Abstract:
:Three peptides overlapping the tryptophan region of bovine CNS myelin basic protein were synthesized by the solid phase procedure of Merrifield. These were the nonapeptide H-Phe-Ser-Trp-Gly-Ala-Glu-Gly-Gln-Lys-OH, the octapeptide H-Ser-Trp-Gly-Ala-Glu-Gly-Gln-Lys-OH, and the heptapeptide H-Trp-Gly-Ala-Glu-Gly-Gln-Lys-OH. They were tested for encephalitogenic activity in guinea pigs with either Freund's complete adjuvant containing M. tuberculosis or muramyl dipeptide in incomplete Freund's adjuvant at doses of 10 microgram per animal. The results show that deletion of one or two residues from the amino-terminal end of the nonapeptide destroyed the ability of the shorter peptides to induce clinical but not histological signs of EAE.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Levit S,Powers JM,Milek D,Brostoff SWdoi
10.1007/BF00964458subject
Has Abstractpub_date
1980-01-01 00:00:00pages
37-42issue
1eissn
0364-3190issn
1573-6903journal_volume
5pub_type
杂志文章abstract::The effect of 4-aminopyridine on [3H]acetylcholine release was studied in rat cerebral cortical synaptosomes in the presence of a several secretagogues that have different mechanisms of action. As found previously, 4-aminopyridine increased [3H]acetylcholine release in a concentration-dependent manner (5-10 mM); a hig...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00969632
更新日期:1989-02-01 00:00:00
abstract::The exposure to hypobaric hypoxia increased lipid peroxidation as indicated by thiobarbituric acid-reactive substances [TBARS] in rat brain. Plasma lactate/pyruvate ratio was used as a marker of hypoxia. We compared the protective effect of alpha-tocopherol with the effect of L-carnitine or phosphocreatine. Rats pretr...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1020339530924
更新日期:2002-09-01 00:00:00
abstract::Transferrin (Tf), the iron-transport protein of vertebrate serum, is mainly synthesized in hepatocytes but is also found in other cell-types including oligodendrocytes. Our laboratory has characterized in a human oligodendrial cell line the presence of a new Tf transcript containing an alternative exon 1b replacing th...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1021692220482
更新日期:2002-11-01 00:00:00
abstract::This review presents data on metabotropic uridine nucleotide receptor subtypes (P2UR) activated by UTP, sometimes also by UDP and/or ATP. Some chemical details of receptor subtypes and ligand interactions are described. Ligand-activated P2UR subtypes may couple to different second messengers, yet little is known about...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1023/a:1022487128766
更新日期:1997-08-01 00:00:00
abstract::During the past decade a new approach to pathogenetic studies of hepatic encephalopathy has been undertaken to identify the neurochemical alterations which characterize the syndrome. Using animal models of hepatic encephalopathy electrophysiological, behavioral, pharmacological and biochemical evidence were provided o...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/BF00972205
更新日期:1990-02-01 00:00:00
abstract::Subunit-specific antibodies to all the gamma subunit isoforms described in mammalian brain (gamma(1), gamma(2S), gamma(2L), and gamma(3) have been made. The proportion of GABA(A) receptors containing each gamma subunit isoform in various brain regions has been determined by quantitative immunoprecipitation. In all tes...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF02529132
更新日期:1996-02-01 00:00:00
abstract::The aim of this study was to determine the anticancer potential of Leonurus sibiricus extract derived from in vitro transgenic roots transformed by Agrobacetrium rhizogenes with AtPAP1 transcriptional factor, and that of transformed roots without construct, on grade IV human glioma cells and the U87MG cell line, and a...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-018-2551-6
更新日期:2018-07-01 00:00:00
abstract::There is a growing body of evidence confirming the involvement of oxidative stress and inflammation in pathogenesis of schizophrenia. Inter-individual variation in antioxidant capacity caused by different genetic profile could potentially influence patient's susceptibility to oxidative damage. In this study we evaluat...
journal_title:Neurochemical research
pub_type: 杂志文章,随机对照试验
doi:10.1007/s11064-012-0937-4
更新日期:2013-02-01 00:00:00
abstract::Remyelination promoting human IgMs effectively increase the number of myelinated axons in animal models of multiple sclerosis. Hence, they ultimately stimulate myelin production by oligodendrocytes (OLs); however, their exact mechanism of action remains to be elucidated, and in particular, it remains unclear whether t...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-018-2701-x
更新日期:2019-06-01 00:00:00
abstract::The kinetics and specificity of taurine and beta-alanine uptake were studied in primary cultures of rat astrocytes under identical experimental conditions. The uptake consisted of nonsaturable penetration and saturable high-affinity transport that was strictly sodium dependent. The cells accumulated taurine more effec...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00967969
更新日期:1986-02-01 00:00:00
abstract::A previously described inhibitor of the UDP-N-acetylgalactosamine: GM3, N-acetylgalactosaminyltransferase (GalNAc-T) (Quiroga et al., 1, 2), was purified from chicken blood serum by a new procedure. When subjected to SDS-PAGE, two major polypeptides of 27 and 70 kDa were observed. When tested in vitro, only the 27 kDa...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1027320113151
更新日期:1997-04-01 00:00:00
abstract::It is known that the NMDA-R NR1 subunit is needed for the receptor activity and that under hypoxia the evolution toward apoptosis or neuronal survival depends on the balance NR2A/NR2B subunits. This paper analyzes the effect of acute hypoxia on the above mentioned subunits mRNAs during development. The mean percentage...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-010-0221-4
更新日期:2010-10-01 00:00:00
abstract::Altered proteostasis induced by amyloid peptide aggregation and hyperphosphorylation of tau protein, is a prominent feature of Alzheimer's disease, which highlights the occurrence of endoplasmic reticulum stress and triggers the activation of the unfolded protein response (UPR), a signaling pathway that enforces adapt...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-018-2469-z
更新日期:2018-03-01 00:00:00
abstract::In order to obtain further evidence of putative neurotransmitters in primary sensory neurons and interneurons in the dorsal spinal cord, we have studied the effects of unilateral section of dorsal roots and unilateral occlusion of the dorsal spinal artery on cholinergic enzyme activity and on selected amino acid level...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964851
更新日期:1989-05-01 00:00:00
abstract::The features of neuronal damage induced by the mitochondrial toxin NaN(3) were investigated in rat primary cortical neuron cultures. Cell viability (MTT colorimetric determination) and transmembrane mitochondrial potential (J-C1 fluorescence) were concentration-dependently reduced 24 h after NaN(3); neither nuclear fr...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-008-9852-0
更新日期:2009-05-01 00:00:00
abstract::The specific molecular events that underlie the age-related loss of cognitive function are poorly understood. Although not experimentally substantiated, age-dependent neuronal loss has long been considered central to age-related cognitive decline. More recently, age-related changes in brain white matter have taken pre...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-007-9341-x
更新日期:2007-12-01 00:00:00
abstract::The release of labeled norepinephrine from preloaded synaptosomes requires the presence of potassium and calcium. ATP-dependent binding of calcium to synaptic plasma membranes (SPM) may provide a means of maintaining the cation in a readily available pool for the triggering of transmitter release. A high Ca-binding ca...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965106
更新日期:1983-04-01 00:00:00
abstract::Ménière's disease (MD) is characterized by the triad of fluctuating hearing loss, episodic vertigo and tinnitus, and by endolymphatic hydrops found on post-mortem examination. Increasing evidence suggests that oxidative stress is involved in the development of endolymphatic hydrops and that cellular damage and apoptot...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-010-0304-2
更新日期:2010-12-01 00:00:00
abstract::Studies on primary astrocytes cultured in vitro have shown that process formation involves changes in cytoskeletal proteins and release of tension on the substratum. Actin filament reorganization has previously been found to be the major cytoskeletal change occurring during process formation. These changes are relativ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1022413718447
更新日期:1998-03-01 00:00:00
abstract::Evidence has suggested that cerebrospinal fluid-contacting nucleus (CSF-contacting nucleus) is correlated with the development and recurrence of pain. A recent research showed that the CSF-contacting nucleus acts as a component of the descending 5-hydroxytryptamine (5-HT) system and plays a role in descending pain inh...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-016-1880-6
更新日期:2016-07-01 00:00:00
abstract::Probenecid inhibits the transport of the small monocarboxylic acids lactate and propionate from blood to brain but does not affect the transport of 5HIAA or HVA. Neither lactate, 5HIAA, HVA, nor probenecid itself inhibits probenecid uptake into brain from blood and neither lactate nor 5HIAA itself inhibits 5HIAA uptak...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965898
更新日期:1982-10-01 00:00:00
abstract::Adaptive changes in serotonergic 5HT1 receptor signalling are believed to underlie the therapeutic effectiveness of antidepressant drugs. Since cells are continuously exposed to neurotransmitters/neuromodulators, spatially and temporally integrated, the responsiveness of a receptor system is dependent upon the physio-...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-007-9582-8
更新日期:2008-07-01 00:00:00
abstract::A case-control study was undertaken to investigate the status of platelet monoamine oxidase-B (MAO-B) activity in Indian cases of idiopathic Parkinson's disease. A significant increase in the activity of platelet MAO-B was observed in Parkinson's cases (n = 26) as compared to controls (n = 26). No significant change i...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-009-9929-4
更新日期:2009-08-01 00:00:00
abstract::N-Acetylaspartic and N-acetylaspartylglutamic acid concentrations in human ventricular, subarachnoid and lumbar cerebrospinal fluid were measured by combined gas chromatography-mass spectrometry using selected ion monitoring with deuterated internal standards. N-Acetylaspartate concentrations were in the range 55, 9, ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1020973023059
更新日期:1999-10-01 00:00:00
abstract::Our previous studies have shown that three sigma (sigma) receptor ligands, (+)-N-allylnormetazocine ((+)-SKF-10,047), (+/-)-pentazocine and 1,3-di(2-tolyl)guanidine (DTG) differently regulated the dopamine (DA) transmission in the rat brain. In the present study, we attempted to clarify the role of sigma 1 receptor su...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1027361101419
更新日期:1997-09-01 00:00:00
abstract::Neuronal excitation leads to an increase of the extracellular K(+) concentration ([K(+)]o) in brain. This increase has at least two energy-consuming consequences: (1) a depolarization-mediated change in intracellular pH (pHi) in astrocytes due to depolarization-mediated increased activity of the acid-extruding Na(+)/b...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-013-1149-2
更新日期:2013-11-01 00:00:00
abstract::SENP3 (SUMO-specific proteases 3), a member of the small ubiquitin-like modifier specific protease family, was identified as a molecule that deconjugates SUMOylation of modified protein substrates and functions as an isopeptidase by disrupting SUMO homeostasis to facilitate cancer development and progression. However,...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-012-0869-z
更新日期:2012-12-01 00:00:00
abstract::The current study investigated the neuroprotective activity of idebenone against pilocarpine-induced seizures and hippocampal injury in rats. Idebenone is a ubiquinone analog with antioxidant, and ATP replenishment effects. It is well tolerated and has low toxicity. Previous studies reported the protective effects of ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-014-1236-z
更新日期:2014-02-01 00:00:00
abstract::The effect of maternal hypoxia on the modification of the fetal brain cell membrane N-methyl-D-aspartate (NMDA) receptor and its modulatory sites was investigated. Experiments were conducted in pregnant guinea pigs of 60 days of gestation. Guinea pig fetuses were exposed to maternal hypoxia (FiO2 = 7%) for 60 minutes....
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00968402
更新日期:1992-12-01 00:00:00
abstract::We investigated the ability of hydrogen peroxide (H2O2) to cause apoptotic cell death in cultured rat forebrain neurons and the potential mechanisms by which oxidative stress triggers delayed neuronal death. H2O2 (25 microM for 5 min) reduced cell viability to 34.5 +/- 8.3% of untreated controls 20 h after exposure, a...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1022403224901
更新日期:1997-03-01 00:00:00