Abstract：
:During a variety of insults to the brain adenine nucleotides are released in large quantities from damaged cells, triggering multiple cellular responses to injury. Here, we evaluated changes in extracellular ATP, ADP and AMP hydrolysis at different times (0-24 hours) after unilateral cortical stab injury (CSI) in adult rats. Results demonstrated that 24 hours following CSI, ATP and ADP hydrolyzing activities were not significantly altered in injured cortex. Based on calculated V (ATP)/V (ADP) ratio it was concluded that ATP/ADP hydrolysis was primarily catalyzed by NTPDase1 enzyme form. In contrast, AMP hydrolysis, catalyzed by 5'-nucleotidase, was significantly reduced at least 4 hours following CSI. Kinetic analysis and Lineweaver-Burk transformation of the enzyme velocities obtained over the range of AMP concentrations (0.05-1.50 mM) revealed that inhibition of 5'-nucleotidase activity after CSI was of the uncompetitive type. Taken together our data suggest that injured tissue has reduced potential for extracellular metabolism of adenine nucleotides in early stages after CSI.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Nedeljkovic N,Bjelobaba I,Lavrnja I,Stojkov D,Pekovic S,Rakic L,Stojiljkovic Mdoi
10.1007/s11064-007-9529-0subject
Has Abstractpub_date
2008-05-01 00:00:00pages
873-9issue
5eissn
0364-3190issn
1573-6903journal_volume
33pub_type
杂志文章abstract：:Histidinemia is an inherited metabolic disorder biochemically characterized by high concentrations of histidine in biological fluids. Usually affected patients are asymptomatic although some individuals have mental retardation and speech disorders. Considering the high prevalence of histidinemia and the scarce informa...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-012-0703-7
更新日期：2012-05-01 00:00:00
abstract：:In this study, we observed the effects of metformin, one of the most widely prescribed drugs for the treatment of type 2 diabetes, on cell proliferation and neuroblast differentiation in the subgranular zone of the hippocampal dentate gyrus (SZDG) in Zucker diabetic fatty (ZDF) rats, which are a model for type 2 diabe...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-009-0115-5
更新日期：2010-04-01 00:00:00
abstract：:The maximal rate of some cerebral enzymatic activities related to energy transduction (hexokinase; phosphofructokinase; lactate dehydrogenase; citrate synthase; malate dehydrogenase; total NADH-cytochrome c reductase; cytochrome oxidase), amino acid metabolism (glutamate decarboxylase; glutamate dehydrogenase) and cho...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00963926
更新日期：1983-02-01 00:00:00
abstract：:α-Synuclein (α-Syn) plays a crucial role in the pathophysiology of Parkinson's disease (PD), the degeneration of dopaminergic neurons. Previous studies have shown that α-Syn regulates dopamine synthesis by binding to and inhibiting tyrosine hydroxylase (TH). In neurons, protein phosphatases (PPs) play a prominent role...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-014-1477-x
更新日期：2015-03-01 00:00:00
abstract：:Human amniotic membrane mesenchymal stem cells (hAMSCs) are considered ideal candidate stem cells for cell-based therapy. In this study, we assessed whether hAMSCs transplantation promotes neurological functional recovery in rats after traumatic spinal cord injury (SCI). In addition, the potential mechanisms underlyin...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-016-1987-9
更新日期：2016-10-01 00:00:00
abstract：:The Fatty Acid method was used to determine whether incorporation of plasma radiolabeled arachidonic acid into brain phospholipids is controlled by phospholipase A2. Awake rats received an i.v. injection of a phospholipase A2 inhibitor, manoalide (10 mg/kg), and then were infused i.v. with [1-(14)C]arachidonate or [3H...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1020788031720
更新日期：1998-10-01 00:00:00
abstract：:In the present paper we describe the presence in avian CNS of an endogenous inhibitor of [3H]flunitrazepam binding. This compound was extracted from a synaptic membrane fraction isolated from chick optic lobe and brain using an exhaustive aqueous washing procedure, then purified by means of solid-phase extraction with...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1020702823600
更新日期：1998-11-01 00:00:00
abstract：:Using sodium (NaN3)-induced anoxia plus aglycaemia as a model of chemically-induced ischemia, we have characterized the endogenous release of excitatory and inhibitory amino acids from superfused hippocampal slices. Chemical ischemia produced an azide (1-30 mM) dose-dependent increase in the efflux of glutamate, aspar...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1011094728469
更新日期：2001-02-01 00:00:00
abstract：:Previous studies have shown that hypoxia induces nitric oxide synthase-mediated generation of nitric oxide free radicals leading to peroxynitrite production. The present study tests the hypothesis that hypoxia results in NO-mediated modification of Na+, K+-ATPase in the fetal brain. Studies were conducted in guinea pi...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1012331108641
更新日期：2001-10-01 00:00:00
abstract：:The effects of palmitate on intracellular and extracellular amino acid concentrations of cultured astrocytes was studied. Exposure of astrocytes to either 0.72 mM or 0.36 mM palmitate was associated with a significant reduction in the intracellular pool of glutamine and taurine. In contrast, the intracellular concentr...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF01000040
更新日期：1989-04-01 00:00:00
abstract：:Complementary DNA (cDNA) clones containing the entire coding sequence for Diazepam Binding Inhibitor (DBI) peptide, a 10-kDa precursor of putative natural ligands of benzodiazepine recognition sites, were isolated from rat, human and cow libraries. The sequence of all these clones is highly conserved; however, the N-t...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/BF00972202
更新日期：1990-02-01 00:00:00
abstract：:In the present study, we aimed to investigate the effects of pinealectomy and chronic melatonin administration on focal epileptiform activity induced by penicillin in the rat cortex and to determine the relation between melatonin levels and electrocorticogram (ECoG) power spectrum. For this purpose, male Sprague-Dawle...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-013-1069-1
更新日期：2013-08-01 00:00:00
abstract：:The effect of oxidative stress, induced by Fe(2+)-EDTA system, on Na+,K(+)-ATPase, Na+/CA2+ exchanger and membrane fluidity of synaptosomes was investigated. Synaptosomes isolated from gerbil whole forebrain were incubated in the presence of 200 microM FeSO4-EDTA per mg of protein at 37 degrees C for 30 min. The oxida...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1021918931780
更新日期：1997-12-01 00:00:00
abstract：:Oxidative stress is a long-hypothesized cause of diverse neurological and psychiatric disorders but the pathways by which physiological redox perturbations may detour healthy brain development and aging are unknown. We reported recently (Foley et al., Neurochem Res 39:2030-2039, 2014) that two-electron oxidations, to ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-016-1991-0
更新日期：2016-10-01 00:00:00
abstract：:Repeated electroconvulsive shock is an effective treatment for affective disorders. Striatum, hippocampus and brainstem are involved in affective disorders. Sodium-potassium/ATPase is of paramount importance for the proper functioning of the brain and its involvement in the affective disorders has been claimed for a l...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-006-9046-6
更新日期：2006-04-01 00:00:00
abstract：:The present study examined the potential membrane retention of desipramine (DMI) following exposures of 293-hNET cells to DMI, and its effect on [3H]NE uptake. Incubation of cells with 500 nM DMI for 1 h or 1 day persistently inhibited the uptake of [3H]NE up to 7 days, despite daily repeated washing of cells with dru...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/b:nere.0000013747.04964.46
更新日期：2004-02-01 00:00:00
abstract：:Glutamate and its receptors are expressed very early during development and may play important roles in neurogenesis, synapse formation and brain wiring. The levels of glutamate and activity of its receptors can be influenced by exogenous factors, leading to neurodevelopmental disorders. To investigate the role of NMD...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-006-9213-9
更新日期：2007-02-01 00:00:00
abstract：:ESR spin trapping technique was used to detect and analyze free radical formation. When 6-hydroxydopamine (6-OHDA) was incubated alone or in the presence of a free radical generating system (H2O2 and FeSO4), hydroxyl free radicals were observed in a concentration-dependent manner. Glutathione was found to be the most ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965833
更新日期：1991-01-01 00:00:00
abstract：:We previously reported that vanilloid receptor type 1 (VR1, or TRPV1) was up-regulated in dorsal root ganglion (DRG) and the spinal dorsal horn after chronic inflammatory pain produced by complete Freund's adjuvant (CFA) injection into the plantar of rat hind paw. In the present study, we found that subcutaneous or in...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-008-9751-4
更新日期：2008-10-01 00:00:00
abstract：:The central control of growth hormone (GH) secretion from the pituitary gland is ultimately achieved by the interaction between two hypothalamic neurohormones, somatostatin which inhibits and growth hormone-releasing hormone (GHRH) which stimulates GH release. The regulation of the somatostatin and GHRH release from t...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-005-9017-3
更新日期：2006-02-01 00:00:00
abstract：:Adult neural stem cells can be isolated from various regions of the rat brain and seem to have multilineage differentiation potential. In this study, we investigated the hypothesis that global protein expression of adult neural stem cells isolated from rat hippocampus is changed during in vitro differentiation. After ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/b:nere.0000023600.25994.11
更新日期：2004-06-01 00:00:00
abstract：:Exposure to sevoflurane and other inhalational anesthetics can induce cognitive impairment in elderly patients. Studies have indicated that methylene blue (MB) has beneficial effects on multiple neurodegenerative diseases and the mechanism involves mitochondrial function preservation. However, whether MB can attenuate...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-020-02976-6
更新日期：2020-04-01 00:00:00
abstract：:Autoantibodies to three eukaryotic 60S ribosomal phosphoproteins P0, P1 and P2 have been found in the sera of 10-20% of patients with systemic lupus erythematosus (SLE). These antibodies inhibit protein synthesis in vitro and when microinjected into cultured human fibroblasts. The three proteins share a common epitope...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00969925
更新日期：1990-04-01 00:00:00
abstract：:Rat tissues were tested for their ability to inhibit the binding of [3H]dihydromorphine or [3H]naloxone to membrane-bound opiate receptors. By this criterion, morphine-like substances were found in lung, heart, liver, and kidney as well as in brain. The relative activity of the extracts, based on initial tissue weight...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00963809
更新日期：1979-06-01 00:00:00
abstract：:This article puts forward the hypothesis that the Low Density Lipid Receptor (LDLR) is one of the molecules that is involved in the clearance of amyloid proteins in the brain and that it may play a role in Alzheimer's Disease (AD) via its up-regulation by statins. The hypothesis is built on the following observations:...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-006-9084-0
更新日期：2006-06-01 00:00:00
abstract：:The effects of vinpocetine on internal Na+ (Na(i)), cAMP accumulation, internal Ca2+ (Ca(i)) and excitatory amino acid neurotransmitters release, under resting and under depolarized conditions, was investigated in rat striatum synaptosomes. Veratridine (20 microM) or high K+ (30 mM) were used as depolarizing agents. R...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1021164418478
更新日期：1999-12-01 00:00:00
abstract：:It has been reported that electrical stimulation (ES) can promote nerve regeneration. One of the key factors leading to the promotion is calcium, which plays a vital role in the regulation on electrical activity of neurons, but the detailed mechanism is still an open question. In this study, ES was used to stimulate c...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-013-1197-7
更新日期：2014-01-01 00:00:00
abstract：:2',3'-cyclic nucleotide 3'-phosphohydrolase (CNP) was phosphorylated in vivo, in brain slices and in a cell free system. Phosphoamino acid analysis of immunoprecipitated CNP labeled in vivo and in brain slices revealed phosphorylation of phosphoserine (94%) and phosphothreonine (5%) residues. Phosphorylation of CNP in...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00967712
更新日期：1994-06-01 00:00:00
abstract：:The concentrations of taurine in a number of brain regions and in other tissues of rhesus monkeys fed a taurine-free human infant formula for 3 months are substantially lower than in similar monkeys fed the same formula supplemented with taurine. Activities of enzymes involved in taurine biosynthesis were not differen...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00972479
更新日期：1988-04-01 00:00:00
abstract：:Ethyl pyruvate (EP) has recently been reported to afford protection against neurodegenerative disorders. However, the mechanism underlying EP-mediated neuroprotection remains to be elucidated. Because peroxynitrite has been extensively implicated in the pathogenesis of various forms of neurodegenerative disorders via ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-009-0059-9
更新日期：2010-02-01 00:00:00
