Abstract：
:The synaptic glutamate level homeostasis is mainly maintained by the astrocytes membrane bound glutamate transporter type-1 (GLT-1/EAAT2). Alterations in its expression during development and aging and the underlying mechanisms are not well studied. Here, we report that NF-κB interaction was highest in both cerebral and cerebellar cortices at day 15 when compared with that at day 0 during development, and it further declined significantly in day 45, and remained unchanged in 20 and 70 weeks mice. On the other hand, N-myc interaction was highest at 0 day which significantly declined at 15-day and interestingly remained unaltered at later ages in both the cortices. This age dependent reciprocal pattern of NF-κB and N-myc interactions with their cognate GLT-1 promoter sequences was further correlated with GLT-1 protein and transcript levels. We found that higher NF-κB interaction with its cognate GLT-1 promoter sequences correlates with up-regulation whereas the higher N-myc interaction correlates with down-regulation of GLT-1 expression during postnatal developmental age up to 15 day, however, such phenomenon was not found in the higher ages from day 45 to 70 weeks. Thus our data suggests a postnatal development- and age dependent differential interaction of transcription factors NF-κB and N-myc to their respective sequences and they act as positive and negative regulator, respectively of GLT-1 gene expression in the brain during early developmental period in both cerebral and cerebellar cortices which might be different in aging of mice.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Gupta RK,Prasad Sdoi
10.1007/s11064-013-1200-3subject
Has Abstractpub_date
2014-01-01 00:00:00pages
150-60issue
1eissn
0364-3190issn
1573-6903journal_volume
39pub_type
杂志文章abstract：:Acidosis (pH 6.0) led to significant decrease in high-affinity choline uptake by rat brain synaptosomes. The effects persisted following pH readjustment (7.4) of the incubation medium, consisting of decrease in both Km and Vmax of the affinity system. pH readjustment coincided with synaptosomal leakage of lactate dehy...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00969699
更新日期：1995-07-01 00:00:00
abstract：:The mutual interactions and the effects of GABA on the saturable transport components of taurine and hypotaurine were investigated with mouse brain slices. The low-affinity taurine transport was competitively inhibited by both hypotaurine and GABA. Hypotaurine did not alter the kinetic parameters of high-affinity taur...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964995
更新日期：1983-11-01 00:00:00
abstract：:We propose the following scheme for cerebral uptake and overall metabolism of glucose in vivo: that brain selects from two pools of glucose anomers in arterial blood, that it takes up excess glucose, that glucose enters the brain tissue as glucose-6-phosphate through the actions of mutarotase and hexokinase, that some...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964568
更新日期：1985-02-01 00:00:00
abstract：:Glioblastoma (GBM) is the most aggressive and lethal form of brain tumor. However, therapeutic strategies against malignant gliomas have not been completely established. Runt-related transcription factor 2 (Runx2) is an essential gene for skeletal development but its regulatory role in the malignant progression of gli...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-018-2626-4
更新日期：2018-11-01 00:00:00
abstract：:Diurnal changes in Chordc1 mRNA were recently described in mouse hypothalamus. This report shows that Chordc1 mRNA changes rhythmically throughout the entire adult brain with highest expression levels occurring around the dark-light transition. The rhythmic cycling pattern of Chordc1 was retained under various light-d...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-006-9271-z
更新日期：2007-02-01 00:00:00
abstract：:The patterns of cholesterol content in chick brain and liver were studied during embryonic development and compared with the variations in the specific activities of mevalonate-activating enzymes during the same period. Total cholesterol content in both embryonic chick brain and liver increased during incubation. The ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964728
更新日期：1983-06-01 00:00:00
abstract：:Regional and developmental changes in metabolite concentrations were measured by 1H NMR spectroscopy and HPLC of perchloric acid extracts from rat brain and eye. The highest concentrations of N-acetylaspartate were found in grey matter as opposed to white matter with concentration increasing with age from neonate to a...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF02532417
更新日期：1996-09-01 00:00:00
abstract：:Parkinson's disease (PD) is a severe neurodegenerative disease characterized by selective loss of dopaminergic neurons, which reduces quality of life of patients and poses a heavy burden to the society. The pathological mechanism of PD remains unclear, and increasing efforts are aimed to solve this problem. MiRNAs are...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-020-03166-0
更新日期：2020-11-12 00:00:00
abstract：:Recent evidence reveals that prolactin gene expression (PRL-GE) in mammotropes occurs in pulses, but the molecular process(es) underlying this phenomenon remains unclear. Earlier, we have identified an E-box (E-box133) in the rat PRL promoter that binds several circadian elements and is critical for this dynamic proce...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-016-1843-y
更新日期：2016-06-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-007-9434-6
更新日期：2008-01-01 00:00:00
abstract：:Cysteinyl leukotrienes (CysLTs) are potent pro-inflammatory and immune modulating lipid mediators involved in inflammatory diseases and were boosted in human brain after acute phase of cerebral ischemia. The antagonism of CysLTs receptors may offer protection against ischemic damage. Therefore it seemed interesting to...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-014-1478-9
更新日期：2015-01-01 00:00:00
abstract：:The inflammatory response mediated by microglia in the central nervous system is closely related to epilepsy. Notch signaling plays an important role in the microglial activation during hypoxia. This study aimed to investigate whether Notch signaling is involved in microglial activation and subsequent inflammation-rel...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-018-2544-5
更新日期：2018-06-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00972490
更新日期：1988-04-01 00:00:00
abstract：:In a study of the role of taurine in the genesis of epilepsy the effects of metrazol-induced convulsions on the uptake and distribution of taurine in the brain were measured. In vivo we found no significant uptake of taurine in the mouse brain; in rabbit brain in most areas significant taurine uptake was found. The ph...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964640
更新日期：1979-08-01 00:00:00
abstract：:Gangliosides, the acidic glycosphingolipids (GSLs) containing N-acetylgalactosamine and sialic acid are ubiquitous in the central nervous system. At least six DSL-glycosyltransferase activities (GLTs Gangliosides, the acidic glycosphingolipids (GSLs) containing N-acetylgalactosamine and sialic acid (or NAc-Neuraminic ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-012-0762-9
更新日期：2012-06-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-015-1529-x
更新日期：2015-04-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964168
更新日期：1984-02-01 00:00:00
abstract：:It is known that the NMDA-R NR1 subunit is needed for the receptor activity and that under hypoxia the evolution toward apoptosis or neuronal survival depends on the balance NR2A/NR2B subunits. This paper analyzes the effect of acute hypoxia on the above mentioned subunits mRNAs during development. The mean percentage...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-010-0221-4
更新日期：2010-10-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-007-9474-y
更新日期：2008-03-01 00:00:00
abstract：:Pig brain extracts from both soluble and membrane fractions were found to contain potent inhibitors for GABA synthesizing enzyme, GAD, referred to as endogenous GAD inhibitors (EGIs) and for the binding of GABA agonist, muscimol, referred to as muscimol binding inhibitors (MBIs). EGIs and MBIs were first purified thro...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966871
更新日期：1992-01-01 00:00:00
abstract：:Spinal cord injury (SCI) is a functional impairment of the spinal cord caused by external forces, accompanied by limb movement disorders and permanent paralysis, which seriously lowers the life quality of SCI patients. Secondary injury caused by inflammation attenuated the therapeutic effects of SCI. Therefore, the ex...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-020-03211-y
更新日期：2021-01-13 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00963828
更新日期：1979-02-01 00:00:00
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journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965167
更新日期：1986-01-01 00:00:00
abstract：:Glutamate, the primary excitatory neurotransmitter in the central nervous system (CNS), exerts neuromodulatory actions via the activation of metabotropic glutamate (mGlu) receptors. There are eight known mGlu receptor subtypes (mGlu1-8), which are widely expressed throughout the brain, and are divided into three group...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-014-1415-y
更新日期：2014-10-01 00:00:00
abstract：:In the present study, we aimed to investigate the effects of pinealectomy and chronic melatonin administration on focal epileptiform activity induced by penicillin in the rat cortex and to determine the relation between melatonin levels and electrocorticogram (ECoG) power spectrum. For this purpose, male Sprague-Dawle...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-013-1069-1
更新日期：2013-08-01 00:00:00
abstract：:The effects of mu, delta, and kappa receptor-agonists on forskolin stimulated cyclic adenosine-3',5'-monophosphate (cAMP) formation were examined in astroglial enriched primary cultures from the cerebral cortex of newborn rats. Intracellular cAMP accumulation was quantified by radioimmunoassay. Morphine was used as a ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF01101714
更新日期：1990-11-01 00:00:00
abstract：:Cloned fragments of members of the Drosophila and mouse major heat shock (hsp70) gene family were used to demonstrate that homologous sequences are present in the rabbit genome. After a physiologically relevant increase in body temperature of 3 degrees C, transcription of inducible hsp70 genes is detected in both the ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964846
更新日期：1985-09-01 00:00:00
abstract：:Mutations in presenilin 1 (PS-1) are associated with most early-onset familiar Alzheimer's disease (AD). Previous studies have demonstrated that PS-1 mutations enhance the production of beta-amyloid (Aβ). In this study, we further examined the in vivo effects of PS-1 mutation on tau and synapse protein markers. The da...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-011-0575-2
更新日期：2012-01-01 00:00:00
abstract：:Recent evidence suggests that young rodents submitted to high fructose (FRU) diet develop metabolic, and cognitive dysfunctions. However, it remains unclear whether these detrimental effects of FRU intake can also be observed in middle-aged mice. Nine months-old C57BL/6 female mice were fed with water (Control) or 10%...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-020-03135-7
更新日期：2020-12-01 00:00:00
abstract：:In vitro aggregation and fibrillization of synthetic amyloid beta-protein Abeta 1-40 was assessed in the conditioned media from rhabdomyosarcoma (CRL 1598, HTB 82, HTB 153, CCL 136), adenocarcinoma (CCL 218), neuroblastoma (SY5Y), and COS cells cultured in the absence and presence of 10% heat-inactivated fetal bovine ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1027379926976
更新日期：1997-02-01 00:00:00
