Abstract:
:We used ultrasound image-guided injections of high-titer retroviral vectors to obtain widespread introduction of genes into the mouse nervous system in utero as early as embryonic day 8.5 (E8.5). The vectors used included internal promoters that substantially improved proviral gene expression in the ventricular zone of the brain. To demonstrate the utility of this system, we extended our previous work in vitro by infecting the telencephalon in vivo as early as E8. 5 with a virus expressing Sonic Hedgehog. Infected embryos showed gross morphological brain defects, as well as ectopic expression of ventral telencephalic markers characteristic of either the medial or lateral ganglionic eminences.
journal_name
Nat Neuroscijournal_title
Nature neuroscienceauthors
Gaiano N,Kohtz JD,Turnbull DH,Fishell Gdoi
10.1038/12186subject
Has Abstractpub_date
1999-09-01 00:00:00pages
812-9issue
9eissn
1097-6256issn
1546-1726journal_volume
2pub_type
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