Abstract:
:The participation of voltage-sensitive Na+ channels (VSSC) on the changes on internal (i) Na+, K+, Ca2+, and on DA, Glu, and GABA release caused by different concentrations of 4-AP was investigated in striatum synaptosomes. TTX, which abolished the increase in Na(i) (as determined with SBFI), induced by 0.1 mM 4-AP only inhibited by 30% the rise in Na(i) induced by 1 mM 4-AP. One millimolar 4-AP markedly decreased the fluorescence of the K+ indicator dye PBFI but 0.1 mM 4-AP did not. Like 1 mM 4-AP, ouabain decreased PBFI fluorescence and increased a considerable fraction of Na(i) in a TTX-insensitive manner. In contrast with the different TTX sensitivity of the rise in Na(i) induced by 0.1 and 1 mM 4-AP, the rise in Ca(i) (as determined with fura-2) induced by the two concentrations of 4-AP was markedly inhibited by TTX, as well as by omega-agatoxin in combination with omega-conotoxin GVIA, indicating that only the TTX-sensitive fraction of the rise in Na(i) induced by 4-AP is linked with the activation of presynaptic Ca2+ channels. It is concluded that the TTX-sensitive fraction of neurotransmitter release evoked by 4-AP is released by exocytosis, and the TTX insensitive fraction involves reversal of the neurotransmitters transporters. This contrasts with the exocytosis evoked by high K+ that is unchanged by TTX and with the neurotransmitter-transporter-mediated release evoked by veratridine, which is highly TTX sensitive and does not require activation of Ca2+ channels.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Galván E,Sitges Mdoi
10.1023/b:nere.0000013737.17288.cekeywords:
subject
Has Abstractpub_date
2004-02-01 00:00:00pages
347-55issue
2eissn
0364-3190issn
1573-6903journal_volume
29pub_type
杂志文章abstract::The effect of L-glutamate (Glu) and its structural analogs N-methyl-D-aspartate (NMDA), kainate (KA) and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA), on the activation of p42 mitogen activated protein kinase (MAPK) was examined in cultured chick radial glia cells, namely retinal Muller cells and cerebe...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1027345808746
更新日期:1997-06-01 00:00:00
abstract::Using the immunoblot technique, we found that an incubation of purified human myelin in 10 mM Tris-HCl buffer at pH 7.5 resulted in the conversion of the myelin-associated glycoprotein (MAG) to a smaller derivative (dMAG). Exogenously added 5 mM CaCl2 accelerated the conversion of MAG. In buffer containing more than 1...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964509
更新日期:1984-05-01 00:00:00
abstract::The current study investigated the neuroprotective activity of idebenone against pilocarpine-induced seizures and hippocampal injury in rats. Idebenone is a ubiquinone analog with antioxidant, and ATP replenishment effects. It is well tolerated and has low toxicity. Previous studies reported the protective effects of ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-014-1236-z
更新日期:2014-02-01 00:00:00
abstract::Systemic inflammation triggered by lipopolysaccharide (LPS) administration disrupts blood-brain barrier (BBB) homeostasis in animal models. This event leads to increased susceptibility of several encephalic structures to potential neurotoxicants present in the bloodstream. In this study, we investigated the effects of...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-018-2477-z
更新日期:2018-03-01 00:00:00
abstract::We previously reported that vanilloid receptor type 1 (VR1, or TRPV1) was up-regulated in dorsal root ganglion (DRG) and the spinal dorsal horn after chronic inflammatory pain produced by complete Freund's adjuvant (CFA) injection into the plantar of rat hind paw. In the present study, we found that subcutaneous or in...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-008-9751-4
更新日期:2008-10-01 00:00:00
abstract::A major part of the Ca-activated proteolytic activity in the soluble fraction from rabbit brain could be due to the activity of the neutral thiol-proteases calpain I and II. The activity of calpains exceeded that of the endogenous inhibitor, calpastatin, at all developmental stages studied. The level of calpains incre...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965622
更新日期:1989-11-01 00:00:00
abstract::Myelinating cells of both the peripheral and central nervous systems (CNSs) undergo dramatic cytoskeletal reorganization in order to differentiate and produce myelin. Myelinating oligodendrocytes in the CNS show a periodic actin pattern, demonstrating tight regulation of actin. Furthermore, recent data demonstrate tha...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-019-02753-0
更新日期:2020-03-01 00:00:00
abstract::Seizures are accompanied by an exacerbated activation of cerebral ion channels. 4-aminopyridine (4-AP) is a pro-convulsive agent which mechanism of action involves activation of Na(+) and Ca(2+) channels, and several antiepileptic drugs control seizures by reducing these channels permeability. The antidepressant, sert...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-016-1840-1
更新日期:2016-06-01 00:00:00
abstract::We propose the following scheme for cerebral uptake and overall metabolism of glucose in vivo: that brain selects from two pools of glucose anomers in arterial blood, that it takes up excess glucose, that glucose enters the brain tissue as glucose-6-phosphate through the actions of mutarotase and hexokinase, that some...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964568
更新日期:1985-02-01 00:00:00
abstract::Protein kinase A (PKA) plays a crucial role in tau hyperphosphorylation, an early event of Alzheimer disease (AD), and 17beta-estradiol replacement in aging women forestalls the onset of AD. However, the role of estradiol in PKA-induced tau hyperphosphorylation is not known. Here, we investigated the effect of 17beta-...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-008-9638-4
更新日期:2008-09-01 00:00:00
abstract::Hypothalamic and plasma dopamine-beta-hydroxylase [DBH, EC 1.14.2.1] activites were measured by a coupled radioenzymatic method. Animals representing five experimental groups [intact controls, adrenalectomized, pinealectomized, adrenalectomized pinealectomized, doubly sham-operated] were killed and sampled at 8 times ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965575
更新日期:1978-06-01 00:00:00
abstract::The first mentioning of the word "receptor" for the structure with which a bioactive compound should react for obtaining its specific influence on a physiological system goes back to the years around 1900. The receptor concept was adapted from the lock and key theory for the enzyme substrate and blockers interactions....
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-014-1398-8
更新日期:2014-10-01 00:00:00
abstract::Mice with a heterozygous knock-out of the myelin protein P0 gene (P0+/-) develop a neuropathy similar to human Charcot-Marie-Tooth disease. They are indistinguishable from wild-types (WT) at birth and develop a slowly progressing demyelinating neuropathy. The aim of this study was to investigate whether the regenerati...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-013-1030-3
更新日期:2013-06-01 00:00:00
abstract::Level of adenosine, an endogenous astrocyte-based neuromodulator, is primarily regulated by adenosine P1 receptors. This study assessed expression of adenosine P1 receptors, ADORA1 (adenosine A1 receptor) and ADORA2A (adenosine A2a receptor) and their association with glioma development and epilepsy in glioma patients...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-016-1893-1
更新日期:2016-07-01 00:00:00
abstract::Several epidemiological studies have shown that consumption of large quantities of vegetables especially cruciferous vegetables (Broccoli and Brussels sprouts) can protect against chronic diseases. Sulforaphane, an isothiocynate found in cruciferous vegetables has been demonstrated to have neuroprotective effects in s...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-016-1965-2
更新日期:2016-10-01 00:00:00
abstract::(E)-2-(1-hydroxyl-4-oxocyclohexyl) ethyl caffeine (HOEC), a naturally caffeic ester isolated from Incarvillea mairei, has been reported to possess anti-inflammatory activity by targeting 5-lipoxygenase. However, its other potential activities have yet to be explored. In this study, we measured antioxidant activity of ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-014-1498-5
更新日期:2015-03-01 00:00:00
abstract::Adult rabbit brain contains the enzymatic machinery to convert deoxyuridine to deoxyuridine triphosphate (dUTP). Although dUTP as dUMP can be readily incorporated into DNA in place of thymidine monophosphate, we detected no (3H)dUMP in newly synthesized (3H)DNA in adult rabbit brain after the intraventricular injectio...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965201
更新日期:1983-08-01 00:00:00
abstract::The activities of monoamine oxidase (MAO), catechol-O-methyltransferase (COMT) and gamma-aminobutyric acid transaminase (GABA-T) were measured in primary cultures from newborn rat cultivated from 6 different brain regions. These primary cultures contained mostly astroglial cells, evaluated by the presence of the glial...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00967658
更新日期:1984-01-01 00:00:00
abstract::Metabotropic glutamate receptors (mGluR) play a role in synaptic transmission, neuronal modulation and plasticity but their action in epileptic activity is still controversial. On the other hand adenosine acts as a neuromodulator with endogenous anticonvulsive properties. Since cerebellum from epileptic patients has s...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-006-9275-8
更新日期:2007-07-01 00:00:00
abstract::A sensitive and rapid assay method method for cysteic acid decarboxylase was develped which combined the selectivity of ion exchange resin (a complete retention of the substrate, cysteic acid, and exclusion of the product, taurine) with the speed of a vacuum filtration. The synthesis and purification of 35S-labeled cy...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964144
更新日期:1979-04-01 00:00:00
abstract::It has been reported that central dopaminergic system is implicated in the mechanism underlying general anesthesia. Whether dopamine (DA) neurons in midbrain ventral periaqueductal gray (vPAG) are involved in general anesthesia and how general anesthetics affect these neurons remain sparsely documented. To determine t...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-018-2486-y
更新日期:2018-04-01 00:00:00
abstract::The number of c-Fos protein-like immunoreactive (c-Fos-IR) neurons in the spinal dorsal horn evoked by noxious stimulation was previously shown to be increased following peripheral nerve injury, and this increase was proposed to reflect the neuropathic pain state. The aim of this study was to investigate whether anoma...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-014-1484-y
更新日期:2015-03-01 00:00:00
abstract::Sonic hedgehog (Shh), a secreted glycoprotein factor, can activate the Shh pathway, which has been implicated in neuronal polarization involving neurite outgrowth. However, little evidence is available about the effect of Shh on neurite outgrowth in primary cortical neurons and its potential mechanism. Here, we reveal...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-015-1736-5
更新日期:2016-04-01 00:00:00
abstract::Suspensions of rat brain microsomes, synaptosomes, and synaptic vesicles were able to convert adenosine to inosine by means of adenosine deaminase. Isosbestic points of this transformation, at 222, 250 and 281 nm, remained unchanged with time-course. This fact suggests that adenosine deaminase (ADA, E.C. 3.5.4.4) is l...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965316
更新日期:1986-04-01 00:00:00
abstract::In this study, changes of the expression of two mitochondrial and two nuclear genes encoding the subunits of cytochrome c oxidase (CO) and NADH dehydrogenase (ND) were studied in the hippocampus, inferior parietal lobule, and cerebellum of 10 Alzheimer's disease (AD) and 10 age-matched control subjects. The altered pr...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1020783614031
更新日期:1999-06-01 00:00:00
abstract::Previous studies have shown that chronic administration of SR-3 (a 1:4 mixture of alpha-linolenic and linoleic acid) affects spatial learning, thermoregulation, pain threshold and protection from seizures. The mode of action is unknown. One possible explanation is that the preparation induces changes in the fatty acid...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1022430620205
更新日期:1998-05-01 00:00:00
abstract::Microglia can exacerbate central nervous system disorders, including stroke and chronic progressive neurodegenerative diseases such as Alzheimer disease. Mounting evidence points to ion channels expressed by microglia as contributing to these neuropathologies. The Chloride Intracellular Channel (CLIC) family represent...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-013-1084-2
更新日期:2013-09-01 00:00:00
abstract::Previous studies have demonstrated that the c-Jun N-terminal kinase (JNK) pathway plays an important role in inducing neuronal apoptosis following cerebral ischemic injury. JNK signaling pathway in activated during cerebral ischemic injury. It participates in ischemia-induced neuronal apoptosis. However, whether JNK s...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-015-1753-4
更新日期:2016-04-01 00:00:00
abstract::Rat posterior eyecups containing the retina were prepared, loaded with [(3)H]glycine and superfused in order to determine its release originated from glycinergic amacrine cells and/or glial cells. Deprivation of oxygen and glucose from the Krebs-bicarbonate buffer used for superfusion evoked a marked increase of [(3)H...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-015-1713-z
更新日期:2016-02-01 00:00:00
abstract::The activity of gamma-glutamyltransferase localized in isolated brain synaptic membranes- and microvessels-enriched fractions was assayed after treatment of rats with either phenobarbital or ethanol. Phenobarbital increased the activity of gamma-glutamyltransferase in microvessels, without alteration of synaptic membr...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00993245
更新日期:1987-04-01 00:00:00