Abstract:
:Cerebral deposition of amyloid β-peptide (Aβ), a fundamental feature of Alzheimer's disease (AD), damages the neurocytes and impairs the cognition functions and associative learning memory of AD patients. A series of novel 2-arylethenylquinoline derivatives were synthesized and evaluated in our previous study, which inhibited Aβ aggregation in vitro effectively at the concentration of 20 μmol/L and exhibited high antioxidant activity. In order to verify the capacity of anti-AD in vivo, the transgenic Caenorhabditis elegans (C. elegans) strain CL2355 expressing neural Aβ was employed as the AD model to investigate the neuroprotective activity of seven high-potential compounds (4a1, 4a2, 4b1, 4b2, 4c1, 4c2, 4c3) selected from those derivatives. Learning memory associated chemotaxis assay was performed to evaluate the neural repairment capacity. The underlying mechanism was investigated by mRNA analysis of Aβ gene and heat shock protein genes (hsp-16.1 and hsp-16.2) and Western blot of Aβ. Our data indicated that among seven tested compound, 4b1 and 4c2 reduced Aβ-induced stress, suppressed the expression of neural Aβ monomers and toxic oligomers, and recovered the damaged associative learning memory in C. elegans AD model. These findings further confirmed their potentials to become valuable agents for AD therapy.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
He Q,Huang G,Chen Y,Wang X,Huang Z,Chen Zdoi
10.1007/s11064-017-2339-0subject
Has Abstractpub_date
2017-11-01 00:00:00pages
3061-3072issue
11eissn
0364-3190issn
1573-6903pii
10.1007/s11064-017-2339-0journal_volume
42pub_type
杂志文章abstract::The uptake of radioactive ethanolamine has been studied in exclusively neuronal and glial cell cultures from dissociated cerebral hemispheres of chick embryos. Both cell types show saturable kinetics; neurons have an apparent Km of 6.7 microM, Vmax 41.4 pmol mg prot.-1 min-1 and glial cells a Km of 119.6 microM, Vmax ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965162
更新日期:1986-01-01 00:00:00
abstract::This study aimed at comparing the binding characteristics of [3H]ketanserin, a high-affinity serotonin 2A (5-HT2A) receptor antagonist, in the prefrontal cortex, hippocampus and striatum of human brain post-mortem. The results indicated the presence of a single population of binding sites in all the regions investigat...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1027366413289
更新日期:1997-06-01 00:00:00
abstract::The standard ELISA technique was improved for the detection of antigalactocerebroside antibody in biological fluid. Mouse monoclonal antigalactocerebroside antibody was used to demonstrate specificity and sensitivity of the technique. After optimization of the assay, the usefulness of this measurement for the evaluati...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00971533
更新日期:1988-03-01 00:00:00
abstract::The present study examined the potential membrane retention of desipramine (DMI) following exposures of 293-hNET cells to DMI, and its effect on [3H]NE uptake. Incubation of cells with 500 nM DMI for 1 h or 1 day persistently inhibited the uptake of [3H]NE up to 7 days, despite daily repeated washing of cells with dru...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/b:nere.0000013747.04964.46
更新日期:2004-02-01 00:00:00
abstract::Apoptosis is a highly complex form of cell death that can be triggered by alterations in Ca(2+) homeostasis. Members of the Bcl-2 family may regulate apoptosis and modulate Ca(2+) distribution within intracellular compartments. Bax, a proapoptotic member of the family, is constitutively expressed and soluble in the cy...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-011-0411-8
更新日期:2011-05-01 00:00:00
abstract::This study evaluates whether physiological variables differentially affect the local synthesis of protein constituents of synapses in subcellular fractions containing pinched-off dendrites (synaptodendrosomes). Synaptodendrosomes were pulse-labeled in a medium containing 35S-methionine with 3 or 25 mM KCl and in the p...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF02527725
更新日期:1996-06-01 00:00:00
abstract::Axonal regeneration across the site of spinal cord lesion is often aborted in adult mammalian species. The use of DNA vaccine to nullify the inhibitory molecules has been shown to be effective in promoting axonal regeneration in injured spinal cord. The possible molecular mechanisms, however, remain to be elucidated. ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-009-9951-6
更新日期:2009-09-01 00:00:00
abstract::Vascular endothelial growth factor (VEGF) is a promising biological marker and prognostic indicator in many neurological diseases. Although VEGF concentrations in plasma and cerebrospinal fluid (CSF) are increasingly reported, CSF-VEGF stability pre- and during-assay procedures is seldom evaluated. In the current stud...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-011-0517-z
更新日期:2011-11-01 00:00:00
abstract::Acupuncture and moxibustion are traditional medical treatments that have come to play important roles in complementary and alternative medicines. Moxibustion also has a long history as a folk remedy in Japan, particularly due to the technical simplicity and selective efficacy on certain types of disease and distress. ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-005-8839-3
更新日期:2005-12-01 00:00:00
abstract::To evaluate the influence of light and darkness on brain pyroglutamyl-peptidase I (pGluPI) activity, four experimental groups of rats were compared at the same time-point (10.00 h). Two groups were designed with a standard 12-12 h light-dark cycle: In group A, the lights were on from 7.00 h to 19.00 h, and the experim...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1010996425408
更新日期:2001-05-01 00:00:00
abstract::Chemotherapy drugs such as vincristine (VCR) can cause neuropathic pain, and there is still lack of ideal strategy to treat it. The current study was designed to investigate effect of matrine (MT) on VCR-induced neuropathic pain in animal model. VCR (75 μg/kg, i.p. for 10 consecutive days) was administered to induce p...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-016-2040-8
更新日期:2016-11-01 00:00:00
abstract::Neurons, purified by bulk-isolation procedures from 10 day-old rat brain, are 80-90% homogeneous. Contaminants are primarily blood vessels and occasional oligodendroglia. All sizes and shapes of neurons are obtained, as they are isolated from all areas of the brain, except the cerebellum. Neurotransmitter enzyme activ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00971368
更新日期:1987-01-01 00:00:00
abstract::Rat tissues were tested for their ability to inhibit the binding of [3H]dihydromorphine or [3H]naloxone to membrane-bound opiate receptors. By this criterion, morphine-like substances were found in lung, heart, liver, and kidney as well as in brain. The relative activity of the extracts, based on initial tissue weight...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00963809
更新日期:1979-06-01 00:00:00
abstract::Numerous studies have demonstrated that the Acetyl Coenzyme A-dependent arylamine NAT enzyme exist in many tissues of experimental animals including humans, and that NAT has been shown to be exist in mouse brain tissue. Increased NAT activity levels are associated with increased sensitivity to the mutagenic effects of...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1007632326953
更新日期:2000-11-01 00:00:00
abstract::Cloned fragments of members of the Drosophila and mouse major heat shock (hsp70) gene family were used to demonstrate that homologous sequences are present in the rabbit genome. After a physiologically relevant increase in body temperature of 3 degrees C, transcription of inducible hsp70 genes is detected in both the ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964846
更新日期:1985-09-01 00:00:00
abstract::The composition of retrogradely transported axonal proteins was examined by acrylamide gel electrophoresis and gel autoradiography in the experimental neuropathy induced in rats by p-bromophenylacetylurea (BPAU). Protein composition was normal during the early phase of retrograde transport but showed significant abnor...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00966781
更新日期:1993-06-01 00:00:00
abstract::Diminished thiamine (vitamin B1) dependent processes and oxidative stress accompany Alzheimer's disease (AD). Thiamine deficiency in animals leads to oxidative stress. These observations suggest that thiamin may act as an antioxidant. The current experiments first tested directly whether thiamin could act as an antiox...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-010-0242-z
更新日期:2010-12-01 00:00:00
abstract::Apoptotic cell death pathways have been implicated in acute brain injuries, including cerebral ischemia, brain trauma, and spinal cord injury, and in chronic neurodegenerative diseases. Experimental ischemia and reperfusion models, such as transient focal/global ischemia in rodents, have been thoroughly studied and su...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-004-6869-x
更新日期:2004-11-01 00:00:00
abstract::The concentrations of noradrenaline (NA), dopamine (DA), serotonin (5-HT), and their metabolites were determined in 5 brain areas of non-pregnant, 15 and 20 day pregnant and 4 day post-partum rats. Striatal 5-HT content was significantly lower in 15 and 20 day pregnant rats than in estrous controls. A significant decr...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965738
更新日期:1990-10-01 00:00:00
abstract::Most inborn errors of tyrosine catabolism produce hypertyrosinemia. Neurological manifestations are variable and some patients are developmentally normal, while others show different degrees of developmental retardation. Considering that current data do not eliminate the possibility that elevated levels of tyrosine an...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-013-1078-0
更新日期:2013-08-01 00:00:00
abstract::The effect of postdecapitative ischemia (5 min at 37 degrees) and hypoxia (5% O2, 95% N2, 30 min) on the distribution of protein radioactivity in the cellular fractions of guinea pig cerebral cortex was studied. Ischemic conditions resulted in the increase of total radioactivity level and protein content in the mitoch...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00963828
更新日期:1979-02-01 00:00:00
abstract::Danhong injection, a Chinese Materia Medica standardized product extracted from Radix Salviae miltiorrhizae and Flos Carthami tinctorii, is used extensively for the treatment of cerebrovascular diseases such as acutely cerebral infarction in clinic. In this study, we further investigated the mechanisms of Danhong inje...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-014-1384-1
更新日期:2014-09-01 00:00:00
abstract::α-Synuclein (α-Syn) plays a crucial role in the pathophysiology of Parkinson's disease (PD), the degeneration of dopaminergic neurons. Previous studies have shown that α-Syn regulates dopamine synthesis by binding to and inhibiting tyrosine hydroxylase (TH). In neurons, protein phosphatases (PPs) play a prominent role...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-014-1477-x
更新日期:2015-03-01 00:00:00
abstract::We review some of the older literature concerning metabolic turnover of cholesterol in the nervous system. The overall picture is that incorporation of radioactive precursors into brain cholesterol is roughly proportional to the rate of myelination and that, once incorporated, radioactive cholesterol is relatively sta...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/BF02527711
更新日期:1996-04-01 00:00:00
abstract::Hippocampal cells dissociated from 5-day-old rat pups were grown in a suitable chemically defined basal medium, supplemented or not with 3,3',5-triiodo-L-thyronine (T3), [Arg8]-vasopressin (AVP), or both, at different concentrations. Four days after plating, neuron-like cells began to degenerate drastically in the bas...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965924
更新日期:1989-10-01 00:00:00
abstract::Protein misfolding and deposition in the brain are implicated in the etiology of numerous neurodegenerative disorders. Here, organic solutes characteristic of microorganisms adapted to hot environments, were tested on experimental cell models of Huntington's and Parkinson's diseases. Diglycerol phosphate, di-myo-inosi...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-011-0440-3
更新日期:2011-06-01 00:00:00
abstract::Sleep disorders negatively affect cognition and health. Recent evidence has indicated that chromatin remodeling via histone acetylation regulates cognitive function. This study aimed to investigate the possible roles of histone acetylation in sleep deprivation (SD)-induced cognitive impairment. Results of the Morris w...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-016-1937-6
更新日期:2016-09-01 00:00:00
abstract::During a variety of insults to the brain adenine nucleotides are released in large quantities from damaged cells, triggering multiple cellular responses to injury. Here, we evaluated changes in extracellular ATP, ADP and AMP hydrolysis at different times (0-24 hours) after unilateral cortical stab injury (CSI) in adul...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-007-9529-0
更新日期:2008-05-01 00:00:00
abstract::The interactions of S-nitrosoglutathione (GSNO) with the ionotropic glutamate receptors were studied on synaptic membranes isolated from the pig cerebral cortex. GSNO displaced the binding of [3H]glutamate, 3-[(R)-2-carboxypiperazin-4-yl] [3H]propyl-1-phosphonate ([3H]CPP), a competitive N-methyl-D-aspartate (NMDA) an...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1007626230278
更新日期:2000-08-01 00:00:00
abstract::The effect of anticonvulsant drugs was examined on brain GABA levels and GAD and GABA-T activities. The level of GABA was increased by the treatment with diphenylhydantoin. The drug had no effect on GABA-T activity, whereas GAD activity was inhibited. Carbamazepine increased the GABA level but did not effect GAD and G...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964170
更新日期:1984-02-01 00:00:00