Abstract:
:A double ligand enzyme-linked immunosorbent assay (ELISA) has been developed to detect antibodies against brain tissue antigens in the sera of patients with neurological diseases. The sera were tested on human white matter homogenate. The technique consists of successive incubations with the human serum to be tested, rabbit immunoglobulin G (IgG) to human immunoglobulins (Ig), alkaline phosphate-labeled protein A and alkaline phosphatase substrate. This procedure has the advantage of increased sensitivity compared to the classical ELISA. Application of this procedure to the sera of patients with neurological diseases showed that the unspecific binding is very low and the results are reliable. Moreover the test allows the detection of antibodies to chemically different antigenic structures that can occur in a variety of neurological diseases.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Bologa L,Hofstetter W,Schonenberger K,Jacque C,Herschkowitz Ndoi
10.1007/BF00964651subject
Has Abstractpub_date
1985-04-01 00:00:00pages
469-81issue
4eissn
0364-3190issn
1573-6903journal_volume
10pub_type
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