Abstract:
:The kinetics and specificity of taurine and beta-alanine uptake were studied in primary cultures of rat astrocytes under identical experimental conditions. The uptake consisted of nonsaturable penetration and saturable high-affinity transport that was strictly sodium dependent. The cells accumulated taurine more effectively than beta-alanine, both the affinity and uptake capacity being greater for taurine. Taurine uptake was competitively inhibited by beta-alanine and GABA, the former being more potent. Also, hypotaurine and 2-guanidinoethanesulphonic acid strongly reduced taurine uptake, but L-2,4-diaminobutyric acid had no significant effect. beta-Alanine uptake was also competitively inhibited by GABA, but the most potent inhibitors were hypotaurine and 2-guanidinoethanesulphonic acid. L-2,4-Diaminobutyric acid was moderately active. The uptake systems for taurine and beta-alanine were thus in principle similar, and they exhibited certain characteristics typical for a neurotransmitter amino acid. The inhibition studies further suggest the existence of only one common transport system for taurine, beta-alanine, and GABA in cultured primary astrocytes. The same uptake system may also be used for hypotaurine.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Holopainen I,Kontro Pdoi
10.1007/BF00967969subject
Has Abstractpub_date
1986-02-01 00:00:00pages
207-15issue
2eissn
0364-3190issn
1573-6903journal_volume
11pub_type
杂志文章abstract::Prion diseases are conformational diseases, many factors are involved in altering the conformation of prion, such as RNA, DNA, pH, and copper etc. However the neurotoxic mechanism of prion diseases is not clear yet. The aim of this study is to investigate the effect of the nucleoprotein complex of RNA and recombinant ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-011-0506-2
更新日期:2011-10-01 00:00:00
abstract::Many studies have demonstrated that apoptosis play an important role in cerebral ischemic pathogenesis and may represent a target for treatment. Neuroprotective effect of quercetin has been shown in a variety of brain injury models including ischemia/reperfusion. It is not clear whether BDNF-TrkB-PI3K/Akt signaling pa...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-012-0871-5
更新日期:2012-12-01 00:00:00
abstract::Endogenous and xenobiotic sulphur-containing convulsant and non-convulsant compounds containing structural moieties of, or bearing a structural resemblance to, GABA and homocysteine were tested in binding studies for their potency in displacing the GABA-mimetic [3H]muscimol from specific, high-affinity sites (Kd = 3.6...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00971006
更新日期:1987-07-01 00:00:00
abstract::A number of recent findings support the notion of mechanistic parallels between Alzheimer disease (AD) and oncogenic processes, specifically, that neurons in AD, like cancer cells, display aberrant mitotic cell cycle re-entry. However, the mechanism that drives postmitotic neurons to reenter cell cycle remains elusive...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-006-9146-3
更新日期:2007-04-01 00:00:00
abstract::Ciliary neurotrophic factor (CNTF) is a promyelinating trophic factor that plays an important role in multiple sclerosis (MS). However, mechanisms by which CNTF expression could be increased in the brain are poorly understood. Recently we have discovered anti-inflammatory and immunomodulatory activities of sodium benz...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-015-1723-x
更新日期:2015-11-01 00:00:00
abstract::The K-stimulated release of [3H]ACh from rat midbrain slices prelabeled by incubation with [3H]choline was dependent on extracellular Ca. Phenobarbital inhibited the K-stimulated [3H]ACh release and the IC50 was equal to that found for K-stimulated endogenous ACh release. These results support the suggestion that barb...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964710
更新日期:1980-07-01 00:00:00
abstract::Long-term intake of aspartame at the acceptable daily dose causes oxidative stress in rodent brain mainly due to the dysregulation of glutathione (GSH) homeostasis. N-Acetylcysteine provides the cysteine that is required for the production of GSH, being effective in treating disorders associated with oxidative stress....
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-014-1360-9
更新日期:2014-09-01 00:00:00
abstract::Secretases degrade amyloid precursor protein (APP) releasing fragments (beta-peptides A beta, A beta x) that assemble to form hallmark extracellular deposits in Alzheimer's disease (AD) correlating with disease severity. As such, secretases supply targets for therapeutic intervention and form the focus of this overvie...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1023/a:1023211323853
更新日期:2003-07-01 00:00:00
abstract::The elevated plus maze (EPM) is an animal model of anxiety used to test the effects of anxioselective drugs. The loss of the anxiolytic effect of drugs during the second exposure to the EPM is called the "one trial tolerance" (OTT) phenomenon. The present study was designed to investigate the relationship between the ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-013-1051-y
更新日期:2013-07-01 00:00:00
abstract::The search for neuronotrophic factors addressing CNS neurons requires CNS neuronal cell cultures to quantitate putative effects on neuronal survival. Investigation of neurons dissociated from several embryonic CNS tissues have shown that their short-term survival requires supplementation of the culture medium with eit...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00973276
更新日期:1988-07-01 00:00:00
abstract::It is an honour to have this opportunity write an article in recognition of the immense contributions of Bruce Ransom to the field of glial research. For me (BAM) personally there are many highlights both as a colleague and a friend that come to mind when I reflect on the many years that I have known Bruce. My own ent...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-017-2315-8
更新日期:2017-09-01 00:00:00
abstract::Chicken neurons in culture display measurable activities of mono- and diacylglycerol lipases. Treatment of chicken neuronal cultures with gangliosides (10(-8)M to 10(-5)M) resulted in a time and dose dependent increase in monoacylglycerol lipase activity. The diacylglycerol lipase showed significant increase in specif...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00966702
更新日期:1991-11-01 00:00:00
abstract::Cloned fragments of members of the Drosophila and mouse major heat shock (hsp70) gene family were used to demonstrate that homologous sequences are present in the rabbit genome. After a physiologically relevant increase in body temperature of 3 degrees C, transcription of inducible hsp70 genes is detected in both the ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964846
更新日期:1985-09-01 00:00:00
abstract::The current studies focus on what mechanisms regulate the concentration of PLP mRNA in cells. The PLP mRNA is very stable and these studies suggest that its stability is regulated by a trans-acting factor specific to oligodendrocytes. In order to test whether the 3'untranslated region (3'UTR) of the PLP mRNA might reg...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1021623700009
更新日期:2002-11-01 00:00:00
abstract::The cellular effects of eleven compounds including chalcone glycosides isolated from Brassica rapa L. 'hidabeni' and their synthetic derivatives were studied in rat pheochromocytoma PC12 cells. Of the compounds tested, 4'-O-β-D-glucopyranosyl-3',4-dimethoxychalcone (A2) significantly increased the levels of the phosph...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-013-1152-7
更新日期:2013-11-01 00:00:00
abstract::Evidence suggests that apoptosis contributes significantly to cell death after cerebral ischemia. Our recent studies that utilized human umbilical cord blood-derived mesenchymal stem cells (hUCBSCs) demonstrated the potential of hUCBSCs to inhibit neuronal apoptosis in a rat model of CNS injury. Therefore, we hypothes...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-014-1341-z
更新日期:2014-08-01 00:00:00
abstract::CNS trauma has been associated with an increase in free radical production, but the cellular sources of this increase or the mechanism involved in the production of free radicals are not known. We, therefore, investigated the effects of trauma on free radical production in cultured neurons, astrocytes and BV-2 microgl...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1014875210852
更新日期:2002-02-01 00:00:00
abstract::The real-time iontophoretic method has measured volume fraction and tortuosity of the interstitial component of extracellular space in many regions and under different conditions. To interpret these data computer models of the interstitial space (ISS) of the brain are constructed by representing cells as Basic Cellula...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-019-02793-6
更新日期:2020-01-01 00:00:00
abstract::The activities of L-glutamate decarboxylase (GAD), GABA-transaminase (GABA-T), choline acetyltransferase (CAT), and cysteic and cysteinesulfinic acids decarboxylase (CAD/CSAD) in putamen and frontal cortex in both Huntington's chorea and normal tissues were measured. The greatest difference between Huntington's and no...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964435
更新日期:1979-10-01 00:00:00
abstract::Rabbits were immunized with the myelin-associated glycoprotein (MAG) that had been purified from isolated rat brain myelin by selective extraction with lithium diiodosalicylate (LIS) and phenol followed by preparative SDS gel electrophoresis. Antibodies to MAG were detected qualitatively by immunodiffusion and quantit...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964417
更新日期:1981-10-01 00:00:00
abstract::The particulars regarding the amine precursor therapy for treatment of neuropsychiatric disorders and their neurochemical background are described historically. Furthermore, peculiar or artificial amino acids which are metabolized into the brain amine or their derivatives and promised their clinical application are al...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/BF02529142
更新日期:1996-02-01 00:00:00
abstract::A prominent feature of brain pathology in Alzheimer's disease is a robust activation of the neuronal lysosomal system and major cellular pathways converging on the lysosome, namely, endocytosis and autophagy. Recent studies that identify a disturbance of the endocytic pathway as one of the earliest known manifestation...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1023/a:1007675508413
更新日期:2000-10-01 00:00:00
abstract::Evidence that brain glutamatergic activity is pathologically elevated in bipolar disorder suggests that mood stabilizers are therapeutic in the disease in part by downregulating glutamatergic activity. Such activity can involve the second messenger, arachidonic acid (AA, 20:4n - 6). We tested this hypothesis with rega...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-008-9700-2
更新日期:2008-11-01 00:00:00
abstract::The pathogenesis of sepsis associated encephalopathy (SAE) is not yet clear: the blood-brain barrier (BBB) disruption has been indicated among the possible causative mechanisms. S100B, a calcium binding protein, originates in the central nervous system but it can be also produced by extra-cerebral sources; it is passi...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-008-9907-2
更新日期:2009-07-01 00:00:00
abstract::The effects of mu, delta, and kappa receptor-agonists on forskolin stimulated cyclic adenosine-3',5'-monophosphate (cAMP) formation were examined in astroglial enriched primary cultures from the cerebral cortex of newborn rats. Intracellular cAMP accumulation was quantified by radioimmunoassay. Morphine was used as a ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF01101714
更新日期:1990-11-01 00:00:00
abstract::Transgenic therapy for central neuralgia faces the problems of low expression and weak targeting and affects superficial but not deep neurons. In this study, we generated a lentivirus vector with human preproenkephalin gene (hPPE) expression driven by the transcriptional amplification strategy system (TAS) and establi...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-020-03068-1
更新日期:2020-09-01 00:00:00
abstract::In this paper, we show that caveolin-1 is abundantly present in a cell line of immortalized gonadotropin-releasing hormone-expressing neurons (GN11). In contrast to GN11, caveolin is undetectable in a cognate cell line of immortalized gonadotropin-releasing hormone-secreting neurons (GT1-7). These two cell lines are c...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1020217309987
更新日期:2002-08-01 00:00:00
abstract::Development of the central somatosensory system is profoundly modulated by the sensory periphery. Cauterization of facial whiskers alters the segregation pattern of barrels in rodents only during a few days just after birth (critical period). Although a molecular basis of the segregation of barrel neurons and the crit...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1022075508176
更新日期:1997-07-01 00:00:00
abstract::The mutual interactions and the effects of GABA on the saturable transport components of taurine and hypotaurine were investigated with mouse brain slices. The low-affinity taurine transport was competitively inhibited by both hypotaurine and GABA. Hypotaurine did not alter the kinetic parameters of high-affinity taur...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964995
更新日期:1983-11-01 00:00:00
abstract::The effects of 1 min of acute hypoxic treatment (1% O2 in N2) on incorporation of [1-14C]arachidonic acid into brain lipids of 16-day-old rats were investigated at 3, 6, and 12 min after intracerebral injection of the labeled fatty acid. The hypoxic-hypoxia condition associated with convulsive seizures caused a decrea...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965474
更新日期:1981-07-01 00:00:00