Abstract:
:BCL-2-associated athanogene-1(BAG-1) is a multifunctional and anti-apoptotic protein that was first identified as a binding partner of BCL-2. But the effects and mechanisms for BAG-1 against hypoxic damage is unclear up to now. Whether BAG-1 could protect the human brain against hypoxic damage through up-regulating 70 kDa heat shock proteins (HSP70) and PI3K/AKT pathway activation? In present study, we examined the changes of HSP70 and AKT and p-AKT protein level in SH-SY5Y cells with BAG-1L gene over-expression subjected to hypoxia/re-oxygenation injury. BAG-1L over-expression increased neuronal viability, and it reduced apoptosis of neurons after hypoxia/re-oxygenation for 8 h. BAG-1L over-expression enhanced the HSP70 protein levels and increased p-AKT/total AKT ratio after hypoxia/re-oxygenation for 8 h. These results suggest that BAG-1L over-expression protects against hypoxia/re-oxygenation injury, at least in part, by interacting with HSP70, and by accelerating the activation of PI3K/AKT pathways.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Wang Y,Jia C,Li QS,Xie CY,Zhang N,Qu Ydoi
10.1007/s11064-017-2304-ysubject
Has Abstractpub_date
2017-10-01 00:00:00pages
2861-2868issue
10eissn
0364-3190issn
1573-6903pii
10.1007/s11064-017-2304-yjournal_volume
42pub_type
杂志文章abstract::Discrete populations of anti-S49 antibodies were found in the antisera of Lewis rats recovered from S49-induced experimental allergic encephalomyelitis (EAE). A potent inducer of EAE in Lewis rats, S49 is a synthetic peptide representing residues 69-84 of bovine myelin basic protein but with deletions at Gly-77 and Hi...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00988601
更新日期:1985-12-01 00:00:00
abstract::We have adopted the PC12 cell line as in vitro cell model for studying Dp71 function in neuronal cells. These cells express a cytoplasmic (Dp71f) and a nuclear (Dp71d) isoform of Dp71 as well as various dystrophin-associated proteins (DAPs). In this study, we revealed by confocal microscopy analysis and Western blotti...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-009-0064-z
更新日期:2010-03-01 00:00:00
abstract::Microglia, the resident immune cells of the CNS, are primary regulators of the neuroimmune response to injury. Type I interferons (IFNs), including the IFNαs and IFNβ, are key cytokines in the innate immune system. Their activity is implicated in the regulation of microglial function both during development and in res...
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pub_type: 杂志文章,评审
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更新日期:2017-09-01 00:00:00
abstract::To assess the therapeutic effects of microRNA-21 (miR-21) knockdown (KD) for acute thoracic spinal cord contusion using a mouse model. Forty C57/BL6 mice were randomly divided into four groups: mice in the sham-operated (Sham) group received surgical procedure without spinal cord contusion; the spinal cord injury (SCI...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-018-2580-1
更新日期:2018-08-01 00:00:00
abstract::The effect of phenobarbital (100 mg/kg i.p.) and 6-aminonicotinamide (6AN) (35 mg/kg i.p.) on enzyme activities related to energy transduction was investigated on the homogenate "in toto", non-synaptic mitochondrial fraction and synaptosomal fraction isolated from different rat brain areas (cerebral cortex, hippocampu...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00971361
更新日期:1987-01-01 00:00:00
abstract::Rat obturator nerve 16S acetylcholinesterase (16S AChE) was separated by sucrose gradient velocity sedimentation and compared to the 16S form of AChE similarly derived from endplate regions of anterior gracilis muscles. The 16S AChE from both tissues could only be extracted in high ionic strength buffer; as it aggrega...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965031
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abstract::Chronic thiamine deprivation in the rat leads to ataxia, loss of righting reflex and neuropathological damage to lateral vestibular nucleus. Before onset of neurological symptoms, transketolase (TK) activities were found to be selectively reduced by 25% in lateral vestibular nucleus and surrounding pons. Further progr...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00972141
更新日期:1987-03-01 00:00:00
abstract::Oligodendrocytes (OL) are the only myelinating cells of the central nervous system thus interferences, either environmental or genetic, with their maturation or function have devastating consequences. Albeit so far neglected, one of the less appreciated, nevertheless possible, regulators of OL maturation and function ...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-019-02778-5
更新日期:2020-03-01 00:00:00
abstract::McGill-R-Thy1-APP rats express the human amyloid precursor protein carrying the Swedish and Indiana mutations. We examined the neurochemical content of the dorsal hippocampus in three-months-old male and female transgenic rats and healthy age- and gender-matched controls using in vivo (1)H MRS in order to assess early...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-013-1222-x
更新日期:2014-02-01 00:00:00
abstract::Ciliary ganglion neurons and nonneurons can be separated from each other, based on the greater adhesivity of the nonneurons to untreated tissue culture plastic in the presence of serum. When the separation was carried out in the presence of Serum Plus (a commercially available supplemented serum), two populations of n...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00966312
更新日期:1987-10-01 00:00:00
abstract::The chemical warfare nerve agent (CWNA) soman irreversibly inhibits acetylcholinesterase (AChE) causing seizure, neuropathology and neurobehavioral deficits. Pyridostigmine bromide (PB), the currently approved pretreatment for soman, is a reversible AChE inhibitor that does not cross the blood-brain barrier (BBB) to p...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-011-0564-5
更新日期:2011-12-01 00:00:00
abstract::In vitro synthesis of myelin proteolipid protein (PLP) was explored at different ages using rat brain total homogenates, incubated for 30 min with [3H]glycine. Total proteolipids, extracted from the incubated samples, were separated by SDS-PAGE and the radioactivity was measured in the band corresponding to myelin PLP...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00973044
更新日期:1984-09-01 00:00:00
abstract::Altered neurogenesis has been reported in Alzheimer disease (AD), the most common neurodegenerative disorder characterized with hyperphosphorylated tau and accumulation of β-amyloid (Aβ). Recent studies suggest that tau phosphorylation is essential for hippocampal neurogenesis, however, it is not known whether tau pho...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-010-0316-y
更新日期:2011-02-01 00:00:00
abstract::Three peptides overlapping the tryptophan region of bovine CNS myelin basic protein were synthesized by the solid phase procedure of Merrifield. These were the nonapeptide H-Phe-Ser-Trp-Gly-Ala-Glu-Gly-Gln-Lys-OH, the octapeptide H-Ser-Trp-Gly-Ala-Glu-Gly-Gln-Lys-OH, and the heptapeptide H-Trp-Gly-Ala-Glu-Gly-Gln-Lys-...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964458
更新日期:1980-01-01 00:00:00
abstract::The ability of Sendai virosomes or Lipofectin to introduce an AAV vector into primary rat brain astroglial cultures was characterized. The pJDT95npy vector was constructed by inserting rat NPY cDNA downstream from the indigenous AAV p5, p19 and p40 promoters in pJDT95. Lipofectin-mediated transfection with pJDT95npy (...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00967701
更新日期:1994-06-01 00:00:00
abstract::The serotoninergic system modulates nociceptive and locomotor spinal cord circuits. Exercise improves motor function and changes dopaminergic, noradrenergic, and serotonergic central systems. However, the direct relationship between serotonin, peripheral nerve lesion and aerobic treadmill exercise has not been studied...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-009-0066-x
更新日期:2010-03-01 00:00:00
abstract::Brief limb ischemia was reported to protect neurons against injury induced by subsequent cerebral ischemia-reperfusion, and this phenomenon is known as limb ischemic preconditioning (LIP). To explore the role of nitric oxide (NO) in neuroprotection of LIP in rats, we observed changes in the content of nitric oxide (NO...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-007-9381-2
更新日期:2007-11-01 00:00:00
abstract::Cell densities and respiratory rates of astrocytes from neonatal rat brain grown in primary culture were determined after 20-30 days in vitro. Cells grown in flasks reached lower densities (microgram DNA/cm2) and higher protein:DNA ratios than cells grown in petri dishes. Respiratory rates were lower for cells grown i...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964355
更新日期:1981-12-01 00:00:00
abstract::It is reported that chitinase1 increases in Alzheimer's disease (AD). However, the alteration of chitinase1 in the progress of AD is still unclear. Thus, we designed the present study to detect chitinase1 level in different stages of APP/PS1 double transgenic mice. Experimental models were APP/PS1 double transgenic mi...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-016-1874-4
更新日期:2016-07-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF01705532
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00973289
更新日期:1988-06-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1010917129951
更新日期:2001-05-01 00:00:00
abstract::Epilepsy is one of the oldest neurological conditions known to humankind. It is known that oxidative stress and generation of reactive oxygen species are a cause and consequence of epileptic seizures. Although recent years have seen tremendous progress in the molecular biology and metabolism of selenium, we still know...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-009-0015-8
更新日期:2009-12-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-004-6879-8
更新日期:2004-11-01 00:00:00
abstract::Glycogen is the main storage form of glucose in the brain. In contrast with previous beliefs, brain glycogen has recently been shown to play important roles in several brain functions. A fraction of metabolized glucose molecules are being shunted through glycogen before reentering the glycolytic pathway, a phenomenon ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-017-2267-z
更新日期:2017-09-01 00:00:00
abstract::S100beta is a soluble protein released by glial cells mainly under the activation of the 5-HT1A receptor. It has been reported as a neuro-trophic and -tropic factor that promotes neurite maturation and outgrowth during development. This protein also plays a role in axonal stability and the plasticity underlying long-t...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-009-9915-x
更新日期:2009-08-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1020984114824
更新日期:1999-01-01 00:00:00
abstract::Trigeminal neuralgia (TN) is a type of chronic neuropathic pain that is caused by peripheral nerve lesions that result from various conditions, including the compression of vessels, tumors and viral infections. MicroRNAs (miRs) are increasingly recognized as potential regulators of neuropathic pain. Previous evidence ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-019-02797-2
更新日期:2019-07-01 00:00:00
abstract::We have previously shown that extracellular ATP caused cell death in PC12 cells through activation of its receptors. Oxidative stress has been implicated as a mechanism of cell death caused by extracellular ATP. In the present study we examined the possible signal transduction cascades leading to cell death by extrace...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1022438703271
更新日期:1998-04-01 00:00:00
abstract::We report here the isolation of fractions enriched in components of the myelin-like membranes surrounding the giant axons of the earthworm. Lumbricus terrestris L. The composition and purity of the fractions have been assessed using SDS-protein electrophoresis, Western immunoblots, and electron microscopy. Preliminary...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00970759
更新日期:1988-09-01 00:00:00