Abstract：
:Beta-amyloid (Abeta) peptides may cause malfunction and death of neurons in Alzheimer's disease. We investigated the effect of Abeta on key transporters of amino acid neurotransmission in cells cultured from rat cerebral cortex. The cultures were treated with Abeta(25-35) at 3 and 10 microM for 12 and 24 h followed by quantitative analysis of immunofluorescence intensity. In mixed neuronal-glial cell cultures (from P1 rats), Abeta reduced the concentration of system A glutamine transporter 1 (SAT1), by up to 50% expressed relative to the neuronal marker microtubule-associated protein 2 (MAP2) in the same cell. No significant effects were detected on vesicular glutamate transporters VGLUT1 or VGLUT2 in neurons, or on glial system N glutamine transporter 1 (SN1). In neuronal cell cultures (from E18 rats), Abeta(25-35) did not reduce SAT1 immunoreactivity, suggesting that the observed effect depends on the presence of astroglia. The results indicate that Abeta may impair neuronal function and transmitter synthesis, and perhaps reduce excitotoxicity, through a reduction in neuronal glutamine uptake.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Buntup D,Skare O,Solbu TT,Chaudhry FA,Storm-Mathisen J,Thangnipon Wdoi
10.1007/s11064-007-9527-2subject
Has Abstractpub_date
2008-02-01 00:00:00pages
248-56issue
2eissn
0364-3190issn
1573-6903journal_volume
33pub_type
杂志文章abstract：:Adult rabbit brain contains the enzymatic machinery to convert deoxyuridine to deoxyuridine triphosphate (dUTP). Although dUTP as dUMP can be readily incorporated into DNA in place of thymidine monophosphate, we detected no (3H)dUMP in newly synthesized (3H)DNA in adult rabbit brain after the intraventricular injectio...
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