Abstract:
:Exonuclease II (ExoII) from Schizosaccharomyces pombe is a 5'-->3' single-stranded DNA exonuclease. We have cloned its gene, exo2, whose nucleotide sequence revealed that ExoII is a homologue of the multifunctional Saccharomyces cerevisiae Sep1 protein (also called Kem1, Xrn1, Rar5, Dst2). S. pombe exo2 null mutants were cold-sensitive for growth, had increased cell size at the restrictive temperature, were hypersensitive to the mitotic inhibitor thiabendazol and to caffeine, and died rapidly in stationary phase. Many of these phenotypes are similar to those of sep1 (kem1 or xrn1) mutants of S. cerevisiae. In contrast, the exo2 mutation had only a moderate effect on progression through meiosis and no significant effect on meiotic recombination. We discuss possible functions of the multifunctional ExoII protein.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Szankasi P,Smith GRdoi
10.1007/s002940050134subject
Has Abstractpub_date
1996-09-01 00:00:00pages
284-93issue
4eissn
0172-8083issn
1432-0983journal_volume
30pub_type
杂志文章相关文献
CURRENT GENETICS文献大全abstract::O-Mannosylation is suggested to be essential for protein secretion in Trichoderma reesei. In protein O-glycosylation, the first mannosyl residue is transferred to a serine or threonine hydroxyl group of the protein from dolichyl phosphate mannose by protein O-mannosyltransferase. In Saccharomyces cerevisiae, seven PMT...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-003-0368-5
更新日期:2003-04-01 00:00:00
abstract::In order to develop an eukaryotic vector with the Podospora plasmid, further characterization is required of the mitochondrial DNA into which this plasmid is integrated, a physical map (restriction sites) of the Podospora chondriome (size 95 kb) has been completed. As prerequisite for the establishment of a genetic (f...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00365705
更新日期:1982-07-01 00:00:00
abstract::When the beta 1-, beta 2- and beta 3-tubulin-specific DNAs from Drosophila melanogaster were used as probes to recognize tubulin-specific sequences in the chromosomes of Drosophila auraria, they were found to hybridize to the same polytene band in region 32C of the 2L polytene chromosome. Three overlapping clones were...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00712973
更新日期:1994-01-01 00:00:00
abstract::The yeast OMP decarboxylase gene has been cloned in the phage fd in both orientations to obtain pure DNA of each strand. With these probes the transcription of each strand of the yeast gene was measured in wild type yeast, either repressed or induced, in a yeast strain transformed by an hybrid plasmid containing the O...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00420621
更新日期:1980-10-01 00:00:00
abstract::We have found no evidence for an adaptive response for either lethality or mutagenesis following treatment of Saccharomyces cerevisiae with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). The rad6 and rad52 mutants of S. cerevisiae are highly defective in MNNG and ethyl methanesulfonate induced mutagenesis of both statio...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00410912
更新日期:1986-01-01 00:00:00
abstract::The gene for subunit II of cytochrome oxidase in the yeast Hansenula saturnus was previously shown to be located on a 1.7 kb HindIII-BamHI fragment of mitochondrial DNA (Lawson and Deters, accompanying paper). In this paper, we report the nucleotide sequence of a large part of this fragment, covering the coding region...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00421605
更新日期:1985-01-01 00:00:00
abstract::Mixed aeciospore isolates of Cronartium flaccidum and Peridermium pini were obtained from single-tree infections in Britain, Italy and Greece. The 5.8s ribosomal RNA gene and flanking intergenic transcribed spacer regions ITS1 and ITS2 were found to be highly similar between C. flaccidum and P. pini. Within samples he...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF02208620
更新日期:1996-03-01 00:00:00
abstract::The recovery of spontaneous canavanine-resistant mutants is reduced dramatically in a strain of Saccharomyces cerevisiae that carries a suppressed can 1-100 allele and is permeable to and auxotrophic for thymidylate. This effect does not occur in an isogenic strain that neither takes up nor requires the nucleotide. Ho...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00435459
更新日期:1989-02-01 00:00:00
abstract::ERG24 is the structural gene for the C14-sterol reductase in yeast. A lack of activity in that enzyme, mediated either by the morpholine fungicides or the insertional inactivation of ERG24, causes the accumulation of the aberrant sterol ignosterol. Cells producing this sterol are unable to grow aerobically in the rout...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050371
更新日期:1998-08-01 00:00:00
abstract::A vector was constructed for transformation of the plant pathogenic fungus Fusarium solani. The promoter 35Sp, from cauliflower mosaic virus, was fused to the bacterial gene APH(3')II, which confers resistance to the aminoglycoside antibiotic G418. Two transformation procedures were developed: one using isolated funga...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00376799
更新日期:1989-06-01 00:00:00
abstract::Fission yeast is a simple eukaryotic model organism in which many aspects of cell cycle control can be explored. We examined by homologous recombination whether the human CDC25A phosphatase could substitute for the function of the fission yeast Cdc25. We first show: (a). that CDC25A efficiently replaces the endogenous...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-003-0483-3
更新日期:2004-05-01 00:00:00
abstract::In the wood destroying fungus Schizophyllum commune, a well known subject for genetic studies, fruit bodies are produced not only in the course of the sexual cycle but also asexually. Sexual fruiting requires the establishment of a dikaryon which is under the control of the incompatibility factors A and B. Asexual fru...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00413309
更新日期:1979-12-01 00:00:00
abstract::In Chlamydomonas reinhardtii, the expression of the nit1 gene encoding nitrate reductase is dependent on the nature of the nitrogen source and on other environmental factors. We have fused the nit1 promoter region to the arylsulphatase (ars) reporter gene lacking its own promoter and introduced this chimeric construct...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050204
更新日期:1997-03-01 00:00:00
abstract::Ubiquitination/deubiquitination pathways are now recognized as key components of gene regulatory mechanisms in eukaryotes. The major transcriptional repressor for carbon catabolite repression in Aspergillus nidulans is CreA, and mutational analysis led to the suggestion that a regulatory ubiquitination/deubiquitinatio...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-016-0643-x
更新日期:2016-09-02 00:00:00
abstract::Previous work in other laboratories has shown that several mutagen sensitive mutants of Neurospora crassa are extremely sensitive to low levels of histidine in the culture medium. We have shown that wild type Neurospora accumulates nicks or breaks in the DNA in the presence of histidine. The number of nicks accumulati...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00365660
更新日期:1988-05-01 00:00:00
abstract::Two plasmids containing Saccharomyces cerevisiae 2 µm DNA sequences and the S. cerevisiae LEU2 gene have been found to display incompatibility with 2 µm DNA; in the presence of the LEU2 plasmids, 2 µm DNA can be lost. The LEU2 plasmids can be lost spontaneously after (and before) 2 µm DNA loss has occurred, so that st...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00435686
更新日期:1980-12-01 00:00:00
abstract::Sequence analysis of the rDNA region containing the internal transcribed spacer (ITS) regions and the 5.8s rDNA coding sequence was used to evaluate the genetic diversity of 45 isolates within and between anastomosis groups (AGs) in Rhizoctonia solani. The 5.8s rDNA sequence was completely conserved across all the AGs...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050272
更新日期:1997-09-01 00:00:00
abstract::To supply abundant ribosomes, multiple copies of ribosomal RNA genes (rDNA) are conserved from bacterial to human cells. In eukaryotic genomes, clusters of tandemly repeated rDNA units are present, and their number is stably maintained. Due to high level of transcription of rRNA genes, the repetitive structure is pron...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-019-00956-0
更新日期:2019-08-01 00:00:00
abstract::Chloroplast genes are typically organized into polycistronic transcription units that give rise to complex sets of mono- and oligo-cistronic overlapping RNAs through a series of processing steps. The psbB operon contains genes for the PSII (psbB, psbT, psbH) and cytochrome b(6)f (petB and petD) complexes which are nee...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050487
更新日期:1999-09-01 00:00:00
abstract::Uridine diphosphoglucose is not the sole donor for trehalose synthesis in yeast cells: an ADPG-dependent trehalose synthase, has been identified in mutant strains with undetectable UDPG-dependent trehalose-6-P synthase activity. Genetic and chromatographic studies indicate that the two activities correspond to differe...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00393399
更新日期:1989-08-01 00:00:00
abstract::Broad bean (Vicia faba) mitochondrial DNA (mtDNA) includes three circular plasmids: mt-plasmid 1 (1,704 ntp), mt-plasmid 2 (1,695 ntp) and mt-plasmid 3 (1,476 ntp). Partially replicated circular forms of these mt-plasmids have been observed in electron microscope preparations. Restriction enzymes that cleave either mt...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00569340
更新日期:1988-08-01 00:00:00
abstract::Loss of heterozygosity (LOH) in a vegetatively growing diploid cell signals irregularity of mitosis. Therefore, assays of LOH serve to discover pathways critical for proper replication and segregation of chromosomes. We screened for enhanced LOH in a whole-genome collection of diploid yeast strains in which a single g...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-018-0885-x
更新日期:2019-04-01 00:00:00
abstract::RAPD markers have been obtained in Cladosporium fulvum, in order to establish a genetic map based on mitotic recombination in this imperfect fungus. Segregation analysis has provided molecular evidence of a high degree of recombination during the parasexual cycle, and of the ploidy level of the parasexual progeny. A m...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00351783
更新日期:1994-05-01 00:00:00
abstract::La France disease of the cultivated mushroom, Agaricus bisporus, is known to be associated with the presence of a number of dsRNA segments. The nucleotide sequences of the dsRNAs M2 (1.3 kb), M1 (1.55 kb) and L3 (2.8 kb), invariably associated with the disease, were determined. Putative coding sequences for proteins w...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00312776
更新日期:1991-07-01 00:00:00
abstract::We have cloned and sequenced human and bovine cDNAs for the beta subunit of the ATP synthase (ATP-syn beta), a nuclear DNA (nDNA) encoded oxidative phosphorylation (OXPHOS) gene. The two cDNAs were found to share 99% amino acid homology and 94% nucleotide homology. The evolutionary rate of ATPsyn beta was then compare...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00434661
更新日期:1987-01-01 00:00:00
abstract::The cold-sensitive mutant strain of Neurospora crassa, crib-1 (PJ30201) has a conditional defect in ribosome biosynthesis. At 10 °C this mutant underproduces the small (37S) ribosomal subunit. Experiments were done to study rRNA synthesis in crib-1 after a shift from the permissive (25 °C) to the nonpermissive tempera...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00376778
更新日期:1981-09-01 00:00:00
abstract::We recently discovered the novel non-chromosomal determinant in Saccharomyces cerevisiae [NSI(+)] (nonsense suppression inducer), which causes omnipotent nonsense suppression in strains where the Sup35 N-terminal domain is deleted. [NSI(+)] possesses yeast prion features and does not correspond to previously identifie...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-011-0363-1
更新日期:2012-02-01 00:00:00
abstract::The Cys6Zn2 DNA-binding domain transcription factor Pdr1 is a central regulator of drug resistance in the pathogenic yeast Candida glabrata. In this review, I discuss the multiple control mechanisms modulating the function of this positive transcriptional regulator. Available data suggest that Pdr1 activity is restrai...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-018-0870-4
更新日期:2019-02-01 00:00:00
abstract::Seven MboI fragments spanning the mitochondrial apocytochrome b gene in Saccharomyces cerevisiae strain D273-10B were cloned in the BamHI site of the integrative yeast vector YIp5 and the capacity for autonomous replication was subsequently assayed in yeast. The positive correlation found between the ars-like activity...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00368060
更新日期:1987-01-01 00:00:00
abstract::In Volvox carteri, regA acts as a master gene to suppress all germ cell functions in somatic cells. Its product, RegA, has features of a transcriptional repressor. Here we report cDNA sequences representing 15 nuclear genes with properties expected of RegA targets: they are expressed strongly in germ cells and in regA...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050511
更新日期:1999-12-01 00:00:00