Abstract：
:The binding of the enkephalin dimer [D-Ala2, Leu5-NH-CH2-]2 (DPE2) is characterized by its high affinity for receptors on NG108-15 hybrid cells, the affinity constant K = 4.7 X 10(9) M-1 is up to 8-fold that of monomers (0.6 to 1.0 X 10(9) M-1), and a maximal binding capacity equal to one half that of the monomers. Kinetic studies showed that DPE2 binds with a 2-fold higher rate, k1 = 6.3 X 10(7) M-1min-1, than monomers (2.4 to 3.8 X 10(7) M-1min-1), and dissociates at a slower rate than monomers. Dissociation of DPE2 was consistently bi- or multiphasic but increased about 12% only after 3 hr of dissociation in the presence of a large excess of unlabeled enkephalin. The dissociation kinetics of monomers varied with enkephalin and experimental conditions used. Consistent with the value for the maximal binding capacity, the kinetic studies are interpreted in support of the hypothesis that DPE2 binds by cross-linking two subunits of one receptor.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Krumins SA,Lutz RA,Costa T,Rodbard Ddoi
10.1007/BF00965208subject
Has Abstractpub_date
1986-06-01 00:00:00pages
839-50issue
6eissn
0364-3190issn
1573-6903journal_volume
11pub_type
杂志文章abstract：:The ketone body, β-hydroxybutyrate (βOHB), is metabolised by the brain alongside the mandatory brain fuel glucose. To examine the extent and circumstances by which βOHB can supplement glucose metabolism, we studied guinea pig cortical brain slices using increasing concentrations of [U-13C]D-βOHB in conjunction with [1...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-017-2228-6
更新日期：2017-06-01 00:00:00
abstract：:Protein kinase C (PKC) plays a key role in cellular events including proliferation, survival and differentiation. Our previous study showed the effect of phorbol 12-myristate 13-acetate (PMA), a PKC activator, inducing a decrease in retinal cells proliferation. This effect was mediated by muscarinic type 1 receptors (...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-008-9847-x
更新日期：2009-05-01 00:00:00
abstract：:Microvessels were isolated from a bovine cortex and the transport of glucose was investigated by using 2-deoxy-D-[3H]glucose (2-DG). The apparent Km for 2-DG transport was 118 microM and therefore indicates a significant high affinity for the substrate. The inhibition of 2-DG uptake by D-glucose showed an apparent Ki ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00963858
更新日期：1981-04-01 00:00:00
abstract：:The participation of voltage-sensitive Na+ channels (VSSC) on the changes on internal (i) Na+, K+, Ca2+, and on DA, Glu, and GABA release caused by different concentrations of 4-AP was investigated in striatum synaptosomes. TTX, which abolished the increase in Na(i) (as determined with SBFI), induced by 0.1 mM 4-AP on...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/b:nere.0000013737.17288.ce
更新日期：2004-02-01 00:00:00
abstract：:Cysteinyl leukotrienes (CysLTs) are potent pro-inflammatory and immune modulating lipid mediators involved in inflammatory diseases and were boosted in human brain after acute phase of cerebral ischemia. The antagonism of CysLTs receptors may offer protection against ischemic damage. Therefore it seemed interesting to...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-014-1478-9
更新日期：2015-01-01 00:00:00
abstract：:The patterns of cholesterol content in chick brain and liver were studied during embryonic development and compared with the variations in the specific activities of mevalonate-activating enzymes during the same period. Total cholesterol content in both embryonic chick brain and liver increased during incubation. The ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964728
更新日期：1983-06-01 00:00:00
abstract：:The effect of postdecapitative ischemia (5 min at 37 degrees) and hypoxia (5% O2, 95% N2, 30 min) on the distribution of protein radioactivity in the cellular fractions of guinea pig cerebral cortex was studied. Ischemic conditions resulted in the increase of total radioactivity level and protein content in the mitoch...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00963828
更新日期：1979-02-01 00:00:00
abstract：:Pre-eclampsia (PE) is a pregnancy complicated syndrome that affects multiple organs including the brain that continue post- delivery in both mother and the offspring. We evaluated the expression of oligodendrocytes in the brain of PE rat model through development as well as the cognitive changes and other behavioural ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-020-02969-5
更新日期：2020-04-01 00:00:00
abstract：:The original version of this article unfortunately contained a mistake. The spelling of the author Tommaso Ianniti was incorrect and has been corrected as Tommaso Iannitti. The original article has been corrected. ...
journal_title：Neurochemical research
pub_type： 杂志文章,已发布勘误
doi：10.1007/s11064-018-2468-0
更新日期：2018-02-01 00:00:00
abstract：:One pathway by which tryptophan is metabolized in the brain as well as in the periphery is through cleavage of the indole ring to formylkynurenine and then kynurenine. Indoleamine-2,3-dioxygenase, the enzyme that catalyzes this cleavage, and kynurenine are distributed all across the different anatomic regions of brain...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/BF00964611
更新日期：1980-03-01 00:00:00
abstract：:Recent evidence suggests that young rodents submitted to high fructose (FRU) diet develop metabolic, and cognitive dysfunctions. However, it remains unclear whether these detrimental effects of FRU intake can also be observed in middle-aged mice. Nine months-old C57BL/6 female mice were fed with water (Control) or 10%...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-020-03135-7
更新日期：2020-12-01 00:00:00
abstract：:NMDA, the specific agonist of glutamate gated ion channels permeable to calcium, is implicated as a causal factor in the pathogenesis of several neurobiological disorders such as stroke, seizures, ischemia, and chronic neurodegenerative diseases. On the other hand, evidence on the roles of oxidative mechanisms involve...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF02533102
更新日期：1996-12-01 00:00:00
abstract：:Mice infected with the Venezuelan equine encephalomyelitis virus showed a significant decrease in the GABA content of cerebral hemispheres. Activity of the enzyme which synthetizes GABA, glutamate decarboxylase, is also reduced in whole cerebral hemispheres, neostriatum, and frontal cortex of infected animals, as comp...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964333
更新日期：1980-02-01 00:00:00
abstract：:We have previously reported that angiotensin receptor blockade reduces reperfusion hemorrhage in a suture occlusion model of stroke, despite increasing matrix metalloproteinase (MMP-9) activity. We hypothesized that candesartan will also decrease hemorrhage associated with delayed (6 h) tissue plasminogen activator (t...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-013-1185-y
更新日期：2013-12-01 00:00:00
abstract：:Nerve growth factor (NGF) has been isolated from human placental tissue. Using the chicken embryo dorsal root ganglia assay, we determined levels of NGF activity for the amnion, placental cotyledons, cord serum, fetal serum, and maternal serum. The highest levels of NGF activity were measured in placental cotyledons. ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964058
更新日期：1978-04-01 00:00:00
abstract：:In this review properties of lipid acetyltransferase enzymes are outlined. The three activities of interest are lyso PAF acetyltransferase (acetyl CoA: 1-alkyl-sn-glycero-3-phosphocholine acetyltransferase), AGP acetyltransferase (acetyl CoA: 1-alkyl sn-glycero-3-phosphate acetyltransferase) and a transacetylase activ...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1023/a:1007567205078
更新日期：2000-05-01 00:00:00
abstract：:Exposure to Hg2+ below 10 micrometer destroys synaptosomal membrane-associated sialidase of bovine brain in situ. Inhibition by Cu2+ occurs only at relatively higher concentrations, and is demonstrable after the synaptosomal membrane preparation has been presaturated with Cu2+ . Pb2+ does not inhibit enzymatic activit...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964363
更新日期：1978-02-01 00:00:00
abstract：:Since 1973, multiple effects of basic fibroblast growth factor have been described in a large number of cells. These effects include proliferation, survival and differentiation. The aim of this work was to study the intracellular pathways involved in the basic fibroblast growth factor (FGF2) effect on rat retinal cell...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-007-9491-x
更新日期：2008-05-01 00:00:00
abstract：:Rats fed a vitamin E-deficient diet for 7--8 weeks postweaning showed no change in brain weight or the activity in brain of various enzymes involved in neurotransmitter synthesis and metabolism. Body and muscle weights were markedly reduced. Muscle choline acetyltransferase and acetylcholinesterase activities were sig...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966003
更新日期：1978-12-01 00:00:00
abstract：:Uridine, a pyrimidine nucleoside, has been proposed to be a potential signaling molecule in the central nervous system. The understanding of uridine release in the brain is therefore of fundamental importance. The present study was performed to determine the characteristics of basal and morphine-induced uridine releas...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-012-0903-1
更新日期：2013-01-01 00:00:00
abstract：:The kinetics of the uptake of p-tyramine, m-tyramine, and dopamine were investigated in slices of the hypothalamus and striatum of the rat in the presence of nialamide. When uptake was analyzed by a least-squares fit to a Lineweaver-Burk plot, each amine appeared to be concentrated by both a "low"-affinity and a "high...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964440
更新日期：1979-10-01 00:00:00
abstract：:Using a model of middle cerebral artery occlusion (MCAO), we have previously demonstrated that treadmill exercise promotes angiogenesis in the ischemic penumbra through caveolin-1/VEGF signaling pathways. However, the function of caveolin-1/VEGF signaling in neurogenesis after MCAO has not been determined. In this stu...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-016-2081-z
更新日期：2017-02-01 00:00:00
abstract：:Mouse isolated vas deferens preincubated with [3-H]noradrenaline was superfused and the effect of alpha 1-adrenoceptor agonists was studied on the release of total radioactivity ([3H]noradrenaline + 3H-metabolites) and [3H]noradrenaline. Reverse phase high pressure liquid chromatography (HPLC) combined with scintillat...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965167
更新日期：1986-01-01 00:00:00
abstract：:Spinal cord injury (SCI), a severe health problem in worldwide, was commonly associated with functional disability and reduced quality of life. As the expression of brain-derived neurotrophic factor (BDNF) was substantial event in injured spinal cord, we hypothesized whether BDNF-overexpression could be in favor of th...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-016-1970-5
更新日期：2016-10-01 00:00:00
abstract：:DNase I sensitivity experiments were performed utilizing DNA probes to genes which are either transcribed in rat cortical neurons (the 68 kDa neurofilament gene and the neuron-specific enolase gene) or are transcriptionally silent (albumin). Results suggest that unlike liver, in which a hierarchy in chromatin conforma...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00969627
更新日期：1989-02-01 00:00:00
abstract：:Disruptive effects of calcium upon neurofilaments and glial filaments were studied in white matter of rat optic nerve and spinal cord and in rat peripheral nerve. Filament ultrastructure and tissue protein composition were compared following a calcium influx into excised tissues. A calcium influx was induced by freeze...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964040
更新日期：1981-03-01 00:00:00
abstract：:A prominent feature of brain pathology in Alzheimer's disease is a robust activation of the neuronal lysosomal system and major cellular pathways converging on the lysosome, namely, endocytosis and autophagy. Recent studies that identify a disturbance of the endocytic pathway as one of the earliest known manifestation...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1023/a:1007675508413
更新日期：2000-10-01 00:00:00
abstract：:Schwann cell biosynthesis of glycolipids was studied by in vitro incorporation of [3H]galactose into neonatal rat sciatic nerves before and after endoneurial explant culture and in culture of purified Schwann cells. In neonatal nerves prior to culture, [3H]galactose was actively incorporated into galactocerebrosides (...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00968672
更新日期：1990-03-01 00:00:00
abstract：:The expression and localization of syntaxin isoforms 1A and 1B in adrenergic and noradrenergic chromaffin cells were examined by both immunoblot analysis and confocal immunofluorescence microscopy. Syntaxin 1A was found in higher levels in noradrenergic cells, whereas syntaxin 1B was similarly expressed in most noradr...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1025658005937
更新日期：2003-10-01 00:00:00
abstract：:Depression afflicts more than 300 million people worldwide, but there is currently no universally effective drug in clinical practice. In this study, chronic restraint stress (CRS)-induced mice depression model was used to study the antidepressant effects of resveratrol and its mechanism. Our results showed that resve...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-020-03200-1
更新日期：2021-01-03 00:00:00
