Abstract：
:Human dental follicle cells (DFCs) are ectomesenchymal multipotent stem cells that form spheroid cell clusters (SCCs) under serum free medium cell culture conditions (SFM). Until today, molecular mechanisms for the formation of SCCs are unknown. In this study a quantitative phosphoproteomics approach revealed regulated phosphorylated proteins in SCCs, which were derived from DFCs after 24 and 48 h in SFM. These regulated proteins were categorized using the Kyoto encyclopedia of genes and genomes program. Here, cellular processes and signaling pathway were identified such as the focal adhesion kinase (FAK) signaling pathway. In addition to the phosphoproteomics approach we showed that a specific phosphorylation of FAK (Y397) was required for the formation of SCCs. In conclusion, this study disclosed the phosphoproteome of SCCs for the first time and showed that the FAK signaling pathway is required for the formation of SCCs.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Beck HC,Gosau M,Kristensen LP,Morsczeck Cdoi
10.1007/s11064-014-1298-ysubject
Has Abstractpub_date
2014-07-01 00:00:00pages
1199-205issue
7eissn
0364-3190issn
1573-6903journal_volume
39pub_type
杂志文章abstract：:Previous studies suggest that alterations of brain glutamate synthesis and release occur in experimental thiamine deficiency. In order to assess the integrity of post-synaptic glutamatergic receptors in thiamine deficiency, binding sites for [3H]glutamate (displaced by NMDA), [3H]-kainate, and [3H]quisqualate (AMPA si...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00995378
更新日期：1995-10-01 00:00:00
abstract：:A sensitive and rapid assay method method for cysteic acid decarboxylase was develped which combined the selectivity of ion exchange resin (a complete retention of the substrate, cysteic acid, and exclusion of the product, taurine) with the speed of a vacuum filtration. The synthesis and purification of 35S-labeled cy...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964144
更新日期：1979-04-01 00:00:00
abstract：:Ischemic brain injury continues to be of major concern in patients undergoing cardiopulmonary bypass (CPB) surgery for congenital heart disease. Striatum and hippocampus are particularly vulnerable to injury during these processes. Our hypothesis is that the neuronal injury resulting from CPB and the associated circul...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-014-1399-7
更新日期：2014-11-01 00:00:00
abstract：:Since 1973, multiple effects of basic fibroblast growth factor have been described in a large number of cells. These effects include proliferation, survival and differentiation. The aim of this work was to study the intracellular pathways involved in the basic fibroblast growth factor (FGF2) effect on rat retinal cell...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-007-9491-x
更新日期：2008-05-01 00:00:00
abstract：:The SAM strains, a group of related inbred strains consisting of senescence-prone inbred strains (SAMP) and senescence-resistant inbred strains (SAMR), have been successfully developed by selective inbreeding of the AKR/J strain of mice donated by the Jackson laboratory in 1968. The characteristic feature of aging com...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-009-9922-y
更新日期：2009-04-01 00:00:00
abstract：:The exposure to hypobaric hypoxia increased lipid peroxidation as indicated by thiobarbituric acid-reactive substances [TBARS] in rat brain. Plasma lactate/pyruvate ratio was used as a marker of hypoxia. We compared the protective effect of alpha-tocopherol with the effect of L-carnitine or phosphocreatine. Rats pretr...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1020339530924
更新日期：2002-09-01 00:00:00
abstract：:We previously showed that extracellular levels of N-acetylaspartate (NAA) increase when a medium with reduced NaCl concentration is perfused through a microdialysis probe, and proposed that NAA may be released during hypoosmotic swelling. Here, we demonstrate that this effect is due to hypoosmolarity of the perfusion ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1020778832745
更新日期：1998-08-01 00:00:00
abstract：:Abnormal myo-[2-3H]inositol incorporation into phosphatidylinositol has been found in phentolamine-treated synaptosomes that were isolated from the cerebral hemispheres of galactose toxic rats and incubated with [33P]Pi and myo-[2-3H] inositol. In galactose toxic rats phentolamine-stimulated myo-[2-3H]inositol labelin...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965068
更新日期：1982-01-01 00:00:00
abstract：:Transferrin (Tf), the iron-transport protein of vertebrate serum, is mainly synthesized in hepatocytes but is also found in other cell-types including oligodendrocytes. Our laboratory has characterized in a human oligodendrial cell line the presence of a new Tf transcript containing an alternative exon 1b replacing th...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1021692220482
更新日期：2002-11-01 00:00:00
abstract：:Propofol and ketamine may provide certain degree of neuroprotection, but the underlying mechanism remains unclear to date. The cAMP response element-binding protein (CREB) was proposed that its phosphorylation at Ser133 (P-CREB) constituted a convergence point involved in neuroprotection. The purpose of this study was...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-011-0582-3
更新日期：2012-01-01 00:00:00
abstract：:In order to develop a simplified method for long-term primary culture of highly-pure rat embryonic hippocampal neurons of low-density (10(3) cells/cm(2)), we optimized and modified conventional culturing methods. The modifications of our simplified method include: (1) combinational application of two growth substrates...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-010-0189-0
更新日期：2010-09-01 00:00:00
abstract：:Ubiquinone (coenzyme Q10), in addition to its function as an electron and proton carrier in mitochondrial electron transport coupled to ATP synthesis, acts in its reduced form (ubiquinol) as an antioxidant, inhibiting lipid peroxidation in biological membranes and protecting mitochondrial inner-membrane proteins and D...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1015591628503
更新日期：2002-05-01 00:00:00
abstract：:We examined the impact of peroxiredoxin-I (Prx-I) and peroxiredoxin-II (Prx-II) stable transduction on oxidative stress in PC12 neurons and NIH3T3 fibroblasts and found variability depending on cell type and Prx subtype. In PC12 neurons, Prx-II suppressed reactive oxygen species (ROS) generation by 36% (p < 0.01) rela...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1026670620633
更新日期：2000-12-01 00:00:00
abstract：:Turmeric has been in use since ancient times as a condiment and due to its medicinal properties. Curcumin, the yellow colouring principle in turmeric, is polyphenolic and major active constituent. Besides anti-inflammatory, thrombolytic and anticarcinogenic activities, curcumin also possesses strong antioxidant proper...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-007-9547-y
更新日期：2008-06-01 00:00:00
abstract：:The recent publication of the human genome sequence provides an opportunity both to combat diseases that are presently considered as pharmaceutically intractable and also to improve current therapies for many common human diseases. The identification of every human gene by ongoing bioinformatic efforts has the potenti...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1023/a:1022349805831
更新日期：2003-02-01 00:00:00
abstract：:The calcium-activated phosphodiesteratic hydrolysis of 32P-labeled phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 4-phosphate in prelabeled nerve ending membranes is inhibited by the aminoglycosides neomycin and gentamicin, and to a lesser extent, by streptomycin. The inhibition is overcome by increasi...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965878
更新日期：1985-08-01 00:00:00
abstract：:A role for protein phosphorylation in the process of neurite outgrowth has been inferred from many studies of the effects of protein kinase inhibitors and activators on cultured neurotumor cells and primary neuronal cells from developing brain or ganglia. Here we re-examine this issue, using a culture system derived f...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1021916509858
更新日期：1997-10-01 00:00:00
abstract：:The activity of gamma-glutamyltransferase localized in isolated brain synaptic membranes- and microvessels-enriched fractions was assayed after treatment of rats with either phenobarbital or ethanol. Phenobarbital increased the activity of gamma-glutamyltransferase in microvessels, without alteration of synaptic membr...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00993245
更新日期：1987-04-01 00:00:00
abstract：:Postmortem changes in the activity of GDH, GAD, GABA-T, and GS in the adult forebrain and cerebellum and in the forebrain of newborns have been investigated. The activities of GDH, GAD, and GS were stable till 12 hr after death in both adult and young brain regions studied. The activity of GABA-T was stable till 30 mi...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966226
更新日期：1986-10-01 00:00:00
abstract：:The activity, properties, and developmental pattern of cysteine sulfinate transaminase (CSA-T) were studied in chick retina and compared with the activity, properties, and developmental pattern of glutamate oxaloacetate transaminase (GOT). Their optimum pH is identical whereas the effect of pyridoxal phosphate seems t...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964358
更新日期：1978-02-01 00:00:00
abstract：:Three peptides overlapping the tryptophan region of bovine CNS myelin basic protein were synthesized by the solid phase procedure of Merrifield. These were the nonapeptide H-Phe-Ser-Trp-Gly-Ala-Glu-Gly-Gln-Lys-OH, the octapeptide H-Ser-Trp-Gly-Ala-Glu-Gly-Gln-Lys-OH, and the heptapeptide H-Trp-Gly-Ala-Glu-Gly-Gln-Lys-...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964458
更新日期：1980-01-01 00:00:00
abstract：:Protein kinase A (PKA) plays a crucial role in tau hyperphosphorylation, an early event of Alzheimer disease (AD), and 17beta-estradiol replacement in aging women forestalls the onset of AD. However, the role of estradiol in PKA-induced tau hyperphosphorylation is not known. Here, we investigated the effect of 17beta-...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-008-9638-4
更新日期：2008-09-01 00:00:00
abstract：:Müller cells, the predominant glial cells in the retina, are thought to play important roles in the survival of retinal neurons. Previous studies have demonstrated that Müller cells express brain-derived neurotrophic factor (BDNF), which has a pronounced neurotrophic effect on retinal ganglion cells. In this study, we...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-005-7936-7
更新日期：2005-09-01 00:00:00
abstract：:Myelinated axons were isolated by flotation from bovine pons, middle cerebellar peduncle, cervical spinal cord and three regions of the subcortical white matter. The myelinated axons were osmotically and mechanically shocked, followed by fractionation on a linear 15% sucrose to 45% sucrose density gradient. Axolemma-e...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF01268880
更新日期：1988-05-01 00:00:00
abstract：:The metabolic effects of ganglioside GM1 were found to be quite different in brain synaptosomes and phagocytic cells. Incubation of rat brain cortex synaptosomes with GM1 was shown to decrease the production of reactive oxygen species induced by Fe2+-H2O2 system and measured by chemiluminometric method in the presence...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1020296605444
更新日期：2002-08-01 00:00:00
abstract：:Evidence is presented in support of the working hypothesis that "prenervous" neurotransmitters directly participate in cell-cell interactions occurring during the first several cleavage divisions of sea urchin embryos, a function which may occur during the early development of higher animals as well. This intercellula...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00963906
更新日期：1981-01-01 00:00:00
abstract：:The elevated plus maze (EPM) is an animal model of anxiety used to test the effects of anxioselective drugs. The loss of the anxiolytic effect of drugs during the second exposure to the EPM is called the "one trial tolerance" (OTT) phenomenon. The present study was designed to investigate the relationship between the ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-013-1051-y
更新日期：2013-07-01 00:00:00
abstract：:Populations of enriched glial precursor cells and astrocytes isolated from primary cultures of newborn rat brain were used to study the synthesis of sulfated glycoproteins. Both cell types incorporated [3H]glucosamine and [35S]sulfate into carbohydrate side chains of proteoglycans and glycoproteins. The rate of incorp...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965947
更新日期：1986-08-01 00:00:00
abstract：:Alzheimer's disease (AD) is the most common form of dementia in the aged population. Early-onset familial AD (FAD) involves mutations in a gene on chromosome 21 encoding the amyloid protein precursor or on chromosomes 14 or 1 encoding genes known as presenilins. All mutations examined have been found to increase the p...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1023/a:1022471729291
更新日期：1998-05-01 00:00:00
abstract：:We propose the following scheme for cerebral uptake and overall metabolism of glucose in vivo: that brain selects from two pools of glucose anomers in arterial blood, that it takes up excess glucose, that glucose enters the brain tissue as glucose-6-phosphate through the actions of mutarotase and hexokinase, that some...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964568
更新日期：1985-02-01 00:00:00
