Abstract：
:Endoplasmic reticulum (ER) and mitochondria are intracellular organelles and their interactions are directly involved in different processes such as Ca(2+) signaling in cell survival and death mechanisms. Bcl-2 is an anti-apoptotic protein intrinsically related to ER and mitochondria, modulating Ca(2+) content in these organelles. We investigated the effects of Bcl-2 overexpression on ER and mitochondrial Ca(2+) dynamics in PC12 cells. Bcl-2 overexpressing and control cells were loaded with Fura 2/AM and stimulated with different drugs. Results showed that in Bcl-2 cells, ACh induced a lower Ca(2+) response compared to control. Ca(2+) release induced by TG was decreased in Bcl-2 cells, however, it was greater in Caff induced Ca(2+) rise. In addition, FCCP induced a higher Ca(2+) release in Bcl-2 cells. These results suggest that Bcl-2 overexpression modulate the ER Ca(2+) pools differently and the release of ER Ca(2+) may increase mitochondrial Ca(2+) accumulation. These alterations of intracellular Ca(2+) stores are important mechanisms for the control of Ca(2+) signaling.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Hirata H,Lopes GS,Jurkiewicz A,Garcez-do-Carmo L,Smaili SSdoi
10.1007/s11064-011-0600-5subject
Has Abstractpub_date
2012-02-01 00:00:00pages
238-43issue
2eissn
0364-3190issn
1573-6903journal_volume
37pub_type
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