Abstract：
:We have previously shown that extracellular ATP caused cell death in PC12 cells through activation of its receptors. Oxidative stress has been implicated as a mechanism of cell death caused by extracellular ATP. In the present study we examined the possible signal transduction cascades leading to cell death by extracellular ATP. We found, using the electrophoretic mobility shift assay, that transcription factor AP-1 DNA binding activity was stimulated by extracellular ATP. Northern blot analysis showed that mRNA levels of c-fos, c-jun were elevated after treatment with ATP. The stimulation was receptor mediated, since it was blocked by the ATP receptor antagonist, suramin. The stimulated AP-1 binding was also blocked by the antioxidant N-acetyl-L-cysteine, indicating that reactive oxygen species generated following ATP stimulation were involved in the induction of AP-1 activity. It appears that both translational and posttranslational events contributed to the increased AP-1 DNA binding since cyclohexamide (a protein synthesis inhibitor), genistein (tyrosine kinase inhibitor) and staurosporine (PKC inhibitor) each partially blocked the AP-1 activation. Changes in AP-1 DNA binding activity may modulate expression of target genes involved in cell death pathways.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Chen Y,Sun AYdoi
10.1023/a:1022438703271subject
Has Abstractpub_date
1998-04-01 00:00:00pages
543-50issue
4eissn
0364-3190issn
1573-6903journal_volume
23pub_type
杂志文章abstract：:The effect of hydrogen peroxide (H2O2) on cytosolic free calcium concentration ([Ca2+]c) as well as its effect on glutamate secretion in rat hippocampal astrocytes have been the aim of the present research. Our results show that 100 microM H2O2 induces an increase in [Ca2+]c, that remains at an elevated level while th...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-006-9078-y
更新日期：2006-06-01 00:00:00
abstract：:Activation of glial cells has been observed in neurodegenerative diseases including Alzheimer's disease (AD). Aggregation of amyloid β (Aβ) is profusely observed as characteristic pathology in AD brain. In our previous study using microglial cell line BV-2, tissue-type transglutaminase (TG2) was found to be involved i...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-017-2284-y
更新日期：2018-01-01 00:00:00
abstract：:Enolase is a glycolytic enzyme whose amino acid sequence is highly conserved across a wide range of animal species. In mammals, enolase is known to be a dimeric protein composed of distinct but closely related subunits: alpha (non-neuronal), beta (muscle-specific), and gamma (neuron-specific). However, little informat...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00970738
更新日期：1995-08-01 00:00:00
abstract：:1-Methyl-4-phenylpyridinium (MPP(+)) or 6-hydroxydopamine (6-OHDA) caused a nuclear damage, the mitochondrial membrane permeability changes, leading to the cytochrome c release and caspase-3 activation, the formation of reactive oxygen species and the depletion of GSH in PC12 cells. Nicardipine (a calcium channel bloc...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-006-9088-9
更新日期：2006-07-01 00:00:00
abstract：:The objective of the present study was to investigate the effects of preincubation of hippocampus homogenates in the presence of homocysteine or methionine on Na+, K+-ATPase and Mg2+-ATPase activities in synaptic membranes of rats. Homocysteine significantly inhibited Na+, K+-ATPase activity, whereas methionine had no...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1013907104585
更新日期：2001-11-01 00:00:00
abstract：:One hour incubation of rat cortical slices in a medium without oxygen and glucose (oxygen-glucose deprivation, OGD) increased S100B release to 6.53 ± 0.3 ng/ml/mg protein from its control value of 3.61 ± 0.2 ng/ml/mg protein. When these slices were then transferred to a medium containing oxygen and glucose (reoxygenat...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-014-1301-7
更新日期：2014-07-01 00:00:00
abstract：:During evolution, living organisms develop a specialized apparatus called nociceptors to sense their environment and avoid hazardous situations. Intense stimulation of high threshold C- and Adelta-fibers of nociceptive primary sensory neurons will elicit pain, which is acute and protective under normal conditions. A f...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-008-9711-z
更新日期：2008-10-01 00:00:00
abstract：:Primary malignant brain cancer, one of the most deadly diseases, has a high rate of recurrence after treatment. Studies in the past several years have led to the hypothesis that the root of the recurrence may be brain tumor stem cells (BTSCs), stem-like subpopulation of cells that are responsible for propagating the t...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-009-0079-5
更新日期：2009-12-01 00:00:00
abstract：:The activities of L-glutamate decarboxylase (GAD), GABA-transaminase (GABA-T), choline acetyltransferase (CAT), and cysteic and cysteinesulfinic acids decarboxylase (CAD/CSAD) in putamen and frontal cortex in both Huntington's chorea and normal tissues were measured. The greatest difference between Huntington's and no...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964435
更新日期：1979-10-01 00:00:00
abstract：:NMDA, the specific agonist of glutamate gated ion channels permeable to calcium, is implicated as a causal factor in the pathogenesis of several neurobiological disorders such as stroke, seizures, ischemia, and chronic neurodegenerative diseases. On the other hand, evidence on the roles of oxidative mechanisms involve...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF02533102
更新日期：1996-12-01 00:00:00
abstract：:We have synthesized the tertiary amines of pyridostigmine and neostigmine, 3-pyridinol dimethylcarbamate (norpyridostigmine) and 3-dimethylaminophenol dimethylcarbamate (norneostigmine) respectively, and we have tested their abilities to cross the blood-brain barrier and inhibit mouse brain AChE activity. The in vivo ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00973747
更新日期：1990-06-01 00:00:00
abstract：:The incidence of acute and chronic spinal cord injury (SCI) in the United States is more than 10,000 per year, resulting in 720 cases per million persons enduring permanent disability each year. The economic impact of SCI is estimated to be more than 4 billion dollars annually. Preclinical studies, case reports, and s...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-013-0991-6
更新日期：2013-05-01 00:00:00
abstract：:In order to develop a simplified method for long-term primary culture of highly-pure rat embryonic hippocampal neurons of low-density (10(3) cells/cm(2)), we optimized and modified conventional culturing methods. The modifications of our simplified method include: (1) combinational application of two growth substrates...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-010-0189-0
更新日期：2010-09-01 00:00:00
abstract：:Social isolation during early development is one of the most potent stressors that can cause alterations in the processes of brain maturation, leading to behavioral and neurochemical changes that may persist to adulthood. Exposure to palatable diets during development can also affect neural circuits with long-term con...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-013-1083-3
更新日期：2013-09-01 00:00:00
abstract：:Association of the candidate gene DLST with late-onset Alzheimer's disease (LOAD) risk has been suggested on the basis of case-control studies. This gene, located on chromosome 14q24.3, encodes a subunit of a mitochondrial component known to be defective in AD, the alpha-ketoglutarate dehydrogenase complex. Positive r...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/b:nere.0000014833.54481.1d
更新日期：2004-03-01 00:00:00
abstract：:Cholinesterase activities in rat forebrain, erythrocytes, and plasma were assessed after a single oral administration of metrifonate or dichlorvos. In 3-month-old rats, the dichlorvos (10 mg/kg p.o.)induced inhibition of cholinesterase reached its peak in brain after l5-45 min and after 10-30 min in erythrocytes and p...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF02531650
更新日期：1996-03-01 00:00:00
abstract：:Malformations of cortical development (MCD) are critical brain development disorders associated with varied abnormalities in both anatomic structures and neural functioning. It is also a very common etiology to the epilepsy, in which the alteration on excitability of cortical neurons is hypothesized as one of importan...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-016-1936-7
更新日期：2016-09-01 00:00:00
abstract：:The effects of neuroleptic treatments on dopamine transporters and on dopamine receptors was investigated in the forebrain of adult rats treated for 21 days with either haloperidol, clozapine or saline. The dopamine D1 receptors, labeled with [3H]SCH23390, increased in nucleus accumbens, latero-dorsal rostral neostria...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1022405621365
更新日期：1998-01-01 00:00:00
abstract：:Proteasome inhibition has been observed in many neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease. Here, the effect of proteasome inhibition on the morphology of cultured rat cortical astrocytes was investigated. Increasing evidence suggests that the function of astrocytes is related close...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-008-9830-6
更新日期：2009-05-01 00:00:00
abstract：:This article puts forward the hypothesis that the Low Density Lipid Receptor (LDLR) is one of the molecules that is involved in the clearance of amyloid proteins in the brain and that it may play a role in Alzheimer's Disease (AD) via its up-regulation by statins. The hypothesis is built on the following observations:...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-006-9084-0
更新日期：2006-06-01 00:00:00
abstract：:We have adopted the PC12 cell line as in vitro cell model for studying Dp71 function in neuronal cells. These cells express a cytoplasmic (Dp71f) and a nuclear (Dp71d) isoform of Dp71 as well as various dystrophin-associated proteins (DAPs). In this study, we revealed by confocal microscopy analysis and Western blotti...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-009-0064-z
更新日期：2010-03-01 00:00:00
abstract：:The goal of this study was to examine the responsiveness of an immortalized catecholaminergic neuronal line, 2N27, to various growth factors and identify those which promote catecholaminergic expression. 2N27 is a newly established neural cell line derived from fetal rat mesencephalic tissue and, thus, contains tyrosi...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1022482520358
更新日期：1997-03-01 00:00:00
abstract：:Endogenous and xenobiotic sulphur-containing convulsant and non-convulsant compounds containing structural moieties of, or bearing a structural resemblance to, GABA and homocysteine were tested in binding studies for their potency in displacing the GABA-mimetic [3H]muscimol from specific, high-affinity sites (Kd = 3.6...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00971006
更新日期：1987-07-01 00:00:00
abstract：:Radioactive azide derivatives of tetrodotoxin (TTX) were synthesized using 2-nitro-4-azidephenyl-[3H]beta alanine for the purpose of photolabeling of the Na channel. Three azide derivatives, N1, N2 and N3, were separated by ion exchange chromatography on Bio-Rex 70 resin and reversed phase high performance liquid chro...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965886
更新日期：1985-08-01 00:00:00
abstract：:The effect of postdecapitative ischemia (5 min at 37 degrees) and hypoxia (5% O2, 95% N2, 30 min) on the distribution of protein radioactivity in the cellular fractions of guinea pig cerebral cortex was studied. Ischemic conditions resulted in the increase of total radioactivity level and protein content in the mitoch...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00963828
更新日期：1979-02-01 00:00:00
abstract：:Postmortem changes in the activity of GDH, GAD, GABA-T, and GS in the adult forebrain and cerebellum and in the forebrain of newborns have been investigated. The activities of GDH, GAD, and GS were stable till 12 hr after death in both adult and young brain regions studied. The activity of GABA-T was stable till 30 mi...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966226
更新日期：1986-10-01 00:00:00
abstract：:The aim of this study is to investigate differences in thyroid-stimulating hormone (TSH) level in patients with acute schizophrenia, unipolar depression, bipolar depression and bipolar mania. Serum level of TSH was measured in 1,685 Caucasian patients (1,064 women, 63.1%; mean age 46.4). Mean serum TSH concentration w...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-014-1305-3
更新日期：2014-07-01 00:00:00
abstract：:Cerebral blood flow (CBF) and cerebellar blood flow (CeBF) were measured and correlated with brain lactate, pyruvate and adenosine triphosphate concentrations from groups representing 3-week old suckling (n = 10), 18-22-week old adult (n = 9) and 70-week old aged (n = 7) Sprague-Dawley rats before and during bilateral...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1027321203294
更新日期：1997-01-01 00:00:00
abstract：:Nicotinic agonists stimulate 22Na flux in rat pheochromocytoma PC12 cells. The stimulatory effect of carbamylcholine is maximal at 1 mM, while the stimulatory effect of nicotine and anatoxin maximize at the same level at 100 microM and 10 microM, respectively. The tertiary amines arecolone and isoarecolone have no eff...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965571
更新日期：1991-04-01 00:00:00
abstract：:Immunocytochemistry was used to determine sites of synthesis and pathways for the transport of the neuropeptide, Leucomyosuppressin (pQDVDHVFLRFamide) in the cockroach, Leucophaea maderae. This study led to identification of neurons in the brain and thoracic ganglia reactive to polyclonal antibodies raised against thi...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00974872
更新日期：1991-05-01 00:00:00
