Abstract:
:Radioactive azide derivatives of tetrodotoxin (TTX) were synthesized using 2-nitro-4-azidephenyl-[3H]beta alanine for the purpose of photolabeling of the Na channel. Three azide derivatives, N1, N2 and N3, were separated by ion exchange chromatography on Bio-Rex 70 resin and reversed phase high performance liquid chromatography. N3 was more stable and obtained at a higher yield than the other two derivatives. Bioactivity of N3 was one-twentieth of that of TTX. N3 showed reversible binding to membranes of Electrophorus electricus electroplax in the dark with Kd = 30 nM and B max = 5.2 pmol/mg protein. By photoirradiation, irreversible binding of N3 to the membranes was observed. A N3 binding component was solubilized by lubrol PX and partially purified from the electroplax membranes by Sephadex G25 and Sepharose 6B column chromatography. The component, purified 500 fold from the starting membranes, showed molecular weight of 10,000.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Uehara S,Uyemura Kdoi
10.1007/BF00965886subject
Has Abstractpub_date
1985-08-01 00:00:00pages
1119-28issue
8eissn
0364-3190issn
1573-6903journal_volume
10pub_type
杂志文章abstract::In vitro synthesis of myelin proteolipid protein (PLP) was explored at different ages using rat brain total homogenates, incubated for 30 min with [3H]glycine. Total proteolipids, extracted from the incubated samples, were separated by SDS-PAGE and the radioactivity was measured in the band corresponding to myelin PLP...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00973044
更新日期:1984-09-01 00:00:00
abstract::We investigated the ability of hydrogen peroxide (H2O2) to cause apoptotic cell death in cultured rat forebrain neurons and the potential mechanisms by which oxidative stress triggers delayed neuronal death. H2O2 (25 microM for 5 min) reduced cell viability to 34.5 +/- 8.3% of untreated controls 20 h after exposure, a...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1022403224901
更新日期:1997-03-01 00:00:00
abstract::Transgenic therapy for central neuralgia faces the problems of low expression and weak targeting and affects superficial but not deep neurons. In this study, we generated a lentivirus vector with human preproenkephalin gene (hPPE) expression driven by the transcriptional amplification strategy system (TAS) and establi...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-020-03068-1
更新日期:2020-09-01 00:00:00
abstract::The stability of somatostatin (SS) in brain tissue was studied in human material obtained post-mortem and in the rat. In both human and rat brain, loss of SS was found to occur in tissue frozen to -70 degrees C. In the rat, this loss varied from 26 to 70 percent depending on the type of tissue processing used. These d...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00973289
更新日期:1988-06-01 00:00:00
abstract::Using a model of middle cerebral artery occlusion (MCAO), we have previously demonstrated that treadmill exercise promotes angiogenesis in the ischemic penumbra through caveolin-1/VEGF signaling pathways. However, the function of caveolin-1/VEGF signaling in neurogenesis after MCAO has not been determined. In this stu...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-016-2081-z
更新日期:2017-02-01 00:00:00
abstract::Alzheimer's disease (AD) is the most common form of dementia that progressively disrupts neurocognitive function, which has neither cure nor effective treatment. Hypercholesterolemia might be involved in brain alterations that could evolve into AD. The present study aims to evaluate the potential of omega-3, Co-enzyme...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-020-02996-2
更新日期:2020-05-01 00:00:00
abstract::In order to obtain further evidence of putative neurotransmitters in primary sensory neurons and interneurons in the dorsal spinal cord, we have studied the effects of unilateral section of dorsal roots and unilateral occlusion of the dorsal spinal artery on cholinergic enzyme activity and on selected amino acid level...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964851
更新日期:1989-05-01 00:00:00
abstract::The peroxisome proliferator-activated receptor gamma (PPARγ) is a ligand-activated transcriptional factor that belongs to the nuclear hormone receptor superfamily. PPARγ was initially identified through its role in the regulation of glucose and lipid metabolism and cell differentiation. It also influences the expressi...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-014-1377-0
更新日期:2015-02-01 00:00:00
abstract::Activities toward arachidonyl-labelled phospholipase A2 substrates were assayed in fractions of white matter and cerebral cortex from control subjects and in fractions of demyelinated plaque, normal-appearing white matter and cerebral cortex from subjects who died with multiple sclerosis. Membranous activity at pH 8.6...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00992509
更新日期:1995-11-01 00:00:00
abstract::The effects of mu, delta, and kappa receptor-agonists on forskolin stimulated cyclic adenosine-3',5'-monophosphate (cAMP) formation were examined in astroglial enriched primary cultures from the cerebral cortex of newborn rats. Intracellular cAMP accumulation was quantified by radioimmunoassay. Morphine was used as a ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF01101714
更新日期:1990-11-01 00:00:00
abstract::Amyotrophic lateral sclerosis (ALS) is a degenerative disorder caused by motor neuron loss. T-cell intracellular antigen-1 (TIA-1), a cytotoxic T lymphocyte granule-associated RNA binding protein, is a key component of stress granules. However, it remains uncertain whether ALS-causing superoxide dismutase-1 (SOD1) tox...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-020-03137-5
更新日期:2020-12-01 00:00:00
abstract::Evidence that brain glutamatergic activity is pathologically elevated in bipolar disorder suggests that mood stabilizers are therapeutic in the disease in part by downregulating glutamatergic activity. Such activity can involve the second messenger, arachidonic acid (AA, 20:4n - 6). We tested this hypothesis with rega...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-008-9700-2
更新日期:2008-11-01 00:00:00
abstract::Taurine is a semiessential amino acid that is not incorporated into proteins. In mammalian tissues, taurine is ubiquitous and is the most abundant free amino acid in the heart, retina, skeletal muscle, and leukocytes. Taurine reaches up to 50 mM concentration in leukocytes. Taurine has been shown to be tissue-protecti...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1023/b:nere.0000010440.37629.17
更新日期:2004-01-01 00:00:00
abstract::Despite the consensus that activation of TWIK-related spinal cord K+ (TRESK) might contribute to the pathogenesis of chronic pain, the specific mechanisms underlying the transfer and development of pain signals still remain obscure. In the present study, we validated that TRESK was expressed in neurons instead of glia...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-016-2170-z
更新日期:2017-05-01 00:00:00
abstract::Hypothalamic and plasma dopamine-beta-hydroxylase [DBH, EC 1.14.2.1] activites were measured by a coupled radioenzymatic method. Animals representing five experimental groups [intact controls, adrenalectomized, pinealectomized, adrenalectomized pinealectomized, doubly sham-operated] were killed and sampled at 8 times ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965575
更新日期:1978-06-01 00:00:00
abstract::Endogenous phosphorylation of proteins from rat brain synaptosomal plasma membranes was studied in vitro. Cyclic AMP (cAMP) markedly stimulated(32)P incorporation in three protein bands with molecular weights of 75,000, 57,000, and 54,000, respectively. The effect of the behaviorally active peptide ACTH1-24 on this en...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965607
更新日期:1976-12-01 00:00:00
abstract::Systemic inflammation is present in obesity and emerging evidence, primarily from studies using male rodents fed high-fat diets, suggests neuroimmune signaling also is involved. We investigated early changes in neuroimmune signaling during the weight gain that follows ovariectomy in rats. Ovariectomized (OVX) rats wer...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-018-2596-6
更新日期:2018-09-01 00:00:00
abstract::Age-related increase of reactive oxygen species (ROS) is particularly detrimental in postmitotic tissues. Calorie restriction (CR) has been shown to exert beneficial effects, consistent with reduced ROS generation by mitochondria. Many antioxidant compounds also mimic such effects. N-acetyl cysteine (NAC) provides thi...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-005-6867-7
更新日期:2005-06-01 00:00:00
abstract::Association of the candidate gene DLST with late-onset Alzheimer's disease (LOAD) risk has been suggested on the basis of case-control studies. This gene, located on chromosome 14q24.3, encodes a subunit of a mitochondrial component known to be defective in AD, the alpha-ketoglutarate dehydrogenase complex. Positive r...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/b:nere.0000014833.54481.1d
更新日期:2004-03-01 00:00:00
abstract::In a study of the role of taurine in the genesis of epilepsy the effects of metrazol-induced convulsions on the uptake and distribution of taurine in the brain were measured. In vivo we found no significant uptake of taurine in the mouse brain; in rabbit brain in most areas significant taurine uptake was found. The ph...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964640
更新日期:1979-08-01 00:00:00
abstract::The ability of Sendai virosomes or Lipofectin to introduce an AAV vector into primary rat brain astroglial cultures was characterized. The pJDT95npy vector was constructed by inserting rat NPY cDNA downstream from the indigenous AAV p5, p19 and p40 promoters in pJDT95. Lipofectin-mediated transfection with pJDT95npy (...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00967701
更新日期:1994-06-01 00:00:00
abstract::Several lines of evidence suggest that the mechanism underlying drug-induced neuronal apoptosis is initiated by the increased production of reactive oxygen species (ROS). 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), a neurotoxin, has been shown to initiate an apoptotic cascade by increasing ROS in the dopamine...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-013-1208-8
更新日期:2014-02-01 00:00:00
abstract::The current studies focus on what mechanisms regulate the concentration of PLP mRNA in cells. The PLP mRNA is very stable and these studies suggest that its stability is regulated by a trans-acting factor specific to oligodendrocytes. In order to test whether the 3'untranslated region (3'UTR) of the PLP mRNA might reg...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1021623700009
更新日期:2002-11-01 00:00:00
abstract::We found that chronic lithium diet affects the sensitivity of neuroleptic receptors and the content of amino acids in the brain, and that the changes in adult animals differ from those in young rats. Pregnant rats were kept on lithium diet (pellets with 0.21% Li2CO3 and 0.21% NaCl) during the gestation period and the ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964703
更新日期:1983-05-01 00:00:00
abstract::The nerve agent VX has a variable and delayed absorption through the skin, which may have implications for treatment regimens. In the present study, central and peripheral effects of percutaneous VX intoxication were investigated in hairless guinea pigs. Although onset times of clinical signs varied considerably, the ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-007-9508-5
更新日期:2008-02-01 00:00:00
abstract::Mitochondrially bound brain hexokinase is solubilized by anesthetics and this effect has been suggested to contribute to anesthesia. In the present investigation the influence of the metabolic inhibitor 2-deoxy-D-glucose (2-DOG) was studied. An isolated rat brain preparation was used to avoid the contribution of perip...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965526
更新日期:1982-06-01 00:00:00
abstract::Isoleucine, together with leucine and valine, constitutes the group of branched-chain amino acids (BCAAs). BCAAs are transported from the blood into the brain parenchyma, where they can serve several distinct functions. Since brain tissue is known to oxidatively metabolize BCAAs to CO(2), they are considered as fuel m...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-008-9840-4
更新日期:2009-01-01 00:00:00
abstract::The presynaptic dopamine (DA) D2 receptor-mediated regulation of ATP-sensitive potassium (K+ATP) channels was examined in slices of the rat caudate-putamen. When slices were incubated with the specific D2 receptor antagonist (-)-sulpiride (SLP), a concentration-dependent increase of extracellular DA release was observ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1026620316090
更新日期:2000-12-01 00:00:00
abstract::Metabolism of triacylglycerol (TAG) in developing brain has been examined. TAG is a relatively minor fraction of brain lipid in both suckling and adult rats and cannot be accounted for as entrapped blood. When glycerol tri[1-14C]oleate and [2-3H]glycerol trioleate were simultaneously injected intracerebrally into suck...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00966679
更新日期:1981-11-01 00:00:00
abstract::The synaptic glutamate level homeostasis is mainly maintained by the astrocytes membrane bound glutamate transporter type-1 (GLT-1/EAAT2). Alterations in its expression during development and aging and the underlying mechanisms are not well studied. Here, we report that NF-κB interaction was highest in both cerebral a...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-013-1200-3
更新日期:2014-01-01 00:00:00