Abstract:
:Treatment of neural membranes from rat cerebral cortex with phospholipase C (phosphatidylcholine cholinephosphohydrolase) inhibited the binding of radiolabelled antagonists to muscarinic acetylcholine receptors. This inhibition was incomplete, was not competitive, and did not appear to be related to the production of inhibitory products. The affinity of carbamylcholine for cortex muscarinic receptors was increased by phospholipase C action. The distribution of receptors between states of high and low affinity was not affected by phospholipase C; rather, the affinity for carbamylcholine of the lowest affinity receptors was selectively increased. This suggests that membrane lipids influence the interaction of the receptor binding subunit with other structures in the synaptic membrane.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Parthasarathy N,Pickard C,Aronstam RSdoi
10.1007/BF00964517subject
Has Abstractpub_date
1984-05-01 00:00:00pages
709-17issue
5eissn
0364-3190issn
1573-6903journal_volume
9pub_type
杂志文章abstract::Cerebral deposition of amyloid β-peptide (Aβ), a fundamental feature of Alzheimer's disease (AD), damages the neurocytes and impairs the cognition functions and associative learning memory of AD patients. A series of novel 2-arylethenylquinoline derivatives were synthesized and evaluated in our previous study, which i...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-017-2339-0
更新日期:2017-11-01 00:00:00
abstract::The present study addressed the hypothesis that the DA transporter ligand, [3H]mazindol, labels multiple sites/states associated with the dopamine (DA) transporter in striatal membranes. Incubations with [3H]mazindol proceeded for 18-24 hr at 4 degrees C in 55.2 mM sodium phosphate buffer, pH 7.4, with a protease inhi...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00966817
更新日期:1994-02-01 00:00:00
abstract::Thiamine deficiency (TD) produces severe neurodegenerative lesions. Studies have suggested that primary neurodegenerative events are associated with both oxidative stress and inflammation. Very little is known about the downstream effects on intracellular signaling pathways involved in neuronal death. The primary aim ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-020-02975-7
更新日期:2020-04-01 00:00:00
abstract::Subunit-specific antibodies to all the gamma subunit isoforms described in mammalian brain (gamma(1), gamma(2S), gamma(2L), and gamma(3) have been made. The proportion of GABA(A) receptors containing each gamma subunit isoform in various brain regions has been determined by quantitative immunoprecipitation. In all tes...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF02529132
更新日期:1996-02-01 00:00:00
abstract::The mechanism of focal demyelination in multiple sclerosis has been a long-standing enigma of this disorder. Cytokines, a diverse family of signalling molecules, are viewed as potential mediators of the process based on clinical observations and studies with animal models and tissue/cell culture systems. Myelin and ol...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1023/a:1022493013904
更新日期:1998-03-01 00:00:00
abstract::Vascular endothelial growth factor (VEGF) is a promising biological marker and prognostic indicator in many neurological diseases. Although VEGF concentrations in plasma and cerebrospinal fluid (CSF) are increasingly reported, CSF-VEGF stability pre- and during-assay procedures is seldom evaluated. In the current stud...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-011-0517-z
更新日期:2011-11-01 00:00:00
abstract::The monoclonal antibody 14E immunocytochemically stains the nuclear membrane of oligodendrocytes but not myelin in tissue sections of adult normal human white matter. The nuclear membranes of Schwann cells in human peripheral nerve and cerebellar Bergmann glia were also visualized with this antibody. In actively demye...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00993270
更新日期:1992-09-01 00:00:00
abstract::Aneurysmal subarachnoid hemorrhage still has a high mortality and morbidity despite notable advances in surgical approaches to cerebral aneurysm (CA). We examined the role of aspirin in vascular inflammation and degeneration. CA was induced in male Sprague-Dawley rats by ligating left common carotid artery and bilater...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-015-1603-4
更新日期:2015-07-01 00:00:00
abstract::Cyclin-dependent kinase 5 (Cdk5) plays a pivotal role in neuronal migration and differentiation, and in axonal elongation. Although many studies have been conducted to analyze neuronal functions of Cdk5, its kinase activity has also been reported during oligodendrocyte differentiation, which suggests Cdk5 may play an ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-010-0391-0
更新日期:2011-07-01 00:00:00
abstract::Since 1973, multiple effects of basic fibroblast growth factor have been described in a large number of cells. These effects include proliferation, survival and differentiation. The aim of this work was to study the intracellular pathways involved in the basic fibroblast growth factor (FGF2) effect on rat retinal cell...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-007-9491-x
更新日期:2008-05-01 00:00:00
abstract::The control and maintenance of the intracellular redox state is an essential task for cells and organisms. NAD(+) and NADH constitute a redox pair crucially involved in cellular metabolism as a cofactor for many dehydrogenases. In addition, NAD(+) is used as a substrate independent of its redox-carrier function by enz...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-012-0760-y
更新日期:2012-11-01 00:00:00
abstract::The cyclic GMP analog 8-bromo-cyclic GMP (8-Br-cGMP) protects differentiated murine oligodendrocytes (OLs) from caspase-mediated death initiated by staurosporine, thapsigargin or kainate. Caspase-independent death caused by high levels of NO is also partially prevented by 8-Br-cGMP. Inhibitors of protein kinase G (cGM...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-006-9187-7
更新日期:2007-02-01 00:00:00
abstract::We examined the role of hippocampal metabotropic glutamate receptor 5 (mGlu5) in spatial learning and memory. Although it has been shown that mGlu5 signalling is required for certain forms of learning and memory, its role in spatial learning is unclear since studies using pharmacological or knockout mice models provid...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-015-1595-0
更新日期:2015-06-01 00:00:00
abstract::In the present study, we evaluated the expression of some proliferation and differentiation markers in 15 DIV astrocyte cultures pretreated or not with 0.5 mM glutamate for 24 h and than maintained under chronic or acute treatment with 50 μM R(+)enantiomer or raceme alpha-lipoic acid (ALA). GFAP expression significant...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-010-0256-6
更新日期:2010-12-01 00:00:00
abstract::Lipid synthesis from acetoacetate and 3-hydroxybutyrate was studied in chick embryo from 15 to 21 days and in chick neonate from 1 to 21 days. Embryonic spinal cord showed higher ability than brain to incorporate acetoacetate into total lipids, although a sharp decrease was found at hatching. 3-Hydroxybutyrate incorpo...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00966692
更新日期:1993-10-01 00:00:00
abstract::The effects of graded doses of D-pipecolic acid (0.005-5 micrograms/animals s.c.) on tolerance to the hypothermic effect of ethanol (4 g/kg i.p.) were investigated in mice. D-Pipecolic acid itself did not change the core temperature or the acute hypothermic response to a single dose of ethanol. Repeated D-pipecolic ac...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00967746
更新日期:1986-12-01 00:00:00
abstract::Neuronal perikarya were isolated, using bulk preparative procedures, from bovine brains. Synaptosomes, neuronal perikarya, and brain homogenates had similar ganglioside patterns, with the synaptosomes containing at least four times more total ganglioside per mg protein than the neuronal perikarya and twice that of the...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00966108
更新日期:1976-04-01 00:00:00
abstract::Nitric oxide has been strongly implicated in the development of tissue infarction in response to focal cerebral ischemia. Nitric oxide and its derivatives can inhibit components of the electron transport chain, providing a likely target for these substances in ischemic and post-ischemic brain. Lactate content is incre...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-006-9160-5
更新日期:2007-04-01 00:00:00
abstract::Rabbits sensitized with whole nervous tissue or myelin basic protein (MBP) plus adjuvant and developing experimental allergic encephalomyelitis (EAE) were studied for the presence of oligoclonal immunoglobulin (Ig) bands in spinal fluid and serum. Samples obtained prior to sensitization and at the time of sacrifice we...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00963909
更新日期:1981-01-01 00:00:00
abstract::Astrocytes transport the monocarboxylate acetate, but synaptosomes do not. The reason for this is unknown, because both preparations express monocarboxylate transporters (MCT). The transport and metabolism of lactate, another monocarboxylate, was examined in these two preparations, and the results were compared to tho...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/b:nere.0000010450.21586.a6
更新日期:2004-01-01 00:00:00
abstract::Experimental evidence has suggested that the high endogenous levels of taurine found in the rat heart and retina are maintained to a large extent by transport processes out of the blood, rather than by endogenous biosynthesis. When these high levels are depleted, dysfunction ensues. In vitro studies have shown that be...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00970775
更新日期:1988-10-01 00:00:00
abstract::The regional distribution of 2-amino-4-phosphonobutyrate (APB)/chloride-insensitive L-[3H]glutamate binding sites in the rat central nervous system was compared with that of APB/chloride-sensitive and with sodium-dependent binding sites. The distribution of APB-sensitive and APB-insensitive sites was not correlated, b...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00963986
更新日期:1984-03-01 00:00:00
abstract::Folic acid plays an important role in neuroplasticity and acts as a neuroprotective agent, as observed in experimental brain ischemia studies. The aim of this study was to investigate the effects of folic acid on locomotor activity, aversive memory and Na(+),K(+)-ATPase activity in the frontal cortex and striatum in a...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-012-0757-6
更新日期:2012-08-01 00:00:00
abstract::Alpha adrenergic receptor subtypes in rat hippocampal membranes were studied, using [3H] clonidine as the radioactive ligand. On the basis of competitive binding studies, using the selective antagonist-prazosin, WB-4101, and yohimbine, [3H] clonidine appeared to bind to a population of presynaptic sites that are pharm...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965058
更新日期:1982-02-01 00:00:00
abstract::The effects of phenylalanine (PHE) and its deaminated metabolites phenylpyruvate (PHP), phenyllactate (PHL) and phenylacetate (PHA) on sodium and potassium activated adenosinetriphosphatase (Na+,K+-ATPase) in synaptosomes from rat brain were investigated. At very low concentrations (5-10 microM). PHE, PHL and PHA inhi...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965524
更新日期:1982-06-01 00:00:00
abstract::This study examined the relationship between the distance of axotomy and the death of injured retinal ganglion cells (RGCs) in adult hamsters and the relationship of cytochrome c and caspase-3 on the death pathway of RGCs. The left optic nerve (ON) of adult hamsters was transected either at 1 or 3 mm away from the opt...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-004-6889-6
更新日期:2004-11-01 00:00:00
abstract::The volume-regulated anion channel, VRAC, plays an important role in cell volume regulation. This channel is permeable for a wide variety of anions, amino acids, and organic osmolytes, including taurine. However, nothing is known about possible water permeability of this channel. Water permeability of endothelial cell...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/b:nere.0000010430.23137.be
更新日期:2004-01-01 00:00:00
abstract::Anesthetics such as propofol can provide neuroprotective effects against cerebral ischemia. However, the underlying mechanism of this beneficial effect is not clear. Therefore, we subjected male Sprague-Dawley rats to 2 h of middle cerebral artery occlusion and investigated how post-ischemic administration of propofol...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-012-0945-4
更新日期:2013-03-01 00:00:00
abstract::Berberine and palmatine exhibit a mild and competitive inhibition on bovine adrenal tyrosine hydroxylase (EC 1.14.16.2; TH). In this study, the inhibitory effects of protoberberine alkaloids (such as berberine, palmatine and coptisine) on dopamine biosynthesis in PC12 cells were investigated. Treatment with berberine ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1007541020736
更新日期:2000-03-01 00:00:00
abstract::The steady-state mRNA levels of NGF, BDNF and NT-3, and the mRNA levels of their receptors p75NGFR, trk, trkB, and trkC were examined in various human peripheral neuropathies, to determine the correlation with myelinated fiber pathology and T cell and macrophage invasions in the diseased nerves. Steady state levels of...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1022434209787
更新日期:1998-06-01 00:00:00