Abstract:
:The incubation of neuronal nitric oxide synthase with the five amyloid peptide fragments [Aβ17-21; Aβ25-29; Aβ29-33; Aβ33-37; Aβ25-37] catalyzed the formation of fibrils. The role of neuronal isomer (nNOS) involved the entrapment of free monomers and seed aggregates to initiate the events of nucleation and elongation, critical for the formation of fibrils. It was evident that the hydrophobic nature of Aβ17-21, the three glycine zipper peptides [Aβ25-29; Aβ29-33; Aβ33-37] and Aβ25-37 was a trigger in the formation of fibrils and was a force critical in the association of the peptides with the enzyme. Gold and silver nanoparticles (average 4.0 nm) inhibited fibril formation when added to the induced fibrils from nNOS-Aβ incubation. The addition of nNOS and/or Aβ to co-incubated solutions of nanoparticle-Aβ or nanoparticle-nNOS respectively did not prevent fibril formation but reversed it. Three mechanisms for this reversal were proposed: (1) depletion of free Aβ monomer in solution and blocking potential aggregation sites on the nNOS molecule due to large surface area of the nanoparticle (2) hydrophobic interaction between the Aβ peptide and nanoparticle (3) disruption of binary adducts between Aβ-peptides and nNOS by nanoparticles.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Padayachee ER,Arowolo A,Whiteley CGdoi
10.1007/s11064-013-1206-xsubject
Has Abstractpub_date
2014-01-01 00:00:00pages
194-201issue
1eissn
0364-3190issn
1573-6903journal_volume
39pub_type
杂志文章abstract::Alzheimer's disease (AD) is a devastative neurodegenerative disorder with complex etiology. Apoptosis, a biological process that plays an essential role in normal physiology to oust a few cells and contribute to the normal growth, when impaired or influenced by various factors such as Bcl2, Bax, caspases, amyloid beta...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-014-1454-4
更新日期:2014-12-01 00:00:00
abstract::Several studies showed that the up-regulation of glial glutamate transporter-1 (GLT-1) participates in the acquisition of brain ischemic tolerance induced by cerebral ischemic preconditioning or ceftriaxone pretreatment in rats. To explore whether GLT-1 plays a role in the acquisition of brain ischemic tolerance induc...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-011-0639-3
更新日期:2012-03-01 00:00:00
abstract::C-6 glial cells were studied in culture with respect to morphological and biochemical changes under several experimental conditions. Doubling time was 33 hr for cells plated at either 0.5 or 1.0×10(6) cells per flask. Markedly reduced cell growth and astrocyte-like appearance were observed following dibutyryl cyclic A...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00966230
更新日期:1976-08-01 00:00:00
abstract::Omega-conotoxin GVIA (omega-CTX), as a selective blocker for an N-type Ca2+ channel, has been conveniently used in many molecular biochemical and pharmacological experiments. There has been little elucidation of 125I-omega-CTX binding sites (mainly the 135-kDa band) in the crude membranes from chick brain, although th...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1022805615926
更新日期:2003-05-01 00:00:00
abstract::Using sodium (NaN3)-induced anoxia plus aglycaemia as a model of chemically-induced ischemia, we have characterized the endogenous release of excitatory and inhibitory amino acids from superfused hippocampal slices. Chemical ischemia produced an azide (1-30 mM) dose-dependent increase in the efflux of glutamate, aspar...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1011094728469
更新日期:2001-02-01 00:00:00
abstract::The effect of isoproterenol, norepinephrine, dopamine, and serotonin on the accumulation of adenosine 3',5'-monophosphate (cAMP) was studied in homogeneous neuronal cultures from 8-day chick embryo hemispheres. Among the catecholamines, isoproterenol had a more pronounced effect on the accumulation of cAMP. Norepineph...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00966167
更新日期:1980-10-01 00:00:00
abstract::It is well-known that pH changes can influence a lot of cellular processes. In this work, we have specifically studied the influence of alkalinization, which can be developed in spinal cord neurons during hyperventilation (respiratory alkalosis) and chronic renal failure (metabolic alkalosis) on calcium homeostasis. A...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/b:nere.0000035800.65411.3e
更新日期:2004-09-01 00:00:00
abstract::Postmortem changes in the activity of GDH, GAD, GABA-T, and GS in the adult forebrain and cerebellum and in the forebrain of newborns have been investigated. The activities of GDH, GAD, and GS were stable till 12 hr after death in both adult and young brain regions studied. The activity of GABA-T was stable till 30 mi...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00966226
更新日期:1986-10-01 00:00:00
abstract::A cortical cup model with continuous perfusion of artificial cerebrospinal fluid (containing 134 mM NaCl) was used to investigate the effects of anion channel blockers on the hyposmotically-induced release of amino acids from the in vivo rat cerebral cortex. The hyposmotic stimulus (25 mM NaCl) evoked a release of tau...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1020902104056
更新日期:1999-03-01 00:00:00
abstract::Several epidemiological studies have shown that consumption of large quantities of vegetables especially cruciferous vegetables (Broccoli and Brussels sprouts) can protect against chronic diseases. Sulforaphane, an isothiocynate found in cruciferous vegetables has been demonstrated to have neuroprotective effects in s...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-016-1965-2
更新日期:2016-10-01 00:00:00
abstract::Sex differences exist in the functioning of the two brain corticosteroid receptor systems. Ovarian steroid replacement alters receptor mRNA expression, receptor binding capacities, and receptor affinity. The abundance of both mineralocorticoid receptor (MR) and glucocorticoid receptor (GR) message can be reduced by es...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1023/a:1022023207326
更新日期:1997-11-01 00:00:00
abstract::The overlapping distribution of opioid and cholecystokinin (CCK) peptides and their receptors (mu and delta opioid receptors; CCK-A and CCK-B receptors) in the central nervous system have led to a large number of studies aimed at clarifying the functional relationships between these two neuropeptides. Most of the phar...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/BF02532381
更新日期:1996-11-01 00:00:00
abstract::Previous studies have demonstrated that the c-Jun N-terminal kinase (JNK) pathway plays an important role in inducing neuronal apoptosis following cerebral ischemic injury. JNK signaling pathway in activated during cerebral ischemic injury. It participates in ischemia-induced neuronal apoptosis. However, whether JNK s...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-015-1753-4
更新日期:2016-04-01 00:00:00
abstract::A previous study conducted in this laboratory revealed a decrease in total cholinesterase (total ChE) in the cerebral cortex, hippocampus and striatum in aged rats (24 months) of various strains, as compared with young animals (3 months). The purpose of the present experiments was to extend the study to other brain ar...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00969013
更新日期:1992-08-01 00:00:00
abstract::An analytical method for the determination of saturated very long chain (VLC) fatty acids in the serum has been devised. Free fatty acids obtained after hydrolysis of total lipid extracts were converted into p-bromophenacyl esters. The derivatives were purified in two sequential steps by clean-up on C18 reversed-phase...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00968082
更新日期:1984-12-01 00:00:00
abstract::The characteristics and properties of the increase in cytosolic [Ca2+] that occurs in bovine adrenal medullary chromaffin cells on exposure to angiotensin 11 have been investigated. In fura-2 loaded cells exposure to a maximally effective concentration of angiotensin II (100 nM) caused a rapid, but transient increase ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1024987128090
更新日期:2003-09-01 00:00:00
abstract::The inflammatory response mediated by microglia in the central nervous system is closely related to epilepsy. Notch signaling plays an important role in the microglial activation during hypoxia. This study aimed to investigate whether Notch signaling is involved in microglial activation and subsequent inflammation-rel...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-018-2544-5
更新日期:2018-06-01 00:00:00
abstract::Oxidative stress is one of the most important pathological mechanisms in neurodegenerative diseases and ischemia. Recent studies have indicated that the sonic hedgehog (SHH) signaling pathway is involved in these diseases, but the underlying mechanisms remains elusive. Here we report that the SHH pathway was activated...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-010-0264-6
更新日期:2011-01-01 00:00:00
abstract::Because it appears that oxidative stress and inflammation are implicated with disease pathogenesis in the diabetic brain, many researchers have used streptozotocin (STZ)-induced diabetic animals to study superoxide production and the effects of superoxide scavengers like Cu,Zn-superoxide dismutase (SOD1). However, man...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-010-0280-6
更新日期:2011-01-01 00:00:00
abstract::The effect of ageing and the relationships between the catalytic properties of enzymes linked to Krebs' cycle, electron transfer chain, glutamate and aminoacid metabolism of cerebral cortex, a functional area very sensitive to both age and ischemia, were studied on mitochondria of adult and aged rats, after complete i...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-009-0004-y
更新日期:2009-12-01 00:00:00
abstract::Single prolonged stress (SPS) rats is a rodent model of post traumatic stress disorder (PTSD). Abnormal hippocampal morphology and function were found in the PTSD patients. Our previous study has shown that SPS induce loss of hippocampal neurons. But the effects of SPS on glial cells in the hippocampus have not been e...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-015-1549-6
更新日期:2015-05-01 00:00:00
abstract::Mammalian MTH1 protein, a MutT-related protein, catalyzes the hydrolysis of 8-oxo-7,8-dihydroguanosine triphosphate (8-oxoGTP) to monophosphate, thereby preventing incorporation of 8-oxo-7,8-dihydroguanine (8-oxoguanine) into RNA. In this study, we applied immunohistochemistry to follow the expression of MTH1 and the ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-011-0484-4
更新日期:2011-08-01 00:00:00
abstract::The present study has attempted to elucidate the alteration of serotonin turnover after 24 h REM sleep deprivation in different regions in brain of young rat. Sleep deprivation was induced by the inverted flower pot technique. Results of this study show increased serotonin turnover after 24 h REM sleep deprivation in ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-005-9139-7
更新日期:2006-01-01 00:00:00
abstract::The study aimed to directly measure in vivo superoxide radical (O*2) a direct indicator of oxidative stress, in the brain of rats with experimentally induced obstructive jaundice by employing a new quantitative ultrasensitive fluorescent assay requiring minimum sample. O*2 anion is specific for dihydroethidine (DHE) a...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-007-9556-x
更新日期:2008-06-01 00:00:00
abstract::Addiction is a debilitating, chronic psychiatric disorder that is difficult to cure completely owing to the high rate of relapse. Behavioral sensitization is considered to may underlie behavioral changes, such as relapse, caused by chronic abuse of psychomotor stimulants. Thus, its animal models have been widely used ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-016-1976-z
更新日期:2016-10-01 00:00:00
abstract::Lipid synthesis from acetoacetate and 3-hydroxybutyrate was studied in chick embryo from 15 to 21 days and in chick neonate from 1 to 21 days. Embryonic spinal cord showed higher ability than brain to incorporate acetoacetate into total lipids, although a sharp decrease was found at hatching. 3-Hydroxybutyrate incorpo...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00966692
更新日期:1993-10-01 00:00:00
abstract::CNTF (ciliary neurotrophic factor) has been suggested to be an important survival factor for oligodendrocytes; however, this effect is inconsistently obtained and myelination appears normal in CNTF null animals. On the other hand, CNTF stimulates astrocytes to produce growth and trophic factors. Therefore, we tested t...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-006-9151-6
更新日期:2007-02-01 00:00:00
abstract::The effect of optic nerve transsection on proteolytic degradation of axonally transported proteins in the superior colliculus of the rabbit was studied. Proteolysis of labeled proteins was determined in vitro in small pieces of the superior colliculus. Within 2 hours after sectioning the optic nerve there was a decrea...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00973280
更新日期:1988-07-01 00:00:00
abstract::Studies on primary astrocytes cultured in vitro have shown that process formation involves changes in cytoskeletal proteins and release of tension on the substratum. Actin filament reorganization has previously been found to be the major cytoskeletal change occurring during process formation. These changes are relativ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1022413718447
更新日期:1998-03-01 00:00:00
abstract::Several methods have been described previously for the purification of the nervous-tissue specific protein kinase C substrate B-50 (GAP-43). In this paper we present a new purification method for B-50 from rat brain which employs 2-mercaptoethanol to release the protein from isolated synaptosomal plasma membranes. Mos...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00998271
更新日期:1993-08-01 00:00:00