Abstract：
:Comparative structure-function studies have been carried out for alpha-conotoxin GI acting on nicotinic acetylcholine receptors (AChR) from mammalian muscles and from the electric organ of the Torpedo californica ray and for alpha-conotoxin ImI, which targets the neuronal alpha7 AChR. A series of analogs has been prepared for this purpose: chemically modified derivatives, including a covalently linked dimer of GI, as well as analogs wherein one or several amino acid residues have been changed using solid-phase peptide synthesis. The activity of all compounds was assessed in competition with radioiodinated and/or tritiated alpha-conotoxin GI for binding to the membrane-bound AChR of Torpedo californica. Binding of radioiodinated alpha-conotoxin GI dimer was also monitored directly, revealing the largest, as compared to all other analogues, difference in the affinity between the two binding sites in the receptor (KD approximately 11 and 1200 nM). Comparison of binding data with the results of CD measurements point to important role of the spatial organization of the alpha-conotoxin second loop in manifestation of their "muscle" or "neuronal" specificity.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Kasheverov IE,Zhmak MN,Maslennikov IV,Utkin YN,Tsetlin VIdoi
10.1023/a:1022889827195keywords:
subject
Has Abstractpub_date
2003-04-01 00:00:00pages
599-606issue
3-4eissn
0364-3190issn
1573-6903journal_volume
28pub_type
杂志文章abstract：:In order to investigate the role of two free radical detoxificant enzymes in patients with aging brain disorders, superoxide dismutase (SOD) and catalase (CAT) activities have been measured in blood from male and female human patients of different ages with several types of aging brain disorders. When compared with ac...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF02532336
更新日期：1996-08-01 00:00:00
abstract：:Heme oxygenase-1 (HO-1) plays a crucial role in oxidative stress processes, apoptosis and cell differentiation. Further, some proteins related to cell cycle including cyclins and p21 are important markers of astrocyte cultures. Aim of investigation was to study the effects of cholinergic precursors (choline, CDP-choli...
journal_title：Neurochemical research
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doi：10.1007/s11064-012-0873-3
更新日期：2012-12-01 00:00:00
abstract：:Propofol and ketamine may provide certain degree of neuroprotection, but the underlying mechanism remains unclear to date. The cAMP response element-binding protein (CREB) was proposed that its phosphorylation at Ser133 (P-CREB) constituted a convergence point involved in neuroprotection. The purpose of this study was...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-011-0582-3
更新日期：2012-01-01 00:00:00
abstract：:More than a decade has passed since Zic family zinc finger proteins were discovered to be transcription factors controlling neuroectodermal differentiation (neural induction) in Xenopus laevis embryos. Although BMP-signal blocking has been shown to be a major upregulator of Zic genes in neuroectodermal differentiation...
journal_title：Neurochemical research
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abstract：:3-Nitropropionic acid (3-NP)-induced neurotoxicity can be used as a model for the genetic neurodegenerative disorder Huntington's disease (HD). A metabolic profiling strategy was adopted to explore the biochemical consequences of 3-NP administered to rats in specific brain regions. (1)H NMR spectroscopy was used to ch...
journal_title：Neurochemical research
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doi：10.1007/s11064-008-9904-5
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abstract：:N-terminal protein arginylation has been demonstrated in vitro and in situ and has been reported to increase following injury to sciatic nerves of rats. The present study attempts to demonstrate these reactions in vivo by applying [3H]Arg to the cut end of sciatic nerves in anesthetized rats and assaying for N-termina...
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journal_title：Neurochemical research
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abstract：:The subcellular distribution of Ca2+/calmodulin-stimulated adenylate cyclase activity was studied in comparison with that of guanine nucleotide-stimulated cyclase activity. The distributions of these activities were similar among the crude fractions but differed among the purified subsynaptosomal fractions. The specif...
journal_title：Neurochemical research
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journal_title：Neurochemical research
pub_type： 杂志文章,评审
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abstract：:The abnormal function of the voltage-gated potassium channel Kv10.2 can induce epilepsy. However, the physiological function of Kv10.2 in the central nervous system remains unclear. In this study, we found that Kv10.2 knockout (KO) increased the complexity of neurons in the CA3 subarea of hippocampus. Kv10.2 KO led to...
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更新日期：2020-12-01 00:00:00
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journal_title：Neurochemical research
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更新日期：2013-08-01 00:00:00
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journal_title：Neurochemical research
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doi：10.1023/a:1020902104056
更新日期：1999-03-01 00:00:00
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journal_title：Neurochemical research
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doi：10.1023/b:nere.0000023610.89631.d6
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abstract：:A role for protein phosphorylation in the process of neurite outgrowth has been inferred from many studies of the effects of protein kinase inhibitors and activators on cultured neurotumor cells and primary neuronal cells from developing brain or ganglia. Here we re-examine this issue, using a culture system derived f...
journal_title：Neurochemical research
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更新日期：1997-10-01 00:00:00
abstract：:6-Hydroxydopamine (6-OHDA) induces the production of reactive oxygen species (ROS) that are associated with various neurodegenerative diseases such as Parkinson's disease. 3,3',4',7-Tetrahydroxyflavone (fisetin), a plant flavonoid has a variety of physiological effects such as antioxidant activity. In this study, we i...
journal_title：Neurochemical research
pub_type： 杂志文章
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更新日期：2018-02-01 00:00:00
abstract：:Bilateral injection of 6-hydroxydopamine into the medial forebrain bundle (MFB) significantly decreased monoamine concentrations in the hypothalamus. The noradrenaline and serotonin content of the paraventricular nucleus (PVN) was also significantly reduced. These drastic decreases in neurotransmitter concentration di...
journal_title：Neurochemical research
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doi：10.1007/BF01474679
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journal_title：Neurochemical research
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abstract：:Diisopropyl phosphorofluoridate (DFP) produces organophosphorus ester-induced delayed neurotoxicity (OPIDN) in sensitive species. We have investigated the in vivo and in vitro effects of DFP on hen brain tubulin polymerization. Hens were treated with a single dose of DFP (1.7 mg/kg, sc.), and were sacrificed after 18-...
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journal_title：Neurochemical research
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abstract：:A hemagglutinating activity was detected in a synaptic vesicle-enriched fraction prepared from adult rat brain, using trypsinized glutaraldehyde-fixed rabbit erythrocytes. The specific activity of the fraction, in two series of experiments, was 7.5 and 16-fold higher than in the other subcellular fractions. The activi...
journal_title：Neurochemical research
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abstract：:BCL-2-associated athanogene-1(BAG-1) is a multifunctional and anti-apoptotic protein that was first identified as a binding partner of BCL-2. But the effects and mechanisms for BAG-1 against hypoxic damage is unclear up to now. Whether BAG-1 could protect the human brain against hypoxic damage through up-regulating 70...
journal_title：Neurochemical research
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更新日期：2017-10-01 00:00:00
