Abstract：
:Oxidation of [2,3-14C]succinate in the intramitochondrial Krebs cycle was used as a probe to investigate the effect of ammonia on protein incorporation and Krebs cycle oxidation of succinate carbons in isolated rat hepatocytes. At low concentrations of ammonium chloride (0.1 to 0.5 mM) a slight increase in 14CO2 formation from [2,3-14C]succinate was observed, however, the stimulatory effect of insulin was significantly reduced. Insulin failed to cause any stimulation of succinate carbons incorporation into hepatocyte protein in the presence of ammonium chloride. Addition of ammonium chloride also depressed the movement of tracer carbons into the gluconeogenesis pathway. The activity of the amphibolic amino acid pool was significantly enhanced by ammonia. The data presented in this paper lend strong support to the Krebs-cycle depletion theory of hepatic coma. They also suggest that reduced mitochondrial Krebs cycle activity caused by increased amphibolic depletion of substrates results in loss of insulin sensitivity in ammonia toxicity.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Bessman SP,Wang W,Mohan Cdoi
10.1007/BF00965690subject
Has Abstractpub_date
1991-07-01 00:00:00pages
805-11issue
7eissn
0364-3190issn
1573-6903journal_volume
16pub_type
杂志文章abstract：:When excised goldfish spinal cord is kept in physiological saline at room temperature, the myelin multilayers swell. As studied by X-ray diffraction, the original repeating distance of 150 A (AS myelin) swells to 177 A (AL myelin); no intermediate distances are seen. At least 70% of the myelin can undergo this gradual...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965941
更新日期：1986-08-01 00:00:00
abstract：:Radioactive azide derivatives of tetrodotoxin (TTX) were synthesized using 2-nitro-4-azidephenyl-[3H]beta alanine for the purpose of photolabeling of the Na channel. Three azide derivatives, N1, N2 and N3, were separated by ion exchange chromatography on Bio-Rex 70 resin and reversed phase high performance liquid chro...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965886
更新日期：1985-08-01 00:00:00
abstract：:DNase I sensitivity experiments were performed utilizing DNA probes to genes which are either transcribed in rat cortical neurons (the 68 kDa neurofilament gene and the neuron-specific enolase gene) or are transcriptionally silent (albumin). Results suggest that unlike liver, in which a hierarchy in chromatin conforma...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00969627
更新日期：1989-02-01 00:00:00
abstract：:One pathway by which tryptophan is metabolized in the brain as well as in the periphery is through cleavage of the indole ring to formylkynurenine and then kynurenine. Indoleamine-2,3-dioxygenase, the enzyme that catalyzes this cleavage, and kynurenine are distributed all across the different anatomic regions of brain...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/BF00964611
更新日期：1980-03-01 00:00:00
abstract：:A case-control study was undertaken to investigate the status of platelet monoamine oxidase-B (MAO-B) activity in Indian cases of idiopathic Parkinson's disease. A significant increase in the activity of platelet MAO-B was observed in Parkinson's cases (n = 26) as compared to controls (n = 26). No significant change i...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-009-9929-4
更新日期：2009-08-01 00:00:00
abstract：:Mitochondrial division inhibitor 1 (Mdivi-1) is the most effective pharmacological inhibitor of mitochondrial fission. Spinal cord injury (SCI) is a common and serious trauma, which lacks efficient treatment. This study aimed to detect the role of Mdivi-1 in neuronal injury and its underlying mechanism after acute SCI...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-015-1604-3
更新日期：2015-07-01 00:00:00
abstract：:Several studies showed that the up-regulation of glial glutamate transporter-1 (GLT-1) participates in the acquisition of brain ischemic tolerance induced by cerebral ischemic preconditioning or ceftriaxone pretreatment in rats. To explore whether GLT-1 plays a role in the acquisition of brain ischemic tolerance induc...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-011-0639-3
更新日期：2012-03-01 00:00:00
abstract：:We evaluated oxidative stress associated with a model of experimental epilepsy. Male Wistar rats were injected i.p. with 150 mg/kg convulsant 3-mercaptopropionic acid and decapitated in two stages: during seizures or in the post-seizure period. Spontaneous chemiluminescence, levels of thiobarbituric acid reactive subs...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF03177490
更新日期：1998-12-01 00:00:00
abstract：:"Kindling" is a phenomenon of epileptogenesis, which has been widely used as an experimental model of temporal lobe epilepsy. At the present work we investigated the contribution of NMDA receptors in the Pentylenetetrazol-induced "kindling" model in the mouse brain, by using quantitative autoradiography and the radioa...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1021143813935
更新日期：1999-12-01 00:00:00
abstract：:We have investigated gene expression changes produced by acute and chronic daily treatment with a prototypical antidepressant, imipramine, using DNA microarrays. The analysis of similarities in gene expression patterns among functionally related genes revealed four expression profile cluster areas that showed a highly...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/b:nere.0000023610.89631.d6
更新日期：2004-06-01 00:00:00
abstract：:The specific molecular events that underlie the age-related loss of cognitive function are poorly understood. Although not experimentally substantiated, age-dependent neuronal loss has long been considered central to age-related cognitive decline. More recently, age-related changes in brain white matter have taken pre...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-007-9341-x
更新日期：2007-12-01 00:00:00
abstract：:It has been proposed that taurine may function as an oxidant in a dose-dependent manner in vivo and in vitro. The present study was carried out to investigate the relationship between taurine concentration and 3-nitrotyrosine level, a stable marker of peroxynitrite action, in hepatocytes of guinea pig in endotoxemia b...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-007-9395-9
更新日期：2007-11-01 00:00:00
abstract：:The conversion of phosphoethanolamine to phosphocholine requires 3 separate N-methyltransferases. We had previously purified the enzyme catalyzing the last methylation, phosphodimethylethanolamine N-methyltransferase. We have successfully purified the enzyme catalyzing the initial methylation of phosphoethanolamine. A...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00995388
更新日期：1995-10-01 00:00:00
abstract：:Cationic lipid formulations consisting of 3beta [N-(N', N'-dimethylaminoethane)-carbamoyl] cholesterol (DC-Chol) and the helper lipid dioleoylphosphatidylethanolamine (DOPE) (1.5: 1 molar ratio) were prepared by solvent evaporation and sized by high pressure extrusion. Liposomes made of 1:1 molar ratio 1 ,2-dioleoyl-3...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1021012727796
更新日期：1999-05-01 00:00:00
abstract：: ...
journal_title：Neurochemical research
pub_type： 传,社论,历史文章
doi：10.1007/s11064-018-02702-3
更新日期：2019-01-01 00:00:00
abstract：:The secretion of fluid and electrolytes by salivary gland acinar cells requires the coordinated regulation of multiple ion channel and transporter proteins, signaling components, and water transport. Importantly, neurotransmitter stimulated increase in the cytosolic free [Ca(2+)] ([Ca(2+)](i)) is critical for the regu...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-011-0465-7
更新日期：2011-07-01 00:00:00
abstract：:Alpha adrenergic receptor subtypes in rat hippocampal membranes were studied, using [3H] clonidine as the radioactive ligand. On the basis of competitive binding studies, using the selective antagonist-prazosin, WB-4101, and yohimbine, [3H] clonidine appeared to bind to a population of presynaptic sites that are pharm...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965058
更新日期：1982-02-01 00:00:00
abstract：:Glutamate dehydrogenase (GDH) is a homohexameric enzyme that catalyzes the reversible oxidative deamination of L-glutamate to 2-oxoglutarate. Only in the animal kingdom is this enzyme heavily allosterically regulated by a wide array of metabolites. The major activators are ADP and leucine and inhibitors include GTP, p...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-013-1173-2
更新日期：2014-01-01 00:00:00
abstract：:A variety of naturally occurring amino acids, their isomers, and synthetic analogs were tested for their ability to inhibit uptake of [3H]glutamate into presynaptic vesicles from bovine cerebral cortex. Strongest inhibition (Ki < 1mM) was observed for trans-1-aminocyclopentane-1,3-dicarboxylic acid (t-ACPD) and erythr...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966925
更新日期：1993-01-01 00:00:00
abstract：:In this review we examine successively: 1) the major effects of thyroid hormone deficiency seen during brain development with special emphasis on the changes in neuronal morphology and migration occurring postnatally in the cerebellum. 2) The effects of this hormone on microtubule assembly during neurite outgrowth and...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/BF00965840
更新日期：1991-09-01 00:00:00
abstract：:Cell surface β-Amyloid precursor protein (APP) is known to have a functional role in iron homeostasis through stabilising the iron export protein ferroportin (FPN). Mechanistic evidence of this role has previously only been provided through transcriptional or translational depletion of total APP levels. However, numer...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-019-02747-y
更新日期：2019-06-01 00:00:00
abstract：:Prevention and amelioration of Mibyou (sub-healthy condition) is the critical target for disease prevention including age-related diseases and cancer although the Mibyou condition is not yet pathologically defined. Since the oxidative stress is an underlying basic etiology associated with many diseases and aging, the ...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-008-9872-9
更新日期：2009-04-01 00:00:00
abstract：:Bovine brain synaptic membranes which were frozen and then extensively washed showed low affinity [3H]muscimol binding. These membranes contained GABA and calmodulin, apparently tightly bound within the membrane fraction. Membranes which were additionally treated with the detergent Triton X-100 showed high affinity [3...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00970756
更新日期：1988-09-01 00:00:00
abstract：:Diisopropyl phosphorofluoridate (DFP) produces organophosphorus ester-induced delayed neurotoxicity (OPIDN) in sensitive species. We have investigated the in vivo and in vitro effects of DFP on hen brain tubulin polymerization. Hens were treated with a single dose of DFP (1.7 mg/kg, sc.), and were sacrificed after 18-...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00967321
更新日期：1994-04-01 00:00:00
abstract：:A synthetic peptide corresponding to the first 28 amino acids of the Alzheimer disease amyloid beta/A4 peptide (3.2 kDa) aggregated to a high molecular weight (15 kDa) on SDS/urea polyacrylamide gels. Proteinase K, V8 protease, trypsin, and endopeptidase Lys-C readily degraded the aggregate. By contrast, when digested...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00974579
更新日期：1992-04-01 00:00:00
abstract：:We review some of the older literature concerning metabolic turnover of cholesterol in the nervous system. The overall picture is that incorporation of radioactive precursors into brain cholesterol is roughly proportional to the rate of myelination and that, once incorporated, radioactive cholesterol is relatively sta...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/BF02527711
更新日期：1996-04-01 00:00:00
abstract：:Hyperprolinemia type II (HPII) is an autosomal recessive disorder caused by the severe deficiency of enzyme delta1-pyrroline-5-carboxylic acid dehydrogenase leading to tissue accumulation of proline. Chronic administration of Pro led to significant reduction of cytosolic ALT activity of olfactory lobes (50.57%), cereb...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-004-7026-2
更新日期：2004-12-01 00:00:00
abstract：:Beta-amyloid (Abeta) peptides may cause malfunction and death of neurons in Alzheimer's disease. We investigated the effect of Abeta on key transporters of amino acid neurotransmission in cells cultured from rat cerebral cortex. The cultures were treated with Abeta(25-35) at 3 and 10 microM for 12 and 24 h followed by...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-007-9527-2
更新日期：2008-02-01 00:00:00
abstract：:Microglia can exacerbate central nervous system disorders, including stroke and chronic progressive neurodegenerative diseases such as Alzheimer disease. Mounting evidence points to ion channels expressed by microglia as contributing to these neuropathologies. The Chloride Intracellular Channel (CLIC) family represent...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-013-1084-2
更新日期：2013-09-01 00:00:00
abstract：:Excessive hyperbilirubinemia can cause irreversible neurological damage in the neonatal period. However, the complete understanding of the pathogenesis of unconjugated bilirubin (UCB) encephalopathy remains a matter of debate. This study investigates whether UCB inhibits the endocytosis of cationized ferritin (CF) by ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1011608017870
更新日期：2001-07-01 00:00:00
