Abstract：
:Complementary DNA (cDNA) clones containing the entire coding sequence for Diazepam Binding Inhibitor (DBI) peptide, a 10-kDa precursor of putative natural ligands of benzodiazepine recognition sites, were isolated from rat, human and cow libraries. The sequence of all these clones is highly conserved; however, the N-terminal sequence predicted by the human DBI clone differed from that of the other two clones. DBI cDNA, utilized as hybridization probe in Southern blot analysis, revealed that DBI of both human and rat might be encoded by a multiple family of 4-6 genes. Furthermore, we have used in situ chromosomes hybridization to map human DBI genes. The results indicate that a human DBI gene is localized on chromosome 2 and that three of the four hybridization signals detected by the human DBI probe are located on three other chromosomes. These findings raise a question as whether multiple DBI genes encode for different molecular forms of DBI. In the attempt to test this hypothesis, cow cDNA and human genomic libraries were screened with DBI cDNA. In this paper I report the isolation of clones from these libraries which, although hybridizing well to DBI cDNA, possess a low percentage of homology (46.7%), randomly distributed within the coding region of DBI cDNA. Whether or not these clones encode for peptides sharing the same physiological role as DBI is under investigation.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Mocchetti Idoi
10.1007/BF00972202subject
Has Abstractpub_date
1990-02-01 00:00:00pages
125-30issue
2eissn
0364-3190issn
1573-6903journal_volume
15pub_type
杂志文章,评审abstract：:A panel of mouse monoclonal antibodies to rat and human myelin-associated glycoprotein (MAG) was developed. Normal mice were unresponsive to rat MAG, and successful immunization with rat MAG was only achieved in autoimmune NZB mice. By contrast, all strains of mice were responsive to human MAG. The monoclonal antibodi...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964654
更新日期：1985-04-01 00:00:00
abstract：:More than a decade has passed since Zic family zinc finger proteins were discovered to be transcription factors controlling neuroectodermal differentiation (neural induction) in Xenopus laevis embryos. Although BMP-signal blocking has been shown to be a major upregulator of Zic genes in neuroectodermal differentiation...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-011-0422-5
更新日期：2011-07-01 00:00:00
abstract：:cDNA microarray analysis of 1-methyl-4-phenyl-pyridinium (MPP+) toxicity (1 mM, 72 h) in undifferentiated SH-SY5Y cells identified 48 genes that displayed a signal intensity greater than the mean of all differentially expressed genes and a two-fold or greater difference in normalized expression. RT-PCR analysis of a s...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1026179926780
更新日期：2003-12-01 00:00:00
abstract：:The anterograde axonal transport of choline-phosphoglycerides was studied in sciatic nerve motoneurons of adult (3-month-old) and aged (24-month-old) rats. After the spinal cord injection of [2-3H]glycerol, choline-phosphoglycerides; the major phospholipid class was transported along the nerve. The axonal transport ra...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00971365
更新日期：1987-01-01 00:00:00
abstract：:In order to develop a simplified method for long-term primary culture of highly-pure rat embryonic hippocampal neurons of low-density (10(3) cells/cm(2)), we optimized and modified conventional culturing methods. The modifications of our simplified method include: (1) combinational application of two growth substrates...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-010-0189-0
更新日期：2010-09-01 00:00:00
abstract：:Neuroprotectin D1 (NPD1), a docosahexaenoic acid (DHA)-derived lipid mediator, promotes survival in cells exposed to oxidative stress by inducing the activity of anti-inflammatory mediators and suppressing the expression of pro-inflammatory genes. Though retinal pigment epithelial (RPE) cells naturally produce NPD1 fr...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-010-0351-8
更新日期：2010-12-01 00:00:00
abstract：:Cholinergic muscarinic receptors, when stimulated by arecoline, can activate cytosolic phospholipase A(2) (cPLA(2)) to release arachidonic acid (AA) from membrane phospholipid. This signal can be imaged in the brain in vivo using quantitative autoradiography following the intravenous injection of radiolabeled AA, as a...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-007-9372-3
更新日期：2007-11-01 00:00:00
abstract：:The study provides detailed biochemical correlates to the common histopathological diagnoses in epilepsy. A dot immunobinding procedure was used for quantification of NSE, GFA, S-100, NCAM, NF 68 and NF 200. The material consisted of samples from 48 patients either selected for surgical treatment of partial epilepsy o...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00967255
更新日期：1993-04-01 00:00:00
abstract：:Direct evidence for the excitotoxicity of 3-N-oxalyl-L-alpha,beta-diaminopropionic acid (ODAP), the Lathyrus sativus neurotoxin has been studied by examining the binding of chemically synthesized [2,3 3H]ODAP ([3H]ODAP) to synaptic membranes. [3H]ODAP binding to membranes was mostly nonspecific, with only a very low s...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1020782119057
更新日期：1998-09-01 00:00:00
abstract：:The neurotrophins brain derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) are both expressed in developing cerebellum in addition to their tyrosine kinase receptors. TrkB and TrkC. In contrast to BDNF.NT-3 has only a negligible or a transient survival activity on cultured cerebellar granule neurons. The gra...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF02532309
更新日期：1996-07-01 00:00:00
abstract：:The cellular effects of eleven compounds including chalcone glycosides isolated from Brassica rapa L. 'hidabeni' and their synthetic derivatives were studied in rat pheochromocytoma PC12 cells. Of the compounds tested, 4'-O-β-D-glucopyranosyl-3',4-dimethoxychalcone (A2) significantly increased the levels of the phosph...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-013-1152-7
更新日期：2013-11-01 00:00:00
abstract：:Astrocytes have been considered to be transformed from radial glial cells that appear at early stage of development and play a scaffold-role for neuronal cell migration. Recent studies indicate that neuroepithelial cells in the spinal cord also give rise to astrocytes. However, the mode of astroglial generation and mi...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-008-9590-3
更新日期：2008-07-01 00:00:00
abstract：:As is known to all, neuroinflammation plays a vital role in early brain injury pathogenesis following subarachnoid hemorrhage (SAH). It has been shown that rutin have a property of inhibiting inflammation in many kinds of animal models. However, the effect of rutin on neuroinflammation after SAH remains uninvestigated...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-016-1863-7
更新日期：2016-06-01 00:00:00
abstract：:Most inborn errors of tyrosine catabolism produce hypertyrosinemia. Neurological manifestations are variable and some patients are developmentally normal, while others show different degrees of developmental retardation. Considering that current data do not eliminate the possibility that elevated levels of tyrosine an...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-013-1078-0
更新日期：2013-08-01 00:00:00
abstract：:The effects of pre-treatment with ENA-713, an acetylcholinesterase (AChE) inhibitor, on changes in pre- and postsynaptic cholinergic indices in gerbil brain following transient ischemia were studied at 4 and 14 days after recirculation. In the ischemic group, hippocampal acetylcholine (ACh) level was significantly red...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966804
更新日期：1994-02-01 00:00:00
abstract：:There is increasing evidence that proteins normally involved in the cell cycle play a role in the regulation of neuronal apoptotic death following various insults. However, it is not clear if the same mechanisms regulate cell death of oligodendrocytes as well. In this study, we investigated the mechanism of ceramide-i...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/b:nere.0000021236.32785.37
更新日期：2004-05-01 00:00:00
abstract：:Endoplasmic reticulum (ER) and mitochondria are intracellular organelles and their interactions are directly involved in different processes such as Ca(2+) signaling in cell survival and death mechanisms. Bcl-2 is an anti-apoptotic protein intrinsically related to ER and mitochondria, modulating Ca(2+) content in thes...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-011-0600-5
更新日期：2012-02-01 00:00:00
abstract：:Although nitric oxide (NO) plays key signaling roles in the nervous systems, excess NO leads to cell death. In this study, the involvement of p38 mitogen-activated protein kinase (p38 MAPK) and apoptosis signal-regulating kinase-1 (ASK1) in NO-induced cell death was investigated in PC12 cells. NO donor transiently act...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1010917129951
更新日期：2001-05-01 00:00:00
abstract：:Experimental allergic encephalomyelitis (EAE) is an experimentally induced autoallergic demyelinating disease which is caused by immunization with a particular neuroantigen, such as myelin basic protein (MBP). Results have suggested that protease inhibitors might be useful therapeutically. Leupeptin (acetyl-L-leucyl-L...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964667
更新日期：1984-10-01 00:00:00
abstract：:Metabotropic glutamate receptors (mGluR) play a role in synaptic transmission, neuronal modulation and plasticity but their action in epileptic activity is still controversial. On the other hand adenosine acts as a neuromodulator with endogenous anticonvulsive properties. Since cerebellum from epileptic patients has s...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-006-9275-8
更新日期：2007-07-01 00:00:00
abstract：:Danhong injection, a Chinese Materia Medica standardized product extracted from Radix Salviae miltiorrhizae and Flos Carthami tinctorii, is used extensively for the treatment of cerebrovascular diseases such as acutely cerebral infarction in clinic. In this study, we further investigated the mechanisms of Danhong inje...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-014-1384-1
更新日期：2014-09-01 00:00:00
abstract：:Gelsolin is an actin regulatory protein that generally distributed in a wide variety of body tissues, especially the brain tissues and cerebrospinal fluid. In this study we found that lumbar CSF-gelsolin concentrations markedly decreased in epileptic patients by enzyme linked immunosorbent assay. In order to help judg...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-011-0549-4
更新日期：2011-12-01 00:00:00
abstract：:The conversion of phosphoethanolamine to phosphocholine requires 3 separate N-methyltransferases. We had previously purified the enzyme catalyzing the last methylation, phosphodimethylethanolamine N-methyltransferase. We have successfully purified the enzyme catalyzing the initial methylation of phosphoethanolamine. A...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00995388
更新日期：1995-10-01 00:00:00
abstract：:Due to the biological importance of sodium and its relative scarcity within many natural environments, 'salt appetite' has evolved whereby dietary salt is highly sought after and palatable when tasted. In addition to peripheral responses, salt depletion is detected within the brain via circumventricular organs and 11β...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-017-2336-3
更新日期：2018-01-01 00:00:00
abstract：:The role of protein phosphorylation in catecholamine secretion from bovine adrenomedullary chromaffin cells was studied using different protein kinase inhibitors. Naphthalenesulfonamide derivatives as ML9 and ML7, more specific for the myosin light chain kinase, and the calmodulin antagonist W7 inhibited catecholamine...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00969089
更新日期：1993-03-01 00:00:00
abstract：:Pathophysiological concentrations of branched chain keto-acids (BCKAs), such as those that occur in maple syrup urine disease, inhibit oxygen consumption in liver homogenates and brain slices and the enzymatic activity of alpha-ketoglutarate- and pyruvate dehydrogenase complexes. Consistent with previous work, studies...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-007-9291-3
更新日期：2007-04-01 00:00:00
abstract：:The synthetic Liver X receptor (LXR) activator T0901317 has been reported to exert neuroprotective effect in Alzheimer's disease, but the relationship between LXR activation and beta-site amyloid precursor protein cleaving enzyme 1 (BACE-1) remains uncertain. This study investigated the effect of T0901317 on membrane ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-011-0513-3
更新日期：2011-10-01 00:00:00
abstract：:It is well known that antioxidants containing sulfhydryl (-SH) groups are protective against the toxic effects of mercury. The current study was designed to elucidate the mechanism(s) of the cytoprotective effects of glutathione (GSH) and N-acetylcysteine (NAC) against the toxicity of inorganic mercury (HgCl(2)) in ne...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-009-9962-3
更新日期：2009-09-01 00:00:00
abstract：:"Kindling" is a phenomenon of epileptogenesis, which has been widely used as an experimental model of temporal lobe epilepsy. At the present work we investigated the contribution of NMDA receptors in the Pentylenetetrazol-induced "kindling" model in the mouse brain, by using quantitative autoradiography and the radioa...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1021143813935
更新日期：1999-12-01 00:00:00
abstract：:Methylguanidine (MG) is known as not only a nephrotoxin but also as a neurotoxine. We have previously showed that MG itself generates hydroxyl radicals (*OH) in an in vitro study. In this study, we examined the inhibitory effects of ascorbate, EPC-K(1) (alpha-tocopheryl-L-ascorbate-2-O-phosphate diester), Trolox (wate...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-008-9811-9
更新日期：2009-04-01 00:00:00
