Abstract:
:The decline in neuronal function during aging may result from increases in extracellular glutamate (Glu), Glu-induced neurotoxicity, and altered mitochondrial metabolism. To study metabolic responses to persistently high levels of Glu at synapses during aging, we used transgenic (Tg) mice that over-express the enzyme Glu dehydrogenase (GDH) in brain neurons and release excess Glu in synapses. Mitochondrial GDH is important in amino acid and carbohydrate metabolism and in anaplerotic reactions. We monitored changes in nineteen neurochemicals in the hippocampus and striatum of adult, middle aged, and aged Tg and wild type (wt) mice, in vivo, using proton ((1)H) magnetic resonance spectroscopy. Significant differences between adult Tg and wt were higher Glu, N-acetyl aspartate (NAA), and NAA + NAA-Glu (NAAG) levels, and lower lactate in the Tg hippocampus and striatum than those of wt. During aging, consistent changes in Tg and wt hippocampus and striatum included increases in myo-inositol and NAAG. The levels of glutamine (Gln), a key neurochemical in the Gln-Glu cycle between neurons and astroglia, increased during aging in both the striatum and hippocampus of Tg mice, but only in the striatum of the wt mice. Age-related increases of Glu were observed only in the striatum of the Tg mice.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Choi IY,Lee P,Wang WT,Hui D,Wang X,Brooks WM,Michaelis EKdoi
10.1007/s11064-014-1239-9subject
Has Abstractpub_date
2014-01-01 00:00:00pages
446-55issue
3eissn
0364-3190issn
1573-6903journal_volume
39pub_type
杂志文章abstract::The release of labeled norepinephrine from preloaded synaptosomes requires the presence of potassium and calcium. ATP-dependent binding of calcium to synaptic plasma membranes (SPM) may provide a means of maintaining the cation in a readily available pool for the triggering of transmitter release. A high Ca-binding ca...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965106
更新日期:1983-04-01 00:00:00
abstract::Acetylcholinesterase (AChE) inhibition has been described as the main mechanism of organophosphate (OP)-evoked toxicity. OPs represent a human health threat, because chronic exposure to low doses can damage the developing brain, and acute exposure can produce long-lasting damage to adult brains, despite post-exposure ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-015-1548-7
更新日期:2015-10-01 00:00:00
abstract::The synthetic Liver X receptor (LXR) activator T0901317 has been reported to exert neuroprotective effect in Alzheimer's disease, but the relationship between LXR activation and beta-site amyloid precursor protein cleaving enzyme 1 (BACE-1) remains uncertain. This study investigated the effect of T0901317 on membrane ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-011-0513-3
更新日期:2011-10-01 00:00:00
abstract::A role for protein phosphorylation in the process of neurite outgrowth has been inferred from many studies of the effects of protein kinase inhibitors and activators on cultured neurotumor cells and primary neuronal cells from developing brain or ganglia. Here we re-examine this issue, using a culture system derived f...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1021916509858
更新日期:1997-10-01 00:00:00
abstract::It has been established that oligodendrocytes, the myelin forming cells, participate in iron homeostasis through the synthesis and secretion of transferrin. Here we investigated whether a correlation exists between myelination, the commonly studied function of oligodendrocytes, and that of transferrin synthesis and se...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-004-1826-2
更新日期:1999-02-01 00:00:00
abstract::Charge microheterogeneity of myelin basic protein is known to affect its conformation and function. Here, the citrullinated myelin basic protein charge isomer, component-8, was shown to be more susceptible to stromelysin-1 cleavage than myelin basic protein component-1. Since levels of component-8 are increased in mul...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-006-9116-9
更新日期:2006-08-01 00:00:00
abstract::Ochratoxin A (OA) is a mycotoxin produced by several storage fungi, such as Aspergillus ochraceus and several Penicillium species. OA (3 mg/kg) was given intraperitoneally to pregnant mice on day 11 of gestation (day 1 = day of insemination), and neurochemical changes in brains of their offspring were examined at feta...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00971631
更新日期:1988-12-01 00:00:00
abstract::Radioactive 125I seeds-based radiotherapy has achieved great success in treatment of human cancers. However, radioresistance and severe side effects badly limited its clinic application. Recently, chemoradiotherapy as a superior strategy has been rapidly developed and widely used in clinic. However, the underlying mec...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-018-2547-2
更新日期:2018-07-01 00:00:00
abstract::Transgenic therapy for central neuralgia faces the problems of low expression and weak targeting and affects superficial but not deep neurons. In this study, we generated a lentivirus vector with human preproenkephalin gene (hPPE) expression driven by the transcriptional amplification strategy system (TAS) and establi...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-020-03068-1
更新日期:2020-09-01 00:00:00
abstract::The effects of neuroleptic treatments on dopamine transporters and on dopamine receptors was investigated in the forebrain of adult rats treated for 21 days with either haloperidol, clozapine or saline. The dopamine D1 receptors, labeled with [3H]SCH23390, increased in nucleus accumbens, latero-dorsal rostral neostria...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1022405621365
更新日期:1998-01-01 00:00:00
abstract::The uptake of radioactive ethanolamine has been studied in exclusively neuronal and glial cell cultures from dissociated cerebral hemispheres of chick embryos. Both cell types show saturable kinetics; neurons have an apparent Km of 6.7 microM, Vmax 41.4 pmol mg prot.-1 min-1 and glial cells a Km of 119.6 microM, Vmax ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965162
更新日期:1986-01-01 00:00:00
abstract::Attempts were made to solubilize acetylcholinesterase (AChE) from microsomal membranes isolated from rabbit white muscle. The preparative procedure included a step in which the microsomes were incubated in a solution containing high salt concentration (0.6 M KCl). About 15% of the total enzyme activity could be solubi...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00971007
更新日期:1987-07-01 00:00:00
abstract::Taurine is a semiessential amino acid that is not incorporated into proteins. In mammalian tissues, taurine is ubiquitous and is the most abundant free amino acid in the heart, retina, skeletal muscle, and leukocytes. Taurine reaches up to 50 mM concentration in leukocytes. Taurine has been shown to be tissue-protecti...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1023/b:nere.0000010440.37629.17
更新日期:2004-01-01 00:00:00
abstract::Epilepsy is one of the oldest neurological conditions known to humankind. It is known that oxidative stress and generation of reactive oxygen species are a cause and consequence of epileptic seizures. Although recent years have seen tremendous progress in the molecular biology and metabolism of selenium, we still know...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-009-0015-8
更新日期:2009-12-01 00:00:00
abstract::Alzheimer's disease (AD) is the most common type of neurodegenerative dementia that affects the elderly population. Nerve growth factor (NGF) contributes to the survival, regeneration and death of neurons during aging and in neurodegenerative diseases. Recently, research has shown that NGF is related to the pathology,...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-016-1829-9
更新日期:2016-06-01 00:00:00
abstract::The effect of 4-aminopyridine on [3H]acetylcholine release was studied in rat cerebral cortical synaptosomes in the presence of a several secretagogues that have different mechanisms of action. As found previously, 4-aminopyridine increased [3H]acetylcholine release in a concentration-dependent manner (5-10 mM); a hig...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00969632
更新日期:1989-02-01 00:00:00
abstract::Repeated electroconvulsive shock is an effective treatment for affective disorders. Striatum, hippocampus and brainstem are involved in affective disorders. Sodium-potassium/ATPase is of paramount importance for the proper functioning of the brain and its involvement in the affective disorders has been claimed for a l...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-006-9046-6
更新日期:2006-04-01 00:00:00
abstract::Endoplasmic reticulum (ER) and mitochondria are intracellular organelles and their interactions are directly involved in different processes such as Ca(2+) signaling in cell survival and death mechanisms. Bcl-2 is an anti-apoptotic protein intrinsically related to ER and mitochondria, modulating Ca(2+) content in thes...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-011-0600-5
更新日期:2012-02-01 00:00:00
abstract::Experimentalists would like to perform large-scale expression analysis on samples drawn from small cell populations and even single cells. Amplification of the source material is required to assay such small samples under all existing array technologies. In this report we consider the very different theoretical proper...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1020917223962
更新日期:2002-10-01 00:00:00
abstract::Alzheimer's disease (AD) is pathologically characterized by presence of senile plaques in the hippocampus, which are composed mainly of extracellular deposition of a polypeptide known as the beta amyloid, the Aβ. It has been demonstrated on numerous occasions that it was the deposition and aggregation of this Aβ pepti...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-012-0846-6
更新日期:2012-10-01 00:00:00
abstract::The synaptic glutamate level homeostasis is mainly maintained by the astrocytes membrane bound glutamate transporter type-1 (GLT-1/EAAT2). Alterations in its expression during development and aging and the underlying mechanisms are not well studied. Here, we report that NF-κB interaction was highest in both cerebral a...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-013-1200-3
更新日期:2014-01-01 00:00:00
abstract::There is increasing evidence that proteins normally involved in the cell cycle play a role in the regulation of neuronal apoptotic death following various insults. However, it is not clear if the same mechanisms regulate cell death of oligodendrocytes as well. In this study, we investigated the mechanism of ceramide-i...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/b:nere.0000021236.32785.37
更新日期:2004-05-01 00:00:00
abstract::Alzheimer's disease is a neurodegenerative disease characterized by the production of β-amyloid proteins and hyperphosphorylation of tau protein. Inflammation and apoptotic severity were highly correlated with earlier age at onset of Alzheimer's disease and were also associated with cognitive decline. This study aims ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-012-0866-2
更新日期:2012-12-01 00:00:00
abstract::Folic acid plays an important role in neuroplasticity and acts as a neuroprotective agent, as observed in experimental brain ischemia studies. The aim of this study was to investigate the effects of folic acid on locomotor activity, aversive memory and Na(+),K(+)-ATPase activity in the frontal cortex and striatum in a...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-012-0757-6
更新日期:2012-08-01 00:00:00
abstract::Rhesus macaque monkey brain microvessel endothelial cells (BMECs) were isolated and grown in culture in an effort to establish an appropriate primate in vitro model of the endothelial component of the blood-brain barrier. The presence of Factor VIII antigen, alkaline phosphatase, gamma-glutamyl transpeptidase, lactate...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00967320
更新日期:1994-04-01 00:00:00
abstract::Astrocytes have been considered to be transformed from radial glial cells that appear at early stage of development and play a scaffold-role for neuronal cell migration. Recent studies indicate that neuroepithelial cells in the spinal cord also give rise to astrocytes. However, the mode of astroglial generation and mi...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-008-9590-3
更新日期:2008-07-01 00:00:00
abstract::The aim of this study was to investigate potential therapeutic effects of IFN-γ primed human umbilical cord mesenchymal stem cell (IFN-γ-hUCMSCs) transplantation on experimental autoimmune encephalomyelitis (EAE) in mice. In this study, EAE mouse model was established by MOG35-55 immunization method. Outcomes of the E...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-020-03009-y
更新日期:2020-07-01 00:00:00
abstract::Autoantibodies to three eukaryotic 60S ribosomal phosphoproteins P0, P1 and P2 have been found in the sera of 10-20% of patients with systemic lupus erythematosus (SLE). These antibodies inhibit protein synthesis in vitro and when microinjected into cultured human fibroblasts. The three proteins share a common epitope...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00969925
更新日期:1990-04-01 00:00:00
abstract::Intracerebral folate injections produce convulsions and brain lesions, folic acid itself and tetrahydrofolate being more potent toxins than 5-methyltetrahydrofolate, the primary folate of mammalian extracellular fluids. Folates are known to excite neurons, by unknown mechanisms Folates stimulate GTP binding and GTPase...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00973648
更新日期:1990-07-01 00:00:00
abstract::Exposure to sevoflurane and other inhalational anesthetics can induce cognitive impairment in elderly patients. Studies have indicated that methylene blue (MB) has beneficial effects on multiple neurodegenerative diseases and the mechanism involves mitochondrial function preservation. However, whether MB can attenuate...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-020-02976-6
更新日期:2020-04-01 00:00:00