Abstract：
:The 7S NGF complex from the male mouse submaxillary gland consists of the alpha, gamma, and beta subunits in the ratio alpha 2 gamma 2 beta. The beta (NGF) subunit contains all the known biolocial activity of 7S NGF. The alpha and gamma subunits are both members of glandular kallikrein gene family, yet only gamma subunit has protease activity. The gamma subunit plays a role in the processing of preproNGF to its mature form, while the role of the alpha subunit is not yet understood. Despite the fact that 7S NGF has been extensively characterized, no other NGF complex has been characterized, nor have the alpha or gamma subunits been observed in tissues which express NGF. We have therefore purified and characterized the NGF complex from the submaxillary glands of the rat Mastomys natalensis in order to more fully understand the roles of the alpha and gamma subunits. The NGF complex from M. natalensis contains subunits similar to those found in mouse 7S NGF. Although similar, there are significant differences between mouse and M. natalensis NGF complexes, especially in the degree of post-translational modification of the gamma and NGF subunits, the expression of esterase activity and the ease with which the complexes dissociate. Evidence is presented that suggests that the NGF complex from M. natalensis may consist of subunits in the ratio alpha 2 gamma beta. The amino acid sequence of the M. natalensis NGF suggests some, but not all, ways in which these differences arise.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Burcham TS,Sim I,Bolin LM,Shooter EMdoi
10.1007/BF00965545subject
Has Abstractpub_date
1991-06-01 00:00:00pages
603-12issue
6eissn
0364-3190issn
1573-6903journal_volume
16pub_type
杂志文章abstract：:This article puts forward the hypothesis that the Low Density Lipid Receptor (LDLR) is one of the molecules that is involved in the clearance of amyloid proteins in the brain and that it may play a role in Alzheimer's Disease (AD) via its up-regulation by statins. The hypothesis is built on the following observations:...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-006-9084-0
更新日期：2006-06-01 00:00:00
abstract：:A double ligand enzyme-linked immunosorbent assay (ELISA) has been developed to detect antibodies against brain tissue antigens in the sera of patients with neurological diseases. The sera were tested on human white matter homogenate. The technique consists of successive incubations with the human serum to be tested, ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964651
更新日期：1985-04-01 00:00:00
abstract：:Evidence was accumulated indicating that cyclic nucleotides are involved in regulation of growth, differentiation and function of lymphoid cells. It was previously shown that the N-fragment (1-4) of thymosin beta 4 (Ac-Ser-Asp-Lys-Pro-OH) inhibits in vivo the entry of cell populations into S-phase. In the course of th...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00995153
更新日期：1995-01-01 00:00:00
abstract：:The NG 108-15 (neuroblastoma X glioma hybrid) cell line was used as an in vitro neuronal model to evaluate potential antagonists of the Na+-selective carboxylic ionophore monensin. Changes in membrane electrical characteristics induced by monensin with and without the simultaneous administration of antagonists were me...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1007548307554
更新日期：2000-07-01 00:00:00
abstract：:The patterns of cholesterol content in chick brain and liver were studied during embryonic development and compared with the variations in the specific activities of mevalonate-activating enzymes during the same period. Total cholesterol content in both embryonic chick brain and liver increased during incubation. The ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964728
更新日期：1983-06-01 00:00:00
abstract：:6-Hydroxydopamine (6-OHDA) induces the production of reactive oxygen species (ROS) that are associated with various neurodegenerative diseases such as Parkinson's disease. 3,3',4',7-Tetrahydroxyflavone (fisetin), a plant flavonoid has a variety of physiological effects such as antioxidant activity. In this study, we i...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-017-2445-z
更新日期：2018-02-01 00:00:00
abstract：:We found that chronic lithium diet affects the sensitivity of neuroleptic receptors and the content of amino acids in the brain, and that the changes in adult animals differ from those in young rats. Pregnant rats were kept on lithium diet (pellets with 0.21% Li2CO3 and 0.21% NaCl) during the gestation period and the ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964703
更新日期：1983-05-01 00:00:00
abstract：:The aim of this study was to investigate potential therapeutic effects of IFN-γ primed human umbilical cord mesenchymal stem cell (IFN-γ-hUCMSCs) transplantation on experimental autoimmune encephalomyelitis (EAE) in mice. In this study, EAE mouse model was established by MOG35-55 immunization method. Outcomes of the E...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-020-03009-y
更新日期：2020-07-01 00:00:00
abstract：:We have previously shown that 5-methyltetrahydrofolate influences neuro-secretion. The present study more precisely characterises the processes involved and considers one probable site of action. Focusing on the tyrosine-noradrenalin axis in cerebellum we showed 5-methyltetrahydrofolate causes a significant reduction ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF01705542
更新日期：1995-06-01 00:00:00
abstract：:Discrete populations of anti-S49 antibodies were found in the antisera of Lewis rats recovered from S49-induced experimental allergic encephalomyelitis (EAE). A potent inducer of EAE in Lewis rats, S49 is a synthetic peptide representing residues 69-84 of bovine myelin basic protein but with deletions at Gly-77 and Hi...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00988601
更新日期：1985-12-01 00:00:00
abstract：: ...
journal_title：Neurochemical research
pub_type： 传,社论,历史文章
doi：10.1007/s11064-018-02702-3
更新日期：2019-01-01 00:00:00
abstract：:Blue light impinging on the many mitochondria associated with retinal ganglion cells (RGCs) in situ has the potential of eliciting necroptosis through an action on RIP1/RIP3 to stimulate RGC death in diseases like glaucoma and diabetic retinopathy. Cells in culture die when exposed to blue light. The death process is ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-016-1946-5
更新日期：2016-09-01 00:00:00
abstract：:Antiretroviral protease inhibitors are a class of important drugs that are used for the treatment of human immunodeficiency virus infections. Among those compounds, ritonavir is applied frequently in combination with other antiretroviral protease inhibitors, as it has been reported to boost their therapeutic efficienc...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-013-0971-x
更新日期：2013-04-01 00:00:00
abstract：:The wobbler mouse suffers an autosomal recessive mutation producing severe neurodegeneration and astrogliosis in spinal cord. It has been considered a model for amyotrophic lateral sclerosis. We have studied in these animals the expression of two proteins, the growth-associated protein (GAP-43) and the NADPH-diaphoras...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1020918310281
更新日期：1999-01-01 00:00:00
abstract：:CNS trauma has been associated with an increase in free radical production, but the cellular sources of this increase or the mechanism involved in the production of free radicals are not known. We, therefore, investigated the effects of trauma on free radical production in cultured neurons, astrocytes and BV-2 microgl...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1014875210852
更新日期：2002-02-01 00:00:00
abstract：:Microdissection of selected regions of central nervous system (CNS) has provided the basis of modern chemoarchitectonics. Laser microdissection is a modern variant of the "Palkovits punch" technique and used together with gene array analysis has revolutionalized CNS molecular analysis. Here we describe the use of such...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-005-9008-4
更新日期：2006-02-01 00:00:00
abstract：:Planarians are rapidly developing into very useful research subjects in pharmacology and neuroscience research. Here we report that curare, a cholinergic nicotinic receptor antagonist, alleviates the nicotine-induced planarian seizure-like movements (pSLM) by up to 50 % at equimolar concentrations of nicotine and cura...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-015-1512-6
更新日期：2015-10-01 00:00:00
abstract：:The release of labeled norepinephrine from preloaded synaptosomes requires the presence of potassium and calcium. ATP-dependent binding of calcium to synaptic plasma membranes (SPM) may provide a means of maintaining the cation in a readily available pool for the triggering of transmitter release. A high Ca-binding ca...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965106
更新日期：1983-04-01 00:00:00
abstract：:The spontaneously hypertensive rat (SHR) has been proposed as an animal model for attention-deficit hyperactivity disorder (ADHD). The behavioural problems have been suggested to be secondary to altered reinforcement mechanisms in which nucleus accumbens dopaminergic activity plays an important role. Interaction betwe...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00973098
更新日期：1995-04-01 00:00:00
abstract：:Postmortem cerebral neocortical and hippocampal samples were taken from patients who died with dementia of the Alzheimer type (DAT) and individuals without diagnoses of neurological or psychiatric disease (control). Nicotinic binding was assayed with 20 nM [3H]acetylcholine [( 3H]ACh) in the presence of atropine, or w...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964952
更新日期：1989-08-01 00:00:00
abstract：:Acupuncture and moxibustion are traditional medical treatments that have come to play important roles in complementary and alternative medicines. Moxibustion also has a long history as a folk remedy in Japan, particularly due to the technical simplicity and selective efficacy on certain types of disease and distress. ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-005-8839-3
更新日期：2005-12-01 00:00:00
abstract：:The abnormal function of the voltage-gated potassium channel Kv10.2 can induce epilepsy. However, the physiological function of Kv10.2 in the central nervous system remains unclear. In this study, we found that Kv10.2 knockout (KO) increased the complexity of neurons in the CA3 subarea of hippocampus. Kv10.2 KO led to...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-020-03143-7
更新日期：2020-12-01 00:00:00
abstract：:Alterations in lysosomal proteases have been implicated in many neurodegenerative diseases. The current study demonstrates a concentration-dependent decrease in PC12 cell viability and transient changes in cystatin C (CYSC), cathepsin B (CATB), cathepsin D (CATD) and caspase-3 following exposure to H2O2. Furthermore, ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-007-9338-5
更新日期：2007-09-01 00:00:00
abstract：:Aneurysmal subarachnoid hemorrhage still has a high mortality and morbidity despite notable advances in surgical approaches to cerebral aneurysm (CA). We examined the role of aspirin in vascular inflammation and degeneration. CA was induced in male Sprague-Dawley rats by ligating left common carotid artery and bilater...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-015-1603-4
更新日期：2015-07-01 00:00:00
abstract：:The real-time iontophoretic method has measured volume fraction and tortuosity of the interstitial component of extracellular space in many regions and under different conditions. To interpret these data computer models of the interstitial space (ISS) of the brain are constructed by representing cells as Basic Cellula...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-019-02793-6
更新日期：2020-01-01 00:00:00
abstract：:Association of the candidate gene DLST with late-onset Alzheimer's disease (LOAD) risk has been suggested on the basis of case-control studies. This gene, located on chromosome 14q24.3, encodes a subunit of a mitochondrial component known to be defective in AD, the alpha-ketoglutarate dehydrogenase complex. Positive r...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/b:nere.0000014833.54481.1d
更新日期：2004-03-01 00:00:00
abstract：:Age-related increase of reactive oxygen species (ROS) is particularly detrimental in postmitotic tissues. Calorie restriction (CR) has been shown to exert beneficial effects, consistent with reduced ROS generation by mitochondria. Many antioxidant compounds also mimic such effects. N-acetyl cysteine (NAC) provides thi...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-005-6867-7
更新日期：2005-06-01 00:00:00
abstract：:Reserpine evokes transsynaptic impulse activity by depleting catecholaminergic neurotransmitters in the rat brain. Previous studies suggest a relationship between catecholaminergic activity and calmodulin concentration. In this report we employ Northern blot analysis to examine the effect of a single subcutaneous inje...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF01474683
更新日期：1993-02-01 00:00:00
abstract：:To assess RNAi mediated inhibition of the expression of wt-DYT1 on H(2)O(2)-induced toxicity in NIH 3T3 cells and primary cortical neurons. To detect the function of wild-type Torsin A and the effect of SiRNA on the wt-DYT1 gene. The shRNA expression vector was constructed by ligating annealed complementary shRNA olig...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-010-0177-4
更新日期：2010-08-01 00:00:00
abstract：:The study provides detailed biochemical correlates to the common histopathological diagnoses in epilepsy. A dot immunobinding procedure was used for quantification of NSE, GFA, S-100, NCAM, NF 68 and NF 200. The material consisted of samples from 48 patients either selected for surgical treatment of partial epilepsy o...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00967255
更新日期：1993-04-01 00:00:00
