Abstract:
:Vascular endothelial growth factor (VEGF) is a promising biological marker and prognostic indicator in many neurological diseases. Although VEGF concentrations in plasma and cerebrospinal fluid (CSF) are increasingly reported, CSF-VEGF stability pre- and during-assay procedures is seldom evaluated. In the current study, we investigated VEGF variability and stability in CSF related to sample preparation, storage, and routine experimental procedures. Results showed that contaminant cell breakdown or aggregation can occur gradually before sample processing. However, after the removal of contaminant cell components, CSF-VEGF levels did not show significant changes in samples incubated at room temperature for 5 h, thawed/refrozen for 6 cycles. Samples preserved at -80 °C for up to 7 years continued to show measurable levels. Since some cellular components such as platelets contain a large amount of releasable VEGF, we conclude that CSF samples should be processed as soon as possible to carefully remove all cellular components and prevent possible consequent release of VEGF into CSF. After centrifugation to remove cellular contents, VEGF in CSF was relatively stable during routine experimental procedures and storage.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Yang J,Dombrowski SM,Deshpande A,Krajcir N,El-Khoury S,Krishnan C,Luciano MGdoi
10.1007/s11064-011-0517-zsubject
Has Abstractpub_date
2011-11-01 00:00:00pages
1947-54issue
11eissn
0364-3190issn
1573-6903journal_volume
36pub_type
杂志文章abstract::Donepezil is a selective inhibitor of acetylcholinesterase (AChE) clinically used for treating Alzheimer's disease. Cholinergic effects after short-term exposure of donepezil (up to 12 h) have been extensively studied in rats, but few have addressed the potential long-term effects. After 14 days administration (1x3 mg...
journal_title:Neurochemical research
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doi:10.1007/s11064-005-8828-6
更新日期:2005-12-01 00:00:00
abstract::The endogenous sialidase (N-acetylneuraminidase) activity of membranes prepared from goldfish retina and optic tectum displays characteristics similar to those reported for neural plasma membrane sialidases of other organisms. Endogenous membrane sialidase activity was found to be optimal at ph 4.0, and maximal releas...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965657
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journal_title:Neurochemical research
pub_type: 杂志文章
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journal_title:Neurochemical research
pub_type: 杂志文章,评审
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abstract::Chemotherapy drugs such as vincristine (VCR) can cause neuropathic pain, and there is still lack of ideal strategy to treat it. The current study was designed to investigate effect of matrine (MT) on VCR-induced neuropathic pain in animal model. VCR (75 μg/kg, i.p. for 10 consecutive days) was administered to induce p...
journal_title:Neurochemical research
pub_type: 杂志文章
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更新日期:2016-11-01 00:00:00
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journal_title:Neurochemical research
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-014-1484-y
更新日期:2015-03-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-006-9197-5
更新日期:2006-12-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-013-1083-3
更新日期:2013-09-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-011-0484-4
更新日期:2011-08-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/BF00966318
更新日期:1987-10-01 00:00:00
abstract::Metabotropic glutamate receptors (mGluR) play a role in synaptic transmission, neuronal modulation and plasticity but their action in epileptic activity is still controversial. On the other hand adenosine acts as a neuromodulator with endogenous anticonvulsive properties. Since cerebellum from epileptic patients has s...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-006-9275-8
更新日期:2007-07-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF01000040
更新日期:1989-04-01 00:00:00
abstract::We have utilized quantitative autoradiography with the GABA(A) receptor chloride channel blocker [35S]t-butylbicyclophosphorothionate ([35S]TBPS) in rodent brain sections to investigate if differential proton modulation of various GABA(A) receptor subtypes expressed in various brain regions are differentially sensitiv...
journal_title:Neurochemical research
pub_type: 杂志文章
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更新日期:2004-04-01 00:00:00
abstract::Previous studies have demonstrated that the c-Jun N-terminal kinase (JNK) pathway plays an important role in inducing neuronal apoptosis following cerebral ischemic injury. JNK signaling pathway in activated during cerebral ischemic injury. It participates in ischemia-induced neuronal apoptosis. However, whether JNK s...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-015-1753-4
更新日期:2016-04-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00975043
更新日期:1993-12-01 00:00:00
abstract::Neuronal perikarya were isolated, using bulk preparative procedures, from bovine brains. Synaptosomes, neuronal perikarya, and brain homogenates had similar ganglioside patterns, with the synaptosomes containing at least four times more total ganglioside per mg protein than the neuronal perikarya and twice that of the...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00966108
更新日期:1976-04-01 00:00:00
abstract::The decline in neuronal function during aging may result from increases in extracellular glutamate (Glu), Glu-induced neurotoxicity, and altered mitochondrial metabolism. To study metabolic responses to persistently high levels of Glu at synapses during aging, we used transgenic (Tg) mice that over-express the enzyme ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-014-1239-9
更新日期:2014-01-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-014-1236-z
更新日期:2014-02-01 00:00:00
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journal_title:Neurochemical research
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journal_title:Neurochemical research
pub_type: 杂志文章
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journal_title:Neurochemical research
pub_type: 杂志文章
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更新日期:2020-11-01 00:00:00
abstract::In vitro aggregation and fibrillization of synthetic amyloid beta-protein Abeta 1-40 was assessed in the conditioned media from rhabdomyosarcoma (CRL 1598, HTB 82, HTB 153, CCL 136), adenocarcinoma (CCL 218), neuroblastoma (SY5Y), and COS cells cultured in the absence and presence of 10% heat-inactivated fetal bovine ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1027379926976
更新日期:1997-02-01 00:00:00
abstract::Cholinergic muscarinic receptors, when stimulated by arecoline, can activate cytosolic phospholipase A(2) (cPLA(2)) to release arachidonic acid (AA) from membrane phospholipid. This signal can be imaged in the brain in vivo using quantitative autoradiography following the intravenous injection of radiolabeled AA, as a...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-007-9372-3
更新日期:2007-11-01 00:00:00
abstract::Traumatic brain injury (TBI) initiates a series of neurochemical and signaling changes that could eventually lead to neuronal apoptosis. Recent studies indicated that mature neurons cell cycle re-enter played a crucial role in neuronal apoptosis. In this study, we identified that the chaperonin containing TCP-1, subun...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-015-1683-1
更新日期:2015-09-01 00:00:00
abstract::Oxidative stress-induced neuronal cell death requires opening of the mitochondrial permeability transition pore. P53 mitochondrial translocation and association with Cyclophilin D (Cyp-D) is required for the pore opening. Here we tested this signaling axis in oxygen glucose deprivation (OGD)/re-oxygenation-induced in ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-013-0968-5
更新日期:2013-04-01 00:00:00
abstract::Detergents Triton X-100, sodium deoxycholate, and octyl-beta-D-glucopyranoside, and proteinase papain proved to be excellent agents solubilizing the gamma-glutamyltransferase (gamma-GT) from human brain cortex microvessels. Ficin also solubilized gamma-GT but to a lesser extent than papain. The relative molecular mass...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964524
更新日期:1984-07-01 00:00:00
abstract::It is thought that changes in gene expression in the brain mediate chronic ethanol-induced complex behaviors such as tolerance, dependence, and sensitization, and also relate to ethanol-induced brain toxicity. Using high-density filter-based cDNA microarrays (GeneFilters), we analyzed the expression of over 5000 genes...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1020937728506
更新日期:2002-10-01 00:00:00
abstract::The pathophysiological mechanism(s) underlying Alzheimer's disease (AD) still remain unclear, and no disease-modifying or prophylactic therapies are currently available. Unraveling the fundamental neuropathogenesis of AD is an important challenge. Several studies on AD have suggested lesions in a number of CNS areas i...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
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更新日期:2012-04-01 00:00:00
abstract::This review presents data on metabotropic uridine nucleotide receptor subtypes (P2UR) activated by UTP, sometimes also by UDP and/or ATP. Some chemical details of receptor subtypes and ligand interactions are described. Ligand-activated P2UR subtypes may couple to different second messengers, yet little is known about...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1023/a:1022487128766
更新日期:1997-08-01 00:00:00