Abstract：
:Since 1973, multiple effects of basic fibroblast growth factor have been described in a large number of cells. These effects include proliferation, survival and differentiation. The aim of this work was to study the intracellular pathways involved in the basic fibroblast growth factor (FGF2) effect on rat retinal cells proliferation in vitro. Our data show that treatment with FGF2 increases proliferation in a concentration- and time-dependent manner. The effect of 25 ng/ml FGF2 was blocked by 10 microM genistein, a tyrosine kinase inhibitor and by 25 microM LY294002, a PI3 kinase inhibitor. The concomitant treatment with 0.3 microM chelerythrine chloride, a protein kinase C inhibitor, and 6.25 microM LY294002 also inhibited the effect of FGF2. Our results suggest that the proliferative effect of FGF2 on retinal cell cultures involves the activation of distinct kinases.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Guilarducci-Ferraz CV,da Silva GM,Torres PM,Dos Santos AA,de Araújo EGdoi
10.1007/s11064-007-9491-xsubject
Has Abstractpub_date
2008-05-01 00:00:00pages
754-64issue
5eissn
0364-3190issn
1573-6903journal_volume
33pub_type
杂志文章abstract：:We have previously shown that extracellular ATP caused cell death in PC12 cells through activation of its receptors. Oxidative stress has been implicated as a mechanism of cell death caused by extracellular ATP. In the present study we examined the possible signal transduction cascades leading to cell death by extrace...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1022438703271
更新日期：1998-04-01 00:00:00
abstract：:Agonist occupancy of muscarinic cholinergic receptors in human SH-SY-5Y neuroblastoma cells elicited two kinetically distinct phases of phosphoinositide hydrolysis when monitored by either an increased mass of inositol 1,4,5-trisphosphate, or the accumulation of a total inositol phosphate fraction. Within 5s of the ad...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00971329
更新日期：1994-05-01 00:00:00
abstract：:It has been reported that central dopaminergic system is implicated in the mechanism underlying general anesthesia. Whether dopamine (DA) neurons in midbrain ventral periaqueductal gray (vPAG) are involved in general anesthesia and how general anesthetics affect these neurons remain sparsely documented. To determine t...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-018-2486-y
更新日期：2018-04-01 00:00:00
abstract：:Association of the candidate gene DLST with late-onset Alzheimer's disease (LOAD) risk has been suggested on the basis of case-control studies. This gene, located on chromosome 14q24.3, encodes a subunit of a mitochondrial component known to be defective in AD, the alpha-ketoglutarate dehydrogenase complex. Positive r...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/b:nere.0000014833.54481.1d
更新日期：2004-03-01 00:00:00
abstract：:There is growing evidence that ketone bodies (KB)-derived from fatty acid oxidation and produced during fasting or consumption of high-fat diets-can exert broad neuroprotective effects. With respect to epilepsy, KB (such as β-hydroxybutyrate or BHB, acetoacetate and acetone) have been shown to block acutely induced an...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-017-2253-5
更新日期：2017-07-01 00:00:00
abstract：:Convection enhanced delivery of 6-hydroxydopamine (6-OHDA) to the rat striatum results in a model of Parkinson's disease. An important feature of this unilateral model is the progressive loss of dopaminergic (DA) neurons over the course of several weeks. To improve the understanding of this model, gene expression chan...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-010-0133-3
更新日期：2010-05-01 00:00:00
abstract：:Brain noradrenergic system has been implicated in early-life stress effects on adult physiology and behavior; however, the mechanisms for this relationship are not clear. Here we tested the hypothesis that stress hormones, glucocorticoids, may affect noradrenergic system activity by modulating gene expression and func...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-011-0676-y
更新日期：2012-04-01 00:00:00
abstract：:1-Methyl-4-phenylpyridinium (MPP(+)) or 6-hydroxydopamine (6-OHDA) caused a nuclear damage, the mitochondrial membrane permeability changes, leading to the cytochrome c release and caspase-3 activation, the formation of reactive oxygen species and the depletion of GSH in PC12 cells. Nicardipine (a calcium channel bloc...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-006-9088-9
更新日期：2006-07-01 00:00:00
abstract：:Alzheimer's disease (AD) is the most common form of dementia in the aged population. Early-onset familial AD (FAD) involves mutations in a gene on chromosome 21 encoding the amyloid protein precursor or on chromosomes 14 or 1 encoding genes known as presenilins. All mutations examined have been found to increase the p...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1023/a:1022471729291
更新日期：1998-05-01 00:00:00
abstract：:Microvessels were isolated from a bovine cortex and the transport of glucose was investigated by using 2-deoxy-D-[3H]glucose (2-DG). The apparent Km for 2-DG transport was 118 microM and therefore indicates a significant high affinity for the substrate. The inhibition of 2-DG uptake by D-glucose showed an apparent Ki ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00963858
更新日期：1981-04-01 00:00:00
abstract：:We have previously reported that angiotensin receptor blockade reduces reperfusion hemorrhage in a suture occlusion model of stroke, despite increasing matrix metalloproteinase (MMP-9) activity. We hypothesized that candesartan will also decrease hemorrhage associated with delayed (6 h) tissue plasminogen activator (t...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-013-1185-y
更新日期：2013-12-01 00:00:00
abstract：:Analysis in mouse brain slices of the uptake of acetyl-L-[N-methyl-14C]carnitine with time showed it to be concentrative, and kinetic analysis gave a Km of 1.92 mM and a Vmax of 1.96 mumol/min per ml, indicating the presence of a low-affinity carrier system. The uptake was energy-requiring and sodium-dependent, being ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964865
更新日期：1989-05-01 00:00:00
abstract：:It was found that acute ethanol intoxication caused an imbalance of the neurotransmitters in the CNS: accumulation of GABA and serotonin and depletion of catecholamines. Alcohol depression was characterized by suppression of the evoked potentials of the various rat brain structures. Under chronic ethanol intoxication ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966062
更新日期：1982-11-01 00:00:00
abstract：:Mitochondrially bound brain hexokinase is solubilized by anesthetics and this effect has been suggested to contribute to anesthesia. In the present investigation the influence of the metabolic inhibitor 2-deoxy-D-glucose (2-DOG) was studied. An isolated rat brain preparation was used to avoid the contribution of perip...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965526
更新日期：1982-06-01 00:00:00
abstract：:Aging is accompanied by the loss of memory and cognitive functions. The extracellular signal-regulated kinase (ERK) pathway has been shown to play an essential role in synaptic plasticity and memory. Although a reduction in basal ERK1/2 activity has been found in the cerebral cortex in aged rats, changes in ERK1/2 mRN...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1024948532633
更新日期：2003-09-01 00:00:00
abstract：:When excised goldfish spinal cord is kept in physiological saline at room temperature, the myelin multilayers swell. As studied by X-ray diffraction, the original repeating distance of 150 A (AS myelin) swells to 177 A (AL myelin); no intermediate distances are seen. At least 70% of the myelin can undergo this gradual...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965941
更新日期：1986-08-01 00:00:00
abstract：:Eclalbasaponin II derived from Eclipta prostrata L. (Asteraceae) has been reported to have anti-fibrotic, anti-bacterial and autophagic activities, but its effect on cognitive function has not been investigated. We studied the effect of eclalbasaponin II on cholinergic blockade-induced memory impairment in mice using ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-017-2430-6
更新日期：2018-02-01 00:00:00
abstract：:The aim of this study was to determine the anticancer potential of Leonurus sibiricus extract derived from in vitro transgenic roots transformed by Agrobacetrium rhizogenes with AtPAP1 transcriptional factor, and that of transformed roots without construct, on grade IV human glioma cells and the U87MG cell line, and a...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-018-2551-6
更新日期：2018-07-01 00:00:00
abstract：:Complementary DNA (cDNA) clones containing the entire coding sequence for Diazepam Binding Inhibitor (DBI) peptide, a 10-kDa precursor of putative natural ligands of benzodiazepine recognition sites, were isolated from rat, human and cow libraries. The sequence of all these clones is highly conserved; however, the N-t...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/BF00972202
更新日期：1990-02-01 00:00:00
abstract：:Glioblastoma (GBM) is the most aggressive and lethal form of brain tumor. However, therapeutic strategies against malignant gliomas have not been completely established. Runt-related transcription factor 2 (Runx2) is an essential gene for skeletal development but its regulatory role in the malignant progression of gli...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-018-2626-4
更新日期：2018-11-01 00:00:00
abstract：:This study aimed at comparing the binding characteristics of [3H]ketanserin, a high-affinity serotonin 2A (5-HT2A) receptor antagonist, in the prefrontal cortex, hippocampus and striatum of human brain post-mortem. The results indicated the presence of a single population of binding sites in all the regions investigat...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1027366413289
更新日期：1997-06-01 00:00:00
abstract：:Pathogens such as bacterial lipopolysaccharide (LPS) play an important role in promoting the production of the inflammatory cytokines interleukin-1 beta (IL-1β) and tumour necrosis factor-α (TNF-α) in response to infection or damage in microglia. However, whether different signalling pathways regulate these two inflam...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-019-02895-1
更新日期：2019-12-01 00:00:00
abstract：:Calcium-naive synaptosomes were used to assess the effects of divalent cations on [3H]acetylcholine release from rat hippocampal homogenates. Following equilibration with calcium-free buffer (containing 10 microM EGTA), calcium reversibly increased [3H]acetylcholine efflux (up to five-fold) while causing no measurable...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966598
更新日期：1991-10-01 00:00:00
abstract：:Cognitive impairment in diabetes (CID) is a severe chronic complication of diabetes mellitus (DM). It has been hypothesized that diabetes can lead to cognitive dysfunction due to expression changes of excitatory neurotransmission mediated by N-methyl-D-aspartate receptors (NMDAR) and α-amino-3-hydroxy-5-methyl-4-isoxa...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-019-02733-4
更新日期：2019-04-01 00:00:00
abstract：:A simple, rapid method for determining depolarization-induced 45Ca influx into synaptosomes is described. Synaptosomes which had been depolarized in the presence of 45Ca were applied to a small column of Chelex-100 resin to separate free Ca2+ from that taken up by the tissue. Approximately 70% of the synaptosomal prot...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965062
更新日期：1982-02-01 00:00:00
abstract：:Apoptotic cell death pathways have been implicated in acute brain injuries, including cerebral ischemia, brain trauma, and spinal cord injury, and in chronic neurodegenerative diseases. Experimental ischemia and reperfusion models, such as transient focal/global ischemia in rodents, have been thoroughly studied and su...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-004-6869-x
更新日期：2004-11-01 00:00:00
abstract：:Oxidation of [2,3-14C]succinate in the intramitochondrial Krebs cycle was used as a probe to investigate the effect of ammonia on protein incorporation and Krebs cycle oxidation of succinate carbons in isolated rat hepatocytes. At low concentrations of ammonium chloride (0.1 to 0.5 mM) a slight increase in 14CO2 forma...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965690
更新日期：1991-07-01 00:00:00
abstract：:During the past decade a new approach to pathogenetic studies of hepatic encephalopathy has been undertaken to identify the neurochemical alterations which characterize the syndrome. Using animal models of hepatic encephalopathy electrophysiological, behavioral, pharmacological and biochemical evidence were provided o...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/BF00972205
更新日期：1990-02-01 00:00:00
abstract：:Sandhoff disease is an incurable neurodegenerative disorder caused by mutations in the lysosomal hydrolase β-hexosaminidase. Deficiency in this enzyme leads to excessive accumulation of ganglioside GM2 and its asialo derivative, GA2, in brain and visceral tissues. Small molecule inhibitors of ceramide-specific glucosy...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-013-0992-5
更新日期：2013-04-01 00:00:00
abstract：:In the present study, we aimed to investigate the effects of pinealectomy and chronic melatonin administration on focal epileptiform activity induced by penicillin in the rat cortex and to determine the relation between melatonin levels and electrocorticogram (ECoG) power spectrum. For this purpose, male Sprague-Dawle...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-013-1069-1
更新日期：2013-08-01 00:00:00
