Abstract：
:Sonic hedgehog (Shh), a secreted glycoprotein factor, can activate the Shh pathway, which has been implicated in neuronal polarization involving neurite outgrowth. However, little evidence is available about the effect of Shh on neurite outgrowth in primary cortical neurons and its potential mechanism. Here, we revealed that Shh increased neurite outgrowth in primary cortical neurons, while the Shh pathway inhibitor (cyclopamine, CPM) partially suppressed Shh-induced neurite outgrowth. Similar results were found for the expressions of Shh and Patched genes in Shh-induced primary cortical neurons. Moreover, Shh increased the levels of brain-derived neurotrophic factor (BDNF) not only in lysates and in culture medium but also in the longest neurites of primary cortical neurons, which was partially blocked by CPM. In addition, blocking of BDNF action suppressed Shh-mediated neurite elongation in primary cortical neurons. In conclusion, these findings suggest that Shh promotes neurite outgrowth in primary cortical neurons at least partially through modulating BDNF expression.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
He W,Cui L,Zhang C,Zhang X,He J,Xie Ydoi
10.1007/s11064-015-1736-5subject
Has Abstractpub_date
2016-04-01 00:00:00pages
687-95issue
4eissn
0364-3190issn
1573-6903pii
10.1007/s11064-015-1736-5journal_volume
41pub_type
杂志文章abstract：:Using a model of middle cerebral artery occlusion (MCAO), we have previously demonstrated that treadmill exercise promotes angiogenesis in the ischemic penumbra through caveolin-1/VEGF signaling pathways. However, the function of caveolin-1/VEGF signaling in neurogenesis after MCAO has not been determined. In this stu...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-016-2081-z
更新日期：2017-02-01 00:00:00
abstract：:Glioblastoma (GBM) is the most aggressive and lethal form of brain tumor. However, therapeutic strategies against malignant gliomas have not been completely established. Runt-related transcription factor 2 (Runx2) is an essential gene for skeletal development but its regulatory role in the malignant progression of gli...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-018-2626-4
更新日期：2018-11-01 00:00:00
abstract：:The amyloid peptide (Abeta) deposited in Alzheimer's disease (AD) is generated by beta- and gamma-secretase processing of a larger integral membrane protein precursor (APP). Intramembrane processing of APP by gamma-secretase also yields an intracellular fragment, CTFgamma (a.k.a. AICD), which is highly conserved and i...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-007-9449-z
更新日期：2007-12-01 00:00:00
abstract：:Plasmalogens are ether-linked phospholipids that are abundant in nervous tissues. Their biological role is unclear, but may involve membrane structure/function and antioxidant activities. This study further investigates a recent report that chronic administration of myo-inositol in rats increased brain phosphatidyleth...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/b:nere.0000018859.23881.f9
更新日期：2004-04-01 00:00:00
abstract：:1-Methyl-4-phenylpyridinium (MPP(+)) or 6-hydroxydopamine (6-OHDA) caused a nuclear damage, the mitochondrial membrane permeability changes, leading to the cytochrome c release and caspase-3 activation, the formation of reactive oxygen species and the depletion of GSH in PC12 cells. Nicardipine (a calcium channel bloc...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-006-9088-9
更新日期：2006-07-01 00:00:00
abstract：:Berberine and palmatine exhibit a mild and competitive inhibition on bovine adrenal tyrosine hydroxylase (EC 1.14.16.2; TH). In this study, the inhibitory effects of protoberberine alkaloids (such as berberine, palmatine and coptisine) on dopamine biosynthesis in PC12 cells were investigated. Treatment with berberine ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1007541020736
更新日期：2000-03-01 00:00:00
abstract：:N-terminal protein arginylation has been demonstrated in vitro and in situ and has been reported to increase following injury to sciatic nerves of rats. The present study attempts to demonstrate these reactions in vivo by applying [3H]Arg to the cut end of sciatic nerves in anesthetized rats and assaying for N-termina...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1021998227237
更新日期：1997-12-01 00:00:00
abstract：:One pathway by which tryptophan is metabolized in the brain as well as in the periphery is through cleavage of the indole ring to formylkynurenine and then kynurenine. Indoleamine-2,3-dioxygenase, the enzyme that catalyzes this cleavage, and kynurenine are distributed all across the different anatomic regions of brain...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/BF00964611
更新日期：1980-03-01 00:00:00
abstract：:The activity of guanine deaminase (GAH, E.C.3.5.4.3) was lower in rat cerebellum soluble and microsomal fractions than in rat brain subfractions. Adenosine deaminase (ADA, E.C.3.5.4.4) activity was released in higher proportion than guanine deaminase, purine nucleoside phosphorylase (PNP, E.C.2.1.2.4), 5'-nucleotidase...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965016
更新日期：1986-03-01 00:00:00
abstract：:The mortality of patients with malignant gliomas remains high despite the advancement in multi-modal therapy including surgery, radio- and chemotherapy. Glioma stem cells (GSCs), sharing some characteristics with normal neural stem cells (NSCs), contribute to the cellular origin for primary gliomas and the recurrence ...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-012-0879-x
更新日期：2012-12-01 00:00:00
abstract：:Prion diseases are conformational diseases, many factors are involved in altering the conformation of prion, such as RNA, DNA, pH, and copper etc. However the neurotoxic mechanism of prion diseases is not clear yet. The aim of this study is to investigate the effect of the nucleoprotein complex of RNA and recombinant ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-011-0506-2
更新日期：2011-10-01 00:00:00
abstract：:Metformin is the most frequently used drug for the treatment of type 2 diabetes in humans. However, only little is known about effects of metformin on brain metabolism. To investigate potential metabolic consequences of an exposure of brain cells to metformin, we incubated rat astrocyte-rich primary cultures with this...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-015-1733-8
更新日期：2017-01-01 00:00:00
abstract：:Estrogen action is necessary for evidencing the stimulatory action of mifepristone and naloxone on prolactin (PRL) secretion during late pregnancy. Our aim is to determine the mechanism mediating this facilitator action of estrogens. To investigate the hypothalamic mechanisms involved in estrogen actions in PRL secret...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-016-2067-x
更新日期：2016-12-01 00:00:00
abstract：:Mevalonate phosphorylation was studied in neonatal chick brain. Formation of phosphorylated derivatives of mevalonic acid increased with the pH in the range assays (5.5-9.5). Phosphomevalonate kinase was completely inactivated after treatment at 50 degree C for 5-10 min, whereas mevalonate kinase was found to retain i...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965047
更新日期：1981-08-01 00:00:00
abstract：:Immunocytochemistry was used to determine sites of synthesis and pathways for the transport of the neuropeptide, Leucomyosuppressin (pQDVDHVFLRFamide) in the cockroach, Leucophaea maderae. This study led to identification of neurons in the brain and thoracic ganglia reactive to polyclonal antibodies raised against thi...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00974872
更新日期：1991-05-01 00:00:00
abstract：:miR-126 which is considered one of the most important miRNAs for maintaining vascular integrity, plays an important role in neuroprotection after cerebral ischemia-reperfusion (I-R). Moreover, vascular endothelial growth factor A (VEGFA), sprouty-related EVH1 domain-containing protein 1 (SPRED1), and Raf-1 are also in...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-020-02986-4
更新日期：2020-05-01 00:00:00
abstract：:Glutamate is the major excitatory neurotransmitter in the mammalian central nervous system (CNS) and exerts its actions via a number of ionotropic glutamate receptors/channels and metabotropic glutamate (mGlu) receptors. In addition to being expressed in neurons, glutamate receptors are expressed in different types of...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-008-9694-9
更新日期：2008-12-01 00:00:00
abstract：:We report here the isolation of fractions enriched in components of the myelin-like membranes surrounding the giant axons of the earthworm. Lumbricus terrestris L. The composition and purity of the fractions have been assessed using SDS-protein electrophoresis, Western immunoblots, and electron microscopy. Preliminary...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00970759
更新日期：1988-09-01 00:00:00
abstract：:Leucine is rapidly metabolized in astroglial primary cultures. Therefore, it is considered as valuable fuel in brain energy metabolism. Only few of the leucine metabolites generated and released by astroglial cells have been identified. Therefore, a more detailed study was performed analyzing by NMR techniques the 13C...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-004-6879-8
更新日期：2004-11-01 00:00:00
abstract：:The decline in neuronal function during aging may result from increases in extracellular glutamate (Glu), Glu-induced neurotoxicity, and altered mitochondrial metabolism. To study metabolic responses to persistently high levels of Glu at synapses during aging, we used transgenic (Tg) mice that over-express the enzyme ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-014-1239-9
更新日期：2014-01-01 00:00:00
abstract：:The interactions of S-nitrosoglutathione (GSNO) with the ionotropic glutamate receptors were studied on synaptic membranes isolated from the pig cerebral cortex. GSNO displaced the binding of [3H]glutamate, 3-[(R)-2-carboxypiperazin-4-yl] [3H]propyl-1-phosphonate ([3H]CPP), a competitive N-methyl-D-aspartate (NMDA) an...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1007626230278
更新日期：2000-08-01 00:00:00
abstract：:There is increasing evidence that proteins normally involved in the cell cycle play a role in the regulation of neuronal apoptotic death following various insults. However, it is not clear if the same mechanisms regulate cell death of oligodendrocytes as well. In this study, we investigated the mechanism of ceramide-i...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/b:nere.0000021236.32785.37
更新日期：2004-05-01 00:00:00
abstract：:Metabolic turnover of axonally transported glycoproteins has been examined in membranous and soluble subfractions of goldfish optic tectum following intraocular injection of [3H]fucose. Radioactivity in total transported glycoproteins reached a maximum in the tectum after 24-30 hr, then declined with a half-life of ap...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964224
更新日期：1980-04-01 00:00:00
abstract：:Amphiphile dependency, solubility in aqueous solutions, and sensitivity to proteolysis of acetylcholinesterase (AChE) and nonspecific cholinesterase (nsChE) in the rat superior cervical ganglion were studied and compared to properties of soluble plasma cholinesterases. Ganglion AChE shows strong amphiphile dependency:...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964537
更新日期：1985-06-01 00:00:00
abstract：:We recently presented evidence that distinct morphine and enkephalin receptors coexist in an opioid receptor complex (Mol. Pharmacol. 21:548-557, 1982). In this paper, we present data which demonstrate that in the presence of sodium leucine enkephalin noncompetitively inhibits the binding of [3H]naloxone to a crude pa...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964552
更新日期：1983-07-01 00:00:00
abstract：:Ketogenic diet (KD) has been shown to be beneficial in a range of neurological disorders, with ketone metabolite β-hydroxybutyrate (βOHB) reported to block the nucleotide oligomerization domain-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome in bone marrow-derived macrophages. In this study, we sh...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-020-03156-2
更新日期：2020-10-27 00:00:00
abstract：:The importance of astrocytic K(+) uptake for extracellular K(+) ([K(+)](e)) clearance during neuronal stimulation or pathophysiological conditions is increasingly acknowledged. It occurs by preferential stimulation of the astrocytic Na(+),K(+)-ATPase, which has higher K(m) and V(max) values than its neuronal counterpa...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-012-0938-3
更新日期：2013-03-01 00:00:00
abstract：:Since 1973, multiple effects of basic fibroblast growth factor have been described in a large number of cells. These effects include proliferation, survival and differentiation. The aim of this work was to study the intracellular pathways involved in the basic fibroblast growth factor (FGF2) effect on rat retinal cell...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-007-9491-x
更新日期：2008-05-01 00:00:00
abstract：:Caspase activation and apoptotic events may take place in terminal regions far removed from the cell body and contribute to synapse loss in neurodegenerative diseases. For examination of events in terminals, we have developed a cell-free assay using quantitative flow cytometric analysis (fluorescence-activated cell so...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1019840417796
更新日期：2002-06-01 00:00:00
abstract：:The modulation of intracellular GABA activated 36Cl- in-->out permeation across single Deiters' neuron membranes has been studied in a microchamber system. Addition of Mg2+/ATP on the membrane cytoplasmic side reduces strongly the GABA effect as does ATP alone. However, the greatest inhibition of the GABA effect is gi...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1021073230464
更新日期：1999-08-01 00:00:00
