Abstract:
:The objective of the present study was to investigate the effects of preincubation of hippocampus homogenates in the presence of homocysteine or methionine on Na+, K+-ATPase and Mg2+-ATPase activities in synaptic membranes of rats. Homocysteine significantly inhibited Na+, K+-ATPase activity, whereas methionine had no effect. Mg2+-ATPase activity was not altered by the metabolites. We also evaluated the effect of incubating glutathione, cysteine, dithiothreitol, trolox, superoxide dismutase and GM1 ganglioside alone or incubation with homocysteine on Na+, K+-ATPase activity. Tested compounds did not alter Na+, K+-ATPase and Mg2+-ATPase activities, but except for trolox, prevented the inhibitory effect of homocysteine on Na+, K+-ATPase activity. These results suggest that inhibition of this enzyme activity by homocysteine is possibly mediated by free radicals and may contribute to the neurological dysfunction found in homocystinuric patients.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Streck EL,Zugno AI,Tagliari B,Franzon R,Wannmacher CM,Wajner M,Wyse ATdoi
10.1023/a:1013907104585keywords:
subject
Has Abstractpub_date
2001-11-01 00:00:00pages
1195-200issue
11eissn
0364-3190issn
1573-6903journal_volume
26pub_type
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