Abstract：
:The expression and localization of syntaxin isoforms 1A and 1B in adrenergic and noradrenergic chromaffin cells were examined by both immunoblot analysis and confocal immunofluorescence microscopy. Syntaxin 1A was found in higher levels in noradrenergic cells, whereas syntaxin 1B was similarly expressed in most noradrenergic and adrenergic cells. However, some heterogeneity was observed within each catecholaminergic phenotype. Although the majority of adrenergic cells appeared to express low levels of syntaxin 1A, about 7% was strongly stained for syntaxin 1A. A subpopulation of noradrenergic cells, about 17%, expressed greater levels of syntaxin 1B. Syntaxin 1B labeling showed a punctate appearance in the cytoplasm, whereas syntaxin 1A appeared predominantly localized to the plasma membrane. These data show differences in the exocytotic machinery of the two subtypes of chromaffin cells that may underlie some of the distinct characteristics of adrenaline and noradrenaline secretion.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Baltazar G,Carvalho AP,Duarte EPdoi
10.1023/a:1025658005937keywords:
subject
Has Abstractpub_date
2003-10-01 00:00:00pages
1453-7issue
10eissn
0364-3190issn
1573-6903journal_volume
28pub_type
杂志文章abstract：:Isozyme patterns of cholinesterase (ChE) from heart, tongue, and skeletal muscle of normal and dystrophic hamsters are presented. Two principal bands, bands 1 and 2, were evaluated. Band 1 migrates faster towards the anode than does band 2. While bands 1 and 2 stain for AChE and were found in control muscles, only ban...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964958
更新日期：1980-12-01 00:00:00
abstract：:Several studies showed that the up-regulation of glial glutamate transporter-1 (GLT-1) participates in the acquisition of brain ischemic tolerance induced by cerebral ischemic preconditioning or ceftriaxone pretreatment in rats. To explore whether GLT-1 plays a role in the acquisition of brain ischemic tolerance induc...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-011-0639-3
更新日期：2012-03-01 00:00:00
abstract：:Recent evidence reveals that prolactin gene expression (PRL-GE) in mammotropes occurs in pulses, but the molecular process(es) underlying this phenomenon remains unclear. Earlier, we have identified an E-box (E-box133) in the rat PRL promoter that binds several circadian elements and is critical for this dynamic proce...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-016-1843-y
更新日期：2016-06-01 00:00:00
abstract：:Spinal cord injury (SCI), a severe health problem in worldwide, was commonly associated with functional disability and reduced quality of life. As the expression of brain-derived neurotrophic factor (BDNF) was substantial event in injured spinal cord, we hypothesized whether BDNF-overexpression could be in favor of th...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-016-1970-5
更新日期：2016-10-01 00:00:00
abstract：:Analysis in mouse brain slices of the uptake of acetyl-L-[N-methyl-14C]carnitine with time showed it to be concentrative, and kinetic analysis gave a Km of 1.92 mM and a Vmax of 1.96 mumol/min per ml, indicating the presence of a low-affinity carrier system. The uptake was energy-requiring and sodium-dependent, being ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964865
更新日期：1989-05-01 00:00:00
abstract：:This study was designed to investigate the protective effects of extracellular superoxide dismutase (SOD3) against hydrogen peroxide (H2O2) induced damage in human neuroblastoma SH-SY5Y cells and to elucidate the mechanisms responsible for this beneficial effect. SOD3-overexpressing SH-SY5Y cells were generated by ade...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-016-1897-x
更新日期：2016-07-01 00:00:00
abstract：:The characteristics and properties of the increase in cytosolic [Ca2+] that occurs in bovine adrenal medullary chromaffin cells on exposure to angiotensin 11 have been investigated. In fura-2 loaded cells exposure to a maximally effective concentration of angiotensin II (100 nM) caused a rapid, but transient increase ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1024987128090
更新日期：2003-09-01 00:00:00
abstract：:One pathway by which tryptophan is metabolized in the brain as well as in the periphery is through cleavage of the indole ring to formylkynurenine and then kynurenine. Indoleamine-2,3-dioxygenase, the enzyme that catalyzes this cleavage, and kynurenine are distributed all across the different anatomic regions of brain...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/BF00964611
更新日期：1980-03-01 00:00:00
abstract：:Insulin-like growth factor 1 (IGF-1) stimulates α-secretase processing of amyloid precursor protein (APP) and decreases Aβ production. Little is known about the relationship between IGF-1 and β-site amyloid precursor protein cleaving enzyme 1 (BACE-1), the protease essential for the production of β-amyloid peptides (A...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-010-0260-x
更新日期：2011-01-01 00:00:00
abstract：:A simple, rapid method for determining depolarization-induced 45Ca influx into synaptosomes is described. Synaptosomes which had been depolarized in the presence of 45Ca were applied to a small column of Chelex-100 resin to separate free Ca2+ from that taken up by the tissue. Approximately 70% of the synaptosomal prot...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965062
更新日期：1982-02-01 00:00:00
abstract：:Intracerebral folate injections produce convulsions and brain lesions, folic acid itself and tetrahydrofolate being more potent toxins than 5-methyltetrahydrofolate, the primary folate of mammalian extracellular fluids. Folates are known to excite neurons, by unknown mechanisms Folates stimulate GTP binding and GTPase...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00973648
更新日期：1990-07-01 00:00:00
abstract：:This study examined changes in neuropeptide Y (NPY) Y2 receptor binding in the brains of C57BL/6 mice in response to different levels of high-fat diets via three dietary intervention methods: high-fat diet, switching from high- to low-fat diet and finally, energy restricted high-fat diet. Forty-five C57Bl/6 male mice ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-008-9661-5
更新日期：2008-09-01 00:00:00
abstract：:The Src family kinases are a family of intracellular, non-receptor tyrosine kinases that are involved in a variety of cellular functions including the regulation of inflammation and apoptosis after brain hypoxia. Caspase-1 (C1) activates IL-1β through the formation of complex structures, the inflammasomes, while caspa...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-015-1717-8
更新日期：2015-11-01 00:00:00
abstract：:Menadione (2-methyl-1,4-naphthoquinone) is a synthetic derivative of vitamin K that allows rapid redox cycling in cells and thereby generates reactive oxygen species (ROS). To test for the consequences of a treatment of brain astrocytes with menadione, we incubated primary astrocyte cultures with this compound. Incuba...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-019-02760-1
更新日期：2019-05-01 00:00:00
abstract：:Selective and limited proteolysis is a key step in the post-translational modification of peptide hormone precursors. This process appears to involve a proteolytic machinery including highly specific endoproteases. Some of the enzyme systems possibly involved in the processing of pro-neuropeptides will be described an...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/BF00966318
更新日期：1987-10-01 00:00:00
abstract：:Glutamine synthetase (GS) is the major glutamine-forming enzyme of vertebrates and is accepted to be a marker of astroglial cells. Maturation of astroglial cells is characterized by an increase of GS activity, and the regulation of this enzyme is the topic of many publications. Because of the fundamental role of the G...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-005-2754-5
更新日期：2005-05-01 00:00:00
abstract：:CNS trauma has been associated with an increase in free radical production, but the cellular sources of this increase or the mechanism involved in the production of free radicals are not known. We, therefore, investigated the effects of trauma on free radical production in cultured neurons, astrocytes and BV-2 microgl...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1014875210852
更新日期：2002-02-01 00:00:00
abstract：:Neuroprotective effects of metformin have been increasingly recognized in both diabetic and non-diabetic conditions. Thus far, no information has been available on the potential beneficial effects of metformin on peripheral nerve regeneration in diabetes mellitus. The present study was designed to investigate such a p...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-015-1803-y
更新日期：2016-05-01 00:00:00
abstract：:The inflammatory response mediated by microglia in the central nervous system is closely related to epilepsy. Notch signaling plays an important role in the microglial activation during hypoxia. This study aimed to investigate whether Notch signaling is involved in microglial activation and subsequent inflammation-rel...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-018-2544-5
更新日期：2018-06-01 00:00:00
abstract：:A phosphoinositide-linked peptide response in cultured rat astrocytes was studied by measuring the accumulation of [3H]inositol phosphates in the presence of lithium. Cultures derived from cortex, cerebellum and spinal cord each showed a unique pattern or degree of stimulation to a panel of neuropeptides. Cortical and...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00972490
更新日期：1988-04-01 00:00:00
abstract：:CNTF (ciliary neurotrophic factor) has been suggested to be an important survival factor for oligodendrocytes; however, this effect is inconsistently obtained and myelination appears normal in CNTF null animals. On the other hand, CNTF stimulates astrocytes to produce growth and trophic factors. Therefore, we tested t...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-006-9151-6
更新日期：2007-02-01 00:00:00
abstract：:The abnormal function of the voltage-gated potassium channel Kv10.2 can induce epilepsy. However, the physiological function of Kv10.2 in the central nervous system remains unclear. In this study, we found that Kv10.2 knockout (KO) increased the complexity of neurons in the CA3 subarea of hippocampus. Kv10.2 KO led to...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-020-03143-7
更新日期：2020-12-01 00:00:00
abstract：:Pig brain cerebral cortex was subfractionated by isopycnic centrifugation in sucrose gradients. In each subfraction the content of the agonist [3H]R-PIA binding, the activity of adenosine metabolizing enzymes (5'-nucleotidase and adenosine deaminase) and the activity of membrane marker enzymes were determined. The fra...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966790
更新日期：1992-02-01 00:00:00
abstract：:Tanshinone I (TsI) is an important lipophilic diterpene extracted from Danshen (Radix Salvia miltiorrhizae) and has been used in Asia for the treatment of cerebrovascular diseases such as ischemic stroke. In this study, we examined the neuroprotective effect of TsI against ischemic damage and its neuroprotective mecha...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-014-1312-4
更新日期：2014-07-01 00:00:00
abstract：:Clinical studies have demonstrated that decreased adiponectin is associated with the development of Type 2 diabetes mellitus (T2DM) and Alzheimer's disease (AD). We focused on determining the neuroprotective effect offered by adiponectin against streptozotocin-induced brain damage in ICV-STZ rat model. We found that a...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-017-2426-2
更新日期：2018-02-01 00:00:00
abstract：:The primary objective of this investigation was to assess the neuroprotective efficacy of spice active principles namely Eugenol (Eug) and isoeugenol (IE) in an acrylamide (ACR) neuropathy model in rats. In the present study, ACR administration (50 mg/kg bw, i.p. 3 times/week) for 5 weeks to growing rats caused typica...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-012-0924-9
更新日期：2013-02-01 00:00:00
abstract：:The dorsal raphe nucleus (DRN) has been suggested playing an important role in the pathophysiology of post-traumatic stress disorder (PTSD), however the underlying cellular mechanisms are not fully understood. The endoplasmic reticulum (ER) is a critical organelle for synthesis of membrane and secretory proteins, and ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-014-1262-x
更新日期：2014-04-01 00:00:00
abstract：:The anterograde axonal transport of choline-phosphoglycerides was studied in sciatic nerve motoneurons of adult (3-month-old) and aged (24-month-old) rats. After the spinal cord injection of [2-3H]glycerol, choline-phosphoglycerides; the major phospholipid class was transported along the nerve. The axonal transport ra...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00971365
更新日期：1987-01-01 00:00:00
abstract：:The microdialysis technique was used to study the effect of nitric oxide synthase (NOS) activity on taurine release. Taurine release was characterized in rat striatum that was excitotoxically lesioned compared to normal conditions. The basal taurine level of the dialysate decreased during quinolinate (QUIN) lesion in ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1022418231496
更新日期：1998-04-01 00:00:00
abstract：:Spinal cord injury (SCI) is a type of long-term disability with a high morbidity rate in clinical settings for which there is no effective clinical treatment to date. Usually, lithium is used as a popular mood stabilizer. Recently, growing evidence has shown that lithium has clear neuroprotective effects after SCI, an...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-018-2488-9
更新日期：2018-04-01 00:00:00
