Abstract:
:Poly(A)-RNA prepared from the brains of 30-day-old male rats has been shown to direct the synthesis of neuron-specific enolase (NSE) in a cell-free system derived from rabbit reticulocytes. The newly synthesized polypeptides were immunoprecipitated and analyzed on SDS-polyacrylamide slab gels. Autoradiography indicated the synthesis of a product that comigrated with purified NSE and was recognized only by the anti-NSE antisera. Similar immunoprecipitation of reticulocyte lysates programmed with total RNA derived from embryonic chick heart failed to indicate the synthesis of NSE. These results show that the mRNA coding for NSE contains a poly(A) sequence and that brain-specific factors are not required for its translation.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Walker WA,Zomzely-Neurath Cdoi
10.1007/BF00964225subject
Has Abstractpub_date
1980-04-01 00:00:00pages
361-71issue
4eissn
0364-3190issn
1573-6903journal_volume
5pub_type
杂志文章abstract::Studies on primary astrocytes cultured in vitro have shown that process formation involves changes in cytoskeletal proteins and release of tension on the substratum. Actin filament reorganization has previously been found to be the major cytoskeletal change occurring during process formation. These changes are relativ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1022413718447
更新日期:1998-03-01 00:00:00
abstract::The release of the inhibitory neurotransmitter GABA is generally enhanced under potentially cell-damaging conditions. The properties and regulation of preloaded [3H]GABA release from mouse hippocampal slices were now studied in free radical-containing medium in a superfusion system. Free radical production was induced...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-007-9439-1
更新日期:2008-03-01 00:00:00
abstract::Protein kinase C has recently attracted considerable attention because of its importance in the control of cell division, cell differentiation, and signal transduction across the cell membrane. The activity of this enzyme is altered by several lipids such as diacylglycerol, free fatty acids, lipoxins, gangliosides, an...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/BF00973288
更新日期:1988-06-01 00:00:00
abstract::Human dental follicle cells (DFCs) are ectomesenchymal multipotent stem cells that form spheroid cell clusters (SCCs) under serum free medium cell culture conditions (SFM). Until today, molecular mechanisms for the formation of SCCs are unknown. In this study a quantitative phosphoproteomics approach revealed regulate...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-014-1298-y
更新日期:2014-07-01 00:00:00
abstract::In the experimental setting, taurine is known to be released from swollen cells to reestablish their normal volume. However, its clinical relevance has not been fully understood. This study was undertaken to reveal changes in cerebrospinal fluid (CSF) amino acids concentration in patients with severe traumatic brain i...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-004-9693-4
更新日期:2005-01-01 00:00:00
abstract::Three peptides overlapping the tryptophan region of bovine CNS myelin basic protein were synthesized by the solid phase procedure of Merrifield. These were the nonapeptide H-Phe-Ser-Trp-Gly-Ala-Glu-Gly-Gln-Lys-OH, the octapeptide H-Ser-Trp-Gly-Ala-Glu-Gly-Gln-Lys-OH, and the heptapeptide H-Trp-Gly-Ala-Glu-Gly-Gln-Lys-...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964458
更新日期:1980-01-01 00:00:00
abstract::Neuroprotective effects of metformin have been increasingly recognized in both diabetic and non-diabetic conditions. Thus far, no information has been available on the potential beneficial effects of metformin on peripheral nerve regeneration in diabetes mellitus. The present study was designed to investigate such a p...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-015-1803-y
更新日期:2016-05-01 00:00:00
abstract:UNLABELLED:Depressive disorder is a multifactorial diseases, that one of the typical feature are cognitive impairments. The aim of this study was to determine the total antioxidant status (TAS) in patients with recurrent depressive disorder (rDD) and to define relationship between plasma levels of TAS and the cognitive...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-012-0788-z
更新日期:2012-08-01 00:00:00
abstract::Rabbits sensitized with whole nervous tissue or myelin basic protein (MBP) plus adjuvant and developing experimental allergic encephalomyelitis (EAE) were studied for the presence of oligoclonal immunoglobulin (Ig) bands in spinal fluid and serum. Samples obtained prior to sensitization and at the time of sacrifice we...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00963909
更新日期:1981-01-01 00:00:00
abstract::The K-stimulated release of [3H]ACh from rat midbrain slices prelabeled by incubation with [3H]choline was dependent on extracellular Ca. Phenobarbital inhibited the K-stimulated [3H]ACh release and the IC50 was equal to that found for K-stimulated endogenous ACh release. These results support the suggestion that barb...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964710
更新日期:1980-07-01 00:00:00
abstract::Tamoxifen and toremifene are antiestrogenic drugs successfully used in the therapy of breast cancer. Rheumatoid arthritis and malaria have been treated with chloroquine for decades. Unfortunately, tamoxifen and chloroquine are reported to induce retinal changes as a side effect. We now studied the effects of tamoxifen...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/b:nere.0000010459.48656.4a
更新日期:2004-01-01 00:00:00
abstract::Ethylcholine mustard aziridinium ion (ECMA) was infused intracerebroventricularly (icv) to rats followed by measurement of two markers of presynaptic cholinergic neurons, choline acetyltransferase (ChAT) activity and high affinity choline transport (HAChT), in the hippocampus and cortex. Bilateral icv administration o...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00971589
更新日期:1988-08-01 00:00:00
abstract::The participation of voltage-sensitive Na+ channels (VSSC) on the changes on internal (i) Na+, K+, Ca2+, and on DA, Glu, and GABA release caused by different concentrations of 4-AP was investigated in striatum synaptosomes. TTX, which abolished the increase in Na(i) (as determined with SBFI), induced by 0.1 mM 4-AP on...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/b:nere.0000013737.17288.ce
更新日期:2004-02-01 00:00:00
abstract::Neuronal perikarya were isolated, using bulk preparative procedures, from bovine brains. Synaptosomes, neuronal perikarya, and brain homogenates had similar ganglioside patterns, with the synaptosomes containing at least four times more total ganglioside per mg protein than the neuronal perikarya and twice that of the...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00966108
更新日期:1976-04-01 00:00:00
abstract::Donepezil is a selective inhibitor of acetylcholinesterase (AChE) clinically used for treating Alzheimer's disease. Cholinergic effects after short-term exposure of donepezil (up to 12 h) have been extensively studied in rats, but few have addressed the potential long-term effects. After 14 days administration (1x3 mg...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-005-8828-6
更新日期:2005-12-01 00:00:00
abstract::Clinical studies have demonstrated that decreased adiponectin is associated with the development of Type 2 diabetes mellitus (T2DM) and Alzheimer's disease (AD). We focused on determining the neuroprotective effect offered by adiponectin against streptozotocin-induced brain damage in ICV-STZ rat model. We found that a...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-017-2426-2
更新日期:2018-02-01 00:00:00
abstract::Oxidative stress can induce neuronal apoptosis via the production of superoxide and hydroxyl radicals. This process is as a major pathogenic mechanism in neurodegenerative disorders. In this study, we aimed to clarify whether theaflavins protect PC12 cells from oxidative stress damage induced by H2O2. A cell model of ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-016-2069-8
更新日期:2016-12-01 00:00:00
abstract::Blue light impinging on the many mitochondria associated with retinal ganglion cells (RGCs) in situ has the potential of eliciting necroptosis through an action on RIP1/RIP3 to stimulate RGC death in diseases like glaucoma and diabetic retinopathy. Cells in culture die when exposed to blue light. The death process is ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-016-1946-5
更新日期:2016-09-01 00:00:00
abstract::Microglia can exacerbate central nervous system disorders, including stroke and chronic progressive neurodegenerative diseases such as Alzheimer disease. Mounting evidence points to ion channels expressed by microglia as contributing to these neuropathologies. The Chloride Intracellular Channel (CLIC) family represent...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-013-1084-2
更新日期:2013-09-01 00:00:00
abstract::The endogenous sialidase (N-acetylneuraminidase) activity of membranes prepared from goldfish retina and optic tectum displays characteristics similar to those reported for neural plasma membrane sialidases of other organisms. Endogenous membrane sialidase activity was found to be optimal at ph 4.0, and maximal releas...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965657
更新日期:1983-01-01 00:00:00
abstract::The effect of postdecapitative ischemia (5 min at 37 degrees) and hypoxia (5% O2, 95% N2, 30 min) on the distribution of protein radioactivity in the cellular fractions of guinea pig cerebral cortex was studied. Ischemic conditions resulted in the increase of total radioactivity level and protein content in the mitoch...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00963828
更新日期:1979-02-01 00:00:00
abstract::Rabbits were immunized with the myelin-associated glycoprotein (MAG) that had been purified from isolated rat brain myelin by selective extraction with lithium diiodosalicylate (LIS) and phenol followed by preparative SDS gel electrophoresis. Antibodies to MAG were detected qualitatively by immunodiffusion and quantit...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964417
更新日期:1981-10-01 00:00:00
abstract::Hyperprolinemia type II (HPII) is an autosomal recessive disorder caused by the severe deficiency of enzyme delta1-pyrroline-5-carboxylic acid dehydrogenase leading to tissue accumulation of proline. Chronic administration of Pro led to significant reduction of cytosolic ALT activity of olfactory lobes (50.57%), cereb...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-004-7026-2
更新日期:2004-12-01 00:00:00
abstract::Cloned fragments of members of the Drosophila and mouse major heat shock (hsp70) gene family were used to demonstrate that homologous sequences are present in the rabbit genome. After a physiologically relevant increase in body temperature of 3 degrees C, transcription of inducible hsp70 genes is detected in both the ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964846
更新日期:1985-09-01 00:00:00
abstract::Calpains, cathepsins and caspases play crucial role in mediating cell death. In the present study we observed a cascade of events involving the three proteases during middle cerebral artery occlusion (MCAo) in Wistar rats. The rats were MCA occluded and reperfused at various time points. We observed a maximal increase...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-007-9567-7
更新日期:2008-11-01 00:00:00
abstract::Crayfish glutamic acid decarboxylase (GAD), like the homologous enzymes from other species, is inhibited by carbonyl-trapping agents (e.g. aminooxyacetic acid; AOAA) and sulfhydryl reagents (e.g. 5,5'-dithiobis-(2-nitrobenzoic acid); DTNB). It also is inhibited by the product GABA, many anions (e.g. SCN- and Cl-), and...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964526
更新日期:1984-07-01 00:00:00
abstract::The effect of ageing and the relationships between the catalytic properties of enzymes linked to Krebs' cycle, electron transfer chain, glutamate and aminoacid metabolism of cerebral cortex, a functional area very sensitive to both age and ischemia, were studied on mitochondria of adult and aged rats, after complete i...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-009-0004-y
更新日期:2009-12-01 00:00:00
abstract::When excised goldfish spinal cord is kept in physiological saline at room temperature, the myelin multilayers swell. As studied by X-ray diffraction, the original repeating distance of 150 A (AS myelin) swells to 177 A (AL myelin); no intermediate distances are seen. At least 70% of the myelin can undergo this gradual...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965941
更新日期:1986-08-01 00:00:00
abstract::Pathophysiological concentrations of branched chain keto-acids (BCKAs), such as those that occur in maple syrup urine disease, inhibit oxygen consumption in liver homogenates and brain slices and the enzymatic activity of alpha-ketoglutarate- and pyruvate dehydrogenase complexes. Consistent with previous work, studies...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-007-9291-3
更新日期:2007-04-01 00:00:00
abstract::The frontal cortex, hippocampus, and cerebellum of the Fischer 344 rat were examined for an age-dependent change in neuronal and non-neuronal binding. Clonazepam and Ro5-6669 displaceable [3H]diazepam binding were used as indicators of [3H]diazepam binding on neuronal and non-neuronal membranes, respectively. In both ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964927
更新日期:1983-09-01 00:00:00