The 10/11 near-match can not substitute for the core consensus since an ARS- phenotype is observed when the c...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00351723
更新日期:1992-09-01 00:00:00
abstract::Wild-type Neurospora crassa, strain Singapore, was transformed with a N. crassa metallothionein promoter/protyrosinase fusion gene. Transformants produced tyrosinase during vegetative growth, as determined by Western analyses and activity assays. This is in sharp contrast to wild-type strains, where this enzyme is onl...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00318214
更新日期:1990-11-01 00:00:00
abstract::The HOG pathway is an important mitogen-activated protein kinase (MAPK) signal transduction pathway in Saccharomyces cerevisiae that mediates adaptation of cells to hyper-osmotic stress. Activation of this pathway causes rapid but transient, phosphorylation of the MAPK Hog1p. Phosphorylated Hog1p is rapidly transporte...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-005-0010-9
更新日期:2005-09-01 00:00:00
abstract::To supply abundant ribosomes, multiple copies of ribosomal RNA genes (rDNA) are conserved from bacterial to human cells. In eukaryotic genomes, clusters of tandemly repeated rDNA units are present, and their number is stably maintained. Due to high level of transcription of rRNA genes, the repetitive structure is pron...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-019-00956-0
更新日期:2019-08-01 00:00:00
abstract::An Agrocybe aegerita cDNA library, constructed from fruit body primordia poly(A)+ RNAs, was screened by differential colony hybridization. Clones which preferentially hybridized to poly(A)+ RNA sequences from fruit body primordia, versus poly(A)+ RNAs from mycelium, were isolated. Eight of these clones (EMAa-1 to EMAa...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00326234
更新日期:1991-08-01 00:00:00
abstract::A double-stranded (ds)RNA, designated as M2, is associated with hypovirulence, conversion of the quinic acid pathway from inducible to constitutive and downregulation of the shikimic acid pathway in the Rhizoctonia solani culture Rhs 1A1. In this study, we report that in the virulent, M2-lacking isolate Rhs 1AP, which...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-003-0375-6
更新日期:2003-05-01 00:00:00
abstract::The ribosomal proteins of two ascomycetes Podospora anserina and Saccharomyces cerevisiae, were compared by two dimensional electrophoresis in two different gel systems and we found only five pairs of proteins which have kept homologous physico-chemical properties under these conditions. An immunological analysis was ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00396204
更新日期:1984-12-01 00:00:00
abstract::The GGP1 gene encodes the only GPI-anchored glycoprotein (gp115) that has been purified to-date in the budding yeast Saccharomyces cerevisiae. It is a single-copy gene whose deduced amino-acid sequence shares no significant homology to any other known protein. In this paper we report a Southern hybridization analysis ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00336744
更新日期:1993-01-01 00:00:00
abstract::Enzymatic and genetic characterization of cpd-1 and cpd-2, which exhibit rhythmic conidiation in liquid media and on solid media, were described with band (bd) strain as a reference. Cpd-1 and cpd-2 showed reduced growth in orthophosphate-free cyclic 3',5'-AMP media, while bd showed wild-type level of growth in the me...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00798749
更新日期:1985-01-01 00:00:00
abstract::The circular mitochondrial genome of Dictyostelium discoideum has a size of 55,564 base pairs. We present here a complete and detailed transcription map of the mitochondrial DNA. Eight major, polycistronic transcripts encoding polypeptides, ribosomal RNAs and interspersed transfer RNAs were identified in Northern hybr...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940100196
更新日期:2001-07-01 00:00:00
abstract::Cyclosporin A is a potent and clinically-important immunosuppressive drug (SandimmunR). It is produced by the fungus Tolypocladium niveum. A transformation system for T. niveum ATCC34921 based on hygromycin selection was established. In order to obtain a T. niveum promoter, the cyclophilin gene was isolated using the ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00309935
更新日期:1994-11-01 00:00:00
abstract::DNA polymerases are evolved to extend the 3'-OH of a growing primer annealed to a template DNA substrate. Since replicative DNA polymerases have a limited role while replicating structurally distorted template, translesion DNA polymerases mostly from Y-family come to the rescue of stalled replication fork and maintain...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-018-0918-5
更新日期:2019-06-01 00:00:00
abstract::As yeast colonies ceased growth, cells at the edge of these colonies transited from the cell division cycle into meiosis at high efficiency. This transition occurred remarkably synchronously and only at late stages of colony maturation. The transition occurred on medium containing acetate or low concentrations of gluc...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-002-0331-x
更新日期:2002-10-01 00:00:00
abstract::A screening of haploid yeast strains for enhanced resistance to nitrogen mustard (HN2) yielded a recessive mutant allele, hnm1, that conferred hyper-resistance (HYR) to HN2. Diploids, homo- or heterozygous for the HNM1 locus, exhibit normal wild-type like resistance while homozygosity for hnm1 leads to the phenotype H...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00318378
更新日期:1990-10-01 00:00:00