Characterization of squid enolase mRNA: sequence analysis, tissue distribution, and axonal localization.


:Enolase is a glycolytic enzyme whose amino acid sequence is highly conserved across a wide range of animal species. In mammals, enolase is known to be a dimeric protein composed of distinct but closely related subunits: alpha (non-neuronal), beta (muscle-specific), and gamma (neuron-specific). However, little information is available on the primary sequence of enolase in invertebrates. Here we report the isolation of two overlapping cDNA clones and the putative primary structure of the enzyme from the squid (Loligo pealii) nervous system. The composite sequence of those cDNA clones is 1575 bp and contains the entire coding region (1302 bp), as well as 66 and 207 bp of 5' and 3' untranslated sequence, respectively. Cross-species comparison of enolase primary structure reveals that squid enolase shares over 70% sequence identity to vertebrate forms of the enzyme. The greatest degree of sequence similarity was manifest to the alpha isoform of the human homologue. Results of Northern analysis revealed a single 1.6 kb mRNA species, the relative abundance of which differs approximately 10-fold between various tissues. Interestingly, evidence derived from in situ hybridization and polymerase chain reaction experiments indicate that the mRNA encoding enolase is present in the squid giant axon.


Neurochem Res


Neurochemical research


Chun JT,Gioio AE,Crispino M,Giuditta A,Kaplan BB




Has Abstract


1995-08-01 00:00:00












  • Effect of acute and chronic administration of methylphenidate on mitochondrial respiratory chain in the brain of young rats.

    abstract::Methylphenidate is commonly used for the treatment of attention deficit/hyperactivity disorder. There are still few works regarding the effects of methylphenidate on brain energy metabolism. Thus, in the present study we evaluated the effect of chronic administration of methylphenidate on the activities of mitochondri...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Fagundes AO,Aguiar MR,Aguiar CS,Scaini G,Sachet MU,Bernhardt NM,Rezin GT,Valvassori SS,Quevedo J,Streck EL

    更新日期:2010-11-01 00:00:00

  • Inhibition of neuron-specific CREB dephosphorylation is involved in propofol and ketamine-induced neuroprotection against cerebral ischemic injuries of mice.

    abstract::Propofol and ketamine may provide certain degree of neuroprotection, but the underlying mechanism remains unclear to date. The cAMP response element-binding protein (CREB) was proposed that its phosphorylation at Ser133 (P-CREB) constituted a convergence point involved in neuroprotection. The purpose of this study was...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Shu L,Li T,Han S,Ji F,Pan C,Zhang B,Li J

    更新日期:2012-01-01 00:00:00

  • Aspirin Inhibits Degenerative Changes of Aneurysmal Wall in a Rat Model.

    abstract::Aneurysmal subarachnoid hemorrhage still has a high mortality and morbidity despite notable advances in surgical approaches to cerebral aneurysm (CA). We examined the role of aspirin in vascular inflammation and degeneration. CA was induced in male Sprague-Dawley rats by ligating left common carotid artery and bilater...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Li S,Wang D,Tian Y,Wei H,Zhou Z,Liu L,Wang D,Dong JF,Jiang R,Zhang J

    更新日期:2015-07-01 00:00:00

  • Expression of heat shock genes in fetal and maternal rabbit brain.

    abstract::Cloned fragments of members of the Drosophila and mouse major heat shock (hsp70) gene family were used to demonstrate that homologous sequences are present in the rabbit genome. After a physiologically relevant increase in body temperature of 3 degrees C, transcription of inducible hsp70 genes is detected in both the ...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Brown IR,Lowe DG,Moran LA

    更新日期:1985-09-01 00:00:00

  • RUNX2 Promotes Malignant Progression in Glioma.

    abstract::Glioblastoma (GBM) is the most aggressive and lethal form of brain tumor. However, therapeutic strategies against malignant gliomas have not been completely established. Runt-related transcription factor 2 (Runx2) is an essential gene for skeletal development but its regulatory role in the malignant progression of gli...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Yamada D,Fujikawa K,Kawabe K,Furuta T,Nakada M,Takarada T

    更新日期:2018-11-01 00:00:00

  • Astroglial Mechanisms of Ketamine Action Include Reduced Mobility of Kir4.1-Carrying Vesicles.

    abstract::The finding that ketamine, an anaesthetic, can elicit a rapid antidepressant effect at low doses that lasts for weeks in patients with depression is arguably a major achievement in psychiatry in the last decades. However, the mechanisms of action are unclear. The glutamatergic hypothesis of ketamine action posits that...

    journal_title:Neurochemical research

    pub_type: 杂志文章,评审


    authors: Stenovec M,Božić M,Pirnat S,Zorec R

    更新日期:2020-01-01 00:00:00

  • Levels and uptake of taurine in various brain regions after drug-induced generalized convulsions.

    abstract::In a study of the role of taurine in the genesis of epilepsy the effects of metrazol-induced convulsions on the uptake and distribution of taurine in the brain were measured. In vivo we found no significant uptake of taurine in the mouse brain; in rabbit brain in most areas significant taurine uptake was found. The ph...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Battistin L,Varotto M,Tezzon F,Pistollato L

    更新日期:1979-08-01 00:00:00

  • Mitochondrial function in apoptotic neuronal cell death.

    abstract::Apoptosis can be defined as the regulated death of a cell and is conducted by conserved pathways. Apoptosis of neurons after injury or disease differs from programed cell death, in the sense that neurons in an adult brain are not "meant" to die and results in a loss of function. Thus apoptosis is an honorable process ...

    journal_title:Neurochemical research

    pub_type: 杂志文章,评审


    authors: Haeberlein SL

    更新日期:2004-03-01 00:00:00

  • Recent development in mammalian sialidase molecular biology.

    abstract::This review summarizes the recent research development on mammalian sialidase molecular cloning. Sialic acid-containing compounds are involved in several physiological processes, and sialidases, as glycohydrolytic enzymes that remove sialic acid residues, play a pivotal role as well. Sialidases hydrolyze the nonreduci...

    journal_title:Neurochemical research

    pub_type: 杂志文章,评审


    authors: Monti E,Preti A,Venerando B,Borsani G

    更新日期:2002-08-01 00:00:00

  • Mechanism of Neuroprotection Against Experimental Spinal Cord Injury by Riluzole or Methylprednisolone.

    abstract::Any spinal cord injury carries the potential for persistent disability affecting motor, sensory and autonomic functions. To prevent this outcome, it is highly desirable to block a chain of deleterious reactions developing in the spinal areas immediately around the primary lesion. Thus, early timing of pharmacological ...

    journal_title:Neurochemical research

    pub_type: 杂志文章,评审


    authors: Sámano C,Nistri A

    更新日期:2019-01-01 00:00:00

  • Inhibition of polyphosphoinositide phosphodiesterase by aminoglycoside antibiotics.

    abstract::The calcium-activated phosphodiesteratic hydrolysis of 32P-labeled phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 4-phosphate in prelabeled nerve ending membranes is inhibited by the aminoglycosides neomycin and gentamicin, and to a lesser extent, by streptomycin. The inhibition is overcome by increasi...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Van Rooijen LA,Agranoff BW

    更新日期:1985-08-01 00:00:00

  • [3H]ketanserin binding in human brain postmortem.

    abstract::This study aimed at comparing the binding characteristics of [3H]ketanserin, a high-affinity serotonin 2A (5-HT2A) receptor antagonist, in the prefrontal cortex, hippocampus and striatum of human brain post-mortem. The results indicated the presence of a single population of binding sites in all the regions investigat...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Marazziti D,Rossi A,Palego L,Giannaccini G,Naccarato A,Lucacchini A,Cassano GB

    更新日期:1997-06-01 00:00:00

  • Human Remyelination Promoting Antibody Stimulates Astrocytes Proliferation Through Modulation of the Sphingolipid Rheostat in Primary Rat Mixed Glial Cultures.

    abstract::Remyelination promoting human IgMs effectively increase the number of myelinated axons in animal models of multiple sclerosis. Hence, they ultimately stimulate myelin production by oligodendrocytes (OLs); however, their exact mechanism of action remains to be elucidated, and in particular, it remains unclear whether t...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Grassi S,Giussani P,Prioni S,Button D,Cao J,Hakimi I,Sarmiere P,Srinivas M,Cabitta L,Sonnino S,Prinetti A

    更新日期:2019-06-01 00:00:00

  • Hydrogen peroxide induces lysosomal protease alterations in PC12 cells.

    abstract::Alterations in lysosomal proteases have been implicated in many neurodegenerative diseases. The current study demonstrates a concentration-dependent decrease in PC12 cell viability and transient changes in cystatin C (CYSC), cathepsin B (CATB), cathepsin D (CATD) and caspase-3 following exposure to H2O2. Furthermore, ...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Lee DC,Mason CW,Goodman CB,Holder MS,Kirksey OW,Womble TA,Severs WB,Palm DE

    更新日期:2007-09-01 00:00:00

  • Non-specific esterases and esterproteases in masticatory muscles from the muscular dystrophic mouse.

    abstract::With the aid of histochemical and electrophoretic techniques activities for esterase and esterprotease were investigated in the digastric and masseter muscles from normal and dystrophic mice. The substrates used were alpha-naphthyl acetate and N-acetyl-L-alanine alpha-naphthyl ester. According to the microscopic obser...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Kirkeby S,Moe D,Vilmann H

    更新日期:1989-03-01 00:00:00

  • Process formation in astrocytes: modulation of cytoskeletal proteins.

    abstract::Studies on primary astrocytes cultured in vitro have shown that process formation involves changes in cytoskeletal proteins and release of tension on the substratum. Actin filament reorganization has previously been found to be the major cytoskeletal change occurring during process formation. These changes are relativ...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Padmanabhan J,Shelanski ML

    更新日期:1998-03-01 00:00:00

  • Baicalein Ameliorates Epilepsy Symptoms in a Pilocarpine-Induced Rat Model by Regulation of IGF1R.

    abstract::Treatment for epilepsy, particularly temporal lobe epilepsy, is challenging. Baicalein has multiple effects, including anti-inflammatory action. However, little is known about its efficacy in treatment of epilepsy. In this study, we established a pilocarpine-induced rat model and used it for assessment of baicalein ef...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Fu P,Yuan Q,Sun Y,Wu X,Du Z,Li Z,Yu J,Lv K,Hu J

    更新日期:2020-12-01 00:00:00

  • The expression of several mitochondrial and nuclear genes encoding the subunits of electron transport chain enzyme complexes, cytochrome c oxidase, and NADH dehydrogenase, in different brain regions in Alzheimer's disease.

    abstract::In this study, changes of the expression of two mitochondrial and two nuclear genes encoding the subunits of cytochrome c oxidase (CO) and NADH dehydrogenase (ND) were studied in the hippocampus, inferior parietal lobule, and cerebellum of 10 Alzheimer's disease (AD) and 10 age-matched control subjects. The altered pr...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Aksenov MY,Tucker HM,Nair P,Aksenova MV,Butterfield DA,Estus S,Markesbery WR

    更新日期:1999-06-01 00:00:00

  • RASgrf1, a Potential Methylatic Mediator of Anti-epileptogenesis?

    abstract::Epileptogenesis, induced by status epilepticus (SE), is a chronic process, and intervention in this progress may prevent chronic epilepsy. It has been proposed that DNA methylation might be related with epileptogenesis. RASgrf1 has a differentially methylated region at the promoter which can silence gene expression. W...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Bao Y,Chen X,Wang L,Zhou J,Fu X,Wang X,Xiao Z

    更新日期:2018-10-01 00:00:00

  • Mechanisms of leu-enkephalin hydrolysis in human plasma.

    abstract::The present work describes the kinetics of enkephalin hydrolysis by plasma enzymes and the fragmentation pattern of both the parent peptide and of the first hydrolysis by-products. The degradation kinetics were followed by positive identification of the hydrolysis fragments by chromatographic methods, by amino acid an...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Roscetti G,Possenti R,Bassano E,Roda LG

    更新日期:1985-10-01 00:00:00

  • Branched chain keto-acids exert biphasic effects on alpha-ketoglutarate-stimulated respiration in intact rat liver mitochondria.

    abstract::Pathophysiological concentrations of branched chain keto-acids (BCKAs), such as those that occur in maple syrup urine disease, inhibit oxygen consumption in liver homogenates and brain slices and the enzymatic activity of alpha-ketoglutarate- and pyruvate dehydrogenase complexes. Consistent with previous work, studies...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Shestopalov AI,Kristal BS

    更新日期:2007-04-01 00:00:00

  • Clozapine and some other antipsychotic drugs may preferentially block the same subset of GABA(A) receptors.

    abstract::Selective blockade of a subset of GABA(A) receptors may be involved in the antipsychotic effects of Clozapine and several other antipsychotic drugs. Seven antipsychotic drugs, and 11 drugs classified as antidepressants that only partially reverse the inhibitory effect of 1 microM GABA on [35S]TBPS binding, do not yiel...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Squires RF,Saederup E

    更新日期:1997-02-01 00:00:00

  • The effects of transmitters on the affinity of the insoluble fraction of ox brain for Na+ and K+.

    abstract::The insoluble fraction of ox-brain, which had previously been shown to have a non-linear affinity for Na+ and K+, was prepared. Acetylcholine (1 x 10(-8) mol/l and 1 x 10(-7) mol/l) reduced the affinity of the fraction for Na+ and K+ to zero, while at 1 x 10(-6) mol/l, the affinity for the cations was almost as high a...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Ahmed N,Hillman H

    更新日期:1989-02-01 00:00:00

  • Estradiol attenuates tau hyperphosphorylation induced by upregulation of protein kinase-A.

    abstract::Protein kinase A (PKA) plays a crucial role in tau hyperphosphorylation, an early event of Alzheimer disease (AD), and 17beta-estradiol replacement in aging women forestalls the onset of AD. However, the role of estradiol in PKA-induced tau hyperphosphorylation is not known. Here, we investigated the effect of 17beta-...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Liu XA,Zhu LQ,Zhang Q,Shi HR,Wang SH,Wang Q,Wang JZ

    更新日期:2008-09-01 00:00:00

  • Naphthalenesulfonamide derivatives ML9 and W7 inhibit catecholamine secretion in intact and permeabilized chromaffin cells.

    abstract::The role of protein phosphorylation in catecholamine secretion from bovine adrenomedullary chromaffin cells was studied using different protein kinase inhibitors. Naphthalenesulfonamide derivatives as ML9 and ML7, more specific for the myosin light chain kinase, and the calmodulin antagonist W7 inhibited catecholamine...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Reig JA,Viniegra S,Ballesta JJ,Palmero M,Guitierrez LM

    更新日期:1993-03-01 00:00:00

  • Leucine: effector of phosphate activated glutaminase in rat cerebral cortex.

    abstract::Phosphate activated glutaminase (PAG) was assayed in whole homogenate and synaptosomes of cerebral cortex from normal or fasted for 120 h rats. The specific activity (s.a.) of PAG was found diminished by 25% in the whole homogenate from the fasted animals compared to the normal. On the contrary, fasting did not affect...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Lellos V,Tselentis V,Galanopoulos E,Philippidis H,Palaiologos G

    更新日期:1991-01-01 00:00:00

  • Decreased GABA receptor binding in the cerebral cortex of insulin induced hypoglycemic and streptozotocin induced diabetic rats.

    abstract::Hypoglycemia is the major problem to blood glucose homeostasis in treatment of diabetes and is associated with severe irreversible consequences including seizures, coma and death. GABAergic inhibitory function in the cerebral cortex plays an important role in controlling the excitability and responsiveness of cortical...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Antony S,Kumar TP,Kuruvilla KP,George N,Paulose CS

    更新日期:2010-10-01 00:00:00

  • Glutamate-glutamine cycle and aging in striatum of the awake rat: effects of a glutamate transporter blocker.

    abstract::This study investigated the effects of aging on the actions of a specific glutamate reuptake blocker, L-trans-pyrrolidine-2, 4-dicarboxylic acid (PDC), in extracellular glutamate and glutamine in striatum of the awake rat. Microdialysis experiments were performed on young (2-3 months), middle-aged (12-14 months), aged...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Segovia G,Del Arco A,Prieto L,Mora F

    更新日期:2001-01-01 00:00:00

  • β-Hydroxybutyrate Boosts Mitochondrial and Neuronal Metabolism but is not Preferred Over Glucose Under Activated Conditions.

    abstract::The ketone body, β-hydroxybutyrate (βOHB), is metabolised by the brain alongside the mandatory brain fuel glucose. To examine the extent and circumstances by which βOHB can supplement glucose metabolism, we studied guinea pig cortical brain slices using increasing concentrations of [U-13C]D-βOHB in conjunction with [1...

    journal_title:Neurochemical research

    pub_type: 杂志文章


    authors: Achanta LB,Rowlands BD,Thomas DS,Housley GD,Rae CD

    更新日期:2017-06-01 00:00:00

  • Association of SOD2, GPX1, CAT, and TNF genetic polymorphisms with oxidative stress, neurochemistry, psychopathology, and extrapyramidal symptoms in schizophrenia.

    abstract::There is a growing body of evidence confirming the involvement of oxidative stress and inflammation in pathogenesis of schizophrenia. Inter-individual variation in antioxidant capacity caused by different genetic profile could potentially influence patient's susceptibility to oxidative damage. In this study we evaluat...

    journal_title:Neurochemical research

    pub_type: 杂志文章,随机对照试验


    authors: Bošković M,Vovk T,Saje M,Goričar K,Dolžan V,Kores Plesničar B,Grabnar I

    更新日期:2013-02-01 00:00:00