Characterization of squid enolase mRNA: sequence analysis, tissue distribution, and axonal localization.

Abstract:

:Enolase is a glycolytic enzyme whose amino acid sequence is highly conserved across a wide range of animal species. In mammals, enolase is known to be a dimeric protein composed of distinct but closely related subunits: alpha (non-neuronal), beta (muscle-specific), and gamma (neuron-specific). However, little information is available on the primary sequence of enolase in invertebrates. Here we report the isolation of two overlapping cDNA clones and the putative primary structure of the enzyme from the squid (Loligo pealii) nervous system. The composite sequence of those cDNA clones is 1575 bp and contains the entire coding region (1302 bp), as well as 66 and 207 bp of 5' and 3' untranslated sequence, respectively. Cross-species comparison of enolase primary structure reveals that squid enolase shares over 70% sequence identity to vertebrate forms of the enzyme. The greatest degree of sequence similarity was manifest to the alpha isoform of the human homologue. Results of Northern analysis revealed a single 1.6 kb mRNA species, the relative abundance of which differs approximately 10-fold between various tissues. Interestingly, evidence derived from in situ hybridization and polymerase chain reaction experiments indicate that the mRNA encoding enolase is present in the squid giant axon.

journal_name

Neurochem Res

journal_title

Neurochemical research

authors

Chun JT,Gioio AE,Crispino M,Giuditta A,Kaplan BB

doi

10.1007/BF00970738

subject

Has Abstract

pub_date

1995-08-01 00:00:00

pages

923-30

issue

8

eissn

0364-3190

issn

1573-6903

journal_volume

20

pub_type

杂志文章
  • Cell signaling abnormalities may drive neurodegeneration in familial Alzheimer disease.

    abstract::Presenilins (PSs) are catalytic components of the γ-secretase complex that produces Aβ peptides. Substrates of γ-secretase are membrane-bound protein fragments deriving from the cleavage of extracellular sequence of cell surface proteins. APP-derived γ-secretase substrates are cleaved at gamma (γ) sites to produce Aβ ...

    journal_title:Neurochemical research

    pub_type: 杂志文章,评审

    doi:10.1007/s11064-013-1003-6

    authors: Robakis NK

    更新日期:2014-01-01 00:00:00

  • Comparison between drug-induced and K⁺-induced changes in molar acid extrusion fluxes (JH⁺) and in energy consumption rates in astrocytes.

    abstract::Neuronal excitation leads to an increase of the extracellular K(+) concentration ([K(+)]o) in brain. This increase has at least two energy-consuming consequences: (1) a depolarization-mediated change in intracellular pH (pHi) in astrocytes due to depolarization-mediated increased activity of the acid-extruding Na(+)/b...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/s11064-013-1149-2

    authors: Song D,Man Y,Li B,Xu J,Hertz L,Peng L

    更新日期:2013-11-01 00:00:00

  • Molecular signals for development of neuronal circuitry in the retina.

    abstract::In this review article, we summarize recently accumulated knowledge regarding the molecular mechanisms, which control retinal development. Retinal neurons are born in two waves of cytogenesis. In the first wave, neurons of cone circuitry are generated, whereas in the second wave, rod circuitry is added. Neurons genera...

    journal_title:Neurochemical research

    pub_type: 杂志文章,评审

    doi:10.1023/a:1007696112956

    authors: Sharma RK,Johnson DA

    更新日期:2000-10-01 00:00:00

  • Glial metabolism of valine.

    abstract::The three essential amino acids, valine, leucine and isoleucine, constitute the group of branched-chain amino acids (BCAAs). BCAAs are rapidly taken up into the brain parenchyma, where they serve several distinct functions including that as fuel material in brain energy metabolism. As one function of astrocytes is con...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/s11064-008-9895-2

    authors: Murín R,Mohammadi G,Leibfritz D,Hamprecht B

    更新日期:2009-07-01 00:00:00

  • Effects of beta-alanine treatment on the taurine and DNA content of the rat heart and retina.

    abstract::Experimental evidence has suggested that the high endogenous levels of taurine found in the rat heart and retina are maintained to a large extent by transport processes out of the blood, rather than by endogenous biosynthesis. When these high levels are depleted, dysfunction ensues. In vitro studies have shown that be...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/BF00970775

    authors: Lake N,De Marte L

    更新日期:1988-10-01 00:00:00

  • Differential expression of syntaxin 1A and 1B by noradrenergic and adrenergic chromaffin cells.

    abstract::The expression and localization of syntaxin isoforms 1A and 1B in adrenergic and noradrenergic chromaffin cells were examined by both immunoblot analysis and confocal immunofluorescence microscopy. Syntaxin 1A was found in higher levels in noradrenergic cells, whereas syntaxin 1B was similarly expressed in most noradr...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1023/a:1025658005937

    authors: Baltazar G,Carvalho AP,Duarte EP

    更新日期:2003-10-01 00:00:00

  • Cytokines, signal transduction, and inflammatory demyelination: review and hypothesis.

    abstract::The mechanism of focal demyelination in multiple sclerosis has been a long-standing enigma of this disorder. Cytokines, a diverse family of signalling molecules, are viewed as potential mediators of the process based on clinical observations and studies with animal models and tissue/cell culture systems. Myelin and ol...

    journal_title:Neurochemical research

    pub_type: 杂志文章,评审

    doi:10.1023/a:1022493013904

    authors: Ledeen RW,Chakraborty G

    更新日期:1998-03-01 00:00:00

  • Electrophysiological characterisation of human umbilical cord blood-derived mesenchymal stem cells induced by olfactory ensheathing cell-conditioned medium.

    abstract::Umbilical cord blood-derived marrow stromal cells (UCB-MSCs) with high proliferation capacity and immunomodulatory properties are considered to be a good candidate for cell-based therapies. But until now, little work has been focused on the differentiation of UCB-MSCs. In this work, UCB-MSCs were demonstrated to be ne...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/s11064-013-1186-x

    authors: Zeng Y,Rong M,Liu Y,Liu J,Lu M,Tao X,Li Z,Chen X,Yang K,Li C,Liu Z

    更新日期:2013-12-01 00:00:00

  • SOD3 Ameliorates H2O2-Induced Oxidative Damage in SH-SY5Y Cells by Inhibiting the Mitochondrial Pathway.

    abstract::This study was designed to investigate the protective effects of extracellular superoxide dismutase (SOD3) against hydrogen peroxide (H2O2) induced damage in human neuroblastoma SH-SY5Y cells and to elucidate the mechanisms responsible for this beneficial effect. SOD3-overexpressing SH-SY5Y cells were generated by ade...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/s11064-016-1897-x

    authors: Yang R,Wei L,Fu QQ,Wang H,You H,Yu HR

    更新日期:2016-07-01 00:00:00

  • Neurotransmitter enzyme activities in neurons purified by bulk-isolation.

    abstract::Neurons, purified by bulk-isolation procedures from 10 day-old rat brain, are 80-90% homogeneous. Contaminants are primarily blood vessels and occasional oligodendroglia. All sizes and shapes of neurons are obtained, as they are isolated from all areas of the brain, except the cerebellum. Neurotransmitter enzyme activ...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/BF00971368

    authors: Chechik T,Poduslo SE

    更新日期:1987-01-01 00:00:00

  • NPD1 induction of retinal pigment epithelial cell survival involves PI3K/Akt phosphorylation signaling.

    abstract::Neuroprotectin D1 (NPD1), a docosahexaenoic acid (DHA)-derived lipid mediator, promotes survival in cells exposed to oxidative stress by inducing the activity of anti-inflammatory mediators and suppressing the expression of pro-inflammatory genes. Though retinal pigment epithelial (RPE) cells naturally produce NPD1 fr...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/s11064-010-0351-8

    authors: Halapin NA,Bazan NG

    更新日期:2010-12-01 00:00:00

  • Effects of L-carnitine on the formation of fatty acid ethyl esters in brain and peripheral organs after short-term ethanol administration in rat.

    abstract::A study was undertaken in rats to evaluate the effects of short-term oral ethanol administration on the levels of fatty acid ethyl esters (FAEE) in brain and peripheral organs in the presence and absence of pretreatment with L-carnitine. Administration of ethanol to rats for seven days resulted in fatty acid ethyl est...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1023/a:1020984114824

    authors: Calabrese V,Rizza V

    更新日期:1999-01-01 00:00:00

  • Role of selenium on calcium signaling and oxidative stress-induced molecular pathways in epilepsy.

    abstract::Epilepsy is one of the oldest neurological conditions known to humankind. It is known that oxidative stress and generation of reactive oxygen species are a cause and consequence of epileptic seizures. Although recent years have seen tremendous progress in the molecular biology and metabolism of selenium, we still know...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/s11064-009-0015-8

    authors: Nazıroglu M

    更新日期:2009-12-01 00:00:00

  • Effects of NMDA on carbachol-stimulated phosphatidylinositol resynthesis in rat brain cortical slices.

    abstract::N-methyl-D-aspartate (NMDA) inhibits carbachol-stimulated phosphoinositide breakdown in rat brain cortical slices but not in isolated membranes (1). To gain insight into the mechanisms, we examined the effects of NMDA on carbachol-stimulated [3H]inositol phosphate and intermediates of phosphatidylinositol cycle accumu...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1023/a:1022426204583

    authors: Lee HM,Huang CL

    更新日期:1997-05-01 00:00:00

  • Activation of liver X receptor decreases BACE1 expression and activity by reducing membrane cholesterol levels.

    abstract::The synthetic Liver X receptor (LXR) activator T0901317 has been reported to exert neuroprotective effect in Alzheimer's disease, but the relationship between LXR activation and beta-site amyloid precursor protein cleaving enzyme 1 (BACE-1) remains uncertain. This study investigated the effect of T0901317 on membrane ...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/s11064-011-0513-3

    authors: Cui W,Sun Y,Wang Z,Xu C,Xu L,Wang F,Chen Z,Peng Y,Li R

    更新日期:2011-10-01 00:00:00

  • The unfolded protein response is triggered in rat neurons of the dorsal raphe nucleus after single-prolonged stress.

    abstract::The dorsal raphe nucleus (DRN) has been suggested playing an important role in the pathophysiology of post-traumatic stress disorder (PTSD), however the underlying cellular mechanisms are not fully understood. The endoplasmic reticulum (ER) is a critical organelle for synthesis of membrane and secretory proteins, and ...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/s11064-014-1262-x

    authors: Xie J,Han F,Shi Y

    更新日期:2014-04-01 00:00:00

  • GABA metabolism in Venezuelan equine encephalomyelitis virus infection.

    abstract::Mice infected with the Venezuelan equine encephalomyelitis virus showed a significant decrease in the GABA content of cerebral hemispheres. Activity of the enzyme which synthetizes GABA, glutamate decarboxylase, is also reduced in whole cerebral hemispheres, neostriatum, and frontal cortex of infected animals, as comp...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/BF00964333

    authors: Bonilla E,Ryder E,Ryder S

    更新日期:1980-02-01 00:00:00

  • Inhibition of nitric oxide synthase with 7-nitroindazole does not modify early metabolic recovery following focal cerebral ischemia in rats.

    abstract::Nitric oxide has been strongly implicated in the development of tissue infarction in response to focal cerebral ischemia. Nitric oxide and its derivatives can inhibit components of the electron transport chain, providing a likely target for these substances in ischemic and post-ischemic brain. Lactate content is incre...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/s11064-006-9160-5

    authors: Helps SC,Sims NR

    更新日期:2007-04-01 00:00:00

  • Characterization of divalent cation-induced [3H]acetylcholine release from EGTA-treated rat hippocampal synaptosomes.

    abstract::Calcium-naive synaptosomes were used to assess the effects of divalent cations on [3H]acetylcholine release from rat hippocampal homogenates. Following equilibration with calcium-free buffer (containing 10 microM EGTA), calcium reversibly increased [3H]acetylcholine efflux (up to five-fold) while causing no measurable...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/BF00966598

    authors: Vickroy TW,Schneider CJ

    更新日期:1991-10-01 00:00:00

  • Incorporation of [3H]ethanolamine into acetylcholine by a human cholinergic neuroblastoma clone.

    abstract::Human neuroblastoma cholinergic LA-N-2 cells were used as an experimental model to test the possibility that the methylation of phosphoethanolamine (PEtn) to phosphocholine (PCho) and free choline (Cho) (Andriamampandry et al. 1989) could contribute to acetylcholine (AcCho) synthesis. LA-N-2 cells were incubated with ...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/BF00966721

    authors: Haidar NE,Carrara M,Andriamampandry C,Kanfer JN,Freysz L,Dreyfus H,Massarelli R

    更新日期:1994-01-01 00:00:00

  • Short-Term Differentiation of Glioblastoma Stem Cells Induces Hypoxia Tolerance.

    abstract::Glioblastoma is the most common and malignant brain cancer. In spite of surgical removal, radiation and chemotherapy, this cancer recurs within short time and median survival after diagnosis is less than a year. Glioblastoma stem cells (GSCs) left in the brain after surgery is thought to explain the inevitable recurre...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/s11064-016-1868-2

    authors: Skjellegrind HK,Fayzullin A,Johnsen EO,Eide L,Langmoen IA,Moe MC,Vik-Mo EO

    更新日期:2016-07-01 00:00:00

  • Inhibition of GABAA ligand-gated Cl- channels by zinc in adult rat brain: a regional study.

    abstract::Zinc (Zn2+) was shown to invariably inhibit muscimol-stimulated 36Cl- uptake by synaptoneurosomes in the cerebral cortex, hippocampus and cerebellum. The Zn2+ sensitivity of the GABAA receptor-gated 36Cl- uptake in the cerebral cortex was comparable to that in the hippocampus, whereas the uptake in the cerebellum was ...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/BF02532346

    authors: Ito Y,Segawa K,Kobayashi M,Fukuda H

    更新日期:1996-08-01 00:00:00

  • Staurosporine-induced growth inhibition of glioma cells is accompanied by altered expression of cyclins, CDKs and CDK inhibitors.

    abstract::Staurosporine was found to bring about complete growth inhibition of human glioma cell lines. U87 MG cells were arrested in S phase while U373 MG cells in G2/M phase on staurosporine treatment. Consistent with this observation, no change in G1 phase regulators viz., Cyclin D1, D3 and CDK4 was seen on staurosporine tre...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/s11064-006-9068-0

    authors: Harmalkar MN,Shirsat NV

    更新日期:2006-05-01 00:00:00

  • Studies on new, centrally active and reversible acetylcholinesterase inhibitors.

    abstract::We have synthesized the tertiary amines of pyridostigmine and neostigmine, 3-pyridinol dimethylcarbamate (norpyridostigmine) and 3-dimethylaminophenol dimethylcarbamate (norneostigmine) respectively, and we have tested their abilities to cross the blood-brain barrier and inhibit mouse brain AChE activity. The in vivo ...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/BF00973747

    authors: Arnal F,Coté LJ,Ginsburg S,Lawrence GD,Naini A,Sano M

    更新日期:1990-06-01 00:00:00

  • Format determinants of synthetic myelin basic protein peptide S82 mimicked by a mixture of synthetic peptides S8 and S79.

    abstract::Antibodies to synthetic myelin basic protein peptide S82 (TTHYG-SLPQKAQGHRPQDEG) did not react with synthetic peptide S8 (GSLPQKAQGHRPQDENG) and only partially so with synthetic peptide S79 (AQGHRPQDEG); however, the antibodies did react to a considerable extent with an equimolar mixture of S8 and S79. Since the anti-...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/BF00964671

    authors: Day ED,Hashim GA

    更新日期:1984-10-01 00:00:00

  • Effect of hyperosmotic conditions on the expression of the betaine-GABA-transporter (BGT-1) in cultured mouse astrocytes.

    abstract::The adaptation of cells to hyperosmotic conditions involves accumulation of organic osmolytes to achieve osmotic equilibrium and maintenance of cell volume. The Na+ and Cl(-)-coupled betaine/GABA transporter, designated BGT-1, is responsible for the cellular accumulation of betaine and has been proposed to play a role...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/s11064-005-6879-3

    authors: Olsen M,Sarup A,Larsson OM,Schousboe A

    更新日期:2005-06-01 00:00:00

  • A hemagglutinin specific for sialic acids in a rat brain synaptic vesicle-enriched fraction.

    abstract::A hemagglutinating activity was detected in a synaptic vesicle-enriched fraction prepared from adult rat brain, using trypsinized glutaraldehyde-fixed rabbit erythrocytes. The specific activity of the fraction, in two series of experiments, was 7.5 and 16-fold higher than in the other subcellular fractions. The activi...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/BF00971856

    authors: Popoli M,Mengano A

    更新日期:1988-01-01 00:00:00

  • Retraction Note to: MicroRNA-322 Cluster Promotes Tau Phosphorylation via Targeting Brain-Derived Neurotrophic Factor.

    abstract::The Editors have retracted this article [1] following an investigation conducted by the journal. After publication concerns were raised regarding interpretation of the data presented in Fig. 4. The Editors requested additional data and clarification to confirm interpretation of data results. After further review, the ...

    journal_title:Neurochemical research

    pub_type: 杂志文章,撤回出版物

    doi:10.1007/s11064-020-03077-0

    authors: Zhang J,Liu Z,Pei Y,Yang W,Xie C,Long S

    更新日期:2020-09-01 00:00:00

  • Isolation of human nerve growth factor from placental tissue.

    abstract::Nerve growth factor (NGF) has been isolated from human placental tissue. Using the chicken embryo dorsal root ganglia assay, we determined levels of NGF activity for the amnion, placental cotyledons, cord serum, fetal serum, and maternal serum. The highest levels of NGF activity were measured in placental cotyledons. ...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/BF00964058

    authors: Goldstein LD,Reynolds CP,Perez-Polo JR

    更新日期:1978-04-01 00:00:00

  • Treadmill Exercise Promotes Neurogenesis in Ischemic Rat Brains via Caveolin-1/VEGF Signaling Pathways.

    abstract::Using a model of middle cerebral artery occlusion (MCAO), we have previously demonstrated that treadmill exercise promotes angiogenesis in the ischemic penumbra through caveolin-1/VEGF signaling pathways. However, the function of caveolin-1/VEGF signaling in neurogenesis after MCAO has not been determined. In this stu...

    journal_title:Neurochemical research

    pub_type: 杂志文章

    doi:10.1007/s11064-016-2081-z

    authors: Zhao Y,Pang Q,Liu M,Pan J,Xiang B,Huang T,Tu F,Liu C,Chen X

    更新日期:2017-02-01 00:00:00