Abstract:
:Ethyl pyruvate (EP) has recently been reported to afford protection against neurodegenerative disorders. However, the mechanism underlying EP-mediated neuroprotection remains to be elucidated. Because peroxynitrite has been extensively implicated in the pathogenesis of various forms of neurodegenerative disorders via its cytotoxic effects, this study was undertaken to investigate whether the neuroprotective effect of EP is associated with inhibition of peroxynitrite-induced DNA strand breaks, a critical event leading to peroxynitrite elicited cytotoxicity. Incubation of phiX-174 plasmid DNA with 3-morpholinosydnonimine (SIN-1), a peroxynitrite generator, led to the formation of both single- and double-stranded DNA breaks in a concentration- and time- dependent manner. The presence of EP (0.5-10 mM) was found to significantly inhibit SIN-1-induced DNA strand breaks in a concentration-dependent fashion. The consumption of oxygen induced by 250 microM SIN-1 was found to be decreased in the presence of EP (0.5-10 mM), indicating that EP might affect the auto-oxidation of SIN-1. It was observed that incubation of the plasmid DNA with authentic peroxynitrite caused significant DNA strand breaks, which could also be dramatically inhibited by EP (0.5-10 mM). EPR spectroscopy in combination with spin-trapping technique using 5,5-dimethylpyrroline-N- oxide (DMPO) as a spin trap demonstrated the formation of DMPO-hydroxyl radical adducts (DMPO-OH) from authentic peroxynitrite, and that EP at 0.5-10 mM inhibited the adduct signal in a concentration-dependent manner. Taken together, these results demonstrate for the first time that EP can inhibit peroxynitrite-mediated DNA damage and hydroxyl radical generation.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Chen W,Jia Z,Zhu H,Zhou K,Li Y,Misra HPdoi
10.1007/s11064-009-0059-9subject
Has Abstractpub_date
2010-02-01 00:00:00pages
336-42issue
2eissn
0364-3190issn
1573-6903journal_volume
35pub_type
杂志文章abstract::Deposition of amyloid beta peptide in human brain in the form of senile plaques is a neuropathological hallmark of Alzheimer's disease (AD). Levels of a phospholipid breakdown product, glycerophosphocholine (GPC), also increase in AD brain. The effect of GPC on amyloid beta(1-40) peptide (Abeta) aggregation in PBS buf...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-004-7036-0
更新日期:2004-12-01 00:00:00
abstract::Cell surface β-Amyloid precursor protein (APP) is known to have a functional role in iron homeostasis through stabilising the iron export protein ferroportin (FPN). Mechanistic evidence of this role has previously only been provided through transcriptional or translational depletion of total APP levels. However, numer...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-019-02747-y
更新日期:2019-06-01 00:00:00
abstract::The diversification of neuronal subtypes during corticogenesis is fundamental to the establishment of the complex cortical structure. Although subtype specification has been assumed to occur in neural progenitor cells, increasing evidence has begun to reveal the plasticity of subtype determination in immature neurons....
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-017-2441-3
更新日期:2018-01-01 00:00:00
abstract::Probenecid inhibits the transport of the small monocarboxylic acids lactate and propionate from blood to brain but does not affect the transport of 5HIAA or HVA. Neither lactate, 5HIAA, HVA, nor probenecid itself inhibits probenecid uptake into brain from blood and neither lactate nor 5HIAA itself inhibits 5HIAA uptak...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965898
更新日期:1982-10-01 00:00:00
abstract::The p53 tumor suppressor gene is a sequence-specific transcription factor that activates the expression of genes engaged in promoting growth arrest or cell death in response to multiple forms of cellular stress. p53 expression is elevated in damaged neurons in acute models of injury such as ischemia and epilepsy and i...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1023/a:1021687810103
更新日期:2003-01-01 00:00:00
abstract::The dorsal raphe nucleus (DRN) has been suggested playing an important role in the pathophysiology of post-traumatic stress disorder (PTSD), however the underlying cellular mechanisms are not fully understood. The endoplasmic reticulum (ER) is a critical organelle for synthesis of membrane and secretory proteins, and ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-014-1262-x
更新日期:2014-04-01 00:00:00
abstract::The steady-state mRNA levels of NGF, BDNF and NT-3, and the mRNA levels of their receptors p75NGFR, trk, trkB, and trkC were examined in various human peripheral neuropathies, to determine the correlation with myelinated fiber pathology and T cell and macrophage invasions in the diseased nerves. Steady state levels of...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1022434209787
更新日期:1998-06-01 00:00:00
abstract::Altered neurogenesis has been reported in Alzheimer disease (AD), the most common neurodegenerative disorder characterized with hyperphosphorylated tau and accumulation of β-amyloid (Aβ). Recent studies suggest that tau phosphorylation is essential for hippocampal neurogenesis, however, it is not known whether tau pho...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-010-0316-y
更新日期:2011-02-01 00:00:00
abstract::To evaluate the influence of light and darkness on brain pyroglutamyl-peptidase I (pGluPI) activity, four experimental groups of rats were compared at the same time-point (10.00 h). Two groups were designed with a standard 12-12 h light-dark cycle: In group A, the lights were on from 7.00 h to 19.00 h, and the experim...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1010996425408
更新日期:2001-05-01 00:00:00
abstract::CNTF (ciliary neurotrophic factor) has been suggested to be an important survival factor for oligodendrocytes; however, this effect is inconsistently obtained and myelination appears normal in CNTF null animals. On the other hand, CNTF stimulates astrocytes to produce growth and trophic factors. Therefore, we tested t...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-006-9151-6
更新日期:2007-02-01 00:00:00
abstract::There is increasing evidence that proteins normally involved in the cell cycle play a role in the regulation of neuronal apoptotic death following various insults. However, it is not clear if the same mechanisms regulate cell death of oligodendrocytes as well. In this study, we investigated the mechanism of ceramide-i...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/b:nere.0000021236.32785.37
更新日期:2004-05-01 00:00:00
abstract::A role for protein phosphorylation in the process of neurite outgrowth has been inferred from many studies of the effects of protein kinase inhibitors and activators on cultured neurotumor cells and primary neuronal cells from developing brain or ganglia. Here we re-examine this issue, using a culture system derived f...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1021916509858
更新日期:1997-10-01 00:00:00
abstract::Plasmalogens are ether-linked phospholipids that are abundant in nervous tissues. Their biological role is unclear, but may involve membrane structure/function and antioxidant activities. This study further investigates a recent report that chronic administration of myo-inositol in rats increased brain phosphatidyleth...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/b:nere.0000018859.23881.f9
更新日期:2004-04-01 00:00:00
abstract::Somatostatin contents of striatum, hippocampus, and pons medulla have been followed in two inbred strains of mice (C57B1/6J and Balb/cJ) with aging and long term alcohol exposure (over a 25 month period of intoxication). Marked strain dependent differences in basic levels of somatostatin and genotypic variations in re...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965332
更新日期:1986-05-01 00:00:00
abstract::The study provides detailed biochemical correlates to the common histopathological diagnoses in epilepsy. A dot immunobinding procedure was used for quantification of NSE, GFA, S-100, NCAM, NF 68 and NF 200. The material consisted of samples from 48 patients either selected for surgical treatment of partial epilepsy o...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00967255
更新日期:1993-04-01 00:00:00
abstract::We found that chronic lithium diet affects the sensitivity of neuroleptic receptors and the content of amino acids in the brain, and that the changes in adult animals differ from those in young rats. Pregnant rats were kept on lithium diet (pellets with 0.21% Li2CO3 and 0.21% NaCl) during the gestation period and the ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964703
更新日期:1983-05-01 00:00:00
abstract::Two models of perturbed cerebellar ontogenesis were obtained by a single administration of methylazoxymethanol (MAM), a potent antimitotic agent, to mouse pups either on the day of birth (MAM0 mice) or at postnatal day 5 (MAM5 mice). The alterations of the cerebellar GABAergic system were studied by measuring glutamic...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00968010
更新日期:1992-07-01 00:00:00
abstract::Turmeric has been in use since ancient times as a condiment and due to its medicinal properties. Curcumin, the yellow colouring principle in turmeric, is polyphenolic and major active constituent. Besides anti-inflammatory, thrombolytic and anticarcinogenic activities, curcumin also possesses strong antioxidant proper...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-007-9547-y
更新日期:2008-06-01 00:00:00
abstract::Serotonin (5HT) synthesis in brain is influenced by precursor (tryptophan (TRP)) concentrations, which are modified by food ingestion. Hence, in rats, a carbohydrate meal raises brain TRP and 5HT; a protein-containing meal does not, but little attention has focused on differences among dietary proteins. Recently, sing...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-010-0382-1
更新日期:2011-03-01 00:00:00
abstract::Schwann cell biosynthesis of glycolipids was studied by in vitro incorporation of [3H]galactose into neonatal rat sciatic nerves before and after endoneurial explant culture and in culture of purified Schwann cells. In neonatal nerves prior to culture, [3H]galactose was actively incorporated into galactocerebrosides (...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00968672
更新日期:1990-03-01 00:00:00
abstract::A sensitive and rapid assay method method for cysteic acid decarboxylase was develped which combined the selectivity of ion exchange resin (a complete retention of the substrate, cysteic acid, and exclusion of the product, taurine) with the speed of a vacuum filtration. The synthesis and purification of 35S-labeled cy...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964144
更新日期:1979-04-01 00:00:00
abstract::The cellular effects of eleven compounds including chalcone glycosides isolated from Brassica rapa L. 'hidabeni' and their synthetic derivatives were studied in rat pheochromocytoma PC12 cells. Of the compounds tested, 4'-O-β-D-glucopyranosyl-3',4-dimethoxychalcone (A2) significantly increased the levels of the phosph...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-013-1152-7
更新日期:2013-11-01 00:00:00
abstract::In this paper we propose a powerful procedure to measure functional activation of the mouse delta-opioid receptor transiently expressed in mammalian cells. Receptor stimulation was assessed using a population of electroporated COS cells, transfected at a 50% efficiency. Under those conditions, agonist-promoted activat...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF02532371
更新日期:1996-11-01 00:00:00
abstract::A prominent feature of brain pathology in Alzheimer's disease is a robust activation of the neuronal lysosomal system and major cellular pathways converging on the lysosome, namely, endocytosis and autophagy. Recent studies that identify a disturbance of the endocytic pathway as one of the earliest known manifestation...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1023/a:1007675508413
更新日期:2000-10-01 00:00:00
abstract::The present study has attempted to elucidate the alteration of serotonin turnover after 24 h REM sleep deprivation in different regions in brain of young rat. Sleep deprivation was induced by the inverted flower pot technique. Results of this study show increased serotonin turnover after 24 h REM sleep deprivation in ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-005-9139-7
更新日期:2006-01-01 00:00:00
abstract::Menadione (2-methyl-1,4-naphthoquinone) is a synthetic derivative of vitamin K that allows rapid redox cycling in cells and thereby generates reactive oxygen species (ROS). To test for the consequences of a treatment of brain astrocytes with menadione, we incubated primary astrocyte cultures with this compound. Incuba...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-019-02760-1
更新日期:2019-05-01 00:00:00
abstract::Tanshinone I (TsI) is an important lipophilic diterpene extracted from Danshen (Radix Salvia miltiorrhizae) and has been used in Asia for the treatment of cerebrovascular diseases such as ischemic stroke. In this study, we examined the neuroprotective effect of TsI against ischemic damage and its neuroprotective mecha...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-014-1312-4
更新日期:2014-07-01 00:00:00
abstract::Histidinemia is an inherited metabolic disorder biochemically characterized by high concentrations of histidine in biological fluids. Usually affected patients are asymptomatic although some individuals have mental retardation and speech disorders. Considering the high prevalence of histidinemia and the scarce informa...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-012-0703-7
更新日期:2012-05-01 00:00:00
abstract::The mechanism of focal demyelination in multiple sclerosis has been a long-standing enigma of this disorder. Cytokines, a diverse family of signalling molecules, are viewed as potential mediators of the process based on clinical observations and studies with animal models and tissue/cell culture systems. Myelin and ol...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1023/a:1022493013904
更新日期:1998-03-01 00:00:00
abstract::It is thought that changes in gene expression in the brain mediate chronic ethanol-induced complex behaviors such as tolerance, dependence, and sensitization, and also relate to ethanol-induced brain toxicity. Using high-density filter-based cDNA microarrays (GeneFilters), we analyzed the expression of over 5000 genes...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1020937728506
更新日期:2002-10-01 00:00:00