Abstract：
:We have previously shown that angiotensin II (Ang II) stimulates astrocyte growth through activation of ERK1/2 mitogen activated protein (MAP) kinases. In the current study, we determined whether Ang II stimulates the expression of c-fos, c-jun and c-myc in brainstem astrocyte cultures. Reverse transcriptase-PCR analysis showed c-fos, c-jun, and c-myc mRNAs were induced by Ang II. The EC50 values for Ang II stimulation of c-fos, c-jun and c-myc were 1.3, 1.68 and 1.4 nM, respectively. Ang II (100 nM) induced peak stimulation for all genes by 45 min followed by a gradual decline. Inhibition of ERK1/2 by PD98059 attenuated Ang II-induced c-fos and c-myc mRNA expression (by 75% and 100%, respectively) but was ineffective in preventing Ang II induction of c-jun. These studies show for the first time in brainstem astrocytes that Ang II induces the expression of c-fos, c-myc and c-jun, and showed that ERK1/2 mediate Ang II stimulation of c-fos and c-myc. These data implicate the ERK1/2 MAP kinase pathway as a divergent point in controlling Ang II stimulation of immediate early response genes in the central nervous system.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Delaney J,Chiarello R,Villar D,Kandalam U,Castejon AM,Clark MAdoi
10.1007/s11064-007-9474-ysubject
Has Abstractpub_date
2008-03-01 00:00:00pages
545-50issue
3eissn
0364-3190issn
1573-6903journal_volume
33pub_type
杂志文章abstract：:cDNA microarray analysis of 1-methyl-4-phenyl-pyridinium (MPP+) toxicity (1 mM, 72 h) in undifferentiated SH-SY5Y cells identified 48 genes that displayed a signal intensity greater than the mean of all differentially expressed genes and a two-fold or greater difference in normalized expression. RT-PCR analysis of a s...
journal_title：Neurochemical research
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journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-017-2307-8
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abstract：:Multiple sclerosis (MS) is a demyelinating disease that affects the central nervous system. MS is the most common neurological disorder in young adults with a greater incidence among females. Male gonadal hormones have a protective effect on neural system development and myelin maturation. In this study, we investigat...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-011-0510-6
更新日期：2011-10-01 00:00:00
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abstract：: ...
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journal_title：Neurochemical research
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更新日期：2015-01-01 00:00:00
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journal_title：Neurochemical research
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journal_title：Neurochemical research
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更新日期：2019-02-01 00:00:00
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abstract：:The changes in both the levels of some free amino acids and their metabolism in the rat brain during the first 24 hr of postnatal life were studied. The content of glutamic acid decreased for the first 2 hr; it remained at the lowest level for the next 4 hr, when it began to increase. The content of alanine decreased ...
journal_title：Neurochemical research
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doi：10.1007/BF00966169
更新日期：1980-10-01 00:00:00
