Abstract:
:Proteolipid protein (PLP) is the major intrinsic membrane protein of CNS myelin and is expressed in oligodendrocytes as part of a coordinate program of myelin-specific gene activation. In order to identify the DNA sequences and proteins involved in the regulation of PLP transcription, we have analyzed the 5' flanking sequences of the rat PLP gene by transient transfections into primary cultures of developing oligodendrocytes, the glial tumor line, C6, and L cells. High levels of expression of the CAT reporter gene in oligodendrocytes and C6 cells were obtained with constructs containing both 4270 and 225 bp of PLP promoter. A fusion construct containing 1061 bp of the PLP promoter, however, showed two-fold lower CAT expression. In addition, the activity of these promoter fusion constructs in oligodendrocytes was 2.5-4.6 higher than that observed in C6 cells, while very little expression was found in L cells. These data suggest that 225 bp of PLP promoter is sufficient for oligodendrocyte-specific regulation of PLP expression. Furthermore, both positive and negative elements within the PLP promoter are involved in this process. Finally, primary cultures of developing oligodendrocytes are a useful model system for the analysis of myelin-specific gene activation.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Cambi F,Kamholz Jdoi
10.1007/BF00968716subject
Has Abstractpub_date
1994-08-01 00:00:00pages
1055-60issue
8eissn
0364-3190issn
1573-6903journal_volume
19pub_type
杂志文章abstract::We propose the following scheme for cerebral uptake and overall metabolism of glucose in vivo: that brain selects from two pools of glucose anomers in arterial blood, that it takes up excess glucose, that glucose enters the brain tissue as glucose-6-phosphate through the actions of mutarotase and hexokinase, that some...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964568
更新日期:1985-02-01 00:00:00
abstract::Experimental allergic encephalomyelitis (EAE) is an experimentally induced autoallergic demyelinating disease which is caused by immunization with a particular neuroantigen, such as myelin basic protein (MBP). Results have suggested that protease inhibitors might be useful therapeutically. Leupeptin (acetyl-L-leucyl-L...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964667
更新日期:1984-10-01 00:00:00
abstract::Post-ischemic changes in energy metabolites and natural antioxidant compounds have been measured in rat brain in vitro concurrent with two different assays for peroxidized lipids. No exogenous free radical initiators were employed. In vitro oxygenation of minced brain preparations for periods of 10 minutes to 4 hours,...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965496
更新日期:1982-04-01 00:00:00
abstract::Senescence of the central nervous system is characterized by a progressive loss of neurons that can result in physiological and behavioral impairments. Reduction in the levels of central neurotrophic factors or of neurotrophin receptors may be one of the causes of the onset of these degenerative events. Thus, a proper...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00995145
更新日期:1995-01-01 00:00:00
abstract::Chemokines are small secreted proteins that are essential for the recruitment and activation of specific leukocyte subsets at sites of inflammation and for the development and homeostasis of lymphoid and nonlymphoid tissues. During the past decade, chemokines and their receptors have also emerged as key signaling mole...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1023/b:nere.0000021246.96864.89
更新日期:2004-05-01 00:00:00
abstract::Exposure of guinea pig brain slices to low concentrations (10 microM) of NMDA caused decreases in PCr and ATP within 30 min, with a slower decrease in NAA and increase in lactate, both detectable after 1 h. Exposure to NMDA for over 1 h or at higher concentrations caused further increases in lactate and decreases in N...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1014898421330
更新日期:2002-02-01 00:00:00
abstract::The effect of postdecapitative ischemia (5 min at 37 degrees) and hypoxia (5% O2, 95% N2, 30 min) on the distribution of protein radioactivity in the cellular fractions of guinea pig cerebral cortex was studied. Ischemic conditions resulted in the increase of total radioactivity level and protein content in the mitoch...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00963828
更新日期:1979-02-01 00:00:00
abstract::In the unrestrained rat, the hyperphagic-like ingestion of food evoked by the sustained elevation of neuropeptide-Y (NPY) in the hypothalamus was correlated with the release and turnover of monoaminergic transmitters in this structure. A single guide tube was implanted stereotaxically in the perifornical region of the...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00967290
更新日期:1992-11-01 00:00:00
abstract::Tetrahydrobiopterin and the folate coenzymes can reciprocally interact in ways that would be useful to the metabolic pathways subserved by both of these coenzymes. Thus, through one of the reactions catalyzed by methylene tetrahydrofolate reductase, 5-CH3-H4-folate can regenerate BH4 from q-BH2 and q-BH2 can provide a...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/BF00965847
更新日期:1991-09-01 00:00:00
abstract::Chlorpyrifos (CPF), an organophosphorus insecticide is known to cause ill health in non-target animals by inducing oxidative stress. In this study influence of cold stress (15°C and 20°C) and age as modulating factors on CPF induced oxidative stress was addressed to assess age-related differences and vulnerability in ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-010-0311-3
更新日期:2011-02-01 00:00:00
abstract::Single exposure of adult male rats to low environmental temperature (LET, 12 +/- 0.5 degrees C) for 2 h significantly increased the hypothalamic and striatal GABA levels without affecting those in other regions of brain. The activity of glutamate decarboxylase (GAD) was elevated in hypothalamus (H) and corpus striatum...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00968559
更新日期:1990-08-01 00:00:00
abstract::Metabolism of triacylglycerol (TAG) in developing brain has been examined. TAG is a relatively minor fraction of brain lipid in both suckling and adult rats and cannot be accounted for as entrapped blood. When glycerol tri[1-14C]oleate and [2-3H]glycerol trioleate were simultaneously injected intracerebrally into suck...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00966679
更新日期:1981-11-01 00:00:00
abstract::Prion diseases are conformational diseases, many factors are involved in altering the conformation of prion, such as RNA, DNA, pH, and copper etc. However the neurotoxic mechanism of prion diseases is not clear yet. The aim of this study is to investigate the effect of the nucleoprotein complex of RNA and recombinant ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-011-0506-2
更新日期:2011-10-01 00:00:00
abstract::Hypoxia is one of the major common components of vascular risk factors for pathogenesis of Alzheimer's disease. This study investigated the possible relationship between hypoxia and alternative splicing of the excitatory amino acid transporter 2 (EAAT2) in a transgenic model for Alzheimer's disease. We used an APP23 m...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-007-9540-5
更新日期:2008-06-01 00:00:00
abstract::The influence of theophylline (2.5--100 mg/kg p.o.) on cyclic 3',5'-adenosine monophosphate (cAMP) and cyclic 3',5'-guanosine monophosphate (cGMP) in brain of Sprague-Dawley rats (0.5--3.0 hr after administration of theophylline) was investigated. It was found that theophylline increases cAMP and cGMP levels when admi...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964436
更新日期:1979-10-01 00:00:00
abstract::The effects of 100microg melatonin injection at postnatal day 5 (PD 5) on the development of the central serotonergic systems in male and female rats were investigated. The contents of serotonin (5-HT) and 5-hydroxy-3-indolacetic acid (5-HIAA) were measured in several extrahypothalamic regions at 3, 10 and 42 weeks of...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1007517524179
更新日期:2000-06-01 00:00:00
abstract::The oxidation of pyruvate is mediated by the pyruvate dehydrogenase complex (PDHC; EC 1.2.4.1, EC 2.3.1.12 and EC 1.6.4.3) whose catalytic activity is influenced by phosphorylation and by product inhibition. 2-Oxoglutarate and 3-hydroxybutyrate are readily utilized by brain mitochondria and inhibit pyruvate oxidation....
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00970527
更新日期:1987-08-01 00:00:00
abstract::The standard ELISA technique was improved for the detection of antigalactocerebroside antibody in biological fluid. Mouse monoclonal antigalactocerebroside antibody was used to demonstrate specificity and sensitivity of the technique. After optimization of the assay, the usefulness of this measurement for the evaluati...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00971533
更新日期:1988-03-01 00:00:00
abstract::Previous studies have shown that early life stress induced by maternal separation or non-handling can lead to behavioural deficits in rats and that these deficits can be alleviated by providing palatable cafeteria high-fat diet (HFD). In these studies we investigated the effects of maternal separation or non-handling ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-013-0985-4
更新日期:2013-04-01 00:00:00
abstract::The original version of this article unfortunately contained a mistake. The spelling of the author Tommaso Ianniti was incorrect and has been corrected as Tommaso Iannitti. The original article has been corrected. ...
journal_title:Neurochemical research
pub_type: 杂志文章,已发布勘误
doi:10.1007/s11064-018-2468-0
更新日期:2018-02-01 00:00:00
abstract::Antidepressive drugs offer considerable symptomatic relief in mood disorders and, although commonly discovered by screening with single biological targets, most interact with multiple receptors and signaling pathways. Antidepressants require a treatment regimen of several weeks before clinical efficacy is achieved in ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-005-8513-9
更新日期:2005-11-01 00:00:00
abstract::Brofaromine, a selective and reversible inhibitor of monoamine oxidase-A (MAO-A) was given to 19 women while 17 received placebo for 8 weeks. All met DSM III-R criteria for bulimia nervosa, a psychiatric disorder in which uncontrolled overeating episodes are accompanied by purging activities and extreme concerns about...
journal_title:Neurochemical research
pub_type: 临床试验,杂志文章
doi:10.1007/BF00975048
更新日期:1993-12-01 00:00:00
abstract::The overlapping distribution of opioid and cholecystokinin (CCK) peptides and their receptors (mu and delta opioid receptors; CCK-A and CCK-B receptors) in the central nervous system have led to a large number of studies aimed at clarifying the functional relationships between these two neuropeptides. Most of the phar...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/BF02532381
更新日期:1996-11-01 00:00:00
abstract::The copper-transporting ATPases Atp7A and Atp7B play a major role in controlling intracellular copper levels. In addition, they are believed to deliver copper to the copper-requiring proteins destined for the secretory vesicles. One cuproprotein, dopamine beta-hydroxylase (DBH) functions in the biosynthesis of norepin...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1023219308890
更新日期:2003-06-01 00:00:00
abstract::Sonic hedgehog (Shh), a secreted glycoprotein factor, can activate the Shh pathway, which has been implicated in neuronal polarization involving neurite outgrowth. However, little evidence is available about the effect of Shh on neurite outgrowth in primary cortical neurons and its potential mechanism. Here, we reveal...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-015-1736-5
更新日期:2016-04-01 00:00:00
abstract::The aim of the present study was to investigate possible membrane and genomic effects of corticosterone on the noradrenergic system of the rat brain. Corticosterone effects were studied in vivo by treating rats s.c. with 10 mg/kg corticosterone for 7 or 14 days. In the first two experiments corticosterone significantl...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00966670
更新日期:1992-03-01 00:00:00
abstract::Sandhoff disease is an incurable neurodegenerative disorder caused by mutations in the lysosomal hydrolase β-hexosaminidase. Deficiency in this enzyme leads to excessive accumulation of ganglioside GM2 and its asialo derivative, GA2, in brain and visceral tissues. Small molecule inhibitors of ceramide-specific glucosy...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-013-0992-5
更新日期:2013-04-01 00:00:00
abstract::The pineal gland is a neuroendocrine gland responsible for nocturnal synthesis of melatonin. During early development of the rodent pineal gland from the roof of the diencephalon, homeobox genes of the orthodenticle homeobox (Otx)- and paired box (Pax)-families are expressed and are essential for normal pineal develop...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-012-0906-y
更新日期:2013-06-01 00:00:00
abstract::Metal ions are often used to block calcium channels in various tissues, including synaptosomes. In the present study, Fura-2 was used to determine the effectiveness of various metal ions as calcium channel blockers in rat brain synaptosomes in vitro. In buffer solutions, La3+ and Cd2+ increased the Fura-2 fluorescence...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965611
更新日期:1989-11-01 00:00:00
abstract::In membranes of rat striatum, phorbol 12-myristate 13-acetate (PMA), a potent activator of Ca2+/phospholipid-dependent protein kinase, enhanced adenylate cyclase activity by counteracting the inhibition elicited by GTP. Exposure to pertussis toxin caused a similar alteration of the GTP-regulation of the enzyme activit...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF01101712
更新日期:1990-11-01 00:00:00