Abstract:
:Müller cells, the predominant glial cells in the retina, are thought to play important roles in the survival of retinal neurons. Previous studies have demonstrated that Müller cells express brain-derived neurotrophic factor (BDNF), which has a pronounced neurotrophic effect on retinal ganglion cells. In this study, we investigated whether Müller cells express and release BDNF in culture. Reverse transcriptase-PCR, immunocytochemistry and Western blotting revealed that Müller cells produce BDNF mRNA and protein. Using the enzyme-linked immunosorbant assay, BDNF protein levels in Müller cells and their conditioned medium were quantified, demonstrating that Müller cells produce and release high levels of BDNF. Noradrenaline administration caused an upregulation of BDNF mRNA and protein expression by cultured Müller cells. These results suggest that Müller cells may act as an endogenous source of BDNF in the retina. Furthermore, induction of BDNF expression by adrenergic agonists may provide a therapeutic approach to retinal neurodegenerative disorders.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Seki M,Tanaka T,Sakai Y,Fukuchi T,Abe H,Nawa H,Takei Ndoi
10.1007/s11064-005-7936-7keywords:
subject
Has Abstractpub_date
2005-09-01 00:00:00pages
1163-70issue
9eissn
0364-3190issn
1573-6903journal_volume
30pub_type
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