Abstract：
:The effect of 4-aminopyridine on [3H]acetylcholine release was studied in rat cerebral cortical synaptosomes in the presence of a several secretagogues that have different mechanisms of action. As found previously, 4-aminopyridine increased [3H]acetylcholine release in a concentration-dependent manner (5-10 mM); a high concentration (10 mM) also elevated [3H]choline efflux. However, the 35 mM K+ induced release of [3H]acetylcholine was attenuated by 4-aminopyridine at concentrations (less than 5 mM) that had no effect on transmitter release. At no concentration of 4-aminopyridine was the release of transmitter additive with 35 mM K+ induced release. Veratridine-induced release was neither attenuated nor additive with low concentrations of 4-aminopyridine, even when a sub-maximal concentration of the sodium ionophore was used (10 microM). In contrast, A23187-induced release was additive with that caused by 4-aminopyridine. These results suggest that: 1) 4-aminopyridine blocks potassium channels involved in regulating membrane potential in isolated cholinergic terminals; and 2) changes in the activity of these 4-aminopyridine sensitive K+ channels are not important in the nerve terminal's response to depolarization caused by sodium influx.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Meyer EM,Otero DHdoi
10.1007/BF00969632subject
Has Abstractpub_date
1989-02-01 00:00:00pages
157-60issue
2eissn
0364-3190issn
1573-6903journal_volume
14pub_type
杂志文章abstract：:In this review we examine successively: 1) the major effects of thyroid hormone deficiency seen during brain development with special emphasis on the changes in neuronal morphology and migration occurring postnatally in the cerebellum. 2) The effects of this hormone on microtubule assembly during neurite outgrowth and...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/BF00965840
更新日期：1991-09-01 00:00:00
abstract：:Pyridostigmine bromide (PB), an inhibitor of acetylcholinesterase, has been used as a prophylactic for nerve gas poisoning. N,N'-diethyl-m-toluamide (DEET) is the active ingredient in most insect repellents and is thought to interact synergistically with PB. Since PB can inhibit the binding of organophosphates to tubu...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1007527129743
更新日期：2000-01-01 00:00:00
abstract：:Adult rabbit brain contains the enzymatic machinery to convert deoxyuridine to deoxyuridine triphosphate (dUTP). Although dUTP as dUMP can be readily incorporated into DNA in place of thymidine monophosphate, we detected no (3H)dUMP in newly synthesized (3H)DNA in adult rabbit brain after the intraventricular injectio...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965201
更新日期：1983-08-01 00:00:00
abstract：:The central control of growth hormone (GH) secretion from the pituitary gland is ultimately achieved by the interaction between two hypothalamic neurohormones, somatostatin which inhibits and growth hormone-releasing hormone (GHRH) which stimulates GH release. The regulation of the somatostatin and GHRH release from t...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-005-9017-3
更新日期：2006-02-01 00:00:00
abstract：:Examination of the cytoskeleton has demonstrated the pivotal role of regulatory proteins governing cytoskeletal dynamics. Most work has focused on cell cycle and cell migration regarding cancer. However, these studies have yielded tremendous insight for development, particularly in the nervous system where all major c...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-006-9127-6
更新日期：2007-02-01 00:00:00
abstract：:Mutations in presenilin 1 (PS-1) are associated with most early-onset familiar Alzheimer's disease (AD). Previous studies have demonstrated that PS-1 mutations enhance the production of beta-amyloid (Aβ). In this study, we further examined the in vivo effects of PS-1 mutation on tau and synapse protein markers. The da...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-011-0575-2
更新日期：2012-01-01 00:00:00
abstract：:The effect of ageing and the relationships between the catalytic properties of enzymes linked to Krebs' cycle, electron transfer chain, glutamate and aminoacid metabolism of cerebral cortex, a functional area very sensitive to both age and ischemia, were studied on mitochondria of adult and aged rats, after complete i...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-009-0004-y
更新日期：2009-12-01 00:00:00
abstract：:VGF nerve growth factor inducible (VGF) is a neuropeptide precursor induced by brain-derived neurotrophic factor and nerve growth factor. VGF is increased in the prefrontal cortex and cerebrospinal fluid in schizophrenia patients. In our previous study, VGF-overexpressing mice exhibited schizophrenia-like behaviors an...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-018-2684-7
更新日期：2019-02-01 00:00:00
abstract：:The monoclonal antibody 14E immunocytochemically stains the nuclear membrane of oligodendrocytes but not myelin in tissue sections of adult normal human white matter. The nuclear membranes of Schwann cells in human peripheral nerve and cerebellar Bergmann glia were also visualized with this antibody. In actively demye...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00993270
更新日期：1992-09-01 00:00:00
abstract：:Oxidation of [2,3-14C]succinate in the intramitochondrial Krebs cycle was used as a probe to investigate the effect of ammonia on protein incorporation and Krebs cycle oxidation of succinate carbons in isolated rat hepatocytes. At low concentrations of ammonium chloride (0.1 to 0.5 mM) a slight increase in 14CO2 forma...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965690
更新日期：1991-07-01 00:00:00
abstract：:Cold restraint stress (CRS) model exerts similar effect as physiological stress because it combines emotional stress (escape reaction) and physical stress (muscle work). It is well established that various responses to stress are regulated by sympathoadrenal system, brain monoaminergic systems and oxidative processes....
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-012-0867-1
更新日期：2012-12-01 00:00:00
abstract：:Bovine brain synaptic membranes which were frozen and then extensively washed showed low affinity [3H]muscimol binding. These membranes contained GABA and calmodulin, apparently tightly bound within the membrane fraction. Membranes which were additionally treated with the detergent Triton X-100 showed high affinity [3...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00970756
更新日期：1988-09-01 00:00:00
abstract：:The stability of somatostatin (SS) in brain tissue was studied in human material obtained post-mortem and in the rat. In both human and rat brain, loss of SS was found to occur in tissue frozen to -70 degrees C. In the rat, this loss varied from 26 to 70 percent depending on the type of tissue processing used. These d...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00973289
更新日期：1988-06-01 00:00:00
abstract：:In order to investigate the role of two free radical detoxificant enzymes in patients with aging brain disorders, superoxide dismutase (SOD) and catalase (CAT) activities have been measured in blood from male and female human patients of different ages with several types of aging brain disorders. When compared with ac...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF02532336
更新日期：1996-08-01 00:00:00
abstract：:N-Acetylaspartic and N-acetylaspartylglutamic acid concentrations in human ventricular, subarachnoid and lumbar cerebrospinal fluid were measured by combined gas chromatography-mass spectrometry using selected ion monitoring with deuterated internal standards. N-Acetylaspartate concentrations were in the range 55, 9, ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1020973023059
更新日期：1999-10-01 00:00:00
abstract：:Schwann cell biosynthesis of glycolipids was studied by in vitro incorporation of [3H]galactose into neonatal rat sciatic nerves before and after endoneurial explant culture and in culture of purified Schwann cells. In neonatal nerves prior to culture, [3H]galactose was actively incorporated into galactocerebrosides (...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00968672
更新日期：1990-03-01 00:00:00
abstract：:The aim of this study was to explore effects of hypoxia, glucose deprivation (HGD) and recovery on expression and activities of equilibrative nucleoside transporters (rENT) and concentrative nucleoside transporters (rCNT) in rat astrocytes in primary culture. Amounts of cellular ATP in the control group (CG, 5% CO(2) ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-010-0203-6
更新日期：2010-09-01 00:00:00
abstract：:Epilepsy is a disorder of the brain characterized by an enduring predisposition to generate epileptic seizures. The glycolytic inhibitor 2-deoxy-D-glucose (2-DG) has been reported to exert antiepileptic effects by upregulating KATP subunits (kir6.1 and kir6.2). We evaluated whether 2-DG exhibits anti-seizure effect by...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-019-02734-3
更新日期：2019-04-01 00:00:00
abstract：:Epilepsy is one of the oldest neurological conditions known to humankind. It is known that oxidative stress and generation of reactive oxygen species are a cause and consequence of epileptic seizures. Although recent years have seen tremendous progress in the molecular biology and metabolism of selenium, we still know...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-009-0015-8
更新日期：2009-12-01 00:00:00
abstract：:Social isolation during early development is one of the most potent stressors that can cause alterations in the processes of brain maturation, leading to behavioral and neurochemical changes that may persist to adulthood. Exposure to palatable diets during development can also affect neural circuits with long-term con...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-013-1083-3
更新日期：2013-09-01 00:00:00
abstract：:Protein kinase C (PKC) plays a key role in cellular events including proliferation, survival and differentiation. Our previous study showed the effect of phorbol 12-myristate 13-acetate (PMA), a PKC activator, inducing a decrease in retinal cells proliferation. This effect was mediated by muscarinic type 1 receptors (...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-008-9847-x
更新日期：2009-05-01 00:00:00
abstract：:Several epidemiological studies have shown that consumption of large quantities of vegetables especially cruciferous vegetables (Broccoli and Brussels sprouts) can protect against chronic diseases. Sulforaphane, an isothiocynate found in cruciferous vegetables has been demonstrated to have neuroprotective effects in s...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-016-1965-2
更新日期：2016-10-01 00:00:00
abstract：:gamma-L-Glutamylglutamate (LGG), an endogenous constituent of the brain, reduced the glutamate-evoked increase in intracellular Ca2+ in cultured cerebellar granule cells. The extent and properties of this inhibition were different at different Mg2+ concentrations. The intracellular Ca2+ response to NMDA was slightly e...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00970596
更新日期：1995-12-01 00:00:00
abstract：:Exploring the neurochemical continuum between frontotemporal dementia (FTD) and amyotrophic lateral sclerosis (ALS) with respect to monoamines and kynurenines in cerebrospinal fluid (CSF) and serum, may be useful to identify possible new research/therapeutic targets. Hence, we analysed monoamines and kynurenines in CS...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-020-03002-5
更新日期：2020-05-01 00:00:00
abstract：:The folate cycle, which has a close correlation with nucleic acid synthesis as well as with sulfur amino acid metabolism, may have some bearing on the pathogenesis of amyotrophic lateral sclerosis. The folate cycle hypothesis offers a unified explanation of the reduction of RNA and the elevation of taurine in nervous ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00963985
更新日期：1984-03-01 00:00:00
abstract：:Excessive hyperbilirubinemia can cause irreversible neurological damage in the neonatal period. However, the complete understanding of the pathogenesis of unconjugated bilirubin (UCB) encephalopathy remains a matter of debate. This study investigates whether UCB inhibits the endocytosis of cationized ferritin (CF) by ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1011608017870
更新日期：2001-07-01 00:00:00
abstract：:Amphiphile dependency, solubility in aqueous solutions, and sensitivity to proteolysis of acetylcholinesterase (AChE) and nonspecific cholinesterase (nsChE) in the rat superior cervical ganglion were studied and compared to properties of soluble plasma cholinesterases. Ganglion AChE shows strong amphiphile dependency:...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964537
更新日期：1985-06-01 00:00:00
abstract：:We examined the effects of small unilamellar vesicles composed of dipalmitoylphosphatidylcholine on rat cerebral cortical [3H]acetylcholine release. Synaptosomes from this region were loaded with the labeled transmitter, and then incubated with the lipid (0-6 mg/ml) for specified intervals before adding various secret...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00970530
更新日期：1987-08-01 00:00:00
abstract：:The interactions of S-nitrosoglutathione (GSNO) with the ionotropic glutamate receptors were studied on synaptic membranes isolated from the pig cerebral cortex. GSNO displaced the binding of [3H]glutamate, 3-[(R)-2-carboxypiperazin-4-yl] [3H]propyl-1-phosphonate ([3H]CPP), a competitive N-methyl-D-aspartate (NMDA) an...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1007626230278
更新日期：2000-08-01 00:00:00
abstract：:Glycogen is the main storage form of glucose in the brain. In contrast with previous beliefs, brain glycogen has recently been shown to play important roles in several brain functions. A fraction of metabolized glucose molecules are being shunted through glycogen before reentering the glycolytic pathway, a phenomenon ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-017-2267-z
更新日期：2017-09-01 00:00:00
