Abstract：
:Selective and limited proteolysis is a key step in the post-translational modification of peptide hormone precursors. This process appears to involve a proteolytic machinery including highly specific endoproteases. Some of the enzyme systems possibly involved in the processing of pro-neuropeptides will be described and their mechanism of action discussed. Special emphasis will be on the following: i) the physico-chemical characteristics of proteolytic enzymes which are believed to be involved in the processing of some of these polypeptide hormone precursors; ii) the bio-specificity of these enzymes toward the substrates; iii) the importance of both secondary and tertiary structures of the cleavage domain in recognition by the selective proteases. These properties will be discussed in connection with the possible importance of the maturation enzymes in the in vivo regulation of hormone biosynthesis.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Gluschankof P,Cohen Pdoi
10.1007/BF00966318subject
Has Abstractpub_date
1987-10-01 00:00:00pages
951-8issue
10eissn
0364-3190issn
1573-6903journal_volume
12pub_type
杂志文章,评审abstract：:Dual-specificity tyrosine-regulated kinase 2 (DYRK2), a protein kinase that phosphorylates its substrates on serine/threonine, is expressed in numerous human tumors, but little is known about its role in the pathophysiology of glioma. In this study, we made an effort to explore the expression and function in human gli...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-017-2345-2
更新日期：2017-11-01 00:00:00
abstract：:Many studies have demonstrated that apoptosis play an important role in cerebral ischemic pathogenesis and may represent a target for treatment. Neuroprotective effect of quercetin has been shown in a variety of brain injury models including ischemia/reperfusion. It is not clear whether BDNF-TrkB-PI3K/Akt signaling pa...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-012-0871-5
更新日期：2012-12-01 00:00:00
abstract：:We estimated catecholamine levels in CSF of 15 epileptics and 75 non-neurological patients utilizing a high performance liquid chromatograph with a highly sensitive fluorometer and found the following results: The dopamine (DA) levels in males were significantly higher than those in females, while norepinephrine (NE) ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965900
更新日期：1982-10-01 00:00:00
abstract：:Crayfish glutamic acid decarboxylase (GAD), like the homologous enzymes from other species, is inhibited by carbonyl-trapping agents (e.g. aminooxyacetic acid; AOAA) and sulfhydryl reagents (e.g. 5,5'-dithiobis-(2-nitrobenzoic acid); DTNB). It also is inhibited by the product GABA, many anions (e.g. SCN- and Cl-), and...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964526
更新日期：1984-07-01 00:00:00
abstract：:A variety of naturally occurring amino acids, their isomers, and synthetic analogs were tested for their ability to inhibit uptake of [3H]glutamate into presynaptic vesicles from bovine cerebral cortex. Strongest inhibition (Ki < 1mM) was observed for trans-1-aminocyclopentane-1,3-dicarboxylic acid (t-ACPD) and erythr...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966925
更新日期：1993-01-01 00:00:00
abstract：:It is an honour to have this opportunity write an article in recognition of the immense contributions of Bruce Ransom to the field of glial research. For me (BAM) personally there are many highlights both as a colleague and a friend that come to mind when I reflect on the many years that I have known Bruce. My own ent...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-017-2315-8
更新日期：2017-09-01 00:00:00
abstract：:The extracellular amino acid content was measured in the parietal cortex in portacaval and sham operated rats, using the brain dialysis technique. The amino acid content of the perfusate was determined for 10 min before and during stimulation with potassium chloride. Basal levels of aspartate, glutamine, glycine, meth...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00972136
更新日期：1987-03-01 00:00:00
abstract：:These studies were designed to compare the effects of nitric oxide (NO) generating compounds with those of several iron containing compounds which do not generate NO on glutamate receptor function. Stimulation of primary cultures of cerebellar granule cells with N-methyl-D-aspartate (NMDA) or kainate results in the el...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00969689
更新日期：1995-07-01 00:00:00
abstract：:This study investigated the effects of aging on the actions of a specific glutamate reuptake blocker, L-trans-pyrrolidine-2, 4-dicarboxylic acid (PDC), in extracellular glutamate and glutamine in striatum of the awake rat. Microdialysis experiments were performed on young (2-3 months), middle-aged (12-14 months), aged...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1007624531077
更新日期：2001-01-01 00:00:00
abstract：:The Editors have retracted this article [1] following an investigation conducted by the journal. After publication concerns were raised regarding interpretation of the data presented in Fig. 4. The Editors requested additional data and clarification to confirm interpretation of data results. After further review, the ...
journal_title：Neurochemical research
pub_type： 杂志文章,撤回出版物
doi：10.1007/s11064-020-03077-0
更新日期：2020-09-01 00:00:00
abstract：:Human dental follicle cells (DFCs) are ectomesenchymal multipotent stem cells that form spheroid cell clusters (SCCs) under serum free medium cell culture conditions (SFM). Until today, molecular mechanisms for the formation of SCCs are unknown. In this study a quantitative phosphoproteomics approach revealed regulate...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-014-1298-y
更新日期：2014-07-01 00:00:00
abstract：:It has been established that oligodendrocytes, the myelin forming cells, participate in iron homeostasis through the synthesis and secretion of transferrin. Here we investigated whether a correlation exists between myelination, the commonly studied function of oligodendrocytes, and that of transferrin synthesis and se...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-004-1826-2
更新日期：1999-02-01 00:00:00
abstract：:This study was designed to investigate the effects of chronic toluene inhalation in high concentration on lipid peroxidation, antioxidant enzyme activities and ultrastructural changes in the sciatic nerves of rats. Male Wistar albino rats (150-250 g) were divided in two experimental groups: the control and the toluene...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-004-9683-6
更新日期：2005-01-01 00:00:00
abstract：:Using the replication-defective adenovirus vector, we overexpressed rat glia maturation factor (GMF) in primary astrocyte cultures derived from embryonic rat brains. Among the three isoforms of MAP kinase, there was a big increase in the phosphorylation of p38, as detected with Western blotting using the phosphospecif...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1014241300179
更新日期：2001-12-01 00:00:00
abstract：:The specific molecular events that underlie the age-related loss of cognitive function are poorly understood. Although not experimentally substantiated, age-dependent neuronal loss has long been considered central to age-related cognitive decline. More recently, age-related changes in brain white matter have taken pre...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-007-9341-x
更新日期：2007-12-01 00:00:00
abstract：:This study evaluates whether physiological variables differentially affect the local synthesis of protein constituents of synapses in subcellular fractions containing pinched-off dendrites (synaptodendrosomes). Synaptodendrosomes were pulse-labeled in a medium containing 35S-methionine with 3 or 25 mM KCl and in the p...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF02527725
更新日期：1996-06-01 00:00:00
abstract：:The composition of retrogradely transported axonal proteins was examined by acrylamide gel electrophoresis and gel autoradiography in the experimental neuropathy induced in rats by p-bromophenylacetylurea (BPAU). Protein composition was normal during the early phase of retrograde transport but showed significant abnor...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966781
更新日期：1993-06-01 00:00:00
abstract：:Berberine and palmatine exhibit a mild and competitive inhibition on bovine adrenal tyrosine hydroxylase (EC 1.14.16.2; TH). In this study, the inhibitory effects of protoberberine alkaloids (such as berberine, palmatine and coptisine) on dopamine biosynthesis in PC12 cells were investigated. Treatment with berberine ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1007541020736
更新日期：2000-03-01 00:00:00
abstract：:Antibodies to synthetic myelin basic protein peptide S82 (TTHYG-SLPQKAQGHRPQDEG) did not react with synthetic peptide S8 (GSLPQKAQGHRPQDENG) and only partially so with synthetic peptide S79 (AQGHRPQDEG); however, the antibodies did react to a considerable extent with an equimolar mixture of S8 and S79. Since the anti-...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964671
更新日期：1984-10-01 00:00:00
abstract：:Myelinated axons were isolated by flotation from bovine pons, middle cerebellar peduncle, cervical spinal cord and three regions of the subcortical white matter. The myelinated axons were osmotically and mechanically shocked, followed by fractionation on a linear 15% sucrose to 45% sucrose density gradient. Axolemma-e...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF01268880
更新日期：1988-05-01 00:00:00
abstract：:Malignant peripheral nerve sheath (MPNST) cell lines derived from patients with neurofibromatosis type 1 (NF!) were found to have basal cAMP levels which are two-fold higher than cAMP levels in normal human adult Schwann cells (nHSC). PCR analysis also revealed that normal adult human Schwann cells express mRNA for ty...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-011-0433-2
更新日期：2011-09-01 00:00:00
abstract：:The endogenous sialidase (N-acetylneuraminidase) activity of membranes prepared from goldfish retina and optic tectum displays characteristics similar to those reported for neural plasma membrane sialidases of other organisms. Endogenous membrane sialidase activity was found to be optimal at ph 4.0, and maximal releas...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965657
更新日期：1983-01-01 00:00:00
abstract：:Deep brain stimulation (DBS) of the pedunculopontine nucleus (PPN) has been proposed as a treatment strategy for gait disorder in patients with Parkinson's disease (PD). We thus performed a systematic review and meta-analysis of randomized and nonrandomized controlled trials to assess the effect of this treatment on g...
journal_title：Neurochemical research
pub_type： 杂志文章,meta分析
doi：10.1007/s11064-020-02962-y
更新日期：2020-04-01 00:00:00
abstract：:Adult rabbit brain contains the enzymatic machinery to convert deoxyuridine to deoxyuridine triphosphate (dUTP). Although dUTP as dUMP can be readily incorporated into DNA in place of thymidine monophosphate, we detected no (3H)dUMP in newly synthesized (3H)DNA in adult rabbit brain after the intraventricular injectio...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965201
更新日期：1983-08-01 00:00:00
abstract：:The long-acting opiate antagonist naltrexone hydrochloride was administered by intraperitoneal injection, in a dose response protocol, to adult rats. The drug was used to observe effects of opiate receptor blockade on cells of the pituitary gland and adjacent hypothalamus. At higher drug doses (5 mg/kg or 10 mg/kg), n...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964168
更新日期：1984-02-01 00:00:00
abstract：:Cholinergic muscarinic receptors, when stimulated by arecoline, can activate cytosolic phospholipase A(2) (cPLA(2)) to release arachidonic acid (AA) from membrane phospholipid. This signal can be imaged in the brain in vivo using quantitative autoradiography following the intravenous injection of radiolabeled AA, as a...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-007-9372-3
更新日期：2007-11-01 00:00:00
abstract：:The Src family kinases are a family of intracellular, non-receptor tyrosine kinases that are involved in a variety of cellular functions including the regulation of inflammation and apoptosis after brain hypoxia. Caspase-1 (C1) activates IL-1β through the formation of complex structures, the inflammasomes, while caspa...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-015-1717-8
更新日期：2015-11-01 00:00:00
abstract：:This study examined the relationship between the distance of axotomy and the death of injured retinal ganglion cells (RGCs) in adult hamsters and the relationship of cytochrome c and caspase-3 on the death pathway of RGCs. The left optic nerve (ON) of adult hamsters was transected either at 1 or 3 mm away from the opt...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-004-6889-6
更新日期：2004-11-01 00:00:00
abstract：:The effects of palmitate on intracellular and extracellular amino acid concentrations of cultured astrocytes was studied. Exposure of astrocytes to either 0.72 mM or 0.36 mM palmitate was associated with a significant reduction in the intracellular pool of glutamine and taurine. In contrast, the intracellular concentr...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF01000040
更新日期：1989-04-01 00:00:00
abstract：:Metabotropic glutamate receptors (mGluR) play a role in synaptic transmission, neuronal modulation and plasticity but their action in epileptic activity is still controversial. On the other hand adenosine acts as a neuromodulator with endogenous anticonvulsive properties. Since cerebellum from epileptic patients has s...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-006-9275-8
更新日期：2007-07-01 00:00:00
