Abstract:
:We used antisense RNA to inhibit the expression of oncogene neu and investigated the effects of diminished neu expression on the phenotypes of B104 cells containing activated oncogene neu. Antisense MT-neu and pSV-neo plasmids were cotransfected into neuroblastoma B104 cells. Southern analysis showed the integration of anti-neu DNA into B104 cells. The expression of neu was inhibited up to 90% as quantitated by immunoprecipitation. The growth rate and the potential to differentiate in these transfectants were not affected as compared to the parental cell lines. The ability to grow in soft agar was inhibited more than 90% in these transfectants. Our results indicated that antisense-RNA against a specific oncogene can decrease the tumorigenicity of tumor cells but may not be able to revert it to normal cells completely.
journal_name
Cancer Lettjournal_title
Cancer lettersauthors
Shaw YT,Chang SH,Chiou ST,Chang WC,Lai MDdoi
10.1016/0304-3835(93)90028-8subject
Has Abstractpub_date
1993-04-15 00:00:00pages
27-32issue
1eissn
0304-3835issn
1872-7980pii
0304-3835(93)90028-8journal_volume
69pub_type
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