Abstract：
:The effect of maternal hypoxia on the modification of the fetal brain cell membrane N-methyl-D-aspartate (NMDA) receptor and its modulatory sites was investigated. Experiments were conducted in pregnant guinea pigs of 60 days of gestation. Guinea pig fetuses were exposed to maternal hypoxia (FiO2 = 7%) for 60 minutes. Tissue hypoxia in the fetal brain was documented biochemically by decreased levels of ATP and phosphocreatine (91.3% and 88.6% lower than normoxia, respectively). MK-801 binding characteristics (Bmax = number of receptors, Kd = affinity of receptor) were used as an index of NMDA receptor modification. P2 membrane fraction was prepared from the cortex of normoxic and hypoxic fetal brain and washed thoroughly before carrying out the binding assay. In hypoxic brains, Bmax decreased from the normoxic control level 0.79 +/- 0.03 pmol/mg protein to 0.58 +/- 0.03 pmol/mg protein (P < 0.005) and Kd value decreased (increased affinity) from 8.54 +/- 0.27 nM to 4.01 +/- 0.23 nM (P < 0.005) respectively. The MK-801 binding in the absence of added glutamate and glycine in hypoxic brain was 100% higher as compared to controls, indicating an increased sensitivity of the NMDA receptor to activation. The spermine dependent maximum activation of the NMDA receptor increased to 44% in the hypoxic animals as compared to 25% in controls. The Mg2+ response of the NMDA receptor was not affected by hypoxia.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Mishra OP,Delivoria-Papadopoulos Mdoi
10.1007/BF00968402keywords:
subject
Has Abstractpub_date
1992-12-01 00:00:00pages
1211-6issue
12eissn
0364-3190issn
1573-6903journal_volume
17pub_type
杂志文章abstract：:Berberine and palmatine exhibit a mild and competitive inhibition on bovine adrenal tyrosine hydroxylase (EC 1.14.16.2; TH). In this study, the inhibitory effects of protoberberine alkaloids (such as berberine, palmatine and coptisine) on dopamine biosynthesis in PC12 cells were investigated. Treatment with berberine ...
journal_title：Neurochemical research
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journal_title：Neurochemical research
pub_type： 杂志文章,评审
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pub_type： 杂志文章,评审
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pub_type： 杂志文章,撤回出版物
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更新日期：2020-09-01 00:00:00
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pub_type： 杂志文章
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更新日期：2020-07-01 00:00:00
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journal_title：Neurochemical research
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